##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938170_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1070736 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89695592564367 33.0 31.0 34.0 30.0 34.0 2 32.055244243212144 34.0 31.0 34.0 30.0 34.0 3 32.11211447079392 34.0 31.0 34.0 30.0 34.0 4 35.649010587110176 37.0 35.0 37.0 33.0 37.0 5 35.667720147636764 37.0 35.0 37.0 35.0 37.0 6 35.71776422946459 37.0 35.0 37.0 35.0 37.0 7 35.66666199698152 37.0 35.0 37.0 33.0 37.0 8 35.64555595403535 37.0 35.0 37.0 33.0 37.0 9 37.26826687437426 39.0 37.0 39.0 34.0 39.0 10-11 37.19741514248143 39.0 37.0 39.0 34.0 39.0 12-13 37.0854827894084 39.0 37.0 39.0 33.5 39.0 14-15 38.228795426697154 40.0 38.0 41.0 33.5 41.0 16-17 38.08827479416028 40.0 38.0 41.0 33.0 41.0 18-19 38.27317798224773 40.0 38.0 41.0 33.5 41.0 20-21 38.27972441386112 40.0 38.0 41.0 34.0 41.0 22-23 38.17987020143154 40.0 38.0 41.0 34.0 41.0 24-25 38.06022679726842 40.0 38.0 41.0 33.0 41.0 26-27 37.872850543924926 40.0 38.0 41.0 33.0 41.0 28-29 37.65771301235785 40.0 37.5 41.0 32.5 41.0 30-31 37.39251131931681 40.0 37.0 41.0 32.0 41.0 32-33 37.07849227073714 40.0 36.0 41.0 31.5 41.0 34-35 36.81431510661825 39.0 35.0 41.0 31.0 41.0 36-37 36.53941354358123 39.0 35.0 41.0 30.0 41.0 38-39 36.240298262129976 39.0 35.0 40.5 30.0 41.0 40-41 35.99485494090047 39.0 35.0 40.0 30.0 41.0 42-43 35.7541429446661 39.0 35.0 40.0 28.5 41.0 44-45 35.39892279702933 39.0 35.0 40.0 26.5 41.0 46-47 34.88460367448185 38.0 34.5 40.0 24.5 41.0 48-49 34.38266949089224 38.0 34.0 40.0 22.5 41.0 50-51 33.630963187938015 37.5 33.5 39.5 20.0 40.5 52-53 33.739567923372334 38.0 33.5 40.0 20.0 40.5 54-55 33.84239859311725 38.0 34.0 40.0 19.5 41.0 56-57 33.54512877123773 38.0 33.5 40.0 17.5 41.0 58-59 33.2336411589785 37.5 33.0 40.0 13.5 41.0 60-61 32.824338118826674 37.0 33.0 40.0 9.0 41.0 62-63 32.29028630773599 36.0 32.0 39.5 7.5 41.0 64-65 31.786328749570387 35.5 31.0 39.0 2.0 40.5 66-67 31.26461844936567 35.0 31.0 39.0 2.0 40.0 68-69 30.683078275130377 35.0 30.0 38.0 2.0 40.0 70-71 30.16736011491161 35.0 29.5 37.5 2.0 40.0 72-73 29.55652747269168 34.5 29.0 37.0 2.0 39.0 74-75 28.89786791515369 34.0 28.0 36.0 2.0 39.0 76-77 28.257647076403522 34.0 26.5 35.5 2.0 38.5 78-79 27.56141523213939 33.5 26.0 35.0 2.0 37.0 80-81 26.93262391476517 33.0 25.0 35.0 2.0 37.0 82-83 26.239487137819218 32.5 23.0 35.0 2.0 36.0 84-85 25.602075114687466 32.0 20.0 35.0 2.0 36.0 86-87 24.9371693862913 31.5 18.0 34.5 2.0 35.0 88-89 24.159613107245857 31.0 9.5 34.0 2.0 35.0 90-91 23.37472308767054 31.0 3.5 34.0 2.0 35.0 92-93 22.511923574064944 30.0 2.0 34.0 2.0 35.0 94-95 21.59187091869518 29.0 2.0 34.0 2.0 35.0 96-97 20.434153236652172 29.0 2.0 34.0 2.0 35.0 98-99 18.998426783072578 26.0 2.0 33.5 2.0 35.0 100-101 15.385112670163327 11.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22995971764330392 1101 2 0.2245780360500973 1101 3 0.16287038605189963 1101 4 0.06199318330769188 1101 5 0.2202252052284095 1101 6 0.22987518276857344 1101 7 0.16438758627314343 1101 8 0.1428426065904702 1101 9 0.31279468072926164 1101 10-11 0.3050905119381184 1101 12-13 0.38658280321944716 1101 14-15 0.5549739748794948 1101 16-17 0.6660997914961442 1101 18-19 0.4977479142161201 1101 20-21 0.5994573261508975 1101 22-23 0.5450043473402459 1101 24-25 0.5031443370899993 1101 26-27 0.5738893353461876 1101 28-29 0.4088493202568273 1101 30-31 0.5696302854434023 1101 32-33 