##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938173_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3632168 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87315179253823 33.0 31.0 34.0 30.0 34.0 2 32.062102578955596 34.0 31.0 34.0 30.0 34.0 3 32.13108479563721 34.0 31.0 34.0 30.0 34.0 4 35.652363547060595 37.0 35.0 37.0 33.0 37.0 5 35.66534807861311 37.0 35.0 37.0 35.0 37.0 6 35.6926350873638 37.0 35.0 37.0 35.0 37.0 7 35.63023186152182 37.0 35.0 37.0 33.0 37.0 8 35.60653169126538 37.0 35.0 37.0 33.0 37.0 9 37.264728944255886 39.0 37.0 39.0 34.0 39.0 10-11 37.18560884298303 39.0 37.0 39.0 34.0 39.0 12-13 37.08346296206563 39.0 37.0 39.0 33.0 39.0 14-15 38.340372058781426 40.0 38.0 41.0 33.5 41.0 16-17 38.26291886829023 40.0 38.0 41.0 33.0 41.0 18-19 38.195864563533405 40.0 38.0 41.0 33.0 41.0 20-21 38.08349957931461 40.0 38.0 41.0 33.0 41.0 22-23 37.931795831029845 40.0 38.0 41.0 33.0 41.0 24-25 37.87474147671584 40.0 38.0 41.0 33.0 41.0 26-27 37.880629557883886 40.0 38.0 41.0 33.0 41.0 28-29 37.79019982005238 40.0 38.0 41.0 33.0 41.0 30-31 37.602585563222846 40.0 37.5 41.0 32.0 41.0 32-33 37.38438461546933 40.0 37.0 41.0 31.5 41.0 34-35 37.18032301919955 40.0 37.0 41.0 31.0 41.0 36-37 36.96466022496757 40.0 36.0 41.0 30.5 41.0 38-39 36.725702115100404 39.5 36.0 41.0 30.0 41.0 40-41 36.492183318613016 39.0 35.5 41.0 30.0 41.0 42-43 36.20477053924819 39.0 35.0 41.0 29.5 41.0 44-45 35.988415458756315 39.0 35.0 40.5 29.0 41.0 46-47 35.861094117893224 39.0 35.0 41.0 28.0 41.0 48-49 35.60600129179047 39.0 35.0 41.0 27.0 41.0 50-51 34.94846948158786 38.5 34.5 40.0 26.0 40.5 52-53 35.01464276982783 38.5 34.5 40.0 26.0 41.0 54-55 35.14066075688129 39.0 35.0 40.0 26.0 41.0 56-57 34.91009019957227 38.0 35.0 40.0 26.0 41.0 58-59 34.624639471522244 38.0 34.0 40.0 25.5 41.0 60-61 34.23003547743386 37.5 34.0 40.0 24.0 41.0 62-63 33.79222959951191 37.0 33.5 40.0 23.0 41.0 64-65 33.34549351792098 36.0 33.0 39.0 22.0 41.0 66-67 32.905985763874355 36.0 33.0 39.0 20.5 41.0 68-69 32.410292420394654 35.0 32.0 39.0 19.0 40.0 70-71 31.915903394336382 35.0 32.0 38.0 17.5 40.0 72-73 31.43539712370133 35.0 31.0 37.0 13.0 39.5 74-75 30.922510605236322 35.0 31.0 36.5 7.5 39.0 76-77 30.40600751396962 34.5 30.0 36.0 6.5 39.0 78-79 29.861834171767384 34.0 30.0 35.5 2.0 37.5 80-81 29.355982845507146 34.0 29.0 35.0 2.0 37.0 82-83 28.846639803004706 34.0 29.0 35.0 2.0 36.5 84-85 28.383288988835318 34.0 28.5 35.0 2.0 36.0 86-87 27.912052250887072 33.5 27.0 35.0 2.0 35.5 88-89 27.368668932714563 33.0 26.0 35.0 2.0 35.0 90-91 26.772548929454807 33.0 25.0 35.0 2.0 35.0 92-93 26.181660512399205 32.5 24.0 35.0 2.0 35.0 94-95 25.596677796841995 32.0 21.5 34.5 2.0 35.0 96-97 24.766213870063282 32.0 17.0 34.0 2.0 35.0 98-99 23.699899068545285 31.0 2.0 34.0 2.0 35.0 100-101 20.87443215732312 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3256879830921662 1101 2 0.25685182565102593 1101 3 0.18272842512423892 1101 4 0.18429273941967494 1101 5 0.17807936380940959 1101 6 0.1632373548073005 1101 7 0.19975328365629252 1101 8 0.13950836678338874 1101 9 0.13709745118355698 1101 10-11 0.22736722524030029 1101 12-13 0.10274030364388409 1101 14-15 0.17935102280185333 1101 16-17 0.2991824213957628 1101 18-19 0.26464081645453774 1101 20-21 0.31755085799647986 1101 22-23 0.38827179158844416 1101 24-25 0.37437019751218514 1101 26-27 0.2308053147885616 1101 28-29 0.374596456546314 1101 30-31 0.37560770025404366 1101 32-33 0.33485760057239844 1101 34-35 0.3288837580575148 1101 36-37 0.3537684930845586 1101 38-39 