FastQCFastQC Report
Thu 26 May 2016
SRR938173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938173_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3632168
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157790.4344237381090302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128920.3549395292288242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84490.2326158922164393No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA47340.1303353809625546No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA45900.12637080663669742No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA43280.1191574839049295No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA40820.11238466943159016No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC39610.10905332572722407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71100.050.8493041
GTATCAA125900.041.433021
ATCAACG157300.032.8241163
TCAACGC157800.032.7174074
TATCAAC158600.032.6807142
CAACGCA161550.031.9873525
AACGCAG168200.030.750936
ACGCAGA191650.026.7400997
TAAGGTG22150.026.3747125
CGCAGAG194250.026.3332868
GTAAGGT20450.024.6189144
AGGTAAG19800.024.4739992
GTACATG122850.023.2417741
GCAGAGT218300.022.888249
TGGTATC34550.022.6885032
TACATGG123600.022.5626222
GAGTACT131950.021.884912-13
GTGGTAT38050.021.600381
ACATGGG121150.021.4464863
AGAGTAC185900.021.38436910-11
Produced by FastQC (version 0.11.5)