##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938171_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1078518 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.897195039860254 33.0 31.0 34.0 30.0 34.0 2 32.023981982683644 33.0 31.0 34.0 30.0 34.0 3 32.09364053265685 34.0 31.0 34.0 30.0 34.0 4 35.639128878702074 37.0 35.0 37.0 33.0 37.0 5 35.661542041950156 37.0 35.0 37.0 35.0 37.0 6 35.71768204146802 37.0 35.0 37.0 35.0 37.0 7 35.66004554397794 37.0 35.0 37.0 33.0 37.0 8 35.635095566323415 37.0 35.0 37.0 33.0 37.0 9 37.25944212335816 39.0 37.0 39.0 34.0 39.0 10-11 37.160421986466616 39.0 37.0 39.0 34.0 39.0 12-13 37.0128120253904 39.0 37.0 39.0 33.0 39.0 14-15 38.124398480136634 40.0 38.0 41.0 33.0 41.0 16-17 37.9521982943261 40.0 38.0 41.0 33.0 41.0 18-19 38.1837553939758 40.0 38.0 41.0 33.5 41.0 20-21 38.19790814803277 40.0 38.0 41.0 33.5 41.0 22-23 38.07274843813455 40.0 38.0 41.0 33.0 41.0 24-25 37.92479726810308 40.0 38.0 41.0 33.0 41.0 26-27 37.70995245327384 40.0 37.5 41.0 32.5 41.0 28-29 37.484875078580046 40.0 37.0 41.0 32.0 41.0 30-31 37.17692379728479 39.5 36.5 41.0 31.5 41.0 32-33 36.793458709080426 39.0 35.5 41.0 30.5 41.0 34-35 36.47278858581869 39.0 35.0 41.0 30.0 41.0 36-37 36.14726828852184 39.0 35.0 40.0 30.0 41.0 38-39 35.80404499507658 38.0 35.0 40.0 29.0 41.0 40-41 35.59446805709316 38.0 35.0 40.0 27.5 41.0 42-43 35.25752004139013 38.0 35.0 40.0 26.5 41.0 44-45 34.80245670447781 38.0 34.5 40.0 24.5 41.0 46-47 34.19126199099134 38.0 33.5 40.0 22.0 41.0 48-49 33.596385966669075 38.0 33.0 40.0 17.5 41.0 50-51 32.72431521773396 37.5 32.0 39.5 13.5 40.5 52-53 32.68774420083856 37.0 32.0 39.5 10.5 40.5 54-55 32.66211273247178 37.0 32.0 40.0 7.5 41.0 56-57 32.185897685527735 37.0 31.0 40.0 4.0 41.0 58-59 31.727538158843895 36.0 31.0 40.0 2.0 41.0 60-61 31.171359680598748 36.0 30.0 39.0 2.0 41.0 62-63 30.558944774217956 35.0 29.0 39.0 2.0 40.5 64-65 29.94881912031139 35.0 28.0 38.5 2.0 40.0 66-67 29.270967197580383 35.0 27.5 38.0 2.0 40.0 68-69 28.588130193469187 34.0 26.0 37.5 2.0 40.0 70-71 27.784203416169227 34.0 25.0 37.0 2.0 39.0 72-73 27.004371276140038 33.5 22.5 36.0 2.0 39.0 74-75 26.192802530880336 33.0 19.0 35.5 2.0 38.5 76-77 25.373589963264404 32.0 15.0 35.0 2.0 37.5 78-79 24.570377592214502 31.5 7.5 35.0 2.0 37.0 80-81 23.776800665357463 31.0 2.0 35.0 2.0 36.0 82-83 22.97394109324091 30.5 2.0 35.0 2.0 36.0 84-85 22.178223265629317 30.0 2.0 34.0 2.0 35.0 86-87 21.420892836280895 29.0 2.0 34.0 2.0 35.0 88-89 20.612202114382885 28.5 2.0 34.0 2.0 35.0 90-91 19.77596711413254 26.5 2.0 34.0 2.0 35.0 92-93 18.88779232242763 25.5 2.0 34.0 2.0 35.0 94-95 17.970707952950253 23.5 2.0 33.0 2.0 35.0 96-97 16.890286022115532 14.5 2.0 33.0 2.0 35.0 98-99 15.629201367061096 2.0 2.0 32.0 2.0 34.5 100-101 12.668451986893125 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4097477589054641 1101 2 0.46144385328377524 1101 3 0.33677582437115916 1101 4 0.3028644189258358 1101 5 0.3316778030072811 1101 6 0.3507838544188644 1101 7 0.3380735381600175 1101 8 0.35341473826935044 1101 9 0.4519436877311662 1101 10-11 0.4524810886793702 1101 12-13 0.431411473757187 1101 14-15 0.48725740377005167 1101 16-17 0.6433025307317095 1101 18-19 0.6396892373560306 1101 20-21 0.6288074708035225 1101 22-23 0.8007153775923612 1101 24-25 0.9585680832336507 1101 26-27 0.9679621526725484 1101 28-29 0.9347740877754092 1101 30-31 0.9326619810442196 1101 32-33 1.0493506657824057 1101 34-35 0.8943537206058068 1101 36-37 0.9165879570880975 1101 38-39 1.2550182500531264 1101 40-41 1.3562140699407692 1101 42-43 1.5469494101643022 1101 44-45 1.5100216421856203 1101 46-47 1.682928869995905 1101 48-49 1.7131145238905106 1101 50-51 1.8589333916581552 1101 52-53 1.9791655486557715 1101 54-55 1.739551010774072 1101 56-57 1.8993728863274413 1101 58-59 2.218908366598658 1101 60-61 2.4859973429082878 1101 62-63 2.7861383091190355 1101 64-65 2.9314379648726607 1101 66-67 3.016230111214849 1101 68-69 2.9470433360898944 1101 70-71 3.209428985215702 1101 72-73 3.8142124311382695 1101 74-75 3.9273125742996733 1101 76-77 3.8265313115454553 1101 78-79 4.213359865352608 1101 80-81 4.8261412884226225 1101 82-83 5.020553573320878 1101 84-85 5.284607515758598 1101 86-87 5.155845571510746 1101 88-89 5.15923013410028 1101 90-91 5.15087176716656 1101 92-93 5.390285523368515 1101 94-95 5.793386009517615 1101 96-97 5.534635359601765 1101 98-99 5.294424048883798 1101 100-101 4.766862200070527 1102 1 0.3090918514961736 1102 2 