0.3716333209797327 1101 40-41 0.3272267479479609 1101 42-43 0.335144288960052 1101 44-45 0.23517927536271088 1101 46-47 0.295478823136996 1101 48-49 0.4269784962497738 1101 50-51 0.5036139957888466 1101 52-53 0.46850802218894216 1101 54-55 0.5580784246610833 1101 56-57 0.4795956102829493 1101 58-59 0.4896073102581937 1101 60-61 0.3292865185823004 1101 62-63 0.39532259310343676 1101 64-65 0.31686718875683795 1101 66-67 0.2752784156475343 1101 68-69 0.31961291832104877 1101 70-71 0.3900007458372947 1101 72-73 0.466028307704871 1101 74-75 0.4307392593696058 1101 76-77 0.3494668778120804 1101 78-79 0.38409327237226876 1101 80-81 0.577587254352224 1101 82-83 0.49855104121403215 1101 84-85 0.44808625171982186 1101 86-87 0.6222056108626397 1101 88-89 0.7246158992180227 1101 90-91 0.6961561103182703 1101 92-93 0.737168169842743 1101 94-95 0.8797424470849933 1101 96-97 0.9138696469040504 1101 98-99 1.0338048878237345 1101 100-101 0.9982037953775844 1102 1 0.36059815036205123 1102 2 0.251741031398538 1102 3 0.24889912387201463 1102 4 0.17347983560089375 1102 5 0.09843189248194051 1102 6 0.21912488048651113 1102 7 0.10036159423956548 1102 8 0.2404124488773718 1102 9 0.38641318975532357 1102 10-11 0.377237918357487 1102 12-13 0.22929658179165102 1102 14-15 0.2420036386657003 1102 16-17 0.2660876014545366 1102 18-19 0.2865212426238557 1102 20-21 0.2599568706371542 1102 22-23 0.3198254138372221 1102 24-25 0.29896849020177996 1102 26-27 0.2577010015092398 1102 28-29 0.3295917512786488 1102 30-31 0.35737551257989253 1102 32-33 0.38867319382529786 1102 34-35 0.3075364782528638 1102 36-37 0.4318938941605026 1102 38-39 0.4256171073684456 1102 40-41 0.41907412131974553 1102 42-43 0.4884199356704215 1102 44-45 0.3330711477735804 1102 46-47 0.3400779500645328 1102 48-49 0.28395243573253026 1102 50-51 0.35325631885380915 1102 52-53 0.33545602163250976 1102 54-55 0.34063870094081494 1102 56-57 0.26971350280329176 1102 58-59 0.39545620844307194 1102 60-61 0.27423090202870526 1102 62-63 0.3577141421694563 1102 64-65 0.2819802062638246 1102 66-67 0.3240973789733559 1102 68-69 0.297018576979859 1102 70-71 0.2951459239419201 1102 72-73 0.4002553210336295 1102 74-75 0.3272324790644099 1102 76-77 0.3173882031698483 1102 78-79 0.4991191606597347 1102 80-81 0.6089382478613139 1102 82-83 0.4764838947880854 1102 84-85 0.4301429422254053 1102 86-87 0.4631858839296292 1102 88-89 0.39755736014049603 1102 90-91 0.5933008843453571 1102 92-93 0.7019325359171198 1102 94-95 0.6791999233061041 1102 96-97 0.5993141197774428 1102 98-99 0.4959164082565586 1102 100-101 0.35762269378602696 1103 1 0.3394666483388633 1103 2 0.35259313190626784 1103 3 0.2738006827384041 1103 4 0.19639321209657368 1103 5 0.25902264488195215 1103 6 0.22658892424647092 1103 7 0.20456468256124793 1103 8 0.31509336584655756 1103 9 0.2845183054872109 1103 10-11 0.3159412507287058 1103 12-13 0.27563695345093464 1103 14-15 0.38663906620997324 1103 16-17 0.4282614492004839 1103 18-19 0.40926818437815626 1103 20-21 0.42966799655810917 1103 22-23 0.46347139456825914 1103 24-25 0.4889931708840791 1103 26-27 0.36568539094345454 1103 28-29 0.5037034442236674 1103 30-31 0.5304227154358969 1103 32-33 0.5064102758220272 1103 34-35 0.5603110750431455 1103 36-37 0.6332449762307633 1103 38-39 0.6823974017766616 1103 40-41 0.7432209354955788 1103 42-43 0.5207357212948054 1103 44-45 0.5703215641048729 1103 46-47 0.659294836571334 1103 48-49 0.6710032613943469 1103 50-51 0.6419157301312381 1103 52-53 0.8106827941008987 1103 54-55 0.7909404482416065 1103 56-57 0.8077348262401003 