FastQCFastQC Report
Thu 26 May 2016
SRR938170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938170_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070736
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105800.9881053779829948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90530.8454931934669236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56060.5235650991467551No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26670.249081005962254No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22730.21228388697120487No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18260.17053690171993843No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17860.16680115359901973No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12160.11356674287592834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49850.046.3142551
GTATCAA80000.036.579211
TACCTGG8450.030.358522
TATCAAC100300.029.2705672
ATCAACG101000.028.8768623
TCAACGC102800.028.4609934
CAACGCA103400.028.295845
AACGCAG106150.027.6522796
GTACATG66900.025.3504491
TACATGG66700.025.070352
ACGCAGA119500.024.4438517
CGCAGAG120150.024.2325528
GAGTACT73950.023.7643512-13
GTACCTG11100.023.5387441
ACATGGG68250.023.4547213
GCAGAGT125200.022.8757539
GTACTTT77950.022.42302314-15
AGTACTT74750.021.88973412-13
CATGGGG29800.021.0387574
AATTCGC1501.6734703E-1020.58185678-79
Produced by FastQC (version 0.11.5)