0.3350966289391266 1101 34-35 0.6200318687456914 1101 36-37 0.638124426305815 1101 38-39 0.643066724868504 1101 40-41 0.7713633955604564 1101 42-43 0.8307599384127968 1101 44-45 0.9199284011755609 1101 46-47 1.1092677804271531 1101 48-49 1.2640956598467596 1101 50-51 1.1408008586928062 1101 52-53 1.1559303490350672 1101 54-55 1.2395434703584627 1101 56-57 1.280484357115185 1101 58-59 1.3549770662372893 1101 60-61 1.280888043639166 1101 62-63 1.19723441807718 1101 64-65 1.0950003965606463 1101 66-67 1.158191255107976 1101 68-69 1.4212111684496271 1101 70-71 1.4901938817197866 1101 72-73 1.650103980963614 1101 74-75 1.613923542978302 1101 76-77 1.8144195999958619 1101 78-79 2.072695768333002 1101 80-81 2.056374633990604 1101 82-83 2.344202499107194 1101 84-85 2.4140543154969727 1101 86-87 2.640796744105927 1101 88-89 2.9253032930395158 1101 90-91 3.3785662393763083 1101 92-93 3.5077491395075135 1101 94-95 3.542554877848712 1101 96-97 3.8985087597225423 1101 98-99 4.051799484735778 1101 100-101 4.1963497121392095 1102 1 0.4275246919028248 1102 2 0.3357819582601209 1102 3 0.2878343649610642 1102 4 0.29399432040590767 1102 5 0.3244634273052469 1102 6 0.40171915108559375 1102 7 0.1398406731832722 1102 8 0.29511446652489326 1102 9 0.4531250622802361 1102 10-11 0.4361464361419536 1102 12-13 0.45180385589201677 1102 14-15 0.3798301825790489 1102 16-17 0.5041436126134613 1102 18-19 0.49421151928477514 1102 20-21 0.7531856064066744 1102 22-23 0.6944139908677514 1102 24-25 0.665647160364685 1102 26-27 0.7002386659380235 1102 28-29 0.6648750646278003 1102 30-31 0.5600243367062845 1102 32-33 0.6551357458966933 1102 34-35 0.5479166713501087 1102 36-37 0.6879011928969376 1102 38-39 0.43820261199982724 1102 40-41 0.6826215441153352 1102 42-43 0.6768150448405237 1102 44-45 0.5951155161393089 1102 46-47 0.33815650056858004 1102 48-49 0.42795268142826615 1102 50-51 0.31572998476273284 1102 52-53 0.22108860818442366 1102 54-55 0.21256659395168498 1102 56-57 0.25285569367289185 1102 58-59 0.18520869158679432 1102 60-61 0.32137331588072726 1102 62-63 0.17128852370270664 1102 64-65 0.0912152613939341 1102 66-67 0.1312932356838452 1102 68-69 0.29348768191956864 1102 70-71 0.6510689411059118 1102 72-73 0.6113959114604306 1102 74-75 0.678077592527746 1102 76-77 0.8007447283088283 1102 78-79 1.0993820421556002 1102 80-81 1.2273268369236945 1102 82-83 1.3647861186621668 1102 84-85 1.430966704415713 1102 86-87 1.5462355394048828 1102 88-89 2.0716511438459975 1102 90-91 2.1562615905137967 1102 92-93 2.4618597300976326 1102 94-95 2.4735034202601405 1102 96-97 2.7488430743872136 1102 98-99 3.0753748019160874 1102 100-101 3.1793054081445646 1103 1 0.5328169718726627 1103 2 0.6000360542928647 1103 3 0.49540137537130136 1103 4 0.34146227194883494 1103 5 0.3494448201032867 1103 6 0.5020841950083792 1103 7 0.5208427199754908 1103 8 0.4289675773552659 1103 9 0.7454172736972211 1103 10-11 0.714617291387718 1103 12-13 0.7590344676772318 1103 14-15 1.06174679783107 1103 16-17 0.8505203496936957 1103 18-19 0.7920148901263104 1103 20-21 0.8251287614046632 1103 22-23 0.9674189805358537 1103 24-25 0.9506427778789615 1103 26-27 0.893381422350167 1103 28-29 1.097869551020061 1103 30-31 0.9776709418712528 1103 32-33 0.9788802494271565 1103 34-35 0.9647198316364651 1103 36-37 1.1917028156408094 1103 38-39 1.2195606439454565 1103 40-41 1.658530114200829 1103 42-43 1.5832936563515219 1103 44-45 1.5605838545161745 