0.2636979516444313 1102 3 0.2738451995196485 1102 4 0.10865897480948661 1102 5 0.015944934663934873 1102 6 0.1276522995873819 1102 7 0.23041732528285053 1102 8 0.1505777053806412 1102 9 0.398486809612848 1102 10-11 0.42772228102230514 1102 12-13 0.42026507827811344 1102 14-15 0.5679200145021568 1102 16-17 0.6023093783032394 1102 18-19 0.4901902895231558 1102 20-21 0.4168403425409579 1102 22-23 0.5388519811289925 1102 24-25 0.5808252362730499 1102 26-27 0.5590096105010147 1102 28-29 0.6934481026415114 1102 30-31 0.6948658469098774 1102 32-33 0.7749821781877984 1102 34-35 0.7696952506604475 1102 36-37 0.8115844357223168 1102 38-39 1.0548869412929776 1102 40-41 1.2833695276334751 1102 42-43 1.4744591257397701 1102 44-45 1.6643985301467836 1102 46-47 1.7202935307568552 1102 48-49 1.7470066088784648 1102 50-51 1.8316555346850052 1102 52-53 1.7606138906440307 1102 54-55 1.9288763934574433 1102 56-57 2.15354655329957 1102 58-59 2.1793683078809387 1102 60-61 2.3894302502836346 1102 62-63 2.5145063045709612 1102 64-65 2.3566895516085715 1102 66-67 2.5166183099726105 1102 68-69 2.956652846323067 1102 70-71 3.2065227977941895 1102 72-73 3.323724361355829 1102 74-75 3.7223881775987238 1102 76-77 3.9464719998664 1102 78-79 4.003441917417909 1102 80-81 4.081162416617307 1102 82-83 4.351794239580254 1102 84-85 4.20741854145184 1102 86-87 4.394326887059059 1102 88-89 4.462405939347541 1102 90-91 4.601730683434418 1102 92-93 4.873536762210453 1102 94-95 4.941986584815449 1102 96-97 4.669065619588871 1102 98-99 4.342393111776239 1102 100-101 3.520288107140413 1103 1 0.5584223576253997 1103 2 0.34873283044365877 1103 3 0.39425031453421866 1103 4 0.4040237980956647 1103 5 0.41942691327269443 1103 6 0.350601898298045 1103 7 0.37551435102071906 1103 8 0.3515550850345406 1103 9 0.28271524336347653 1103 10-11 0.5078188794236596 1103 12-13 0.4001767010173012 1103 14-15 0.6811794525342947 1103 16-17 0.6645158388173442 1103 18-19 0.5443714720650306 1103 20-21 0.6597333293382874 1103 22-23 0.7553106434436145 1103 24-25 0.7340141841298617 1103 26-27 0.8341344538109539 1103 28-29 0.8378624074260514 1103 30-31 1.017056211081993 1103 32-33 0.920738014383609 1103 34-35 1.0804675901854068 1103 36-37 1.2209255031682318 1103 38-39 1.2839204892442098 1103 40-41 1.2724556583251925 1103 42-43 1.312761602663599 1103 44-45 1.4145445367888243 1103 46-47 1.4445658377228483 1103 48-49 1.558171691008468 1103 50-51 1.7058995560436898 1103 52-53 1.6560212440879525 1103 54-55 1.752663673480889 1103 56-57 1.923406000285425 1103 58-59 2.28959009553132 1103 60-61 2.546898159346423 1103 62-63 2.585232023717264 1103 64-65 2.334403952442077 1103 66-67 2.833214635692567 1103 68-69 3.1317041267046584 1103 70-71 3.263669899231669 1103 72-73 3.482298766747597 1103 74-75 3.687317076942662 1103 76-77 3.8498725810440604 1103 78-79 3.776559805317369 1103 80-81 4.17514083815832 1103 82-83 4.3394141990443345 1103 84-85 4.354737208324714 1103 86-87 4.264491879651892 1103 88-89 4.730519411436358 1103 90-91 4.929627516260378 1103 92-93 5.192498192037725 1103 94-95 5.273862734554161 1103 96-97 5.166101226895101 1103 98-99 5.02516188609372 1103 100-101 4.104954662563923 1104 1 0.4845638805833694 1104 2 0.3768135030892239 1104 3 0.25953283108180614 1104 4 0.2667010109133301 1104 5 0.2918307865488643 1104 6 0.29259935578436114 1104 7 0.33860926251573886 1104 8 0.3613841415610324 1104 9 0.4534239787140777 1104 10-11 0.5364105664085415 1104 12-13 0.35830926721732936 1104 14-15 0.504869845464718 1104 16-17 0.7899389470982854 1104 18-19 0.681374030034732 1104 20-21 0.6585200325326639 1104 22-23 0.644793408060174 1104 24-25 0.7115860642375864 1104 26-27 0.8242329472363537 1104 28-29 0.8393345924035813 1104 30-31 0.9979810794161068 1104 32-33 0.9915115683772484 1104 34-35 1.0112473652231841 1104 36-37 0.9821547776244657 1104 38-39 0.9149067952191174 1104 40-41 1.0211003594252688 1104 42-43 0.8644073779563968 1104 44-45 1.0736066854060908 1104 46-47 1.1800115890588287 1104 48-49 1.1910209292203646 1104 50-51 1.422684589988954 1104 52-53 1.5120451274878093 1104 54-55 1.4806461038612255 1104 56-57 1.6968006038194403 1104 58-59 1.9018219820532956 1104 60-61 2.1588117486570546 1104 62-63 2.2157106963551065 1104 64-65 2.383755476211494 1104 66-67 2.444390683513465 1104 68-69 2.585320639906019 1104 70-71 2.7463103847049517 1104 72-73 3.021370594691291 1104 74-75 2.9702712228242127 1104 76-77 2.814260001397308 1104 78-79 3.1978389640467313 1104 80-81 3.2226236336869007 1104 82-83 3.5746426002359932 