##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938172_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3615690 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.964050845066918 33.0 31.0 34.0 30.0 34.0 2 32.078785791923536 34.0 31.0 34.0 30.0 34.0 3 32.10635978195033 34.0 31.0 34.0 30.0 34.0 4 35.61686317134489 37.0 35.0 37.0 33.0 37.0 5 35.61668948388827 37.0 35.0 37.0 33.0 37.0 6 35.647507114824556 37.0 35.0 37.0 33.0 37.0 7 35.575502877735644 37.0 35.0 37.0 33.0 37.0 8 35.56447538367504 37.0 35.0 37.0 33.0 37.0 9 37.20178195586458 39.0 37.0 39.0 34.0 39.0 10-11 37.11178806811425 39.0 37.0 39.0 34.0 39.0 12-13 36.96400562548227 39.0 37.0 39.0 33.0 39.0 14-15 38.15949182590322 40.0 38.0 41.0 33.0 41.0 16-17 37.98898813227903 40.0 38.0 41.0 33.0 41.0 18-19 38.14114512029516 40.0 38.0 41.0 33.0 41.0 20-21 38.128282845044794 40.0 38.0 41.0 33.0 41.0 22-23 38.02056025267653 40.0 38.0 41.0 33.0 41.0 24-25 37.90085087493674 40.0 38.0 41.0 33.0 41.0 26-27 37.72038753322326 40.0 38.0 41.0 32.0 41.0 28-29 37.54409020131703 40.0 37.5 41.0 32.0 41.0 30-31 37.32527318437145 40.0 37.0 41.0 31.5 41.0 32-33 37.04224297437004 40.0 36.5 41.0 31.0 41.0 34-35 36.81061222062732 39.5 36.0 41.0 30.0 41.0 36-37 36.552318506287875 39.0 35.5 41.0 30.0 41.0 38-39 36.26092308798597 39.0 35.0 40.5 30.0 41.0 40-41 36.15908000962472 39.0 35.0 41.0 29.5 41.0 42-43 35.952543221349174 39.0 35.0 41.0 28.5 41.0 44-45 35.63744637952922 39.0 35.0 40.0 27.5 41.0 46-47 35.271878811513155 38.5 35.0 40.0 26.0 41.0 48-49 34.866175888972776 38.0 34.0 40.0 24.5 41.0 50-51 34.12069563485808 37.5 33.5 39.5 23.5 40.5 52-53 34.185260074840485 38.0 33.5 40.0 24.0 40.5 54-55 34.2577646313705 38.0 34.0 40.0 23.0 41.0 56-57 33.9281908847274 38.0 33.5 40.0 22.0 41.0 58-59 33.50546908059043 37.0 33.0 40.0 20.5 41.0 60-61 32.98908659204744 37.0 32.5 40.0 17.5 41.0 62-63 32.43723715252138 36.0 31.5 39.0 13.5 41.0 64-65 31.890179191247036 35.5 31.0 39.0 9.0 40.0 66-67 31.328103764426707 35.0 30.5 38.5 6.5 40.0 68-69 30.65604269171306 35.0 29.5 38.0 2.0 40.0 70-71 30.067925624154725 34.5 29.0 37.0 2.0 39.5 72-73 29.39170517937102 34.0 28.5 36.5 2.0 39.0 74-75 28.738965453343624 34.0 27.5 36.0 2.0 39.0 76-77 28.022457539224877 33.5 26.0 35.0 2.0 37.5 78-79 27.289748706332677 33.0 25.5 35.0 2.0 37.0 80-81 26.597454289499375 32.5 24.5 35.0 2.0 36.5 82-83 25.89497620094643 32.0 21.5 35.0 2.0 36.0 84-85 25.205013704161583 31.5 19.0 34.5 2.0 35.5 86-87 24.490532097607925 31.0 15.5 34.0 2.0 35.0 88-89 23.626320702272594 30.5 6.5 34.0 2.0 35.0 90-91 22.754527904770598 30.0 2.0 34.0 2.0 35.0 92-93 21.847306184988206 29.0 2.0 34.0 2.0 35.0 94-95 20.786785094961125 29.0 2.0 34.0 2.0 35.0 96-97 19.534807464135476 26.5 2.0 33.5 2.0 35.0 98-99 17.979194565905814 24.0 2.0 33.0 2.0 35.0 100-101 14.04963022272374 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11803740089708725 1101 2 0.06472543565507749 1101 3 0.041293970662366064 1101 4 -0.10421019808657661 1101 5 0.039772342982722364 1101 6 -0.07135144130901239 1101 7 0.0119021133494428 1101 8 0.056450621579301696 1101 9 -0.08694838069168753 1101 10-11 0.07859421091302465 1101 12-13 0.05389155644335375 1101 14-15 0.14111004508460923 1101 16-17 0.1461634385504027 1101 18-19 0.2689643730901352 1101 20-21 0.21693922067515814 1101 22-23 0.11377011240681867 1101 24-25 0.22716817240090137 1101 26-27 0.1664199686159762 1101 28-29 0.23582819881006856 1101 30-31 0.13737480659113288 1101 32-33 0.08595836614745878 1101 34-35 0.078585109007129 1101 36-37 