##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938172_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3615690 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53756101878203 34.0 31.0 34.0 31.0 34.0 2 32.74906504705879 34.0 31.0 34.0 31.0 34.0 3 32.80455957230847 34.0 31.0 34.0 31.0 34.0 4 36.25732100926794 37.0 35.0 37.0 35.0 37.0 5 36.2265174835232 37.0 37.0 37.0 35.0 37.0 6 36.25774720731036 37.0 37.0 37.0 35.0 37.0 7 36.216249457226695 37.0 37.0 37.0 35.0 37.0 8 36.19969272808233 37.0 37.0 37.0 35.0 37.0 9 37.89322397661304 39.0 38.0 39.0 35.0 39.0 10-11 37.87273328189087 39.0 38.0 39.0 35.0 39.0 12-13 37.790303510533256 39.0 38.0 39.0 35.0 39.0 14-15 39.087616886403424 40.0 38.5 41.0 36.0 41.0 16-17 38.97387912127422 40.0 38.0 41.0 36.0 41.0 18-19 39.21151827175449 40.5 39.0 41.0 36.0 41.0 20-21 39.186785786392086 40.5 39.0 41.0 36.0 41.0 22-23 39.09484510563682 40.0 39.0 41.0 35.5 41.0 24-25 38.990961752805134 40.0 38.5 41.0 35.0 41.0 26-27 38.82056218868321 40.0 38.0 41.0 35.0 41.0 28-29 38.707500228172215 40.0 38.0 41.0 34.5 41.0 30-31 38.47233377308342 40.0 38.0 41.0 34.0 41.0 32-33 38.266344321554115 40.0 38.0 41.0 34.0 41.0 34-35 38.11694116475694 40.0 38.0 41.0 34.0 41.0 36-37 38.12517749032688 40.0 38.0 41.0 34.0 41.0 38-39 37.986187007182586 40.0 38.0 41.0 33.0 41.0 40-41 37.81336522212911 40.0 37.0 41.0 33.0 41.0 42-43 37.5851746139741 40.0 37.0 41.0 33.0 41.0 44-45 37.317446047642356 40.0 37.0 41.0 32.0 41.0 46-47 36.9342985709505 40.0 36.0 41.0 31.0 41.0 48-49 36.53745025154258 39.0 36.0 41.0 30.5 41.0 50-51 36.15575104613504 39.0 35.0 40.5 30.0 41.0 52-53 35.627307650821834 38.5 35.0 40.0 28.0 41.0 54-55 35.299159496527636 38.0 34.5 40.0 27.5 41.0 56-57 34.93580340128717 38.0 34.0 40.0 27.0 41.0 58-59 34.473452784945614 37.5 34.0 40.0 26.0 41.0 60-61 33.971264682536386 37.0 33.0 39.5 25.0 41.0 62-63 33.48937643990497 36.0 33.0 39.0 23.5 40.5 64-65 32.978601871288745 36.0 32.0 39.0 22.5 40.0 66-67 32.41136491236804 35.0 31.5 38.5 21.0 40.0 68-69 31.812877763303824 35.0 31.0 38.0 18.5 40.0 70-71 31.188265448641893 35.0 30.5 37.0 16.5 39.0 72-73 30.607407852996246 34.0 30.0 36.5 11.5 39.0 74-75 30.010579170227537 34.0 29.0 36.0 7.5 38.5 76-77 28.68018815219225 32.5 27.0 34.5 7.0 36.5 78-79 29.368808581487905 34.0 29.0 35.0 4.5 37.0 80-81 29.057983676697948 34.0 29.0 35.0 2.0 36.5 82-83 28.5144973711795 33.5 28.0 35.0 2.0 36.0 84-85 27.93676670289765 33.0 26.5 35.0 2.0 36.0 86-87 27.310726721593944 33.0 26.0 35.0 2.0 35.0 88-89 26.557290862878176 32.0 25.0 34.5 2.0 35.0 90-91 26.018087418998864 32.0 24.0 34.0 2.0 35.0 92-93 25.19661917918848 31.5 19.5 34.0 2.0 35.0 94-95 24.366427154982865 31.0 15.5 34.0 2.0 35.0 96-97 23.306044351147364 31.0 2.0 34.0 2.0 35.0 98-99 21.83334716195249 30.0 2.0 34.0 2.0 35.0 100-101 17.424190818350027 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07334700308280873 1101 2 0.05575687796931561 1101 3 0.04775531603745975 1101 4 0.0371420737137953 1101 5 0.11110883998193799 1101 6 0.05656545220352882 1101 7 0.06753319105933997 1101 8 0.03042491859721963 1101 9 -0.019753890705544563 1101 10-11 0.03627239506975144 1101 12-13 0.1732599002603834 1101 14-15 0.09925395025705797 1101 16-17 0.17741187237076161 1101 18-19 0.12736779239959617 1101 20-21 0.10170843746693237 1101 22-23 0.18307188273078623 1101 24-25 0.10497586849032814 1101 26-27 0.11390280324651769 1101 