1104 84-85 3.7110803549365627 1104 86-87 3.8939760546293876 1104 88-89 3.9780797768164433 1104 90-91 4.276686430707496 1104 92-93 4.477688146403853 1104 94-95 4.445245738352476 1104 96-97 4.051526788817675 1104 98-99 4.028928322594634 1104 100-101 3.5978260608305312 1105 1 0.5208087151383118 1105 2 0.4653198222760224 1105 3 0.44319645126701346 1105 4 0.38038981735618904 1105 5 0.3587855116114511 1105 6 0.32934565325366805 1105 7 0.4190054559220684 1105 8 0.42434978569580295 1105 9 0.6066454066391529 1105 10-11 0.4784633218152905 1105 12-13 0.5182325773250085 1105 14-15 0.6268855755645504 1105 16-17 0.834037520379745 1105 18-19 0.7225260373945517 1105 20-21 0.6411064500514385 1105 22-23 0.7378091230784207 1105 24-25 0.5675135067013102 1105 26-27 0.6422754320793516 1105 28-29 0.671280419327239 1105 30-31 0.8280228554243081 1105 32-33 0.9645909277112494 1105 34-35 0.9902839531639387 1105 36-37 1.0953760547336273 1105 38-39 0.9410978639006871 1105 40-41 1.1107340275699258 1105 42-43 1.081878270484978 1105 44-45 1.147167147506046 1105 46-47 1.4672197602650527 1105 48-49 1.2774742667765437 1105 50-51 1.4767502099931775 1105 52-53 1.4637369050041755 1105 54-55 1.510053321910867 1105 56-57 1.9535251083891652 1105 58-59 2.079566077610256 1105 60-61 2.2030064670837355 1105 62-63 2.051308153598793 1105 64-65 2.0892874039405065 1105 66-67 2.3736919533834637 1105 68-69 2.5215594418243974 1105 70-71 2.7877036975273235 1105 72-73 3.1528481382016835 1105 74-75 3.2137069601632184 1105 76-77 3.5250874529099896 1105 78-79 3.6137402399493617 1105 80-81 3.684614252937042 1105 82-83 3.806716243646843 1105 84-85 3.620149669930683 1105 86-87 3.9274553023596077 1105 88-89 4.199479423907203 1105 90-91 4.4987911421966515 1105 92-93 4.670044418837723 1105 94-95 4.6164727012216 1105 96-97 4.519272920389476 1105 98-99 4.309828618193826 1105 100-101 3.9224516603237873 1106 1 0.19324784656722116 1106 2 0.07657230237987278 1106 3 0.05554607768407749 1106 4 0.10578700276100506 1106 5 0.19531255488512045 1106 6 0.17133630077282191 1106 7 0.13101745630560657 1106 8 0.03492185069111997 1106 9 0.23502812077629898 1106 10-11 0.2242231740047913 1106 12-13 0.1562254633914364 1106 14-15 0.38561803044326126 1106 16-17 0.42724240461697605 1106 18-19 0.4360800354325889 1106 20-21 0.504421841815649 1106 22-23 0.5374720502279047 1106 24-25 0.4742629848653408 1106 26-27 0.4340227712690492 1106 28-29 0.47089171606198477 1106 30-31 0.4664329336812614 1106 32-33 0.4093307104209529 1106 34-35 0.6132722109324789 1106 36-37 0.8149141100371793 1106 38-39 0.9752472343784149 1106 40-41 0.9641414505484178 1106 42-43 0.9416883258770312 1106 44-45 1.211934736881986 1106 46-47 1.1912326051746618 1106 48-49 1.2063768807506818 1106 50-51 1.4997068441326817 1106 52-53 1.4383905202395795 1106 54-55 1.3357863816030076 1106 56-57 1.2015848189638092 1106 58-59 1.7350602913159214 1106 60-61 2.0784135175856555 1106 62-63 2.118004046768089 1106 64-65 2.340940499183862 1106 66-67 2.357305750505436 1106 68-69 2.2650457118219656 1106 70-71 2.6905329520578896 1106 72-73 2.967790902199056 1106 74-75 2.9393936446792566 1106 76-77 2.851534118691564 1106 78-79 3.129887652159219 1106 80-81 3.263253828387519 1106 82-83 3.368291506039988 1106 84-85 3.241924612952829 1106 86-87 3.670537287770326 1106 88-89 3.6631754055857826 1106 90-91 3.668545953044937 1106 92-93 3.745948965264528 1106 94-95 3.3293425962643433 1106 96-97 3.4941819217835004 1106 98-99 3.3657383645409737 1106 100-101 2.684722642555551 1107 1 0.5507797394477869 1107 2 0.38086132901192826 1107 3 0.3326109165338096 1107 4 0.18045190812159717 1107 5 0.3171207264831324 1107 6 0.2595518698563595 1107 7 0.2929630836785222 1107 8 0.21574362930233804 1107 9 0.27047471348042507 1107 10-11 0.29233214096182536 1107 12-13 0.23818346917418154 1107 14-15 0.3846854927768675 1107 16-17 0.40596308672278525 1107 18-19 0.2349996958577094 1107 20-21 0.46071866499838166 1107 22-23 0.4219740963605787 1107 24-25 0.35494388978252545 1107 26-27 0.39060180372080566 1107 28-29 0.5794161911342712 1107 30-31 0.6572544351639067 1107 32-33 0.5904037522766075 1107 34-35 0.778657927725547 1107 36-37 0.9910598057684723 1107 38-39 0.8989015441896839 1107 40-41 1.0611498475443426 1107 42-43 1.0388879415920727 1107 44-45 0.7092447421474262 1107 46-47 0.9505935466728204 1107 48-49 1.0035062905591872 1107 50-51 0.9082650365153384 1107 52-53 1.0237664451562054 1107 54-55 1.135184088825902 1107 56-57 1.3902179284388367 1107 58-59 1.503487956138141 