##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938169_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1082934 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92698354655039 33.0 31.0 34.0 30.0 34.0 2 32.08419349655658 34.0 31.0 34.0 30.0 34.0 3 32.13215671499833 34.0 31.0 34.0 30.0 34.0 4 35.66980443868232 37.0 35.0 37.0 35.0 37.0 5 35.70817058103264 37.0 35.0 37.0 35.0 37.0 6 35.754977681003645 37.0 36.0 37.0 35.0 37.0 7 35.69884868329926 37.0 35.0 37.0 35.0 37.0 8 35.68853503537612 37.0 35.0 37.0 33.0 37.0 9 37.34410499624169 39.0 37.0 39.0 34.0 39.0 10-11 37.26707952654547 39.0 37.0 39.0 34.0 39.0 12-13 37.16236815909372 39.0 37.0 39.0 34.0 39.0 14-15 38.37467195600101 40.0 38.0 41.0 34.0 41.0 16-17 38.30444560795026 40.0 38.0 41.0 33.5 41.0 18-19 38.29924215141458 40.0 38.0 41.0 33.5 41.0 20-21 38.22441857029145 40.0 38.0 41.0 34.0 41.0 22-23 38.070195875279566 40.0 38.0 41.0 33.0 41.0 24-25 38.004943514563216 40.0 38.0 41.0 33.0 41.0 26-27 37.99374615627545 40.0 38.0 41.0 33.0 41.0 28-29 37.888916591408154 40.0 38.0 41.0 33.0 41.0 30-31 37.64912127608885 40.0 37.0 41.0 33.0 41.0 32-33 37.36413484108911 40.0 36.5 41.0 32.5 41.0 34-35 37.131478003276285 40.0 36.0 41.0 31.5 41.0 36-37 36.896420280460305 40.0 35.5 41.0 31.0 41.0 38-39 36.64686444418589 39.0 35.0 41.0 30.5 41.0 40-41 36.40072248170249 39.0 35.0 41.0 30.0 41.0 42-43 36.07639339054827 39.0 35.0 41.0 29.5 41.0 44-45 35.74802019328971 39.0 35.0 40.5 28.5 41.0 46-47 35.43822015007378 39.0 35.0 41.0 26.5 41.0 48-49 35.070890746804515 39.0 35.0 41.0 24.0 41.0 50-51 34.38103106929877 38.5 34.0 40.0 23.5 40.5 52-53 34.42933133505828 38.5 34.5 40.0 23.0 41.0 54-55 34.55256460689202 39.0 35.0 40.0 23.0 41.0 56-57 34.35108418426238 38.0 34.5 40.0 22.0 41.0 58-59 34.09360588918623 38.0 34.0 40.0 21.5 41.0 60-61 33.73033629011556 37.5 34.0 40.0 19.5 41.0 62-63 33.31403575841187 37.0 33.5 40.0 17.5 41.0 64-65 32.90699156181263 36.5 33.0 39.5 13.5 41.0 66-67 32.49078891234369 36.0 32.5 39.0 9.0 41.0 68-69 32.022209571405085 35.0 32.0 39.0 7.5 40.5 70-71 31.54908193851149 35.0 31.5 38.0 4.0 40.0 72-73 31.078295168495956 35.0 31.0 37.5 2.0 39.5 74-75 30.577881939250222 35.0 31.0 37.0 2.0 39.0 76-77 30.065320693597208 35.0 30.0 36.0 2.0 39.0 78-79 29.527666505992055 34.0 29.5 36.0 2.0 38.0 80-81 29.012247745476643 34.0 29.0 35.0 2.0 37.0 82-83 28.501649685022358 34.0 28.5 35.0 2.0 37.0 84-85 28.042891810581253 34.0 27.5 35.0 2.0 36.0 86-87 27.570122463603504 34.0 26.5 35.0 2.0 36.0 88-89 27.04570176945225 33.0 25.5 35.0 2.0 35.5 90-91 26.466654477558187 33.0 24.5 35.0 2.0 35.0 92-93 25.88002685297534 32.5 23.5 35.0 2.0 35.0 94-95 25.331962058629614 32.0 19.5 35.0 2.0 35.0 96-97 24.54993471439626 32.0 12.0 34.5 2.0 35.0 98-99 23.54439005516495 31.5 2.0 34.0 2.0 35.0 100-101 20.77615625698334 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.41337567919753226 1101 2 0.23977814415198395 1101 