1103 46-47 1.6613844094128822 1103 48-49 1.8283692624038679 1103 50-51 1.6597355277502643 1103 52-53 1.8242471807182383 1103 54-55 1.8743421372330218 1103 56-57 2.0462080259387605 1103 58-59 1.9739741037363174 1103 60-61 2.1186521350089293 1103 62-63 1.8678754487156866 1103 64-65 2.0091171035069273 1103 66-67 1.929396525241284 1103 68-69 2.1674446992830276 1103 70-71 2.1607618243408204 1103 72-73 2.311034050348507 1103 74-75 2.322048981506409 1103 76-77 2.427993506599119 1103 78-79 2.6642312014038687 1103 80-81 2.9933045474543896 1103 82-83 3.301996605289464 1103 84-85 3.4488247216714463 1103 86-87 3.499809958419487 1103 88-89 3.5701539985825086 1103 90-91 3.747378120564427 1103 92-93 4.099830296442104 1103 94-95 4.952408117109648 1103 96-97 5.221182611753413 1103 98-99 5.366677281570578 1103 100-101 4.645802039261682 1104 1 0.1884104832533211 1104 2 0.21128518658616713 1104 3 0.16913909047122644 1104 4 0.16224887090393736 1104 5 0.1965777345017372 1104 6 0.1898513573334739 1104 7 0.2558176934876002 1104 8 0.20310352416027655 1104 9 0.3708328595362502 1104 10-11 0.33740920618805603 1104 12-13 0.25082322219843434 1104 14-15 0.4387904754460692 1104 16-17 0.5252627778110437 1104 18-19 0.4208327995925387 1104 20-21 0.42731492559303774 1104 22-23 0.31361796851583534 1104 24-25 0.3666601868154302 1104 26-27 0.4862945420754201 1104 28-29 0.7260526645343646 1104 30-31 0.5916579171370486 1104 32-33 0.4527074753231872 1104 34-35 0.7224812396960871 1104 36-37 0.6660666496256908 1104 38-39 0.7006309373216482 1104 40-41 0.6828624367319662 1104 42-43 0.7455022895764714 1104 44-45 0.8494000285690007 1104 46-47 0.9365479036220776 1104 48-49 0.9716892909011818 1104 50-51 1.0274193902324598 1104 52-53 0.8616499062306389 1104 54-55 0.966869355822034 1104 56-57 0.9476274114197452 1104 58-59 0.9389689888336861 1104 60-61 1.1546368451035605 1104 62-63 1.2393429690839568 1104 64-65 1.3401517752397858 1104 66-67 1.3855316682758847 1104 68-69 1.469132617152308 1104 70-71 1.352954290819941 1104 72-73 1.5944955607061821 1104 74-75 1.9039673440068619 1104 76-77 1.8528181385088054 1104 78-79 2.033465518747043 1104 80-81 2.1788613411411397 1104 82-83 2.5464935180803785 1104 84-85 2.7430777632117618 1104 86-87 2.9107033309670705 1104 88-89 3.123897011260162 1104 90-91 3.1571640253541666 1104 92-93 3.335296209269547 1104 94-95 3.4573426280884227 1104 96-97 3.6360948363707095 1104 98-99 3.799445996633967 1104 100-101 3.9185382381583267 1105 1 0.18907374718522618 1105 2 0.2165172279576808 1105 3 0.09588377762612765 1105 4 0.09497640073397662 1105 5 0.23468005895563238 1105 6 0.21915617592910053 1105 7 0.2812405663294655 1105 8 0.28139223587950823 1105 9 0.21883094718135965 1105 10-11 0.2490132948566739 1105 12-13 0.10691514990603679 1105 14-15 0.3274429386130393 1105 16-17 0.19922310318072078 1105 18-19 0.23789821021014035 1105 20-21 0.2324786204781617 1105 22-23 0.27506785673734413 1105 24-25 0.3218982884665138 1105 26-27 0.334540091136752 1105 28-29 0.355954757350915 1105 30-31 0.3875005653445456 1105 32-33 0.48568718012023027 1105 34-35 0.4622174828614405 1105 36-37 0.5006344843551318 1105 38-39 0.55582392355808 1105 40-41 0.6791993344075138 1105 42-43 0.6908991705525764 1105 44-45 0.48942952192962963 1105 46-47 0.6954868160039069 1105 48-49 0.8858555311037293 1105 50-51 1.0425768228286785 1105 52-53 0.8128646556044075 1105 54-55 0.8749248141138466 1105 56-57 0.968824922664929 1105 58-59 