FastQCFastQC Report
Thu 26 May 2016
SRR938172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938172_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3615690
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149200.41264599564675064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125610.3474025704637289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81190.22454911787238396No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA45540.12595106328252698No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA44890.12415334279210882No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA42030.11624337263426898No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA41650.11519239757833222No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC37530.10379761539291256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69700.050.645461
GTATCAA119900.041.0511251
ATCAACG154350.031.6333243
TATCAAC155000.031.628052
TCAACGC155950.031.3053114
CAACGCA158200.030.7700145
AACGCAG164550.029.6394966
ACGCAGA186900.026.0188987
CGCAGAG188300.025.674138
GTGGTAT37000.025.5526451
TGGTATC34350.025.4457652
TAAGGTG21700.022.5417585
GCAGAGT215350.022.1845879
GTAAGGT19400.022.0318554
GTACATG125250.021.8488831
GAGTACT127650.021.7463312-13
TACATGG125200.021.6269322
AGAGTAC184950.021.23559610-11
ACATGGG122550.020.7735063
AGGTAAG18400.020.6536392
Produced by FastQC (version 0.11.5)