1107 60-61 1.4416316270144982 1107 62-63 1.6515536943529092 1107 64-65 1.8656044807825332 1107 66-67 2.2589726789046516 1107 68-69 2.242213088245716 1107 70-71 2.5422899472450915 1107 72-73 2.5493729368770772 1107 74-75 2.7524196116270545 1107 76-77 2.9939075402133675 1107 78-79 2.953038049838728 1107 80-81 3.205038351680173 1107 82-83 3.425370755575802 1107 84-85 3.402905370991867 1107 86-87 3.7510394447544293 1107 88-89 3.631227561184243 1107 90-91 3.736255392968083 1107 92-93 3.6361889586134453 1107 94-95 3.8235011877121536 1107 96-97 3.6686209673526733 1107 98-99 3.75096186873947 1107 100-101 3.26482296310094 1108 1 0.4572451399815769 1108 2 0.3757864827658466 1108 3 0.43857154005912946 1108 4 0.29562422854862547 1108 5 0.18069168536604963 1108 6 0.15362219051547044 1108 7 0.30592676294205035 1108 8 0.2340103681721004 1108 9 0.4247914272080422 1108 10-11 0.4824358039796621 1108 12-13 0.4755949881468311 1108 14-15 0.5476772152075355 1108 16-17 0.6200600967409642 1108 18-19 0.6516795782698068 1108 20-21 0.6180069874367788 1108 22-23 0.6358757712145575 1108 24-25 0.667360243783996 1108 26-27 0.7437358693998348 1108 28-29 0.6048639148026993 1108 30-31 0.8121059030786455 1108 32-33 0.8169026112098123 1108 34-35 0.9497986077239915 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2311 40-41 0.7546322335873157 2311 42-43 0.7855033790578219 2311 44-45 0.7125892264883049 2311 46-47 0.9229379574006913 2311 48-49 1.0803333245275013 2311 50-51 1.1783752924432278 2311 52-53 0.9884984529101075 2311 54-55 1.3703621096174814 2311 56-57 1.4924852090925214 2311 58-59 1.492317591099411 2311 60-61 1.574739077468859 2311 62-63 1.8246019272317504 2311 64-65 2.113499148746971 2311 66-67 2.273200028590974 2311 68-69 2.596810220610081 2311 70-71 2.9272593978466723 2311 72-73 3.079476567634231 2311 74-75 3.345773174905613 2311 76-77 3.463534404053842 2311 78-79 3.7287801098578655 2311 80-81 3.818267427182583 2311 82-83 4.107603847076767 2311 84-85 4.479270278852923 2311 86-87 4.4389513708796855 2311 88-89 4.424588894724781 2311 90-91 4.761989273764083 2311 92-93 5.057586215302006 2311 94-95 5.15055110551096 2311 96-97 4.929797057847601 2311 98-99 4.831554208079083 2311 100-101 4.557633425963175 2312 1 -0.0321084114428416 2312 2 -0.0025496000713829403 2312 3 0.0068581318262630475 2312 4 -1.9576480039518174E-4 2312 5 0.01336752863780788 2312 6 -0.14093118354963963 2312 7 0.05341308606200812 2312 8 -0.006000129933674714 2312 9 -0.012583459497186311 2312 10-11 -0.05104891508189979 2312 12-13 0.12036451886194044 2312 14-15 0.17048467603525097 2312 16-17 0.25595514176544754 2312 18-19 0.26340371282814345 2312 20-21 0.20530020409551497 2312 22-23 0.17967276001875376 2312 24-25 0.05477784017420362 2312 26-27 0.18627259646864758 2312 28-29 0.32096514001641197 2312 30-31 0.4513370309403655 2312 32-33 0.4324429050263561 2312 34-35 0.5042827986199825 2312 36-37 0.5616976269433707 2312 38-39 0.7896329569620022 2312 40-41 0.9988780664438082 2312 42-43 0.8577405308536612 2312 44-45 1.1293335667704412 2312 46-47 1.0792287619017173 2312 48-49 1.3914402838536404 2312 50-51 1.5244377916676441 2312 52-53 1.593122744712879 2312 54-55 1.809934219917693 2312 56-57 1.9802088711874717 2312 58-59 2.136639879612307 2312 60-61 2.2112127688637706 2312 62-63 2.341384943459108 2312 64-65 2.5077604244051095 2312 66-67 2.7687664278122206 2312 68-69 3.1557989652895557 2312 70-71 3.5824954038417864 2312 72-73 3.8705637270825335 2312 74-75 3.365071161688231 2312 76-77 3.3532007568087963 2312 78-79 3.728886968530034 2312 80-81 4.20425577338262 2312 82-83 4.6840943187302635 2312 84-85 5.0709308901948305 2312 86-87 4.875729988666205 2312 88-89 4.754671541290069 2312 90-91 5.288171716677422 2312 92-93 5.495540967893152 2312 94-95 5.49025551374142 2312 96-97 5.57393217133151 2312 98-99 5.324240593732718 2312 100-101 4.592229093430021 2313 1 -0.3034235091857127 2313 2 -0.10394689631749898 2313 3 -0.1794158405619264 2313 4 -0.03994418360816354 2313 5 -0.026752858627041576 2313 6 -0.05540454147205054 2313 7 -0.06709947342031342 2313 8 -0.0015338981438191013 2313 9 0.016849034101042548 2313 10-11 0.050933742051931574 2313 12-13 0.05652168010309566 2313 14-15 -0.0754784873411225 2313 16-17 0.09221136407990116 2313 18-19 -0.06743677926655067 2313 20-21 -0.08278871333271098 2313 22-23 0.0032944537412262775 2313 24-25 0.05920752769460336 2313 26-27 0.09071716272732289 2313 28-29 0.247705626107404 2313 30-31 0.40533823690125814 2313 32-33 0.5310341234384097 2313 34-35 