0.020161639886801197 1101 38-39 0.009932309696978336 1101 40-41 0.14678340318123873 1101 42-43 0.14980400815587558 1101 44-45 0.18211456297193962 1101 46-47 0.27638581381327043 1101 48-49 0.2712815281990899 1101 50-51 0.2582455322571704 1101 52-53 0.3393370300640157 1101 54-55 0.21115166741939362 1101 56-57 0.2516699571563734 1101 58-59 0.14210719371543235 1101 60-61 0.042299987561769115 1101 62-63 -0.16622866013771898 1101 64-65 -0.0938866166863157 1101 66-67 -0.0031718452490352433 1101 68-69 -0.0703735310160134 1101 70-71 -0.07595668444446702 1101 72-73 -5.513739658624672E-4 1101 74-75 -0.15912676993712793 1101 76-77 -0.4028588424842674 1101 78-79 -0.3038900406888345 1101 80-81 -0.531064926831359 1101 82-83 -0.75785754757813 1101 84-85 -0.7264023471433809 1101 86-87 -0.7457163030548948 1101 88-89 -0.7309299723360851 1101 90-91 -0.7112133156744989 1101 92-93 -0.8425483863496659 1101 94-95 -1.0046356613115002 1101 96-97 -1.1221782050991607 1101 98-99 -1.345071603106497 1101 100-101 -1.3797139206711737 1102 1 0.1502603602340855 1102 2 0.18330491515524017 1102 3 0.15963945088113007 1102 4 0.07797891435932769 1102 5 0.07499965007500009 1102 6 0.165689513534943 1102 7 0.12104062590916698 1102 8 0.1199185649466088 1102 9 0.149769543061133 1102 10-11 0.16490970454153597 1102 12-13 0.07845021984115164 1102 14-15 0.15549349345789665 1102 16-17 0.21760882239910728 1102 18-19 0.1474208850691454 1102 20-21 0.16051266779438578 1102 22-23 0.29737358291328775 1102 24-25 0.16951531736646785 1102 26-27 0.15037187917305772 1102 28-29 -0.020389964651911896 1102 30-31 0.0742643650631436 1102 32-33 0.08109502997540119 1102 34-35 0.13625821296265883 1102 36-37 0.08782348903731219 1102 38-39 -0.016637552675916822 1102 40-41 0.02117464025180027 1102 42-43 0.19791042210472654 1102 44-45 0.32429566678451494 1102 46-47 0.26286868089977133 1102 48-49 0.20896502413354057 1102 50-51 0.01769696730826098 1102 52-53 -0.008844014112050047 1102 54-55 0.032631257670047376 1102 56-57 -0.01855781624265518 1102 58-59 -0.05978322018097515 1102 60-61 -0.10097566824422444 1102 62-63 -0.2058916440737164 1102 64-65 -0.29957764204727155 1102 66-67 -0.39782934452948737 1102 68-69 -0.36144415861836876 1102 70-71 -0.6696930640050489 1102 72-73 -0.8562183332461828 1102 74-75 -0.8692146661154645 1102 76-77 -0.9691191279316698 1102 78-79 -1.1139492923098615 1102 80-81 -1.0414029791325738 1102 82-83 -0.9797256104722294 1102 84-85 -0.9163856023958914 1102 86-87 -0.671422377117942 1102 88-89 -0.7729735526667838 1102 90-91 -0.8427396275349537 1102 92-93 -0.8527873616844701 1102 94-95 -1.0193470047465247 1102 96-97 -1.1413089416502267 1102 98-99 -1.0258473620075108 1102 100-101 -1.3130744041244746 1103 1 0.23838723862505162 1103 2 0.1886570966046861 1103 3 0.19860833953385537 1103 4 0.18030870285240752 1103 5 0.18283016033716848 1103 6 0.16688961062958896 1103 7 0.152278977167974 1103 8 0.16786106011804236 1103 9 0.24584212090793045 1103 10-11 0.21160097898528107 1103 12-13 0.2189102379397312 1103 14-15 0.3283696687982669 1103 16-17 0.22656677985296625 1103 18-19 0.2694314104993296 1103 20-21 0.3188147331891642 1103 22-23 0.36547801118603473 1103 24-25 0.24766151404995185 1103 26-27 0.1558874449104053 1103 28-29 0.3208845897875108 1103 30-31 0.28305871682754713 1103 32-33 0.31651603913176274 1103 34-35 0.30564902455637366 1103 36-37 0.08178747867733449 1103 38-39 0.12310839507170357 1103 40-41 0.2045733273002952 1103 42-43 0.36230530748715495 1103 44-45 0.27129127780048634 1103 46-47 0.2826153854381843 1103 