0.8815949753125238 1105 60-61 1.045587164278281 1105 62-63 1.009917618261106 1105 64-65 1.1932926834736897 1105 66-67 1.5857481549297994 1105 68-69 1.6817541594261165 1105 70-71 1.7430999395392028 1105 72-73 1.6155617658937835 1105 74-75 1.773361585394536 1105 76-77 1.848821720807404 1105 78-79 1.899176456878319 1105 80-81 2.059273507489081 1105 82-83 2.1977968148724685 1105 84-85 2.308146648389666 1105 86-87 2.6637183200585675 1105 88-89 2.70009997675697 1105 90-91 2.9889676992303222 1105 92-93 3.116149765980296 1105 94-95 3.342715806637724 1105 96-97 3.5787023053286084 1105 98-99 3.4666221756950293 1105 100-101 3.5500984908046505 1106 1 0.08410604368796015 1106 2 0.08009054948368544 1106 3 0.048588284160310025 1106 4 0.032897606383265554 1106 5 0.1086447476785537 1106 6 -6.505008519255284E-4 1106 7 0.14490097771919608 1106 8 0.05998457066922924 1106 9 0.11354906903554252 1106 10-11 0.09238014015252105 1106 12-13 0.15669148360183982 1106 14-15 0.25968214109778387 1106 16-17 -0.04577207838943309 1106 18-19 0.053128111171879766 1106 20-21 0.04120119937994815 1106 22-23 0.02973571064776337 1106 24-25 0.027143051679757946 1106 26-27 0.03328038102390707 1106 28-29 0.0277765470720297 1106 30-31 0.04683269189995798 1106 32-33 0.07305337102121001 1106 34-35 0.17184075001733135 1106 36-37 0.11153162390075977 1106 38-39 -0.05722767270958684 1106 40-41 0.32986317078926675 1106 42-43 0.3873201841158078 1106 44-45 0.2871026761128732 1106 46-47 0.41521210038893486 1106 48-49 0.49661267572824386 1106 50-51 0.6899353140089985 1106 52-53 0.6243737031831458 1106 54-55 0.3080056139732008 1106 56-57 0.7564737893572087 1106 58-59 1.0114020102200953 1106 60-61 1.1876295111140749 1106 62-63 1.0374282481080215 1106 64-65 1.1533074480543242 1106 66-67 1.253259810320909 1106 68-69 0.8816905105724508 1106 70-71 1.2684825651927838 1106 72-73 1.2538604694757005 1106 74-75 1.094249478763981 1106 76-77 1.4614590297794514 1106 78-79 1.4446170176393736 1106 80-81 1.5777963820080494 1106 82-83 1.8789832067149952 1106 84-85 1.8109616044052146 1106 86-87 1.4156664470567542 1106 88-89 1.7887187966323417 1106 90-91 2.2213405480292963 1106 92-93 2.5664271512658523 1106 94-95 2.8231360518567357 1106 96-97 3.0961467306006263 1106 98-99 3.194950636336923 1106 100-101 3.178390406260739 1107 1 0.128772404401019 1107 2 -0.05997861062673593 1107 3 -0.05050784964409871 1107 4 -0.13680501139649692 1107 5 -0.08180903052279831 1107 6 -0.07367985598041571 1107 7 0.02978918868020486 1107 8 0.0037792763165711563 1107 9 0.027211513594480152 1107 10-11 0.03213829085839137 1107 12-13 -0.006460234729033232 1107 14-15 0.07667366847606871 1107 16-17 0.36193013440573196 1107 18-19 0.1637272147330009 1107 20-21 0.2370714510723957 1107 22-23 0.182314829235537 1107 24-25 0.20098933422609377 1107 26-27 0.4030047334981077 1107 28-29 0.439479741837566 1107 30-31 0.38758852762632756 1107 32-33 0.39207412139980846 1107 34-35 0.36386325715342593 1107 36-37 0.35450674393022297 1107 38-39 0.3121644047597414 1107 40-41 0.1674635667047113 1107 42-43 0.4214323179389794 1107 44-45 0.4949903374519309 1107 46-47 0.4447959122634302 1107 48-49 0.32764849028284004 1107 50-51 0.1922174569247801 1107 52-53 0.2967059844426032 1107 54-55 0.6408060042595096 1107 56-57 0.7233541805191592 1107 58-59 0.6445438228467069 1107 60-61 0.6500683627403774 1107 62-63 0.585299694426844 1107 64-65 0.8295922382246097 1107 66-67 0.8201960236836818 1107 68-69 