FastQCFastQC Report
Thu 26 May 2016
SRR938171_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938171_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078518
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108781.0086062541376222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88130.8171398159326039No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56140.5205290964082194No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26190.24283322114234535No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22080.2047253731509349No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20030.18571780906762797No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16440.15243139196564173No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12500.11589978099577385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43550.051.0665631
GTATCAA74350.041.401641
TATCAAC93150.032.9664082
ATCAACG95300.032.3224333
TCAACGC96900.031.788734
CAACGCA97700.031.5284355
AACGCAG99850.030.7933716
TATAACG650.00582236229.2528762
GTGGTAT20550.028.5648611
TGGTATC20350.027.5638282
ACGCAGA113050.027.0221887
CGCAGAG114700.026.5920478
GCAGAGT120250.025.085839
GAGTACT72250.024.39246712-13
GTACATG68750.023.1158771
GTACTTT76300.023.03759614-15
TACATGG69100.022.5640872
GTCTTAG3250.022.0265641
CAGAGTA117750.021.74491110-11
CCGTATA1100.002864654921.607242
Produced by FastQC (version 0.11.5)