48-49 0.3011033465698958 1103 50-51 0.24305562777245626 1103 52-53 0.31421301611208463 1103 54-55 0.21549359943838198 1103 56-57 0.21972661954968942 1103 58-59 0.34499705084674304 1103 60-61 0.33475347150203305 1103 62-63 0.35761839990871636 1103 64-65 0.1994417503141328 1103 66-67 0.010503616446676745 1103 68-69 -0.008775122552460601 1103 70-71 -0.15645964114497346 1103 72-73 -0.11017523199804202 1103 74-75 -0.08733294204943931 1103 76-77 0.05606424287890732 1103 78-79 -0.07830952834807903 1103 80-81 -0.13140361918435772 1103 82-83 -0.42000037584695704 1103 84-85 -0.4052697345058007 1103 86-87 -0.5877248382176887 1103 88-89 -0.26636345812428885 1103 90-91 -0.19096468141604106 1103 92-93 -0.48496520027387646 1103 94-95 -0.7256742889866779 1103 96-97 -0.9152914605881115 1103 98-99 -1.0388091150892222 1103 100-101 -0.9943813496121905 1104 1 0.24070930840844085 1104 2 0.24564337786071633 1104 3 0.22283601240197015 1104 4 0.22457316983346232 1104 5 0.2595270907062428 1104 6 0.2865214554499076 1104 7 0.24822099733676595 1104 8 0.31205000866562216 1104 9 0.31303550142244774 1104 10-11 0.36613127761167874 1104 12-13 0.39082446875199395 1104 14-15 0.43416738905035146 1104 16-17 0.437834338545926 1104 18-19 0.43213049601654063 1104 20-21 0.3879766719737958 1104 22-23 0.4755910034359019 1104 24-25 0.4831664623662064 1104 26-27 0.4010904190073177 1104 28-29 0.32749522222717076 1104 30-31 0.3906446955592173 1104 32-33 0.4259754270381464 1104 34-35 0.46323949083294025 1104 36-37 0.34475366699799537 1104 38-39 0.3734269356545141 1104 40-41 0.44249610472632384 1104 42-43 0.4140226665125226 1104 44-45 0.40876454029444886 1104 46-47 0.3611323062989271 1104 48-49 0.23479409689491604 1104 50-51 0.12074087888345275 1104 52-53 0.30534086758073187 1104 54-55 0.28130659048657947 1104 56-57 0.3397370892360385 1104 58-59 0.2728494864758204 1104 60-61 0.2690141306416436 1104 62-63 0.23652115574947175 1104 64-65 0.003544079856904858 1104 66-67 0.12588191648205438 1104 68-69 0.32062334740126985 1104 70-71 0.2909552982863133 1104 72-73 0.2795637712637884 1104 74-75 0.10164405167851598 1104 76-77 0.06648403111185175 1104 78-79 -0.051785837866255235 1104 80-81 -0.08305783338332517 1104 82-83 -0.2106291334617012 1104 84-85 -0.09769969688552749 1104 86-87 -0.08163351236034089 1104 88-89 -0.029833650311381632 1104 90-91 -0.28299028595781195 1104 92-93 -0.37125203287831354 1104 94-95 -0.4498544295538558 1104 96-97 -0.705213429880537 1104 98-99 -0.7098212836586839 1104 100-101 -0.9373557319269814 1105 1 0.06916705940670198 1105 2 0.138010613808369 1105 3 0.13444125127957562 1105 4 0.07275588810090738 1105 5 0.15137972450280301 1105 6 0.0919218838881406 1105 7 0.11852143947222515 1105 8 0.23213136464967477 1105 9 0.269432855284478 1105 10-11 0.21726224642301162 1105 12-13 0.27788598356830363 1105 14-15 0.19645817602922477 1105 16-17 0.21132348018849 1105 18-19 0.31177334421994374 1105 20-21 0.30382474952931204 1105 22-23 0.29227404709947535 1105 24-25 0.28359095482125696 1105 26-27 0.2832407033465145 1105 28-29 0.23241006821676535 1105 30-31 0.1551348613652337 1105 32-33 0.2544532545601257 1105 34-35 0.08077928059089601 1105 36-37 0.13249283836425008 1105 38-39 0.2391689205152474 1105 40-41 0.08568234985668255 1105 42-43 0.21276672785943163 1105 44-45 0.19149201165367913 1105 46-47 0.09819624594210552 1105 48-49 -0.0011041007025767158 1105 50-51 -0.038349014924769165 1105 52-53 0.120625072936285 1105 54-55 0.09030497532589976 1105 56-57 0.15927895165604866 1105 58-59 0.14621085973089976 