28-29 0.12431952277771074 1101 30-31 0.2072622813404621 1101 32-33 0.14741008086740948 1101 34-35 0.1091999188726831 1101 36-37 0.19171260064813822 1101 38-39 0.15358312780749372 1101 40-41 0.09719400142549972 1101 42-43 0.15558511896658445 1101 44-45 0.1164997921879447 1101 46-47 0.031272205177707235 1101 48-49 0.25688880391756896 1101 50-51 0.07604230345940977 1101 52-53 -0.1262194541628716 1101 54-55 -0.19051389522500983 1101 56-57 -0.054200784284468284 1101 58-59 -0.15429024449521478 1101 60-61 0.008959009900145531 1101 62-63 -0.05157006694119559 1101 64-65 -0.00806107964662317 1101 66-67 -0.293528449229818 1101 68-69 -0.09693151101885888 1101 70-71 -0.3043350457002916 1101 72-73 -0.25330253712190043 1101 74-75 -0.25465889954024234 1101 76-77 -0.5508080166149263 1101 78-79 -0.5530385862860037 1101 80-81 -0.5378383132073594 1101 82-83 -0.49519211183935496 1101 84-85 -0.5735193988851357 1101 86-87 -0.6592656577222158 1101 88-89 -0.6005122298654548 1101 90-91 -0.9975955136784833 1101 92-93 -0.9696099743559365 1101 94-95 -1.3343016411177686 1101 96-97 -1.2676366676371593 1101 98-99 -1.1534838288176736 1101 100-101 -1.4006678205219778 1102 1 0.09635538158939028 1102 2 0.09848421625445525 1102 3 0.010380671662439056 1102 4 0.05700822147234419 1102 5 0.02556692915628389 1102 6 -0.0053242384415952415 1102 7 0.04296992803717359 1102 8 0.09130163449502504 1102 9 0.077008354393314 1102 10-11 0.07491928497293543 1102 12-13 0.09114301777793088 1102 14-15 0.16107639122166262 1102 16-17 0.13950700233077384 1102 18-19 0.13750991127631096 1102 20-21 0.18266251375980147 1102 22-23 0.15258366908321364 1102 24-25 0.10298946519145602 1102 26-27 0.13602017635556507 1102 28-29 0.08990534468405542 1102 30-31 0.015193228607188303 1102 32-33 -0.05543721350664299 1102 34-35 0.0031265008315415344 1102 36-37 0.04749999607012967 1102 38-39 0.017842872719725733 1102 40-41 -0.028465991803010127 1102 42-43 -0.02891083727629251 1102 44-45 -0.17373677019448763 1102 46-47 -0.16553879593952558 1102 48-49 -0.06302273737532715 1102 50-51 -0.02973758452969122 1102 52-53 -0.13694482655798623 1102 54-55 -0.032898566781156546 1102 56-57 -0.05034814948216848 1102 58-59 -0.03859108149996615 1102 60-61 -0.17406263455756488 1102 62-63 -0.16153106635604075 1102 64-65 -0.2783452645307989 1102 66-67 -0.40170807228056304 1102 68-69 -0.43604992057660397 1102 70-71 -0.45816049513646107 1102 72-73 -0.46949101555981443 1102 74-75 -0.6481765907529713 1102 76-77 -0.64676675491787 1102 78-79 -0.5932348094339766 1102 80-81 -0.5402312281362995 1102 82-83 -0.5660394916501126 1102 84-85 -0.6808313847449377 1102 86-87 -0.840095290384582 1102 88-89 -1.0208599001398504 1102 90-91 -1.2188118045117804 1102 92-93 -1.175570738608375 1102 94-95 -1.2081354777310764 1102 96-97 -1.3921219062265457 1102 98-99 -1.3272559987428103 1102 100-101 -1.627394567094516 1103 1 0.1152403308723251 1103 2 0.12490295745374169 1103 3 0.1166213174465085 1103 4 0.12468957149049942 1103 5 0.0722660532917061 1103 6 0.04884598071375024 1103 7 0.0026431433594495957 1103 8 0.06207662851718254 1103 9 0.1293313800750937 1103 10-11 0.11706770130769684 1103 12-13 0.14610243285916624 1103 14-15 0.1703851191932202 1103 16-17 0.16826746757998023 1103 18-19 0.14778822712697348 1103 20-21 0.11902274859036766 1103 22-23 0.07779573152328112 1103 24-25 0.233780021730567 1103 26-27 0.22006033417060422 1103 28-29 0.20320120278417164 