0.8280570247574666 1107 70-71 0.9997318957563763 1107 72-73 1.1276205950678353 1107 74-75 1.4592402806100253 1107 76-77 1.7378485061218605 1107 78-79 1.681376249607201 1107 80-81 1.7050889818136916 1107 82-83 1.8317125917873263 1107 84-85 1.9885848764774607 1107 86-87 2.058443062089186 1107 88-89 2.281620620704846 1107 90-91 2.7232200518215315 1107 92-93 3.04574635496294 1107 94-95 3.194856008918986 1107 96-97 3.5416290023609243 1107 98-99 3.53307368128943 1107 100-101 3.2483170946673106 1108 1 0.1814672215130102 1108 2 0.0827347087736996 1108 3 0.09344444890942327 1108 4 0.14965386033844652 1108 5 -0.09007663204192795 1108 6 0.13772564376863983 1108 7 0.21238879766018215 1108 8 0.07313064750843523 1108 9 0.10380349693492974 1108 10-11 0.1619928943326272 1108 12-13 0.16042441436420773 1108 14-15 0.23528809426841235 1108 16-17 0.35722890516462513 1108 18-19 0.3363112428385051 1108 20-21 0.27072726356256993 1108 22-23 0.1651436568496436 1108 24-25 0.352765778640574 1108 26-27 0.4025504161726232 1108 28-29 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5 1057.0 6 1210.0 7 1492.0 8 1859.0 9 2378.0 10 2947.0 11 4010.0 12 5232.0 13 6674.0 14 7670.0 15 9924.0 16 12771.0 17 16880.0 18 16041.0 19 11361.0 20 10787.0 21 10989.0 22 11860.0 23 12870.0 24 14479.0 25 16320.0 26 18357.0 27 21143.0 28 24419.0 29 28481.0 30 33773.0 31 40360.0 32 49760.0 33 63009.0 34 84960.0 35 117498.0 36 146301.0 37 165022.0 38 88438.0 39 5530.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.5223901602829 18.43070436093725 14.667813845523048 21.379091633256802 2 15.836592525875778 20.43079268919356 40.28997314786613 23.44264163706454 3 19.361910288432696 25.867835086804536 29.771165700925934 24.999088923836837 4 12.623377011739157 17.20362884008321 36.551181484991034 33.6218126631866 5 13.135856615787855 37.9271020576801 33.32735088993519 15.609690436596848 6 30.711982973202613 36.20222780351883 17.53621793104222 15.549571292236338 7 27.874738961639622 34.371195380496985 20.053819452555256 17.700246205308133 8 27.13369285735642 33.779841988818134 19.357958112835234 19.728507040990223 9 27.627012854988482 16.685883432407127 20.122289641820664 35.564814070783726 10-11 25.194378283827334 27.02388416446538 26.09462468475725 21.687112866950038 12-13 26.685537611702664 24.818173663772644 27.118192724704087 21.378095999820605 14-15 23.374420503659632 25.553522945345623 26.674256254636436 24.397800296358305 16-17 21.436539762386154 29.11554431060966 27.205907084689642 22.242008842314547 18-19 21.268941738750588 26.526773638192058 31.162585350242587 21.041699272814764 20-21 22.33787313929708 25.417929562533025 31.557998049763274 20.686199248406613 22-23 22.011955402005647 25.47136006129538 31.49250977011897 21.024174766580003 24-25 21.69008213872838 25.858699705332334 31.475515855325938 20.975702300613353 26-27 21.060872100466394 26.136475135240705 31.524236797619725 21.27841596667318 28-29 21.043647013589734 26.386431663811432 31.586213211505843 20.983708111092994 30-31 21.143810727803956 26.006613426620213 31.553510544762098 21.296065300813737 32-33 20.894314678457185 26.21411021935765 31.392208372246 21.49936672993917 34-35 21.581896884000926 26.3074978800741 31.128548101877918 20.98205713404706 36-37 21.64920278456613 25.922337308860072 31.082922645178986 21.345537261394814 38-39 21.166973234695966 26.31689574413823 31.083289037444896 21.43284198372091 40-41 21.42465126381107 26.177879394379584 