3 0.2579283065388509 1101 4 0.03642671781464202 1101 5 0.2297084300103407 1101 6 0.22128291140490575 1101 7 0.3145030091131318 1101 8 0.16496442951905976 1101 9 0.12777090675183445 1101 10-11 0.2483254512783617 1101 12-13 0.14525482449889182 1101 14-15 0.1697793814517965 1101 16-17 0.343589573680255 1101 18-19 0.1289853399398595 1101 20-21 0.2456646183533877 1101 22-23 0.34889962143139286 1101 24-25 0.2918580735032634 1101 26-27 0.1820321613126481 1101 28-29 0.3715740072399285 1101 30-31 0.44492246113590994 1101 32-33 0.3400463814480972 1101 34-35 0.3447740758503812 1101 36-37 0.29690694167832277 1101 38-39 0.3539324353493072 1101 40-41 0.1587370756408788 1101 42-43 0.02368114222061024 1101 44-45 0.12389038251416906 1101 46-47 0.3279296766394353 1101 48-49 0.34650113309559316 1101 50-51 0.37814556295526813 1101 52-53 0.35029275163307716 1101 54-55 0.27618310192528384 1101 56-57 0.22517590002150456 1101 58-59 0.23116605433444448 1101 60-61 0.2784472238050313 1101 62-63 0.4037262609052803 1101 64-65 0.4780724613173888 1101 66-67 0.47048920807182526 1101 68-69 0.44332503382588584 1101 70-71 0.7465591059633461 1101 72-73 0.7233217058618386 1101 74-75 0.5469311270535506 1101 76-77 0.4156915426854404 1101 78-79 0.368859355778131 1101 80-81 0.3604925189188215 1101 82-83 0.4110165467215019 1101 84-85 0.5631378496848001 1101 86-87 0.5129662524603624 1101 88-89 0.6797937974196913 1101 90-91 0.7780980025126887 1101 92-93 0.8181800424612895 1101 94-95 0.9476340423865821 1101 96-97 0.8999693678884846 1101 98-99 1.021982186631579 1101 100-101 0.9879548673228449 1102 1 0.2672734337380014 1102 2 0.1839611359483726 1102 3 0.09167904094714174 1102 4 0.18410954838739002 1102 5 0.18452723640152158 1102 6 0.20259086042262453 1102 7 0.10824753196040149 1102 8 0.07774837599676943 1102 9 0.17000259797790562 1102 10-11 0.228795136288916 1102 12-13 0.07412107613832575 1102 14-15 0.2714867694508243 1102 16-17 0.23791588636706962 1102 18-19 0.17833460968579118 1102 20-21 0.24674224506230757 1102 22-23 0.16310619342120702 1102 24-25 0.08811343196374821 1102 26-27 0.18479867981182707 1102 28-29 0.22551280951643804 1102 30-31 0.256503461671997 1102 32-33 0.379822626777063 1102 34-35 0.36626924210854384 1102 36-37 0.39176084141106315 1102 38-39 0.232608185929422 1102 40-41 0.332363559046712 1102 42-43 0.27526024368071944 1102 44-45 0.3077243993170313 1102 46-47 0.06598824730781416 1102 48-49 0.16686882190033714 1102 50-51 0.44529817547057604 1102 52-53 0.5531160445912633 1102 54-55 0.5698118998299719 1102 56-57 0.271382792480388 1102 58-59 0.3005194189629634 1102 60-61 0.2433469585278587 1102 62-63 0.19177157760587704 1102 64-65 0.07316325099669996 1102 66-67 0.04375941691083085 1102 68-69 0.03488868551161062 1102 70-71 -0.15381803865722432 1102 72-73 0.05491273589295531 1102 74-75 -0.02264132368422267 1102 76-77 -0.1628431619390085 1102 78-79 -0.04956791257911064 1102 80-81 0.07358071319429271 