1103 30-31 0.11557352604206983 1103 32-33 0.20733740493795239 1103 34-35 0.13865142782940865 1103 36-37 0.0987288451765096 1103 38-39 0.09566687981424593 1103 40-41 0.2602008849938642 1103 42-43 0.170830605955949 1103 44-45 0.2092525554324567 1103 46-47 0.22851285865471738 1103 48-49 0.1941852420174186 1103 50-51 0.11716974764591015 1103 52-53 0.037146189208222324 1103 54-55 0.10275094891299119 1103 56-57 0.19757641216286714 1103 58-59 0.17333469342562324 1103 60-61 0.04133184867912121 1103 62-63 0.11066876747202059 1103 64-65 0.025486210237076534 1103 66-67 0.039410031765292786 1103 68-69 0.13318944228606355 1103 70-71 -0.007193141400371417 1103 72-73 -0.14058193954500808 1103 74-75 -0.20590438075479867 1103 76-77 -0.20018274953016402 1103 78-79 -0.12285959618401776 1103 80-81 -0.0868281553241701 1103 82-83 -0.15185825877351178 1103 84-85 -0.2904579145290853 1103 86-87 -0.46515783632540675 1103 88-89 -0.5370348440044879 1103 90-91 -0.6498157820914479 1103 92-93 -0.5936116548243895 1103 94-95 -0.5823266575759618 1103 96-97 -0.8032693842212453 1103 98-99 -0.7412688154778699 1103 100-101 -1.3056751545251863 1104 1 0.10485194606553705 1104 2 0.11025713177176755 1104 3 0.11472631548250689 1104 4 0.06821250453482719 1104 5 0.0347476430917979 1104 6 0.07564798347311807 1104 7 0.08294757164622268 1104 8 0.10512776850379879 1104 9 0.1431990204075717 1104 10-11 0.12097731051201066 1104 12-13 0.20860970869033935 1104 14-15 0.24100612030252222 1104 16-17 0.10509597877405952 1104 18-19 0.11153182157909214 1104 20-21 0.1824256557684123 1104 22-23 0.09381992821518992 1104 24-25 0.2159701013647748 1104 26-27 0.1579146436782608 1104 28-29 0.09400379174083184 1104 30-31 0.036920148141220466 1104 32-33 0.15148310218977912 1104 34-35 0.19264109934112383 1104 36-37 0.1817107323938032 1104 38-39 0.26534513035518614 1104 40-41 0.2141366120850421 1104 42-43 0.22089752482639113 1104 44-45 0.32805340645122527 1104 46-47 0.298375905919265 1104 48-49 0.1876069987616944 1104 50-51 0.2735588568438416 1104 52-53 0.324788288538052 1104 54-55 0.29054813141074476 1104 56-57 0.38269655819771486 1104 58-59 0.3930807547130897 1104 60-61 0.10337248555750023 1104 62-63 0.18480085000079782 1104 64-65 0.1861113083434418 1104 66-67 0.013373211741004809 1104 68-69 0.004079348727326959 1104 70-71 -0.09306236007186186 1104 72-73 -0.3146008101407354 1104 74-75 -0.285899140278719 1104 76-77 -0.38633751875769917 1104 78-79 -0.30959286440100797 1104 80-81 -0.2933604153268874 1104 82-83 -0.3599122001898323 1104 84-85 -0.3446575524461757 1104 86-87 -0.31507175620151173 1104 88-89 -0.5940978735441753 1104 90-91 -0.7068962736259508 1104 92-93 -0.6779552021103434 1104 94-95 -0.7034362941186245 1104 96-97 -0.9673419589003736 1104 98-99 -0.9381661851381615 1104 100-101 -1.4998679334458167 1105 1 0.13483688606541477 1105 2 0.14859981560841362 1105 3 0.09496189045676573 1105 4 0.06597332030568737 1105 5 0.06710378567370867 1105 6 0.03109496163472869 1105 7 0.04989657275660875 1105 8 0.12030805394850574 1105 9 0.09913843832396196 1105 10-11 0.09544171057681439 1105 12-13 0.09959114303367045 1105 14-15 0.20142268991817502 1105 16-17 0.17087040764475603 1105 18-19 0.1569682973721882 1105 20-21 0.12392079707584713 1105 22-23 0.10237574303698693 1105 24-25 0.17365217892169227 1105 26-27 0.13669501832879405 1105 28-29 0.09928530805896685 1105 30-31 0.21016833673469648 1105 32-33 