31.126301703219838 21.271167638589503 42-43 21.319471658314505 26.173222050077467 30.808566051818737 21.69874023978929 44-45 21.259022071440516 26.553169389362107 30.74841509515033 21.439393444047045 46-47 21.72042418090887 26.841124603492304 29.907902314890283 21.530548900708542 48-49 21.65788839418336 26.809059027732367 29.836535425420806 21.696517152663468 50-51 21.710187706376598 27.139334270541916 29.55396966993756 21.596508353143932 52-53 21.917044311876175 27.14837428466143 29.40228917682763 21.532292226634762 54-55 21.474455677496916 27.21126784244749 29.51881787976472 21.79545860029088 56-57 21.52761463695088 27.478400413987202 29.297814377368205 21.696170571693717 58-59 21.643174727717685 26.91447626520204 29.346522445777058 22.09582656130322 60-61 21.432858239079657 27.125722577773587 29.716517391139867 21.72490179200689 62-63 21.45535998684792 27.235129881472474 29.64888240003064 21.660627731648972 64-65 21.250150624496168 27.138891198205023 29.55588892739479 22.055069249904022 66-67 21.245511398211427 27.277248830943694 29.349115963193583 22.1281238076513 68-69 21.37308857361426 27.168431563206084 29.485307785066517 21.973172078113134 70-71 21.36232713916812 27.288670641880042 29.261067086512966 22.087935132438872 72-73 21.459949877215056 27.32686575668498 29.038802657647178 22.174381708452778 74-75 21.315432931639126 27.77742750379347 28.86822739413436 22.038912170433044 76-77 21.50561185913587 27.348922531452143 28.94839462019952 22.197070989212474 78-79 21.54707873029454 27.453882431842956 28.930700116479024 22.068338721383476 80-81 21.668867268449908 27.717167169294388 28.64160673977304 21.97235882248266 82-83 21.674414868327275 27.69307220165687 28.560039455580245 22.072473474435604 84-85 21.662575449333353 27.53641463647012 28.592144862720044 22.208865051476483 86-87 21.761379291021253 27.511195998860554 28.496630693147907 22.230794016970286 88-89 21.711224501235 27.528109831060753 28.370844247807455 22.38982141989679 90-91 21.75164704167772 27.510220440132883 28.38610468570745 22.35202783248194 92-93 21.96140905430979 27.104595530198157 28.426798261100245 22.507197154391807 94-95 21.737400084454094 27.078654414594865 28.531517072058776 22.652428428892268 96-97 21.930090063832193 26.823299824468478 28.38491381766581 22.861696294033514 98-99 21.90590735529373 26.92366795502049 28.021205291981456 23.149219397704318 100-101 22.61387685234769 25.989715480330094 28.25651902449063 23.13988864283159 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2812.0 1 1802.0 2 1419.5 3 1784.0 4 1132.0 5 900.0 6 1604.5 7 3177.0 8 4949.0 9 5532.5 10 4240.0 11 2821.5 12 2499.5 13 2539.0 14 2630.0 15 2684.5 16 2740.0 17 2821.0 18 2989.5 19 3162.0 20 3325.0 21 3622.5 22 4147.5 23 4865.5 24 5648.0 25 6498.0 26 7625.5 27 9077.5 28 10264.0 29 11447.5 30 12994.5 31 14861.5 32 17421.0 33 18974.5 34 19888.5 35 20897.0 36 22340.5 37 24217.5 38 25184.0 39 25860.5 40 26728.5 41 28590.5 42 30074.0 43 31339.5 44 33093.5 45 34915.5 46 35954.5 47 36930.0 48 38909.0 49 39089.5 50 38173.5 51 36912.0 52 35400.5 53 35229.5 54 35421.0 55 33609.0 56 30775.5 57 28237.5 58 25462.0 59 22382.0 60 18736.5 61 15623.0 62 13322.5 63 10547.0 64 8024.5 65 6258.0 66 4723.5 67 3730.5 68 2780.0 69 1961.0 70 1535.5 71 1140.0 72 865.0 73 669.0 74 493.5 75 383.0 76 276.0 77 162.5 78 103.0 79 69.5 80 40.5 81 24.0 82 9.0 83 4.0 84 5.5 85 8.5 86 6.0 87 2.0 88 2.5 89 2.5 90 2.