1105 60-61 0.18772915316558425 1105 62-63 0.4051342312504076 1105 64-65 0.1412463052048416 1105 66-67 0.07054932235879008 1105 68-69 0.1916926943215138 1105 70-71 -0.0071285843847448405 1105 72-73 -0.06269719816636155 1105 74-75 -0.06610273486093021 1105 76-77 -0.19862446555109514 1105 78-79 -0.4727141884903112 1105 80-81 -0.3853080292815285 1105 82-83 -0.5556992518071588 1105 84-85 -0.460862239550309 1105 86-87 -0.4401437334836942 1105 88-89 -0.3692381041360733 1105 90-91 -0.16968577358963088 1105 92-93 -0.34407897950152844 1105 94-95 -0.5245468328754015 1105 96-97 -0.7884277525942309 1105 98-99 -0.8762569254503916 1105 100-101 -1.105104430516306 1106 1 0.24334633270514416 1106 2 0.24468370248207805 1106 3 0.22768466201766557 1106 4 0.23362263435407726 1106 5 0.2020966372135149 1106 6 0.1302025908307911 1106 7 0.2229776310190985 1106 8 0.107663119155049 1106 9 0.3245029247450759 1106 10-11 0.2731151791999693 1106 12-13 0.312365085226638 1106 14-15 0.32019964204441465 1106 16-17 -0.19368766953197536 1106 18-19 0.21054106725603106 1106 20-21 0.408850477423222 1106 22-23 0.388671244973402 1106 24-25 0.3236936163300186 1106 26-27 0.23516263029654283 1106 28-29 0.22102255771577006 1106 30-31 0.15140401775559553 1106 32-33 0.23581258180052345 1106 34-35 0.26782983976215036 1106 36-37 0.27569598471280443 1106 38-39 0.28525021870294864 1106 40-41 0.348002718491486 1106 42-43 0.010852049224347127 1106 44-45 0.16215993843990617 1106 46-47 0.2316670798100091 1106 48-49 0.31683988214501113 1106 50-51 0.19760651412611452 1106 52-53 0.2663466010115414 1106 54-55 0.1942729928610163 1106 56-57 0.048992849792583115 1106 58-59 0.19914756889443197 1106 60-61 0.40187182972184843 1106 62-63 0.2017448829391526 1106 64-65 0.11145115526982252 1106 66-67 0.04737605175237647 1106 68-69 -0.0617481713882313 1106 70-71 -0.18351107361555918 1106 72-73 -0.17409161268734152 1106 74-75 -0.07624471304205471 1106 76-77 -0.4958140763963179 1106 78-79 -0.41545290811896507 1106 80-81 -0.547885604372258 1106 82-83 -0.4116472824762276 1106 84-85 -0.45877187082128046 1106 86-87 -0.5731508564027941 1106 88-89 -0.9334821393164958 1106 90-91 -1.025514886591587 1106 92-93 -0.8209924168626799 1106 94-95 -1.0986596753673439 1106 96-97 -0.9666842936548434 1106 98-99 -1.0820477764098158 1106 100-101 -1.1884313762720922 1107 1 0.03885933080518811 1107 2 0.07142809643129766 1107 3 0.09407026478724134 1107 4 0.11399512015351831 1107 5 0.06986496432827494 1107 6 0.08656891896331587 1107 7 0.16090313189441474 1107 8 0.11333007857614064 1107 9 0.13167305043300104 1107 10-11 0.2126620041898022 1107 12-13 0.1889473590443771 1107 14-15 0.13888948248342814 1107 16-17 0.19435377879687366 1107 18-19 0.25753748543510113 1107 20-21 0.30536424375804216 1107 22-23 0.4515402579980261 1107 24-25 0.32551633191221896 1107 26-27 0.2587921122390924 1107 28-29 0.2413126252850617 1107 30-31 0.20354684753166907 1107 32-33 0.32616772337381406 1107 34-35 0.38384471031164225 1107 36-37 0.38047149217018017 1107 38-39 0.2557798929989161 1107 40-41 0.2968982827707265 1107 42-43 0.30090672455182244 1107 44-45 0.2599776162882179 1107 46-47 0.19410302749930963 1107 48-49 0.24830034850870675 1107 50-51 0.2419739140541992 1107 52-53 0.1410087791044745 1107 54-55 0.012632870572190313 1107 56-57 0.028958819546531345 1107 58-59 0.049814322012068146 1107 60-61 0.23691453453697164 1107 62-63 0.27906017257937776 1107 64-65 0.20757923238408793 1107 66-67 0.18913415505735642 1107 68-69 -0.013962028496564471 1107 70-71 -0.026311156726382734 