1102 82-83 0.16735570190963855 1102 84-85 0.07433151784896452 1102 86-87 0.18283662074262708 1102 88-89 0.39820039354359693 1102 90-91 0.5316567917726225 1102 92-93 0.44706350447680165 1102 94-95 0.4381169022367537 1102 96-97 0.2587265809153543 1102 98-99 0.06967273220049819 1102 100-101 -0.050982619447918154 1103 1 0.32408169738252823 1103 2 0.25781521726273837 1103 3 0.13712755962075107 1103 4 0.05724936510907952 1103 5 0.09032310057296655 1103 6 0.13922181703925673 1103 7 0.13684100876348992 1103 8 0.12769824965549503 1103 9 0.2818525413651898 1103 10-11 0.3844281256216888 1103 12-13 0.21453349526131404 1103 14-15 0.19192181458360125 1103 16-17 0.14970592615336642 1103 18-19 0.2214308627537065 1103 20-21 0.2037604574739902 1103 22-23 0.22890428966950793 1103 24-25 0.27984120252913414 1103 26-27 0.3587518691539415 1103 28-29 0.474639771764771 1103 30-31 0.3527670222542838 1103 32-33 0.5150039734825 1103 34-35 0.6195546398427041 1103 36-37 0.4953368383815402 1103 38-39 0.3736081945143326 1103 40-41 0.4131709413070439 1103 42-43 0.605037198154946 1103 44-45 0.31659976644121457 1103 46-47 0.5300389326221833 1103 48-49 0.4913226546338407 1103 50-51 0.5102792743582611 1103 52-53 0.5891903174254551 1103 54-55 0.3909285868937573 1103 56-57 0.3594453783891183 1103 58-59 0.4234758524475595 1103 60-61 0.24903732368227338 1103 62-63 0.2875562909318461 1103 64-65 0.2908765931883863 1103 66-67 0.3971215065163207 1103 68-69 0.39909924094581584 1103 70-71 0.30433719751844635 1103 72-73 0.428641095955566 1103 74-75 0.16512880292413712 1103 76-77 0.25627505323892663 1103 78-79 0.46054845111968845 1103 80-81 0.2977565855881785 1103 82-83 0.31853884572311486 1103 84-85 0.4570458949993679 1103 86-87 0.33810999769366745 1103 88-89 0.3028892811394499 1103 90-91 0.45204414295557527 1103 92-93 0.3969104545917901 1103 94-95 0.5793811353037306 1103 96-97 0.6409954965743268 1103 98-99 0.6560989386905014 1103 100-101 0.4262673664823673 1104 1 0.38824978419853906 1104 2 0.33688118577515525 1104 3 0.1785567743738241 1104 4 0.26788856476294143 1104 5 0.25005855544753786 1104 6 0.1770228077589806 1104 7 0.3370670553326178 1104 8 0.18015606459752576 1104 9 0.3455738330094107 1104 10-11 0.32530635186251544 1104 12-13 0.18600085537292443 1104 14-15 0.3796486337728382 1104 16-17 0.5925439584886192 1104 18-19 0.5438372874210415 1104 20-21 0.4150204021985431 1104 22-23 0.48121022957290904 1104 24-25 0.5128417941353902 1104 26-27 0.4630471954645259 1104 28-29 0.4581970913469391 1104 30-31 0.3456302199302428 1104 32-33 0.47108338999979793 1104 34-35 0.4735550460201239 1104 36-37 0.5847532673755538 1104 38-39 0.6654497676571793 1104 40-41 0.5923075493723147 1104 42-43 0.717024730273863 1104 44-45 0.6224473969257147 1104 46-47 0.7677326395210144 1104 48-49 0.9455296815033591 1104 50-51 0.813088521075727 1104 52-53 0.8206840790699488 1104 54-55 0.9816730394813646 1104 56-57 0.7887148771131862 