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46071113701229804 2 0.04015929229987597 3 0.05351459183216031 4 0.05995875734074506 5 0.08769668713856636 6 0.08340057679950987 7 0.04585630818427699 8 0.04034607970592191 9 0.04576291448125401 10-11 0.04940526889914974 12-13 0.045202552263116216 14-15 0.038291418239416626 16-17 0.03997250489383004 18-19 0.04795766650229375 20-21 0.05692346199249862 22-23 0.04749069798717891 24-25 0.06696328506746761 26-27 0.0566899777349412 28-29 0.04478228059951286 30-31 0.04566952077823105 32-33 0.046463367253926274 34-35 0.049825540562753096 36-37 0.04478228059951286 38-39 0.02643041795549977 40-41 0.024609240746551908 42-43 0.0237686974193452 44-45 0.015596748404835553 46-47 0.02769123294630983 48-49 0.027084173876660542 50-51 0.016904260247157094 52-53 0.01648398858355374 54-55 0.017277835059248965 56-57 0.016017020068438907 58-59 0.014569417671582912 60-61 0.01648398858355374 62-63 0.01709104765320303 64-65 0.018024984683432707 66-67 0.010039823074968993 68-69 0.012608149908100596 70-71 0.01732453191076045 72-73 0.015409960998789617 74-75 0.013588783789841755 76-77 0.009479460856831188 78-79 0.014662811374605879 80-81 0.018258468940990123 82-83 0.015409960998789617 84-85 0.014009055453445107 86-87 0.0038291418239416626 88-89 0.027270961282706476 90-91 0.03100670940362517 92-93 0.016250504325996323 94-95 0.03100670940362517 96-97 0.02549648092527009 98-99 0.023208335201207395 100-101 0.15442648794847658 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1070736.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.998974776381054 #Duplication Level Percentage of deduplicated Percentage of total 1 82.93617316029139 51.419577078144776 2 10.34318022108496 12.825331392692199 3 2.872843648444409 5.3434008268917434 4 1.2248576366186192 3.0375967086950197 5 0.6440777140484413 1.9966078973659245 6 0.4048707859069125 1.5060944185881746 7 0.2676649235649827 1.1616465591239131 8 0.18799971193528112 0.932463151859392 9 0.14906167654656866 0.8317504011902201 >10 0.8658921002889333 9.945553469950328 >50 0.06872854280073776 2.9484257996020116 >100 0.031018451514260047 3.7669539280058193 >500 0.002420951303114224 1.0025508015161235 >1k 7.565472822231949E-4 0.9370712039248207 >5k 3.02618912889278E-4 1.3447708187268301 >10k+ 1.51309456444639E-4 1.0002055437227388 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10662 0.9957636616308783 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8777 0.8197165314325847 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5558 0.5190822014016527 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2732 0.25515159665874687 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2229 0.20817456403819432 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2003 0.18706758715500366 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1727 0.16129092512066467 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1298 0.12122502652381166 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 9.339370302296737E-5 0.0 0.0 0.0 9.339370302296737E-5 7 9.339370302296737E-5 0.0 0.0 0.0 9.339370302296737E-5 8 9.339370302296737E-5 0.0 0.0 0.0 9.339370302296737E-5 9 9.339370302296737E-5 0.0 0.0 0.0 9.339370302296737E-5 10-11 9.339370302296737E-5 0.0 0.0 0.0 9.339370302296737E-5 12-13 9.339370302296737E-5 0.0 0.0 0.0 9.339370302296737E-5 14-15 9.339370302296737E-5 0.0 0.0 0.0 9.339370302296737E-5 16-17 9.339370302296737E-5 0.0 0.0 