1103 58-59 0.9548030708812689 1103 60-61 0.7163785007612091 1103 62-63 0.6922040219359573 1103 64-65 0.8091722716816179 1103 66-67 0.8518540044879543 1103 68-69 0.7987607054046677 1103 70-71 0.8927728746759804 1103 72-73 0.9395403047910413 1103 74-75 0.8539171834946124 1103 76-77 0.8253419593033549 1103 78-79 0.8721907195808782 1103 80-81 0.9184628675312361 1103 82-83 0.9520447198319495 1103 84-85 1.0186755768105087 1103 86-87 1.0134611631981691 1103 88-89 1.1074262695597916 1103 90-91 1.2099382768012532 1103 92-93 1.121792330633916 1103 94-95 1.1446375815569745 1103 96-97 1.2784615342994847 1103 98-99 1.295862670426768 1103 100-101 1.0876481781891556 1104 1 0.34530270640235017 1104 2 0.32255240524963824 1104 3 0.25463161581833305 1104 4 0.133908399327197 1104 5 0.22984226409703012 1104 6 0.16349381775989258 1104 7 0.16270817739654575 1104 8 0.2055322544208238 1104 9 0.1909477760334397 1104 10-11 0.2709797654550883 1104 12-13 0.2029637366964252 1104 14-15 0.33491246693917986 1104 16-17 0.2941845062346289 1104 18-19 0.36206523122964285 1104 20-21 0.272451684979373 1104 22-23 0.2450018946888548 1104 24-25 0.365504317293599 1104 26-27 0.4806354248416298 1104 28-29 0.48653343752726386 1104 30-31 0.37483404978258505 1104 32-33 0.3856632658179464 1104 34-35 0.38301812664055745 1104 36-37 0.49761049964763515 1104 38-39 0.43766898790886444 1104 40-41 0.46499480584154895 1104 42-43 0.5260521793042301 1104 44-45 0.495452158456402 1104 46-47 0.5100950758397644 1104 48-49 0.5578382868618448 1104 50-51 0.5406636509620526 1104 52-53 0.5560075696917579 1104 54-55 0.6333965086319111 1104 56-57 0.5388453038777143 1104 58-59 0.644472243919715 1104 60-61 0.6827760236164622 1104 62-63 0.5914681502897849 1104 64-65 0.6445350020050711 1104 66-67 0.6792907871598857 1104 68-69 0.6323573745362694 1104 70-71 0.7715377020721483 1104 72-73 0.798231435556918 1104 74-75 0.6920519283138553 1104 76-77 0.7305154243911325 1104 78-79 0.8259934483338149 1104 80-81 0.8917258328349611 1104 82-83 0.8510089484418621 1104 84-85 0.8400795798523895 1104 86-87 0.8504038178437554 1104 88-89 0.973760320420979 1104 90-91 1.0158471883379754 1104 92-93 1.012324762597384 1104 94-95 1.0790815330540937 1104 96-97 1.0015979808122992 1104 98-99 1.1272610699120449 1104 100-101 0.941524764604516 1105 1 0.41718089925779367 1105 2 0.38741096632062266 1105 3 0.3444266685448696 1105 4 0.23236443574219123 1105 5 0.24822812910592518 1105 6 0.26221674201357104 1105 7 0.2555484419526479 1105 8 0.29660461540331795 1105 9 0.3566183603766362 1105 10-11 0.38391874363505707 1105 12-13 0.24494285201721056 1105 14-15 0.3890945179929801 1105 16-17 0.44324219453088887 1105 18-19 0.4243813825442473 1105 20-21 0.4916059610971786 1105 22-23 0.38075446857045137 1105 24-25 0.5064495785480645 1105 26-27 0.312754089120304 1105 28-29 0.4972057955867726 1105 30-31 0.5289926671873317 1105 32-33 0.3973413941021491 1105 34-35 0.5261222398023264 1105 36-37 0.5968671995786821 1105 38-39 0.5172890871041105 1105 40-41 0.5747341193668376 1105 42-43 0.612112130915655 1105 44-45 0.6160516646059477 1105 46-47 0.5204552066828967 1105 48-49 0.4932622249903744 1105 50-51 0.4619595566667769 1105 52-53 0.43295658165870066 1105 54-55 0.4589226945306706 1105 56-57 0.6502412485111932 1105 58-59 0.5941513474157247 1105 60-61 0.5801283368215522 1105 62-63 0.6083567363256606 1105 64-65 0.5392526489544807 1105 66-67 0.720064257014009 1105 68-69 0.6376003005968798 1105 70-71 0.7193004279884683 1105 72-73 0.7722760534904545 1105 74-75 0.7927165570889834 1105 76-77 0.7380809555693482 1105 78-79 