FastQCFastQC Report
Thu 26 May 2016
SRR938172_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938172_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3615690
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152930.42296214553791944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121720.33664390475953415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77730.2149797134156966No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA46990.1299613628380752No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA46700.12915930292696554No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA40930.11320107641971519No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40080.11085021116301454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70300.054.1263961
GTATCAA126050.043.0326921
TATCAAC159200.033.63462
ATCAACG160650.033.1839373
TCAACGC163400.032.6254544
CAACGCA167200.031.9408515
AACGCAG172950.030.8742166
ACGCAGA195950.026.905737
CGCAGAG197150.026.7148888
GTGGTAT32750.025.156631
TGGTATC31650.024.941662
GCAGAGT220950.023.3199489
GTACATG128000.023.067441
GAGTACT133750.022.62022412-13
TAAGGTG21500.022.561335
TACATGG129100.022.4327262
GTACTTT140300.021.36031714-15
AGAGTAC192600.021.30999210-11
CAGAGTA206350.021.20296110-11
ACATGGG126800.021.1150513
Produced by FastQC (version 0.11.5)