0.0 9.339370302296737E-5 18-19 1.4009055453445107E-4 0.0 0.0 9.339370302296737E-5 9.339370302296737E-5 20-21 1.8678740604593475E-4 0.0 0.0 3.268779605803858E-4 9.339370302296737E-5 22-23 1.8678740604593475E-4 0.0 0.0 6.070590696492879E-4 9.339370302296737E-5 24-25 2.8018110906890214E-4 0.0 0.0 0.0013075118423215432 9.339370302296737E-5 26-27 2.8018110906890214E-4 0.0 0.0 0.003502263863361277 9.339370302296737E-5 28-29 2.8018110906890214E-4 0.0 0.0 0.008125252162998163 9.339370302296737E-5 30-31 2.8018110906890214E-4 0.0 0.0 0.02026643355598392 9.339370302296737E-5 32-33 2.8018110906890214E-4 0.0 0.0 0.04585630818427698 9.339370302296737E-5 34-35 2.8018110906890214E-4 0.0 0.0 0.07975822238161415 9.339370302296737E-5 36-37 2.8018110906890214E-4 0.0 0.0 0.1345336292045845 9.339370302296737E-5 38-39 2.8018110906890214E-4 0.0 0.0 0.21480551695282496 9.339370302296737E-5 40-41 2.8018110906890214E-4 0.0 0.0 0.30324935371557504 9.339370302296737E-5 42-43 2.8018110906890214E-4 0.0 0.0 0.3891248636451936 9.339370302296737E-5 44-45 2.8018110906890214E-4 0.0 0.0 0.4943795669520778 9.339370302296737E-5 46-47 4.202716636033532E-4 0.0 0.0 0.615464502921355 9.339370302296737E-5 48-49 4.669685151148369E-4 0.0 0.0 0.7419195768144529 9.339370302296737E-5 50-51 4.669685151148369E-4 0.0 0.0 0.873044335858699 1.8678740604593475E-4 52-53 4.669685151148369E-4 0.0 0.0 0.99613723644297 1.8678740604593475E-4 54-55 4.669685151148369E-4 0.0 0.0 1.126094574199429 1.8678740604593475E-4 56-57 4.669685151148369E-4 0.0 0.0 1.2784664006814004 2.3348425755741843E-4 58-59 4.669685151148369E-4 0.0 0.0 1.4424190463382196 2.8018110906890214E-4 60-61 4.669685151148369E-4 0.0 0.0 1.6217816529938287 2.8018110906890214E-4 62-63 4.669685151148369E-4 0.0 0.0 1.7953538500620134 3.735748120918695E-4 64-65 4.669685151148369E-4 0.0 0.0 1.9685057754665949 3.735748120918695E-4 66-67 5.603622181378043E-4 0.0 0.0 2.164258977002735 3.735748120918695E-4 68-69 5.603622181378043E-4 0.0 0.0 2.382006395600783 3.735748120918695E-4 70-71 6.070590696492879E-4 0.0 0.0 2.6220749092213205 3.735748120918695E-4 72-73 6.537559211607717E-4 0.0 0.0 2.847153733506672 4.202716636033532E-4 74-75 6.537559211607717E-4 0.0 0.0 3.0761083964674762 4.669685151148369E-4 76-77 6.537559211607717E-4 0.0 0.0 3.320006051911956 4.669685151148369E-4 78-79 6.537559211607717E-4 0.0 0.0 3.594676932502503 5.136653666263206E-4 80-81 9.339370302296738E-4 0.0 0.0 3.877753246365117 6.070590696492879E-4 82-83 9.339370302296738E-4 0.0 0.0 4.158961686167272 6.537559211607717E-4 84-85 9.339370302296738E-4 0.0 0.0 4.447641622211264 6.537559211607717E-4 86-87 9.339370302296738E-4 0.0 0.0 4.741178030812451 7.004527726722553E-4 88-89 9.339370302296738E-4 0.0 0.0 5.052599333542535 7.938464756952228E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4980 0.0 45.354206 1 GTATCAA 8005 0.0 37.461407 1 TATAACG 60 0.003940311 31.68392 2 CCGTATA 60 0.0039511453 31.666151 9 TATCAAC 9815 0.0 30.457235 2 ATCAACG 9975 0.0 30.016344 3 TCAACGC 10065 0.0 29.795162 4 CAACGCA 10230 0.0 29.22168 5 AACGCAG 10385 0.0 28.873013 6 GTACATG 6960 0.0 27.511919 1 ATAACGC 105 6.8798385E-5 27.157646 3 TACATGG 6955 0.0 26.786692 2 ACGCAGA 11585 0.0 25.707394 7 ACATGGG 6865 0.0 25.684052 3 TAACGCA 130 9.977972E-6 25.590857 4 CGCAGAG 11630 0.0 25.567083 8 GCAGAGT 12085 0.0 24.329355 9 GTGTACG 100 0.0016035385 23.87568 1 GAGTACT 7125 0.0 23.667389 12-13 TAAGGTG 505 0.0 22.586555 5 >>END_MODULE