0.4629342628705544 2313 36-37 0.6009396465885288 2313 38-39 0.548199325270815 2313 40-41 0.704800456856681 2313 42-43 0.6561081766457306 2313 44-45 0.7230591767334502 2313 46-47 1.045269999499503 2313 48-49 1.1138049852217051 2313 50-51 1.2159127966924785 2313 52-53 1.3200548271880734 2313 54-55 1.5405951230310038 2313 56-57 1.7070417620741125 2313 58-59 1.7605960839156616 2313 60-61 2.0522771729180924 2313 62-63 2.0309152712873093 2313 64-65 2.3773006452891146 2313 66-67 2.5107371297493266 2313 68-69 2.4483286640342605 2313 70-71 2.8595968951090107 2313 72-73 3.2340441249974248 2313 74-75 3.3428796540543964 2313 76-77 3.4511635443012594 2313 78-79 3.6297541118383876 2313 80-81 3.7953900030946777 2313 82-83 4.037361898481606 2313 84-85 4.300405409702947 2313 86-87 4.450282256107613 2313 88-89 4.504292394494346 2313 90-91 4.385722728562143 2313 92-93 4.606802606590403 2313 94-95 4.8173763212653675 2313 96-97 4.7976124664003805 2313 98-99 4.384179068844766 2313 100-101 4.2232402994692215 2314 1 -0.3160259927399345 2314 2 -0.2709842617321989 2314 3 -0.2298538001137267 2314 4 -0.20430258178193128 2314 5 -0.26771596199976244 2314 6 -0.17493896422658395 2314 7 -0.20192044248544505 2314 8 -0.11712184419249638 2314 9 -0.1408469776390291 2314 10-11 -0.23426607858592519 2314 12-13 -0.2616684182913147 2314 14-15 0.02087070323624829 2314 16-17 -0.005153107948267177 2314 18-19 0.002665411295531328 2314 20-21 0.0036751772971470587 2314 22-23 -0.0172958871997011 2314 24-25 -0.007456463826457593 2314 26-27 0.1228042603726891 2314 28-29 0.1754473955760929 2314 30-31 0.09724171764784728 2314 32-33 0.1150757334174628 2314 34-35 0.24765510505734767 2314 36-37 0.16808452737894441 2314 38-39 0.5428095438174694 2314 40-41 0.5331682103967879 2314 42-43 0.7270893436206691 2314 44-45 0.8257682075154591 2314 46-47 0.8532021883800667 2314 48-49 0.7080427215899263 2314 50-51 0.6423813242413843 2314 52-53 0.9890189343773415 2314 54-55 1.1627562839932395 2314 56-57 1.340269108686357 2314 58-59 1.733440808273759 2314 60-61 1.7707136394838692 2314 62-63 1.8597588185813372 2314 64-65 2.149411360648532 2314 66-67 2.434948980899126 2314 68-69 2.602183931013787 2314 70-71 2.824347393195648 2314 72-73 3.0136630823530552 2314 74-75 2.9743751623575356 2314 76-77 3.345522044385337 2314 78-79 3.3506077635395286 2314 80-81 3.6773855088500618 2314 82-83 4.010744836657718 2314 84-85 4.186666498818415 2314 86-87 4.303986186278667 2314 88-89 4.507735052111382 2314 90-91 4.433935221746889 2314 92-93 4.652331673958212 2314 94-95 4.826674363058782 2314 96-97 4.9317060506368975 2314 98-99 4.7947349386237725 2314 100-101 4.439888877056088 2315 1 -0.36570192526064105 2315 2 -0.29014850340412224 2315 3 -0.42690921209150545 2315 4 -0.26623660835851837 2315 5 -0.26028326832238236 2315 6 -0.35902150222947427 2315 7 -0.17872697877896826 2315 8 -0.21035865256025943 2315 9 -0.357008126170804 2315 10-11 -0.31684478068803656 2315 12-13 -0.2648365976120388 2315 14-15 -0.458145037184174 2315 16-17 -0.3015781908729096 2315 18-19 -0.27624883546379664 2315 20-21 -0.32224157246374574 2315 22-23 -0.20667441022528266 2315 24-25 -0.3699618055980949 2315 26-27 -0.22868812887457324 2315 28-29 -0.20813250715278997 2315 30-31 -0.22146364040347066 2315 32-33 -0.2812430228145999 2315 34-35 0.03421571635951892 2315 36-37 -0.0133640084668869 2315 38-39 0.0794477379934122 2315 40-41 0.2879083967991818 2315 42-43 0.282009115298095 2315 44-45 0.5625562256673078 2315 46-47 0.7984299513478561 2315 48-49 0.7547399804979662 2315 50-51 0.7621400287854527 2315 52-53 1.050116083397505 2315 54-55 1.2070395708616388 2315 56-57 1.4345094070115891 2315 58-59 1.7701489542211313 2315 60-61 1.941622351150297 2315 62-63 1.9264470219145942 2315 64-65 2.037983276156968 2315 66-67 2.4732265902273873 2315 68-69 2.7563487322998874 2315 70-71 2.870848787143675 2315 72-73 3.054439877697064 2315 74-75 3.3527264881343832 2315 76-77 3.5962775235168145 2315 78-79 3.997183433550312 2315 80-81 4.327429514873543 2315 82-83 4.432922855013064 2315 84-85 4.623973177559179 2315 86-87 4.9711124148861465 2315 88-89 5.107150509672277 2315 90-91 4.871899447139128 2315 92-93 5.248122357908478 2315 94-95 5.448235279605047 2315 96-97 5.48838860695443 2315 98-99 5.325249173173333 2315 100-101 5.031167035823714 2316 1 -0.7755358451396894 2316 2 -0.6584438772695513 2316 3 -0.539896304680596 2316 4 -0.6580023237825472 2316 5 -0.39836498290873124 2316 6 -0.4894862098845323 2316 7 -0.6306804569354583 2316 8 -0.34917333646404813 