1107 72-73 0.040939583342197494 1107 74-75 -0.026874208738171035 1107 76-77 -0.26167684726783946 1107 78-79 -0.32990451424048217 1107 80-81 -0.356074967366137 1107 82-83 -0.5512847581864513 1107 84-85 -0.6498670769184329 1107 86-87 -0.49478513833906135 1107 88-89 -0.5913071065535433 1107 90-91 -0.6890170056227838 1107 92-93 -0.7568999506718868 1107 94-95 -0.8875500869722082 1107 96-97 -0.7177250209039947 1107 98-99 -0.9789424673377667 1107 100-101 -1.036926785529026 1108 1 -0.011275416849944264 1108 2 0.0026181532416273967 1108 3 0.07900609497754374 1108 4 0.08238837837946988 1108 5 0.09345840861495702 1108 6 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-0.7408680633700655 2316 92-93 -0.9189091940490535 2316 94-95 -0.8002502013232657 2316 96-97 -0.9686898579537377 2316 98-99 -0.8794213123920827 2316 100-101 -0.45199730201463595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12086.0 3 3576.0 4 3380.0 5 3809.0 6 4451.0 7 5603.0 8 7056.0 9 8669.0 10 10903.0 11 13515.0 12 16991.0 13 20651.0 14 23484.0 15 28531.0 16 34355.0 17 42601.0 18 42237.0 19 37473.0 20 38544.0 21 41109.0 22 44659.0 23 49612.0 24 55193.0 25 61898.0 26 69977.0 27 79643.0 28 91184.0 29 107160.0 30 125849.0 31 150707.0 32 183134.0 33 229898.0 34 297840.0 35 396061.0 36 501357.0 37 516238.0 38 242690.0 39 13565.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.40861188966823 17.679695738123392 14.24364826188104 23.668044110327337 2 15.880754668112226 19.023876903902707 39.68690255510295 25.40846587288213 3 19.608919606759066 22.869372478674336 29.10192886606962 28.419779048496984 4 13.260122621572679 15.683032733518381 35.34493859920957 35.71190604569937 5 14.392807254152205 36.48576638927584 33.57954991025971 15.541876446312253 6 34.11281593099775 34.66379859341736 16.3225218943694 14.900863581215487 7 29.884370628992862 32.12451481734194 19.42259835449978 18.568516199165423 8 27.43849988541898 34.17370481937714 18.757742521513002 19.63005277369087 9 26.741769544761144 16.509590833029428 19.381261053805083 37.36737856840435 10-11 25.87534905920645 25.763126861747153 26.184399605706542 22.177124473339855 12-13 26.730386848526745 23.08514334963478 26.78826434406959 23.396205457768886 14-15 23.30608512789707 24.485774420530777 24.96313384412121 27.245006607450943 16-17 23.003397155960243 27.718898326125586 25.948522140918623 23.32918237699555 18-19 23.171854149614802 25.984018752010396 28.239843064903553 22.604284033471252 20-21 23.385916974408108 25.156940172405367 28.790793194013432 22.666349659173097 22-23 23.244203923463857 25.002798832138794 28.74801033517262 23.00498690922473 24-25 23.087924261703773 25.33032816214399 28.616079310355342 22.965668265796896 26-27 22.597090978688033 25.596199523624808 28.514250332270247 23.29245916541692 28-29 22.86740447539717 26.071941820473743 28.3312127874662 22.729440916662895 30-31 22.823077635417057 25.6035573308004 28.275278978856477 23.298086054926067 32-33 22.623781068061916 25.577712367161702 28.333593403530593 23.464913161245786 34-35 23.284141861233152 25.68060322001181 28.16051447992123 22.87474043883381 36-37 23.345584495302184 25.209500405873086 28.21262905414617 23.232286044678567 38-39 22.905983449952565 25.676910609848036 28.08813594122463 23.32896999897477 40-41 23.33395183722638 25.51931842254664 28.224781749070893 22.921947991156085 42-43 23.004904473193935 25.52631345899028 27.962429665282574 23.506352402533214 44-45 22.965363767185238 25.80883958119883 28.16375921671641 23.062037434899526 46-47 23.470043539601196 