0.24299172344884568 1105 34-35 0.25944282046413036 1105 36-37 0.25182329824654204 1105 38-39 0.32125768397079923 1105 40-41 0.21194218720530955 1105 42-43 0.1606983292083939 1105 44-45 0.12655342070468834 1105 46-47 0.13918680279581253 1105 48-49 0.19018270376924562 1105 50-51 0.04387855853624245 1105 52-53 0.009737179444833544 1105 54-55 0.11997207597659099 1105 56-57 0.01586779026008145 1105 58-59 0.13201220216266307 1105 60-61 0.04688045046199818 1105 62-63 8.699083753285208E-4 1105 64-65 0.05368751396042626 1105 66-67 -0.0024143668181721978 1105 68-69 -0.004856877108711188 1105 70-71 -0.14600958506172645 1105 72-73 -0.26009039746474727 1105 74-75 -0.22747767303421895 1105 76-77 -0.15990514135659595 1105 78-79 -0.1898469601856725 1105 80-81 -0.13932380861051996 1105 82-83 -0.005766884746794432 1105 84-85 -0.18574100334160093 1105 86-87 -0.11544618771431914 1105 88-89 -0.38380628246142123 1105 90-91 -0.5046817026934178 1105 92-93 -0.4389889466618726 1105 94-95 -0.587319199836486 1105 96-97 -0.7439098484909685 1105 98-99 -0.9162956371724782 1105 100-101 -1.604256948336861 1106 1 0.09241557920872623 1106 2 0.13394354157571797 1106 3 0.12866084691685842 1106 4 0.11420324555381711 1106 5 0.11981199170850942 1106 6 0.0913670561295632 1106 7 0.139752845919503 1106 8 0.0978455259247113 1106 9 0.10329099795766439 1106 10-11 0.12531817754515373 1106 12-13 0.10124421186149846 1106 14-15 0.1403739384766638 1106 16-17 0.2338790128442696 1106 18-19 0.26619650766900094 1106 20-21 0.3160003730639218 1106 22-23 0.3371516541320574 1106 24-25 0.3372597848685004 1106 26-27 0.20706025548278006 1106 28-29 0.1382322257492703 1106 30-31 0.2756534646420903 1106 32-33 0.3677388478610979 1106 34-35 0.38867021894966314 1106 36-37 0.37273846505563313 1106 38-39 0.3591264601145667 1106 40-41 0.3598358087606428 1106 42-43 0.250568963095283 1106 44-45 0.1799943407767941 1106 46-47 0.30976375416904744 1106 48-49 0.2985145693188045 1106 50-51 0.17701814441101504 1106 52-53 0.2639325494386995 1106 54-55 0.13579043610332064 1106 56-57 0.1409232398694371 1106 58-59 0.32673424196895695 1106 60-61 0.04564896175234168 1106 62-63 0.15424977951478525 1106 64-65 0.10730773922355752 1106 66-67 -0.007184165771668916 1106 68-69 -0.32527733736221265 1106 70-71 -0.7270423485325423 1106 72-73 -0.6655284729356516 1106 74-75 -0.6135711284581724 1106 76-77 -0.6007144501500363 1106 78-79 -0.4443176755436191 1106 80-81 -0.440821591350975 1106 82-83 -0.4571302837281159 1106 84-85 -0.6411905039689465 1106 86-87 -0.7944729528420957 1106 88-89 -0.955415466366393 1106 90-91 -1.1127859711536097 1106 92-93 -1.1421160871798826 1106 94-95 -1.0330786547197128 1106 96-97 -1.3045945986161804 1106 98-99 -1.3559655561580648 1106 100-101 -1.7931450006493996 1107 1 0.044144745998195845 1107 2 0.02692018605984714 1107 3 0.029445831561986324 1107 4 0.023983797621859537 1107 5 0.012121588355782364 1107 6 0.07678729626677949 1107 7 0.061233014263123664 1107 8 0.019730196399152078 1107 9 -0.0013372726888931652 1107 10-11 0.07402377072653366 1107 12-13 0.002194116470711549 1107 14-15 0.04357832232684444 1107 16-17 -0.02236043866361115 1107 18-19 0.06866352858817493 1107 20-21 0.08489261192795539 1107 22-23 0.06759727941818028 1107 24-25 0.08826588260918555 1107 26-27 0.0793321378937506 1107 28-29 0.061441536322419665 1107 30-31 0.020404392516439884 1107 32-33 0.055132994540890934 1107 34-35 0.06933899580595693 