0.6757252152608508 1105 80-81 0.687913787640408 1105 82-83 0.7842886495540533 1105 84-85 0.7549215164848775 1105 86-87 0.8671098007815345 1105 88-89 0.9195053609905877 1105 90-91 0.8684747796577952 1105 92-93 0.8406488697271932 1105 94-95 1.0196667697555384 1105 96-97 1.044048509406334 1105 98-99 1.1104880743775567 1105 100-101 1.0880228540396786 1106 1 0.005050481524882855 1106 2 0.08362075280375336 1106 3 8.967776913593184E-4 1106 4 0.09357155766348768 1106 5 0.029997373023938678 1106 6 0.1266760119236281 1106 7 0.07387497143627542 1106 8 0.055357449642428946 1106 9 0.054301825513611846 1106 10-11 -0.3478962503068388 1106 12-13 -0.117355860446402 1106 14-15 -0.11502172728747695 1106 16-17 0.09515112684599814 1106 18-19 0.1429760021403439 1106 20-21 0.017117255320101776 1106 22-23 -0.060412760335523785 1106 24-25 0.02183333829507461 1106 26-27 -0.02684211664419678 1106 28-29 -0.16454881837854884 1106 30-31 0.029985907746983287 1106 32-33 0.06255585362342941 1106 34-35 -0.1518877724144332 1106 36-37 -0.1374030875404273 1106 38-39 -0.043293873777102476 1106 40-41 0.1050925280704007 1106 42-43 -0.02163881802027845 1106 44-45 0.08028966276589244 1106 46-47 0.271299508291186 1106 48-49 0.17546389461486456 1106 50-51 0.25989150476312517 1106 52-53 0.1882363732195813 1106 54-55 -0.12450542765708406 1106 56-57 -0.06762470140510146 1106 58-59 0.015650150876638236 1106 60-61 -0.26532016423673355 1106 62-63 -0.04937087004314833 1106 64-65 -0.0465258178666943 1106 66-67 -0.08190946688710454 1106 68-69 -0.3857809152477145 1106 70-71 -0.21138860004976223 1106 72-73 -0.003348673140585845 1106 74-75 0.06351773552916029 1106 76-77 0.20612632350511362 1106 78-79 0.28575025208710514 1106 80-81 0.1867266750702079 1106 82-83 0.1916963828163354 1106 84-85 0.21396173377235783 1106 86-87 0.285682299486961 1106 88-89 0.15720595724913977 1106 90-91 0.14573469258192517 1106 92-93 0.17286348510020488 1106 94-95 0.1152601249416314 1106 96-97 0.28246426400521685 1106 98-99 0.23900856385253988 1106 100-101 0.1084419443683231 1107 1 0.27987404057567744 1107 2 0.19373948008701802 1107 3 0.2210033874111943 1107 4 0.18977519423357592 1107 5 0.20531985752067072 1107 6 0.25299259199121593 1107 7 0.09449789344269277 1107 8 0.20709607456806367 1107 9 0.1872052977957921 1107 10-11 0.22575557466090146 1107 12-13 0.16106911060510498 1107 14-15 0.23465129365834514 1107 16-17 0.30198836886726355 1107 18-19 0.3659340685107608 1107 20-21 0.39805687212359686 1107 22-23 0.4451168759483295 1107 24-25 0.45981731638055834 1107 26-27 0.4529826429265498 1107 28-29 0.3977356942044352 1107 30-31 0.48788491504118525 1107 32-33 0.5785071710610126 1107 34-35 0.5583599368397429 1107 36-37 0.4610646823429647 1107 38-39 0.40228915903198725 1107 40-41 0.42775303699623635 1107 42-43 0.5387572411990647 1107 44-45 0.6046863867066321 1107 46-47 0.4910377565019388 1107 48-49 0.43215065767007843 1107 50-51 0.4681779458660884 1107 52-53 0.5203239946169873 1107 54-55 0.5443438591867533 1107 56-57 0.5748045714109864 1107 58-59 0.6073409708613369 1107 60-61 0.4785455040862203 1107 62-63 0.5332561721713915 1107 64-65 0.4444637033091752 1107 66-67 0.5138767967991171 1107 68-69 0.5761638590604505 1107 70-71 0.7004211601182853 1107 72-73 0.7600240398097746 1107 74-75 0.7178214369154965 1107 76-77 0.9093867700985356 1107 78-79 0.6856086291733803 1107 80-81 0.8201571489976338 1107 82-83 0.7844253125084428 1107 84-85 0.7763560968876497 1107 86-87 0.9738530086926396 1107 88-89 0.8090386869712951 1107 90-91 0.9544563073817898 1107 92-93 0.949854633187595 1107 