FastQCFastQC Report
Thu 26 May 2016
SRR938171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938171_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078518
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104110.9653060959576011No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91460.8480155175898779No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56080.5199727774594397No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25880.23995890657365015No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23990.22243485968708915No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18130.16810104235627038No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17230.15975625812457464No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.11061475098236655No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48950.043.15991
GTATCAA77250.036.9575271
TATCAAC98550.028.8731822
ATCAACG98850.028.730753
TCAACGC101000.028.0707514
CAACGCA102000.027.7009855
AACGCAG105350.026.9594276
GTACATG69050.024.2565541
GTAAGGT4750.024.0351454
ACGCAGA117650.023.8986747
CGCAGAG118000.023.7875398
TACATGG68950.023.6016272
GAGTACT71650.022.9021312-13
GCAGAGT123250.022.504539
ACATGGG70350.021.9770073
TAAGGTG5450.021.8198855
AGGTAAG5050.021.6713812
GTACTTT75350.021.65147414-15
AGTACTT73050.021.58548712-13
GTGGTAT27400.020.4918691
Produced by FastQC (version 0.11.5)