FastQCFastQC Report
Thu 26 May 2016
SRR938169_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938169_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082934
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110141.0170518240262103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91890.8485281651513389No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57320.5293028014634317No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26360.24341280262693757No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21990.20305946622785875No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19790.18274428543198384No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17550.16205973771254756No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.1176433651542938No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48000.047.331121
GTATCAA81200.039.852421
TATCAAC102800.031.473362
ATCAACG103050.031.3062423
TCAACGC103800.031.034274
CAACGCA105350.030.6227665
AACGCAG108100.029.8437426
ACGCAGA121850.026.4870837
CCCTATA2700.026.3946912
CGCAGAG122850.026.2714798
GTACATG70400.025.491551
GCAGAGT127900.024.8625379
GAGTACT75350.024.53669512-13
TACATGG70950.024.2407072
GTGTAAG4400.023.864431
TACGGGA1000.00165146823.7552242
ATTCGCG1900.023.73766980-81
ACATGGG70700.023.0506883
GTACTTT80500.022.86623414-15
AATTCGC2000.022.54765978-79
Produced by FastQC (version 0.11.5)