26.059258629990595 27.422726512392025 23.047971318016184 48-49 23.214644423010196 26.15221985560106 27.25415131729306 23.378984404095675 50-51 23.22761870343139 26.38267574383868 27.30664566542161 23.083059887308323 52-53 23.636555026359005 25.99503306494806 27.246034530074937 23.122377378618 54-55 23.1786359624 26.23023219133163 27.34681764222445 23.24431420404391 56-57 23.142671551892988 26.486191969033186 27.160257481378856 23.210878997694973 58-59 23.20775178225189 26.05265830378709 27.14483842198312 23.594751491977895 60-61 22.85868946909432 26.29958288512021 27.408357016693447 23.43337062909202 62-63 22.9825634776083 26.222468685265405 27.62537100897284 23.16959682815346 64-65 22.736354266820687 26.34855328597417 27.48906058698615 23.426031860218995 66-67 22.768204538094203 26.310025771452356 27.405613387265838 23.5161563031876 68-69 23.210193476627726 25.918387482130406 27.651649911349104 23.219769129892768 70-71 23.110279296959312 26.155338940745832 27.098047810220187 23.636333952074665 72-73 23.054837179726288 26.291028821797845 27.048694720495693 23.605439277980178 74-75 22.924700229058992 26.70599281792086 27.012788262307335 23.35651869071281 76-77 23.212636543761434 26.340125798342285 26.99922888866605 23.44800876923024 78-79 23.299021661597074 26.466369937260254 26.986252693228092 23.24835570791458 80-81 23.500213638180053 26.494523213389336 26.691714919110044 23.313548229320563 82-83 23.390491898014474 26.54451987483421 26.78232823695246 23.28265999019886 84-85 23.21921537965097 26.68333536885693 26.74352969750552 23.353919553986582 86-87 23.56054254901101 26.6031375511764 26.48649291646305 23.34982698334954 88-89 23.418535917385675 26.644168730645656 26.29107714864952 23.646218203319147 90-91 23.34644053830909 26.640463331666552 26.392550544693798 23.620545585330564 92-93 23.597010868512207 26.254836247122054 26.53627828882796 23.61187459553778 94-95 23.381032422667385 26.07318799323664 26.689933592527197 23.85584599156877 96-97 23.761636940196688 25.698852952914226 26.644199532944878 23.895310573944215 98-99 23.456664400736702 26.096761066886348 26.24099099130262 24.205583541074326 100-101 24.504789270969606 24.861519894278242 26.327296019815076 24.306394814937075 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1480.0 1 1015.5 2 770.5 3 1004.0 4 864.0 5 820.0 6 1526.0 7 3344.5 8 5536.0 9 6380.0 10 5245.5 11 3973.0 12 3725.0 13 3803.5 14 3988.5 15 4217.5 16 4415.0 17 4663.0 18 5059.0 19 5451.0 20 5965.5 21 6979.0 22 8476.0 23 10270.0 24 12505.5 25 15578.0 26 18785.5 27 22508.0 28 25833.0 29 28807.0 30 32847.5 31 39539.0 32 49160.5 33 52830.0 34 52033.0 35 54524.5 36 59685.5 37 65941.5 38 72730.5 39 78240.5 40 83961.5 41 92278.5 42 97525.0 43 101347.0 44 107490.5 45 116024.5 46 121969.5 47 127018.5 48 132761.5 49 134362.0 50 135778.0 51 137620.5 52 140460.5 53 145494.0 54 148253.0 55 142350.5 56 133122.5 57 124860.0 58 116868.5 59 104553.0 60 85326.0 61 69917.5 62 58774.5 63 45214.0 64 33213.0 65 25589.5 66 19162.5 67 14281.0 68 10859.0 69 8245.5 70 6286.0 71 4401.5 72 3002.5 73 2207.5 74 1654.0 75 1222.5 76 872.5 77 577.0 78 365.5 79 226.0 80 129.0 81 75.5 82 46.5 83 36.0 84 30.0 85 20.0 86 15.0 87 12.5 88 10.0 89 7.5 90 6.5 91 4.5 92 2.5 93 5.0 94 4.5 95 1.5 96 1.5 97 2.0 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14876828489168042 2 0.05152543497921559 3 0.15172760939129185 4 0.1311782813238967 5 0.19202420561497252 6 0.19990651853449823 7 0.16503074102038615 8 0.06986218398148072 9 0.1210557321009268 10-11 