1107 36-37 0.01393647904104256 1107 38-39 0.03674796302541239 1107 40-41 0.07202210470982351 1107 42-43 0.07604864318738436 1107 44-45 0.020586117324604913 1107 46-47 0.031884298260017374 1107 48-49 -0.01682971676317635 1107 50-51 -0.1013364234421843 1107 52-53 -0.105571059889364 1107 54-55 -0.07649827477516169 1107 56-57 -0.09986727567306986 1107 58-59 -0.08949317025874137 1107 60-61 -0.0539801341080377 1107 62-63 -0.05882927007466776 1107 64-65 -0.10891031566887932 1107 66-67 -0.25782874126792166 1107 68-69 -0.09810420062624914 1107 70-71 -0.3737078952661683 1107 72-73 -0.49449541821022436 1107 74-75 -0.4367990309021792 1107 76-77 -0.4117228024927613 1107 78-79 -0.48033346954667167 1107 80-81 -0.45668853741146265 1107 82-83 -0.39662209446336405 1107 84-85 -0.48665497163726457 1107 86-87 -0.5518663528998609 1107 88-89 -0.7266894049351187 1107 90-91 -0.9732570503564517 1107 92-93 -0.9863998359690775 1107 94-95 -0.9698865557490706 1107 96-97 -0.8908251446916005 1107 98-99 -1.271580443604794 1107 100-101 -1.661748119957636 1108 1 -0.051465708858494 1108 2 -0.021355580668128482 1108 3 -0.028092921603402488 1108 4 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2592.0 11 4170.0 12 6721.0 13 10030.0 14 13223.0 15 18628.0 16 24923.0 17 34473.0 18 35765.0 19 29536.0 20 28326.0 21 29509.0 22 31788.0 23 34545.0 24 39338.0 25 44734.0 26 51215.0 27 61140.0 28 73284.0 29 88126.0 30 106833.0 31 130986.0 32 163924.0 33 212710.0 34 289275.0 35 413323.0 36 585249.0 37 696063.0 38 337477.0 39 14864.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72742100862348 18.07464423351127 14.610372457758736 23.58756230010652 2 16.08307502167273 19.309144703029645 39.19411740949595 25.413662865801673 3 19.658433137252135 22.803255794269912 29.421058440162874 28.117252628315075 4 13.521236610439502 16.029499210385847 35.11863572374844 35.33062845542621 5 14.38514343242137 36.50465754333949 33.69796218650087 15.41223683773827 6 34.175554680959394 34.86077280434955 16.135275748090862 14.828396766600187 7 29.95278909419779 32.12034217535242 19.266281124764568 18.660587605685222 8 27.477245007177054 34.339503663201214 18.594459148876147 19.588792180745585 9 26.515796431663112 16.496961852371193 19.351741991155215 37.63549972481048 10-11 25.884976311575386 25.820106259109604 26.120187294817864 22.17473013449715 12-13 26.638598020637726 23.06125618300064 26.83474480934944 23.46540098701219 14-15 23.195185427954275 24.483528731722025 24.95731105266215 27.363974787661554 16-17 22.916490634927065 27.750959491103593 26.006872740080905 23.32567713388844 18-19 23.10632272125099 25.950980310812042 28.328603945581616 22.614093022355345 20-21 23.307349357937213 25.164906283447973 28.8123843581723 22.715360000442516 22-23 23.190829412919804 25.000484001670497 28.817210546258114 22.991476039151586 24-25 23.077124130943993 25.318311598117138 28.684843099037742 22.919721171901124 26-27 22.57898941570322 25.679326402539655 28.54902574787454 23.192658433882585 28-29 22.840022647293846 26.0171160214668 28.414083695967545 22.728777635271804 30-31 22.78904918193295 25.56387099058225 28.330533632982167 23.316546194502628 32-33 22.594000038720132 25.56766481639742 28.37035254681679 23.467982598065653 34-35 23.22671806600271 25.68065650048135 28.22859685980741 22.86402857370853 36-37 23.311644787053197 25.248153412803457 28.244981236464252 23.1952205636791 38-39 