94-95 0.8692166661194669 1107 96-97 0.6511219111990165 1107 98-99 0.6060034465623332 1107 100-101 0.7743869930810483 1108 1 0.37965625805985326 1108 2 0.3084757044386137 1108 3 0.23835659100405593 1108 4 0.2708151330851152 1108 5 0.3104866334477805 1108 6 0.3202376754114269 1108 7 0.27521938810313884 1108 8 0.2610944740754704 1108 9 0.273677041586879 1108 10-11 0.3423962393980773 1108 12-13 0.2717820924843224 1108 14-15 0.39305617903021783 1108 16-17 0.45573933412580914 1108 18-19 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64-65 -0.9133445017228397 2316 66-67 -0.9472948791773206 2316 68-69 -0.9963590037414889 2316 70-71 -1.1166865339398022 2316 72-73 -0.8728722756970662 2316 74-75 -0.9441703835720574 2316 76-77 -0.965551758473385 2316 78-79 -0.8430513353893794 2316 80-81 -0.8046018058972706 2316 82-83 -0.8148344658322983 2316 84-85 -0.901063781210155 2316 86-87 -1.05515296647296 2316 88-89 -1.0655532598346902 2316 90-91 -0.9447446582950754 2316 92-93 -1.0441450700360626 2316 94-95 -1.2122566650761932 2316 96-97 -1.2381637202680515 2316 98-99 -1.2346650678550262 2316 100-101 -1.231223056510757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10460.0 3 2888.0 4 3042.0 5 3303.0 6 3913.0 7 4629.0 8 5716.0 9 7159.0 10 8711.0 11 10834.0 12 13393.0 13 16165.0 14 18330.0 15 22270.0 16 27557.0 17 35606.0 18 32879.0 19 27238.0 20 26818.0 21 27958.0 22 29834.0 23 32643.0 24 36485.0 25 40184.0 26 46128.0 27 53229.0 28 62732.0 29 74437.0 30 88505.0 31 108853.0 32 136864.0 33 178610.0 34 246655.0 35 366071.0 36 551277.0 37 727554.0 38 490569.0 39 52667.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41012099408213 17.725379872058532 14.213505264768452 23.650993869090883 2 15.882497958216781 19.006116397481822 39.72372228442832 25.38766335987307 3 19.605767379283616 22.871370561088398 29.080358035679605 28.442504023948377 4 13.232802486529554 15.704537828275845 35.31849430739672 35.744165377797884 5 14.371221186786032 36.56815220425202 33.531517675264055 15.529108933697886 6 34.08468972063433 34.66611471347538 16.366937158640884 14.882258407249402 7 29.923139992219777 32.11369704957633 19.436424227129613 18.52673873107428 8 27.441172460241052 34.15494247744342 18.73437979899816 19.669505263317372 9 26.699039823445187 16.54862556860533 19.402050587625407 37.350284020324075 10-11 25.87298073332048 25.79301113967485 26.155168012603376 22.17884011440129 12-13 26.710462073188058 23.097196339728917 26.838662236873056 23.353679350209966 14-15 23.292840748694566 24.503265924965266 24.980634739789302 27.22325858655087 16-17 22.963251095794973 27.736718849216242 25.965292686279636 23.33473736870915 18-19 23.185777372273332 25.950976110085644 28.287089435355718 22.57615708228531 20-21 23.401551385886265 25.15072127031412 28.78034762428452 22.667379719515097 22-23 23.27392506695862 24.999504112286612 28.73807045682829 22.988500363926484 24-25 23.11685896075768 25.32601357402473 28.630748500271423 22.92637896494617 26-27 22.60443451790187 25.626991428806388 28.529485784860743 23.239088268431 28-29 22.83484513502903 26.09135552479148 28.358520844955954 22.715278495223533 30-31 22.81678053296176 25.62761003382583 28.28116422575626 23.27444520745615 32-33 22.585619894025083 25.591323639514467 28.34193268877772 23.48112377768273 34-35 23.275535131249615 25.68500505640708 28.18980351164406 22.849656300699245 36-37 23.350274085333012 25.22348210283524 28.2074954218093 23.21874839002245 38-39 22.89365154932747 25.6574969072943 28.11375868784171 23.335092855536523 40-41 23.326868842448487 25.524120924265198 