0.10121166361054183 12-13 0.15152018010393592 14-15 0.1381064195215851 16-17 0.1348428654005183 18-19 0.1319388553775351 20-21 0.13384720482120976 22-23 0.19476227220807094 24-25 0.2126011909206818 26-27 0.14659719168402158 28-29 0.1269882097193067 30-31 0.09800342396610329 32-33 0.1240565424580094 34-35 0.09245814768412115 36-37 0.08594486806114462 38-39 0.051539263598372645 40-41 0.05895140346655826 42-43 0.06233941516003861 44-45 0.040628483083450184 46-47 0.051262691215231394 48-49 0.04248151805049658 50-51 0.05354441337614673 52-53 0.027270036977727626 54-55 0.051442463264273215 56-57 0.027159408024471126 58-59 0.05152543497921559 60-61 0.03700538486429976 62-63 0.05243812384358172 64-65 0.04051785413019368 66-67 0.03925944978690098 68-69 0.06525725380217884 70-71 0.05260406727346647 72-73 0.05055743163822119 74-75 0.04302083419762203 76-77 0.021503502789232486 78-79 0.023135279849765884 80-81 0.025818031966236044 82-83 0.02225024822371387 84-85 0.02023126982678272 86-87 0.02390968252256139 88-89 0.06420627874624207 90-91 0.10885889000439751 92-93 0.0793209594849116 94-95 0.07448094277993965 96-97 0.06670925881367042 98-99 0.05574316382211971 100-101 0.13004433455301756 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3615690.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.547932466074876 #Duplication Level Percentage of deduplicated Percentage of total 1 71.97964541629396 33.50503673769668 2 13.969787081422528 13.00529411263002 3 5.5246733876722205 7.714863712394628 4 2.6729126486450103 4.976742298273811 5 1.4836719489614802 3.453093084103434 6 0.940191585231531 2.625838464871751 7 0.6415056559334443 2.0902533364296523 8 0.4645187728344179 1.7297910773696368 9 0.3534078457471057 1.4805364083135557 >10 1.771652466541556 14.870952712309302 >50 0.12638335681176338 4.045033746792026 >100 0.06474859536900081 5.439348889907518 >500 0.0034506192751458083 1.1103494028184737 >1k 0.0032721389543584065 2.9770213438209128 >5k 5.9493435533789204E-5 0.21525741068008628 >10k+ 1.1898687106757841E-4 0.7605872615886492 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15293 0.42296214553791944 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12172 0.33664390475953415 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7773 0.2149797134156966 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4699 0.1299613628380752 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4670 0.12915930292696554 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4093 0.11320107641971519 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4008 0.11085021116301454 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1062895325650152E-4 2 0.0 0.0 0.0 0.0 1.382861915706269E-4 3 0.0 0.0 0.0 0.0 1.382861915706269E-4 4 0.0 0.0 0.0 0.0 1.382861915706269E-4 5 0.0 0.0 0.0 0.0 1.382861915706269E-4 6 0.0 0.0 0.0 0.0 1.382861915706269E-4 7 0.0 0.0 0.0 0.0 1.382861915706269E-4 8 0.0 0.0 0.0 0.0 1.382861915706269E-4 9 0.0 0.0 0.0 0.0 1.659434298847523E-4 10-11 0.0 0.0 0.0 1.382861915706269E-5 1.659434298847523E-4 12-13 0.0 0.0 0.0 4.1485857471188075E-5 4.978302896542568E-4 14-15 0.0 0.0 0.0 9.680033409943884E-5 5.669733854395704E-4 16-17 1.382861915706269E-5 0.0 0.0 1.1062895325650152E-4 6.22287862067821E-4 18-19 2.765723831412538E-5 0.0 0.0 1.659434298847523E-4 6.914309578531345E-4 20-21 2.765723831412538E-5 0.0 0.0 2.3508652567006574E-4 8.297171494237613E-4 22-23 2.765723831412538E-5 0.0 0.0 3.5954409808363E-4 9.126888643661376E-4 24-25 2.765723831412538E-5 0.0 0.0 5.531447662825076E-4 