22.88580138500677 25.662342231311285 28.13872360103972 23.31313278264222 40-41 23.27916682397067 25.494435863597587 28.27948419775858 22.946913114673166 42-43 23.004803261012217 25.50486929496072 28.040863328534176 23.44946411549289 44-45 22.98144281767597 25.721390860773703 28.222394127371018 23.07477219417931 46-47 23.458631893716376 26.006725145228398 27.506897726681867 23.02774523437336 48-49 23.17007315793848 26.125652213054256 27.342508118480374 23.361766510526884 50-51 23.192517869008775 26.327769169123073 27.386281054252503 23.093431907615653 52-53 23.625241034445228 25.989539488851392 27.270598755630935 23.11462072107244 54-55 23.181609857950782 26.19784275040611 27.335008188498378 23.285539203144737 56-57 23.153233571842907 26.468075889694553 27.155693815560284 23.22299672290226 58-59 23.13615106595165 26.046829117154402 27.159836728822395 23.657183088071548 60-61 22.81947003595011 26.2654726811791 27.437474519895368 23.477582762975423 62-63 22.94509530223324 26.244234742555477 27.61976368841482 23.19090626679646 64-65 22.671167072806053 26.401042564274356 27.416856688630194 23.5109336742894 66-67 22.686021542852387 26.399398873593338 27.313826322266486 23.600753261287785 68-69 23.117099234001348 25.973904172358342 27.580405650753033 23.328590942887278 70-71 23.031732304058405 26.18666446848035 27.00726383477753 23.774339392683718 72-73 22.93852157038892 26.323021469711623 26.978682410679315 23.75977454922014 74-75 22.79155415839293 26.724856777999083 26.894104997518053 23.58948406608993 76-77 22.947562702181443 26.30210797063082 27.128510356724554 23.62181897046318 78-79 22.960293143087075 26.45605018433484 27.1399695719224 23.443687100655687 80-81 23.152138164946326 26.480323892552516 26.808860630995223 23.558677311505935 82-83 22.99303611478811 26.535015791547973 26.944111712112008 23.527836381551907 84-85 22.78090242367801 26.680749127534288 26.878320373310622 23.660028075477086 86-87 23.110001774759873 26.61612773955212 26.68097633095578 23.592894154732225 88-89 22.906674748032486 26.741676568967804 26.389025092115258 23.96262359088445 90-91 22.81304508402507 26.683897258274904 26.596185846938674 23.906871810761352 92-93 22.916502700888106 26.360178260136475 26.73843953082711 23.98487950814831 94-95 22.689202662838717 26.153443363365625 26.90161125448095 24.25574271931471 96-97 22.94400680249434 25.832715677063533 26.90437408518525 24.318903435256875 98-99 22.61269691464432 26.24182910718751 26.501588262238634 24.643885715929535 100-101 23.195642745395684 25.42752974576965 26.53493316191391 24.841894346920757 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1467.0 1 1005.5 2 745.5 3 978.0 4 803.0 5 781.5 6 1581.0 7 3418.0 8 5562.5 9 6378.0 10 5205.5 11 3861.5 12 3614.0 13 3727.5 14 3921.5 15 4181.5 16 4334.5 17 4485.5 18 4785.0 19 5151.5 20 5654.5 21 6671.5 22 8159.0 23 9829.5 24 12068.5 25 14937.0 26 18262.0 27 22097.0 28 25319.5 29 28551.0 30 32802.0 31 39443.0 32 49554.5 33 52913.5 34 51549.0 35 54305.5 36 59707.5 37 66044.5 38 72567.5 39 78299.0 40 84214.0 41 93025.5 42 98443.5 43 101935.5 44 108360.0 45 117161.5 46 123130.0 47 129241.5 48 134904.5 49 135483.0 50 137595.0 51 139994.5 52 142718.5 53 147923.0 54 150832.5 55 143541.0 56 133521.0 57 125096.5 58 116886.0 59 104272.0 60 84081.5 61 68881.0 62 57978.0 63 43529.0 64 30862.0 65 23331.5 66 17393.5 67 13111.0 68 9870.5 69 7274.5 70 5330.5 71 3591.0 