28.229497964938606 22.91951226834771 42-43 23.02634079592535 25.535235916953532 27.993602784300958 23.444820502820164 44-45 22.973319610482147 25.793861204145767 28.201065012581196 23.03175417279089 46-47 23.485863163369267 26.04061859409207 27.483845080765303 22.989673161773364 48-49 23.187832261504262 26.18717737724638 27.323145449297968 23.301844911951388 50-51 23.202278783312348 26.37500013769416 27.36561325124558 23.05710782774791 52-53 23.594313253012047 26.028323580034424 27.288481927710844 23.088881239242685 54-55 23.166192544206297 26.277170246222397 27.39070628182427 23.16593092774704 56-57 23.084981273114288 26.477576136105306 27.24972884879463 23.18771374198577 58-59 23.121690994437923 26.058317255873664 27.23646505541423 23.583526694274184 60-61 22.85318502341554 26.24231817324335 27.497944857461924 23.40655194587918 62-63 22.957317325035454 26.246113811287504 27.74545980255821 23.051109061118837 64-65 22.703212894813475 26.357977987722307 27.553239021226766 23.38557009623745 66-67 22.7288084954641 26.363075626758015 27.431121672768228 23.476994205009657 68-69 23.138045098288938 25.93603904709126 27.72598194278555 23.199933911834254 70-71 23.043119363845264 26.182771369291064 27.19764760903509 23.57646165782858 72-73 23.022576618718112 26.287472086318413 27.151846021136727 23.538105273826744 74-75 22.89512526915487 26.744378988783247 27.080921548266524 23.279574193795362 76-77 23.18699198633901 26.32081658628589 27.11941512845802 23.372776298917085 78-79 23.189267391117582 26.535759208552427 27.13043806297128 23.14453533735871 80-81 23.41743935882224 26.56193593564431 26.84288903385233 23.177735671681123 82-83 23.27278510162396 26.608218174658415 26.924721233738204 23.19427548997942 84-85 23.08056745447356 26.779188085636964 26.878017334909167 23.26222712498031 86-87 23.44672463646805 26.697617057610156 26.65974362536168 23.195914680560108 88-89 23.285951864584923 26.72702093330329 26.487822922135116 23.499204279976666 90-91 23.246588900418452 26.74916900372175 26.5816888554908 23.422553240368995 92-93 23.42402795956714 26.388659189759174 26.755752442709667 23.431560407964014 94-95 23.291155280153266 26.163454532683055 26.928581955212387 23.616808231951286 96-97 23.62736844453705 25.83953485713829 26.90939153338962 23.623705164935032 98-99 23.347385577533743 26.276847479491728 26.51228017606295 23.863486766911578 100-101 23.842106917822726 25.564116607539976 26.817414906103288 23.77636156853401 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1574.0 1 1070.0 2 829.0 3 1088.0 4 891.5 5 839.0 6 1646.0 7 3579.0 8 5968.0 9 6908.5 10 5623.5 11 4212.5 12 3896.5 13 3928.0 14 4111.0 15 4284.5 16 4472.5 17 4666.0 18 4866.5 19 5262.5 20 5886.5 21 6944.0 22 8306.0 23 10076.0 24 12472.5 25 15400.0 26 18787.5 27 22677.5 28 25814.0 29 28742.0 30 32620.5 31 39279.5 32 49356.0 33 52921.0 34 51990.0 35 54749.0 36 60014.0 37 66676.0 38 73418.5 39 78887.0 40 84757.0 41 93467.5 42 99095.0 43 102845.0 44 109078.5 45 117852.0 46 123436.0 47 128634.5 48 134705.0 49 135822.5 50 137359.0 51 139722.0 52 142464.5 53 147552.5 54 150590.0 55 143408.5 56 133536.5 57 124919.5 58 116899.5 59 104236.0 60 83889.0 61 68796.5 62 57586.0 63 43483.5 64 31427.5 65 24144.5 66 18053.0 67 13614.5 68 10328.5 69 7625.5 70 5672.5 71 3872.5 72 2665.0 73 1993.5 74 1479.0 75 1068.5 76 778.0 77 481.5 78 287.0 79 190.5 80 119.5 81 69.0 82 55.5 83 43.5 84 24.5 85 16.0 86 13.5 87 12.0 88 7.5 89 6.5 90 7.0 91 8.0 92 5.5 93 3.5 94 5.5 95 3.0 96 0.5 97 0.5 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4916898117047449 