FastQCFastQC Report
Thu 26 May 2016
SRR938170_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938170_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070736
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106620.9957636616308783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87770.8197165314325847No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55580.5190822014016527No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27320.25515159665874687No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22290.20817456403819432No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20030.18706758715500366No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17270.16129092512066467No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12980.12122502652381166No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49800.045.3542061
GTATCAA80050.037.4614071
TATAACG600.00394031131.683922
CCGTATA600.003951145331.6661519
TATCAAC98150.030.4572352
ATCAACG99750.030.0163443
TCAACGC100650.029.7951624
CAACGCA102300.029.221685
AACGCAG103850.028.8730136
GTACATG69600.027.5119191
ATAACGC1056.8798385E-527.1576463
TACATGG69550.026.7866922
ACGCAGA115850.025.7073947
ACATGGG68650.025.6840523
TAACGCA1309.977972E-625.5908574
CGCAGAG116300.025.5670838
GCAGAGT120850.024.3293559
GTGTACG1000.001603538523.875681
GAGTACT71250.023.66738912-13
TAAGGTG5050.022.5865555
Produced by FastQC (version 0.11.5)