9.403461026802629E-4 26-27 2.765723831412538E-5 0.0 0.0 0.0011477753900362032 0.0010094891984655764 28-29 2.765723831412538E-5 0.0 0.0 0.004051785413019368 0.0011062895325650154 30-31 2.765723831412538E-5 0.0 0.0 0.013745647442120314 0.0011754326283503287 32-33 4.1485857471188075E-5 0.0 0.0 0.03141862272484643 0.0012584043432927049 34-35 9.680033409943884E-5 0.0 0.0 0.05861951660678875 0.0014105191540203944 36-37 1.1062895325650152E-4 0.0 0.0 0.10110103465728533 0.0014105191540203944 38-39 1.1062895325650152E-4 0.0 0.0 0.16666251808091953 0.00143817639233452 40-41 1.1062895325650152E-4 0.0 0.0 0.23673213134975618 0.00143817639233452 42-43 1.1062895325650152E-4 0.0 0.0 0.3065804867120798 0.0014520050114915826 44-45 1.1062895325650152E-4 0.0 0.0 0.3906170053295498 0.0014796622498057079 46-47 1.1062895325650152E-4 1.382861915706269E-5 0.0 0.4878460266228576 0.0015488053455910214 48-49 1.1062895325650152E-4 2.765723831412538E-5 0.0 0.5893480912356978 0.001742406013789899 50-51 1.1062895325650152E-4 2.765723831412538E-5 0.0 0.6998802441580998 0.0018115491095752126 52-53 1.1062895325650152E-4 2.765723831412538E-5 0.0 0.8045905484153786 0.002198750445972968 54-55 1.1062895325650152E-4 2.765723831412538E-5 0.0 0.9145695565714981 0.0023093793992294692 56-57 1.1062895325650152E-4 2.765723831412538E-5 0.0 1.0433278295429087 0.002337036637543595 58-59 1.1062895325650152E-4 2.765723831412538E-5 0.0 1.1790004120928508 0.00236469387585772 60-61 1.1062895325650152E-4 2.765723831412538E-5 0.0 1.3248232011040768 0.0023785224950147827 62-63 1.1062895325650152E-4 2.765723831412538E-5 0.0 1.465128371071635 0.002461494209957159 64-65 1.1062895325650152E-4 2.765723831412538E-5 0.0 1.6132467108629336 0.002502980067428347 66-67 1.1062895325650152E-4 2.765723831412538E-5 0.0 1.7823707231538102 0.0025721231632136604 68-69 1.1062895325650152E-4 2.765723831412538E-5 0.0 1.968503937007874 0.0025997804015277857 70-71 1.1062895325650152E-4 2.765723831412538E-5 0.0 2.1574167033125073 0.002655094878156037 72-73 1.1062895325650152E-4 2.765723831412538E-5 0.0 2.352732120286861 0.0028348669271978516 74-75 1.1062895325650152E-4 2.765723831412538E-5 0.0 2.548904911648952 0.002917838642140228 76-77 1.1062895325650152E-4 2.765723831412538E-5 0.0 2.7572054020117873 0.0029316672612972904 78-79 1.1062895325650152E-4 2.765723831412538E-5 0.0 2.988641172224389 0.002986981737925541 80-81 1.1062895325650152E-4 2.765723831412538E-5 0.0 3.234251277072979 0.003042296214553792 82-83 1.1062895325650152E-4 2.765723831412538E-5 0.0 3.486678891166002 0.003125267929496168 84-85 1.1062895325650152E-4 2.765723831412538E-5 0.0 3.7425221741908183 0.003125267929496168 86-87 1.1062895325650152E-4 2.765723831412538E-5 0.0 4.007464688620982 0.0032497255019097323 88-89 1.1062895325650152E-4 2.765723831412538E-5 0.0 4.285392276439628 0.0034018403126374218 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7030 0.0 54.126396 1 GTATCAA 12605 0.0 43.032692 1 TATCAAC 15920 0.0 33.6346 2 ATCAACG 16065 0.0 33.183937 3 TCAACGC 16340 0.0 32.625454 4 CAACGCA 16720 0.0 31.940851 5 AACGCAG 17295 0.0 30.874216 6 ACGCAGA 19595 0.0 26.90573 7 CGCAGAG 19715 0.0 26.714888 8 GTGGTAT 3275 0.0 25.15663 1 TGGTATC 3165 0.0 24.94166 2 GCAGAGT 22095 0.0 23.319948 9 GTACATG 12800 0.0 23.06744 1 GAGTACT 13375 0.0 22.620224 12-13 TAAGGTG 2150 0.0 22.56133 5 TACATGG 12910 0.0 22.432726 2 GTACTTT 14030 0.0 21.360317 14-15 AGAGTAC 19260 0.0 21.309992 10-11 CAGAGTA 20635 0.0 21.202961 10-11 ACATGGG 12680 0.0 21.115051 3 >>END_MODULE