2316 9 -0.42102099271930626 2316 10-11 -0.360851032635388 2316 12-13 -0.33967640423072964 2316 14-15 -0.4600397117917723 2316 16-17 -0.4985273018602996 2316 18-19 -0.4969172932657031 2316 20-21 -0.5103069420896986 2316 22-23 -0.583495340968625 2316 24-25 -0.6507937640818966 2316 26-27 -0.5673777355228893 2316 28-29 -0.5707671615334888 2316 30-31 -0.6131693391760678 2316 32-33 -0.3370329696424008 2316 34-35 -0.45334742881948387 2316 36-37 -0.3052521195513265 2316 38-39 -0.22875979447942996 2316 40-41 -0.19122400504166848 2316 42-43 0.09759933437163681 2316 44-45 0.15395009324247866 2316 46-47 0.29375344109905654 2316 48-49 0.2831712729246334 2316 50-51 0.007641014590873851 2316 52-53 0.18465043079942234 2316 54-55 0.2395207103096908 2316 56-57 0.4727211789585297 2316 58-59 0.7289245379149882 2316 60-61 0.7994211957659125 2316 62-63 1.0720078328289482 2316 64-65 1.310251050024437 2316 66-67 1.489202512057613 2316 68-69 1.6627984153608857 2316 70-71 1.9708933526997363 2316 72-73 2.3110611828421526 2316 74-75 2.4531148724861254 2316 76-77 2.8153006312045648 2316 78-79 3.2847506649694083 2316 80-81 3.212715028130841 2316 82-83 3.5422648937404055 2316 84-85 3.673577897677273 2316 86-87 4.104196147053539 2316 88-89 4.194818237058765 2316 90-91 4.575191168927471 2316 92-93 4.495742761526845 2316 94-95 4.4306433775113625 2316 96-97 4.605429696652507 2316 98-99 4.654472430008848 2316 100-101 4.253395926562764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3070.0 3 895.0 4 1042.0 5 1169.0 6 1405.0 7 1786.0 8 2317.0 9 2880.0 10 3709.0 11 4784.0 12 6263.0 13 8156.0 14 9743.0 15 12380.0 16 15467.0 17 20381.0 18 19910.0 19 16157.0 20 16073.0 21 17203.0 22 19165.0 23 21281.0 24 23744.0 25 26213.0 26 28479.0 27 31242.0 28 33882.0 29 37316.0 30 41433.0 31 46371.0 32 52785.0 33 61332.0 34 75941.0 35 98875.0 36 112912.0 37 125148.0 38 72197.0 39 5412.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.52823279513142 18.485401901485407 14.60573126392744 21.380634039455728 2 15.802062215165572 20.403651074232698 40.39432121069355 23.399965499908184 3 19.35957255363951 25.910225132881276 29.681177703774473 25.04902460970474 4 12.62315487972515 17.151896738192807 36.49029694447897 33.73465143760308 5 13.118487861945225 37.98603012735054 33.27664856650626 15.618833444197975 6 30.80955062486142 36.11115491980381 17.587035760571993 15.492258694762779 7 27.92856546619798 34.43159368942394 19.96058208664521 17.679258757732867 8 27.14938505528057 33.70217959668975 19.380798292010866 19.767637056018817 9 27.613906704322545 16.75760653320163 20.07902168922773 35.5494650732481 10-11 25.19474316346925 27.042797586752176 26.06428217006812 21.698177079710447 12-13 26.676643068935483 24.88367304729779 27.12572029036988 21.31396359339684 14-15 23.32270666929435 25.624001576610517 26.65589316145187 24.397398592643256 16-17 21.389806944722036 29.17443607000277 27.179517619175037 22.256239366100157 18-19 21.3169068913246 26.542726416551087 31.101685444579076 21.038681247545238 20-21 22.327157341926018 25.430039981393648 31.516477890117716 20.726324786562618 22-23 22.031520832888948 25.47444009901036 31.489254556600336 21.004784511500354 24-25 21.625253123988315 25.811875190741624 31.555366321314178 21.007505363955886 26-27 21.08822241762482 26.143893606995906 31.47861640120358 21.289267574175692 28-29 21.05260717198946 26.409224189941 31.541904928406705 20.996263709662834 30-31 21.14922538591573 26.0287955626358 31.455152151783082 21.36682689966539 32-33 20.95642402760651 26.17475373358939 31.362382744911084 21.50643949389302 34-35 21.633536270919823 26.347846895277527 30.98512842202114 21.03348841178151 36-37 21.654860259284938 25.926174979073096 31.006508789300263 21.412455972341704 38-39 21.24649465109723 26.318725239995842 30.995352167010402 21.439427941896525 40-41 21.434068991097924 26.132603857566767 31.131583827893177 21.30174332344214 42-43 21.341041041319507 26.231105126942577 30.74028121264724 21.687572619090673 44-45 21.338568183725446 26.489097178401423 30.690175892642298 21.48215874523084 46-47 21.702833031946955 26.8365094820791 29.90462280335698 21.55603468261696 48-49 21.74748638579718 26.84922487718818 29.657332427651728 21.74595630936291 50-51 21.78704692808712 27.18890483820715 29.46094086141155 21.56310737229418 52-53 22.025581480525055 27.109192559311733 29.296724867648827 21.568501092514385 54-55 21.49389855198111 27.198350260808446 29.516580611391934 21.791170575818505 56-57 21.69301155082614 27.46402015483318 