2 0.0485385037255986 3 0.05580688998967008 4 0.06525028577973266 5 0.07948420888020598 6 0.10574951378900976 7 0.06750789060417911 8 0.05101636267925933 9 0.049391988476303966 10-11 0.07333085914528183 12-13 0.10620378793051422 14-15 0.05385213459289328 16-17 0.059330956057098684 18-19 0.05840864189101385 20-21 0.07443212979135326 22-23 0.06370850687523265 24-25 0.08749595283037569 26-27 0.06589728228429963 28-29 0.06588351640122374 30-31 0.0700821107393711 32-33 0.06980679307785323 34-35 0.0719818026038443 36-37 0.0677006129672416 38-39 0.03985223150471014 40-41 0.04308721402754498 42-43 0.04201347514762533 44-45 0.020800249327674273 46-47 0.02749046850255825 48-49 0.027311512022571642 50-51 0.025659606053464488 52-53 0.025274161327339485 54-55 0.02472352600430377 56-57 0.024544569524317158 58-59 0.021750095259910885 60-61 0.028440314434794865 62-63 0.0197678080969823 64-65 0.02162620231222785 66-67 0.016051019666491195 68-69 0.018047072712495678 70-71 0.04071948213849139 72-73 0.03667231251417886 74-75 0.031014534569986853 76-77 0.02220436940141535 78-79 0.014977280786571547 80-81 0.016546591457223342 82-83 0.02107556698919213 84-85 0.017950711530964426 86-87 0.009209375777772394 88-89 0.04113245863076818 90-91 0.05195244272842005 92-93 0.03332720292673687 94-95 0.05294358630988435 96-97 0.04244021752297801 98-99 0.0328729287852324 100-101 0.1667323758152156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3632168.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.12087175819693 #Duplication Level Percentage of deduplicated Percentage of total 1 71.48749845146428 32.97065748394286 2 14.405967858893776 13.288315923454933 3 5.470434662301192 7.569036465647656 4 2.653786797058346 4.8957984216289665 5 1.4833482222274679 3.4206656565051223 6 0.9642296539548981 2.6682667329302676 7 0.6560415843678383 2.118004684647138 8 0.48762559727851784 1.799177411047736 9 0.3674635417272125 1.5252964995432252 >10 1.823703044427663 15.111750620901738 >50 0.12724423380638236 4.046336472113097 >100 0.06566902623002172 5.4564270237328465 >500 0.003344361295345171 1.0510960779451346 >1k 0.0034638027555804803 3.0648177800781866 >5k 5.9720737165180695E-5 0.22387539355695107 >10k+ 1.1944147433036139E-4 0.7904773523241805 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16220 0.44656524698196776 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12479 0.34356890980813665 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8128 0.22377819528171605 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4644 0.12785752200889386 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4576 0.1259853619105724 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4207 0.11582614020056341 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3964 0.10913592102567943 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.506353230357186E-5 2 0.0 0.0 0.0 0.0 5.506353230357186E-5 3 0.0 0.0 0.0 2.753176615178593E-5 5.506353230357186E-5 4 0.0 0.0 0.0 8.259529845535779E-5 5.506353230357186E-5 5 0.0 0.0 0.0 8.259529845535779E-5 5.506353230357186E-5 6 0.0 0.0 0.0 8.259529845535779E-5 8.259529845535779E-5 7 0.0 0.0 0.0 8.259529845535779E-5 1.1012706460714372E-4 8 0.0 0.0 0.0 8.259529845535779E-5 1.1012706460714372E-4 9 0.0 0.0 0.0 8.259529845535779E-5 1.1012706460714372E-4 10-11 0.0 0.0 0.0 8.259529845535779E-5 1.1012706460714372E-4 12-13 2.753176615178593E-5 0.0 0.0 8.259529845535779E-5 5.644012061116116E-4 14-15 2.753176615178593E-5 0.0 0.0 1.2389294768303669E-4 7.158259199464341E-4 16-17 2.753176615178593E-5 