72 2431.0 73 1774.5 74 1315.0 75 983.5 76 682.0 77 433.5 78 258.5 79 138.0 80 69.0 81 39.0 82 27.0 83 20.0 84 9.5 85 3.5 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009043916928719 2 0.01515616659614071 3 0.009707690648258008 4 0.0 5 0.001382861915706269 6 0.0014658336306486452 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.435457685808242E-4 14-15 0.0 16-17 0.0028072096888837263 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003567783742522174 26-27 0.01969195367965727 28-29 0.006831337863588968 30-31 0.0034433261701086104 32-33 0.0 34-35 0.009361975169331441 36-37 9.265174835232003E-4 38-39 0.003097610691182043 40-41 0.0021987504459729675 42-43 0.0034709834084227353 44-45 2.765723831412538E-5 46-47 1.6594342988475227E-4 48-49 5.393161471254449E-4 50-51 0.003941156459762866 52-53 0.007840827062054545 54-55 0.006997281293473721 56-57 0.016622000226789356 58-59 0.02337036637543595 60-61 0.003429497550951547 62-63 0.0198302398712279 64-65 0.020936529403792915 66-67 0.013192502675837806 68-69 0.031183536199176364 70-71 0.016276284747862788 72-73 0.004922988419914317 74-75 0.01619331303292041 76-77 0.020576985305709283 78-79 0.02590100368117842 80-81 0.011533068376990283 82-83 0.03475131994169854 84-85 0.02189070412563024 86-87 0.030879306577720988 88-89 0.021835389649001988 90-91 0.027518952122554754 92-93 0.023799053569304892 94-95 0.014533878734072886 96-97 0.015087023500355397 98-99 0.011560725615304409 100-101 0.010675693989252397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3615690.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.10220766303867 #Duplication Level Percentage of deduplicated Percentage of total 1 69.50268983723193 30.6522206034137 2 15.171447803901305 13.381886831932146 3 5.889539947875077 7.792251414628461 4 2.915052104729648 5.142409330854595 5 1.6724460025519443 3.6879280454882375 6 1.0527886412720435 2.7858181969600793 7 0.7251564267561281 2.23866995246901 8 0.5091115334655182 1.7962354058035475 9 0.3806190504429635 1.5107526362829753 >10 1.9552046354686603 15.52693271470491 >50 0.14558279789413844 4.421220864787122 >100 0.07234930797640023 5.704400212142701 >500 0.004444106577226013 1.3492179250286565 >1k 0.0033800247067716383 3.0273208221218084 >5k 6.259305012540071E-5 0.22412432070839605 >10k+ 1.2518610025080143E-4 0.7586107226736584 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14920 0.41264599564675064 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12561 0.3474025704637289 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8119 0.22454911787238396 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4554 0.12595106328252698 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4489 0.12415334279210882 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4203 0.11624337263426898 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4165 0.11519239757833222 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3753 0.10379761539291256 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.382861915706269E-4 2 0.0 0.0 0.0 0.0 1.382861915706269E-4 3 0.0 0.0 0.0 0.0 1.382861915706269E-4 4 0.0 0.0 0.0 0.0 1.382861915706269E-4 5 0.0 0.0 0.0 0.0 1.382861915706269E-4 6 0.0 0.0 0.0 0.0 1.659434298847523E-4 7 0.0 0.0 0.0 0.0 1.659434298847523E-4 8 0.0 0.0 0.0 0.0 1.659434298847523E-4 9 0.0 0.0 0.0 0.0 1.9360066819887767E-4 10-11 0.0 0.0 0.0 1.382861915706269E-5 