29.167945507028136 21.675022787312543 58-59 21.706056725764892 26.928650971221717 29.27856339482998 22.08672890818341 60-61 21.520458021566775 27.123934074788743 29.638759551195243 21.716848352449247 62-63 21.51792627347709 27.253916518691263 29.547867531492102 21.680289676339545 64-65 21.36290402969124 27.119036193734885 29.442779218912634 22.075280557661237 66-67 21.294953088700073 27.28073945394014 29.30063520454643 22.123672252813357 68-69 21.47290688811701 27.041742623318676 29.493836616082476 21.991513872481843 70-71 21.408034185418618 27.28045369124903 29.15715564779486 22.154356475537497 72-73 21.551668579244474 27.33465475072736 28.967231166904288 22.146445503123875 74-75 21.46164217413559 27.702183927657398 28.78269585988442 22.053478038322588 76-77 21.61459951207616 27.272411161605532 28.864515363295556 22.248473963022754 78-79 21.743994503219668 27.321446205872412 28.800328619930742 22.134230670977175 80-81 21.873920588711208 27.708919547839972 28.470169650420267 21.946990213028556 82-83 21.935953197958668 27.564633079395723 28.400814343649866 22.09859937899574 84-85 21.887307684283485 27.380843650828606 28.453940429359253 22.277908235528653 86-87 21.92700147888532 27.36865041283617 28.350926969026858 22.353421139251655 88-89 21.922724690771243 27.334732156312675 28.325006086039206 22.41753706687688 90-91 21.9606955778236 27.353960048278513 28.29523340101537 22.390110972882518 92-93 22.13501942241836 26.998877032770057 28.30874273108227 22.557360813729318 94-95 21.949827270408758 26.84704736732281 28.40578702093622 22.79733834133222 96-97 22.174648879986275 26.666620298886713 28.274423068897885 22.88430775222913 98-99 22.051503662306455 26.709822084748886 27.89264714951215 23.34602710343251 100-101 22.829214726363727 25.712610901637973 28.19087612990181 23.267298242096487 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2771.0 1 1767.5 2 1410.0 3 1788.5 4 1127.5 5 875.5 6 1599.5 7 3233.0 8 4984.0 9 5525.0 10 4252.5 11 2853.0 12 2578.0 13 2597.5 14 2669.5 15 2777.0 16 2833.5 17 2903.5 18 3045.0 19 3214.5 20 3415.5 21 3784.5 22 4257.0 23 4859.0 24 5641.0 25 6574.0 26 7836.0 27 9174.5 28 10326.0 29 11656.5 30 13214.5 31 15145.0 32 17517.0 33 18933.0 34 19848.5 35 20953.5 36 22433.0 37 24147.5 38 25178.0 39 25677.5 40 26653.0 41 28589.0 42 30041.0 43 31114.0 44 32669.0 45 34681.5 46 35993.5 47 36926.0 48 38319.5 49 38552.5 50 38009.5 51 36996.0 52 35730.5 53 35304.0 54 35098.5 55 33440.5 56 30525.0 57 27893.5 58 25722.0 59 22993.5 60 19472.5 61 16537.5 62 13935.0 63 11117.0 64 8769.5 65 7067.0 66 5488.5 67 4264.0 68 3268.5 69 2332.5 70 1761.5 71 1327.5 72 1053.0 73 826.5 74 613.5 75 478.0 76 340.0 77 221.5 78 142.0 79 87.5 80 58.5 81 39.5 82 20.0 83 14.0 84 10.5 85 6.5 86 5.0 87 3.5 88 4.0 89 3.5 90 1.5 91 1.0 92 1.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38942326414580003 2 0.024107154447120957 3 0.045247274500750105 4 0.0374588092178341 5 0.05813533014748016 6 0.05553917505317482 7 0.030412102533291054 8 0.026981469015816148 9 0.03087570165727415 10-11 0.028094106913375577 12-13 0.02846498621256205 14-15 0.023689915235536173 16-17 0.028140466825773886 18-19 0.02378263506033279 20-21 0.036299811407876366 22-23 0.02290179672476491 24-25 0.04533999432554672 26-27 0.037273369568240865 28-29 0.0286967857745536 30-31 0.037134289831045934 32-33 0.03398181578796089 34-35 0.03282281797800315 36-37 0.03342549683918117 38-39 0.015391490916238766 40-41 0.01094093932600105 42-43 0.010848219501204431 44-45 0.009689221691246691 46-47 0.015576930565832002 48-49 0.013027135383924979 50-51 0.009457422129255145 52-53 0.00394059255385631 54-55 0.004126032203449549 56-57 0.006212228261373477 58-59 0.002874314568695191 60-61 0.00496051062661912 62-63 0.0067685472101531915 64-65 0.00792754502011093 66-67 0.0043114718530427865 68-69 0.004821430889424191 70-71 0.015159691354247218 72-73 0.012099937135958787 74-75 0.008715663530882193 76-77 0.006583107560559954 78-79 0.006351307998568406 80-81 0.008762023443280502 82-83 0.009596501866450073 84-85 0.004682351152229263 86-87 2.781594743898572E-4 88-89 0.021371919615620693 90-91 0.01775584644855255 92-93 0.011589978099577382 94-95 0.02253091742557843 96-97 0.01696772793778129 98-99 0.015530570653433693 100-101 0.11237642765350231 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1078518.