0.0 0.0 1.7895647998660854E-4 7.708894522500061E-4 18-19 2.753176615178593E-5 0.0 0.0 2.4778589536607337E-4 8.259529845535779E-4 20-21 2.753176615178593E-5 0.0 0.0 3.166153107455382E-4 9.085482830089356E-4 22-23 2.753176615178593E-5 0.0 0.0 4.1297649227678895E-4 0.0010324412306919723 24-25 2.753176615178593E-5 0.0 0.0 7.158259199464341E-4 0.0010462071137678653 26-27 2.753176615178593E-5 0.0 0.0 0.0015142471383482261 0.0010599729968437583 28-29 2.753176615178593E-5 0.0 0.0 0.004336253168906284 0.0011012706460714371 30-31 2.753176615178593E-5 0.0 0.0 0.012458124183683132 0.0011012706460714371 32-33 2.753176615178593E-5 0.0 0.0 0.029596648613169876 0.0011012706460714371 34-35 2.753176615178593E-5 0.0 0.0 0.05657777944192009 0.001183865944526795 36-37 2.753176615178593E-5 0.0 0.0 0.10108287942628205 0.001197631827602688 38-39 2.753176615178593E-5 0.0 0.0 0.1653557875076263 0.0012389294768303668 40-41 2.753176615178593E-5 0.0 0.0 0.23104658154578753 0.0012389294768303668 42-43 2.753176615178593E-5 0.0 0.0 0.3019133476204845 0.0012664612429821528 44-45 2.753176615178593E-5 0.0 0.0 0.38749584270331106 0.0012939930091339386 46-47 2.753176615178593E-5 0.0 0.0 0.48638994672052616 0.0013765883075892967 48-49 2.753176615178593E-5 0.0 0.0 0.589675367438951 0.0015004812552723333 50-51 2.753176615178593E-5 0.0 0.0 0.6984671413877332 0.0015417789045000122 52-53 2.753176615178593E-5 0.0 0.0 0.7998666361247608 0.0017069695014107276 54-55 2.753176615178593E-5 0.0 0.0 0.9090851524488955 0.0017757989167901927 56-57 2.753176615178593E-5 0.0 0.0 1.03633697560245 0.0018583942152455503 58-59 2.753176615178593E-5 0.0 0.0 1.1723301345091968 0.0018996918644732291 60-61 8.259529845535779E-5 0.0 0.0 1.3122741018587245 0.001954755396776801 62-63 8.259529845535779E-5 0.0 0.0 1.4542003563711812 0.001982287162928587 64-65 8.259529845535779E-5 0.0 0.0 1.6011098605571108 0.0021199459936875166 66-67 8.259529845535779E-5 0.0 0.0 1.7711873459597685 0.0021199459936875166 68-69 8.259529845535779E-5 0.0 0.0 1.9574397439766003 0.0021199459936875166 70-71 9.636118153125075E-5 0.0 0.0 2.1470234856977983 0.0022163071752187673 72-73 1.1012706460714372E-4 0.0 0.0 2.3397871464095275 0.002409029538281269 74-75 1.1012706460714372E-4 0.0 0.0 2.538125989766993 0.0025742201351919847 76-77 1.1012706460714372E-4 0.0 0.0 2.751249391547968 0.0026155177844196633 78-79 1.1012706460714372E-4 0.0 0.0 2.985228106188921 0.0026155177844196633 80-81 1.1012706460714372E-4 0.0 1.3765883075892964E-5 3.2255115952786326 0.002684347199799128 82-83 1.2389294768303669E-4 0.0 2.753176615178593E-5 3.474178507161563 0.0028908354459375226 84-85 1.3765883075892964E-4 0.0 2.753176615178593E-5 3.7316693500961406 0.0029321330951652016 86-87 1.3765883075892964E-4 0.0 2.753176615178593E-5 3.9961945592825 0.0031248554582277033 88-89 1.3765883075892964E-4 2.753176615178593E-5 2.753176615178593E-5 4.2809143189411945 0.0033175778212902046 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7185 0.0 53.82892 1 GTATCAA 12390 0.0 40.0407 1 TATCAAC 15440 0.0 31.794952 2 ATCAACG 15660 0.0 31.166199 3 TCAACGC 15750 0.0 30.988108 4 CAACGCA 16040 0.0 30.39822 5 AACGCAG 16620 0.0 29.365988 6 TGGTATC 3305 0.0 26.745249 2 TAAGGTG 2305 0.0 25.77177 5 GTGGTAT 3655 0.0 25.605078 1 ACGCAGA 19025 0.0 25.515837 7 CGCAGAG 19125 0.0 25.332352 8 GTGTAAG 1400 0.0 23.874123 1 AGGTAAG 2115 0.0 23.143658 2 GTACATG 12375 0.0 22.533314 1 GAGTACT 12930 0.0 22.389463 12-13 GTAAGGT 2360 0.0 22.35199 4 GCAGAGT 21545 0.0 22.132946 9 TACATGG 12390 0.0 22.13144 2 AAGGTAA 2185 0.0 21.852745 1 >>END_MODULE