1.9360066819887767E-4 12-13 0.0 0.0 0.0 5.531447662825076E-5 6.499451003819465E-4 14-15 0.0 0.0 0.0 6.914309578531346E-5 8.297171494237614E-4 16-17 0.0 0.0 0.0 1.1062895325650152E-4 9.265174835232003E-4 18-19 0.0 0.0 0.0 1.659434298847523E-4 9.680033409943884E-4 20-21 0.0 0.0 0.0 2.3508652567006574E-4 0.0010509750559367643 22-23 0.0 0.0 0.0 3.4571547892656726E-4 0.001161604009193266 24-25 0.0 0.0 0.0 5.531447662825076E-4 0.0011754326283503287 26-27 0.0 0.0 0.0 0.0011754326283503287 0.0012169184858215167 28-29 0.0 0.0 0.0 0.004134757127961745 0.0012445757241356422 30-31 0.0 0.0 0.0 0.013800961918748567 0.0012584043432927049 32-33 0.0 0.0 0.0 0.03155690891641706 0.0012998902007638929 34-35 0.0 0.0 0.0 0.05886843175161587 0.0013966905348633317 36-37 0.0 0.0 0.0 0.10161269356609665 0.00143817639233452 38-39 0.0 0.0 0.0 0.16803155137746875 0.0014520050114915826 40-41 0.0 0.0 0.0 0.23852985184017433 0.0014658336306486452 42-43 2.765723831412538E-5 0.0 0.0 0.3085579792515398 0.0014934908689627708 44-45 2.765723831412538E-5 0.0 0.0 0.39295404196709344 0.001562633964748084 46-47 2.765723831412538E-5 0.0 0.0 0.49040432116691424 0.0016732629180045856 48-49 2.765723831412538E-5 0.0 0.0 0.5923903874502516 0.0019221780628317139 50-51 2.765723831412538E-5 0.0 0.0 0.7038352292370198 0.0021572645885017797 52-53 2.765723831412538E-5 0.0 0.0 0.8093061075479369 0.002226407684287093 54-55 2.765723831412538E-5 0.0 0.0 0.9198797463278101 0.002323208018386532 56-57 2.765723831412538E-5 0.0 0.0 1.0492326499229745 0.002323208018386532 58-59 2.765723831412538E-5 0.0 0.0 1.1857487782414975 0.002323208018386532 60-61 2.765723831412538E-5 0.0 0.0 1.3330512295025292 0.002323208018386532 62-63 2.765723831412538E-5 0.0 0.0 1.4743935459068669 0.0024338369716430334 64-65 2.765723831412538E-5 0.0 0.0 1.6220278840276683 0.002475322829114222 66-67 2.765723831412538E-5 0.0 0.0 1.7917741841806127 0.0024891514482712845 68-69 2.765723831412538E-5 0.0 0.0 1.979511517856896 0.002502980067428347 70-71 2.765723831412538E-5 0.0 0.0 2.1692401726917963 0.002544465924899535 72-73 2.765723831412538E-5 0.0 0.0 2.3655374216263008 0.002682752116470162 74-75 2.765723831412538E-5 0.0 0.0 2.5640195923876217 0.0028486955463549145 76-77 2.765723831412538E-5 0.0 0.0 2.7745050045772732 0.002986981737925541 78-79 2.765723831412538E-5 0.0 0.0 3.008319297284889 0.0030146389762396664 80-81 2.765723831412538E-5 0.0 0.0 3.257566328971787 0.003069953452867917 82-83 2.765723831412538E-5 0.0 0.0 3.5138521278096295 0.0033050399785379833 84-85 2.765723831412538E-5 0.0 0.0 3.774646056492675 0.0033326972168521082 86-87 2.765723831412538E-5 0.0 0.0 4.04579762092436 0.003484812027579798 88-89 2.765723831412538E-5 0.0 0.0 4.329713000838014 0.0035954409808362994 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6970 0.0 50.64546 1 GTATCAA 11990 0.0 41.051125 1 ATCAACG 15435 0.0 31.633324 3 TATCAAC 15500 0.0 31.62805 2 TCAACGC 15595 0.0 31.305311 4 CAACGCA 15820 0.0 30.770014 5 AACGCAG 16455 0.0 29.639496 6 ACGCAGA 18690 0.0 26.018898 7 CGCAGAG 18830 0.0 25.67413 8 GTGGTAT 3700 0.0 25.552645 1 TGGTATC 3435 0.0 25.445765 2 TAAGGTG 2170 0.0 22.541758 5 GCAGAGT 21535 0.0 22.184587 9 GTAAGGT 1940 0.0 22.031855 4 GTACATG 12525 0.0 21.848883 1 GAGTACT 12765 0.0 21.74633 12-13 TACATGG 12520 0.0 21.626932 2 AGAGTAC 18495 0.0 21.235596 10-11 ACATGGG 12255 0.0 20.773506 3 AGGTAAG 1840 0.0 20.653639 2 >>END_MODULE