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.196333693352656 #Duplication Level Percentage of deduplicated Percentage of total 1 82.94687958827664 51.58991801694797 2 10.3950783328183 12.930715215130139 3 2.8325079655835266 5.2851483184953745 4 1.212210214443173 3.0158012401599263 5 0.6425049774852415 1.9980726989656055 6 0.39107027683430273 1.4593882461322858 7 0.2721549443240526 1.1848927813432208 8 0.19463822931582825 0.9684627408008437 9 0.15129304058530904 0.8468885193953245 >10 0.8566941478111831 9.751571459059395 >50 0.07113860239794659 3.03787091539762 >100 0.030237149219346254 3.6620332394578776 >500 0.0023950206034234747 1.0080038547311427 >1k 7.484439385698359E-4 0.9053135202184935 >5k 2.9937757542793434E-4 1.3431672312003504 >10k+ 1.4968878771396717E-4 1.0127520025644563 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10878 1.0086062541376222 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8813 0.8171398159326039 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5614 0.5205290964082194 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2619 0.24283322114234535 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2208 0.2047253731509349 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2003 0.18571780906762797 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1644 0.15243139196564173 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1250 0.11589978099577385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.271982479661907E-5 0.0 12-13 0.0 0.0 0.0 9.271982479661907E-5 1.8543964959323814E-4 14-15 0.0 0.0 0.0 9.271982479661907E-5 2.3179956199154768E-4 16-17 0.0 0.0 0.0 9.271982479661907E-5 2.781594743898572E-4 18-19 0.0 0.0 0.0 9.271982479661907E-5 2.781594743898572E-4 20-21 0.0 0.0 0.0 1.390797371949286E-4 2.781594743898572E-4 22-23 0.0 0.0 0.0 2.781594743898572E-4 2.781594743898572E-4 24-25 0.0 0.0 0.0 0.001390797371949286 2.781594743898572E-4 26-27 0.0 0.0 0.0 0.0026425150067036435 2.781594743898572E-4 28-29 0.0 0.0 0.0 0.007973904932509239 2.781594743898572E-4 30-31 0.0 0.0 0.0 0.021696439002408863 2.781594743898572E-4 32-33 0.0 0.0 0.0 0.04659171196030108 3.708792991864763E-4 34-35 0.0 0.0 0.0 0.07751377352997355 3.708792991864763E-4 36-37 0.0 0.0 0.0 0.1307349529632329 3.708792991864763E-4 38-39 0.0 0.0 0.0 0.2126529181710458 3.708792991864763E-4 40-41 0.0 0.0 0.0 0.30115399093941875 3.708792991864763E-4 42-43 0.0 0.0 0.0 0.3909995011673426 4.172392115847858E-4 44-45 0.0 0.0 0.0 0.4949847846767509 5.099590363814049E-4 46-47 0.0 0.0 0.0 0.6123217229568723 5.563189487797144E-4 48-49 0.0 0.0 0.0 0.7300759004485786 5.563189487797144E-4 50-51 0.0 0.0 0.0 0.8656322843012356 5.563189487797144E-4 52-53 0.0 0.0 0.0 0.997340795424833 6.490387735763334E-4 54-55 0.0 0.0 0.0 1.13317533875188 7.417585983729526E-4 56-57 0.0 0.0 0.0 1.2856067307175216 7.417585983729526E-4 58-59 0.0 0.0 0.0 1.4487009025347746 7.417585983729526E-4 60-61 0.0 0.0 0.0 1.6300608798369614 7.417585983729526E-4 62-63 0.0 0.0 0.0 1.8021952345718848 7.417585983729526E-4 64-65 0.0 0.0 0.0 1.9771575439631048 7.417585983729526E-4 66-67 9.271982479661907E-5 0.0 0.0 2.1673259046209705 7.881185107712621E-4 68-69 9.271982479661907E-5 0.0 0.0 2.3789125448068553 8.344784231695716E-4 70-71 9.271982479661907E-5 0.0 0.0 2.6049171177486143 8.344784231695716E-4 72-73 1.390797371949286E-4 0.0 0.0 2.8347232035070347 8.344784231695716E-4 74-75 1.8543964959323814E-4 0.0 0.0 3.0593369790768445 8.344784231695716E-4 76-77 1.8543964959323814E-4 0.0 0.0 3.2882158665872985 8.344784231695716E-4 78-79 1.8543964959323814E-4 0.0 0.0 3.5514010892725016 8.344784231695716E-4 80-81 1.8543964959323814E-4 0.0 0.0 3.8281234063780114 8.344784231695716E-4 82-83 1.8543964959323814E-4 0.0 0.0 4.103872165323157 8.344784231695716E-4 84-85 1.8543964959323814E-4 0.0 0.0 4.3796672841807 8.344784231695716E-4 86-87 1.8543964959323814E-4 0.0 0.0 4.663065428671565 8.808383355678811E-4 88-89 1.8543964959323814E-4 0.0 0.0 4.958099911174408 9.271982479661906E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4355 0.0 51.066563 1 GTATCAA 7435 0.0 41.40164 1 TATCAAC 9315 0.0 32.966408 2 ATCAACG 9530 0.0 32.322433 3 TCAACGC 9690 0.0 31.78873 4 CAACGCA 9770 0.0 31.528435 5 AACGCAG 9985 0.0 30.793371 6 TATAACG 65 0.005822362 29.252876 2 GTGGTAT 2055 0.0 28.564861 1 TGGTATC 2035 0.0 27.563828 2 ACGCAGA 11305 0.0 27.022188 7 CGCAGAG 11470 0.0 26.592047 8 GCAGAGT 12025 0.0 25.08583 9 GAGTACT 7225 0.0 24.392467 12-13 GTACATG 6875 0.0 23.115877 1 GTACTTT 7630 0.0 23.037596 14-15 TACATGG 6910 0.0 22.564087 2 GTCTTAG 325 0.0 22.026564 1 CAGAGTA 11775 0.0 21.744911 10-11 CCGTATA 110 0.0028646549 21.60724 2 >>END_MODULE