1104 58-59 0.9858218802972019 1104 60-61 0.8592306111587646 1104 62-63 0.8760879595263873 1104 64-65 0.8463295251713348 1104 66-67 0.9970040682596988 1104 68-69 1.1280720535479105 1104 70-71 1.1723405705571004 1104 72-73 1.4199312390889638 1104 74-75 1.3998830214299325 1104 76-77 1.427388332591864 1104 78-79 1.5703673489660694 1104 80-81 1.60603995615444 1104 82-83 1.4074603563499508 1104 84-85 1.4825229327297151 1104 86-87 1.247321395385523 1104 88-89 1.3674423535185056 1104 90-91 1.467677558996055 1104 92-93 1.1078026321443062 1104 94-95 1.1504458499640862 1104 96-97 1.2892577635741738 1104 98-99 1.5783437753806844 1104 100-101 1.4143195691119246 1105 1 0.29079773700675204 1105 2 0.30074146041587824 1105 3 0.25068065701663045 1105 4 0.2248601168277915 1105 5 0.25284364445770535 1105 6 0.24490935238179645 1105 7 0.22062477108872258 1105 8 0.2343517776459052 1105 9 0.2871853500924715 1105 10-11 0.32658369190068726 1105 12-13 0.29336511171548096 1105 14-15 0.4751905572669841 1105 16-17 0.2883188136421282 1105 18-19 0.34413707130755 1105 20-21 0.43045864167247316 1105 22-23 0.41837249968953927 1105 24-25 0.5655293377575177 1105 26-27 0.3544737126737161 1105 28-29 0.5715292754386283 1105 30-31 0.6911952684086202 1105 32-33 0.6550190941345448 1105 34-35 0.6237825117741238 1105 36-37 0.6819370647502367 1105 38-39 0.4740661367823833 1105 40-41 0.477295650516794 1105 42-43 0.469740444331336 1105 44-45 0.4887559767153178 1105 46-47 0.6983496981912012 1105 48-49 0.6682686887267622 1105 50-51 0.5072370591562674 1105 52-53 0.46574836652612106 1105 54-55 0.47111038759227597 1105 56-57 0.506297433306905 1105 58-59 0.42574527353124125 1105 60-61 0.4929897245048167 1105 62-63 0.39793308099562097 1105 64-65 0.4107355494399485 1105 66-67 0.7669718614634817 1105 68-69 1.046376950158212 1105 70-71 0.9814984847676094 1105 72-73 0.8700031367672132 1105 74-75 0.8137668415091461 1105 76-77 0.8481263979977314 1105 78-79 0.8269335831254523 1105 80-81 0.7466432239249379 1105 82-83 0.8484281579628146 1105 84-85 0.9142184565736784 1105 86-87 1.0710425837574356 1105 88-89 1.0251690414321217 1105 90-91 0.6790188921724152 1105 92-93 0.9735242577886538 1105 94-95 1.0244117427146158 1105 96-97 0.8814747347898795 1105 98-99 0.9127323087521368 1105 100-101 1.05340920155081 1106 1 -0.0853633049280802 1106 2 0.11060688378463368 1106 3 0.06787880265852664 1106 4 0.08829230072823435 1106 5 0.06837405904108351 1106 6 0.03366176380839647 1106 7 0.30936527015062154 1106 8 0.19305806174918416 1106 9 0.18525521611145734 1106 10-11 -0.5780470744283477 1106 12-13 -0.20576876010085954 1106 14-15 -0.04205507238422257 1106 16-17 0.07280262771658386 1106 18-19 0.15781486500524977 1106 20-21 0.169692014823994 1106 22-23 0.12109530227187548 1106 24-25 0.15063780645613178 1106 26-27 0.19665183892492877 1106 28-29 -0.008303422017085893 1106 30-31 0.15854215315980014 1106 32-33 0.176073785124494 1106 34-35 -0.03396573694281102 1106 36-37 -0.010016215617191904 1106 38-39 -0.0014200366020915567 1106 40-41 0.30069773915140274 1106 42-43 0.3035149763573912 1106 44-45 0.052562821030640805 1106 46-47 0.21489671728738102 1106 48-49 -0.00875856411648357 1106 50-51 0.16795223120721658 1106 52-53 0.1625364951928887 1106 54-55 -0.16402132676245884 1106 56-57 -0.13873436608887602 1106 58-59 0.15839913330285782 1106 60-61 0.053058650770594795 1106 62-63 0.3318591647726379 1106 64-65 0.16474604555684635 1106 66-67 0.1365595943750293 1106 68-69 -0.27971534750531646 1106 70-71 0.1396177540102137 1106 72-73 0.4885340609350841 1106 74-75 0.5373834354331812 1106 76-77 0.7057765351553762 1106 78-79 0.7196883836429784 1106 80-81 0.5172917363683958 1106 82-83 0.5353716271743174 1106 84-85 0.3937473887294516 1106 86-87 0.5539429632986135 1106 88-89 0.2893166290128235 1106 90-91 0.21164967173741545 1106 92-93 0.40702761806819865 1106 94-95 0.3272889924143243 1106 96-97 0.6619625295795437 1106 98-99 0.6927138280418959 1106 100-101 0.1755489931506098 1107 1 0.19779138387065487 1107 2 0.019265561070724857 1107 3 0.024647445580356475 1107 4 -0.0438457269864827 1107 5 0.12707863804786967 1107 6 0.06340793969883407 1107 7 -0.09235852554950696 1107 8 0.041355976322357435 1107 9 0.10336096375124271 1107 10-11 0.12266440224241393 1107 12-13 0.08415022956504714 1107 14-15 0.045010877223141676 1107 16-17 0.04115835364609666 1107 18-19 0.08413174815435553 1107 20-21 0.1709403555388178 1107 22-23 0.3698345556865874 1107 24-25 0.3050893429231891 1107 26-27 0.3104943091818555 1107 28-29 0.4506889731843202 1107 30-31 0.31886786928867394 1107 32-33 0.2922733707684202 1107 34-35 0.40350712521556176 1107 36-37 0.42413187325846025 1107 38-39 0.4204617478583188 1107 40-41 0.3192228052126609 1107 42-43 0.4921539903388066 1107 44-45 0.5456062291713764 1107 46-47 0.35273059027608866 1107 48-49 0.3107453251239036 1107 50-51 0.19443566316721927 1107 52-53 0.16400038790692406 1107 54-55 0.22886793843782982 1107 56-57 0.3620053339371765 1107 58-59 0.2876047233880925 1107 60-61 0.11989062566111386 1107 62-63 0.37406393607041366 1107 64-65 0.2907075005173354 1107 66-67 0.18650562883853894 1107 68-69 0.2985424340769285 1107 70-71 0.19429507731960527 1107 72-73 0.24347469884494544 1107 74-75 0.22376303025744804 1107 76-77 0.25588229520102956 1107 78-79 -0.058826509604504196 1107 80-81 0.13393017967977272 1107 82-83 0.21681988849156753 1107 84-85 0.07531195508224897 1107 86-87 0.2651963283909744 1107 88-89 0.3639255339466345 1107 90-91 0.5883229135154231 1107 92-93 0.4619003338372991 1107 94-95 0.5312554593464753 1107 96-97 0.6406709544061719 1107 98-99 0.49030584858601856 1107 100-101 0.44633192763521023 1108 1 0.22047271507230803 1108 2 0.13571354607540798 1108 3 0.24004428847871395 1108 4 0.1536201384599778 1108 5 0.11390260849828593 1108 6 0.14587757235039334 1108 7 0.18801801119452932 1108 8 0.15883764565363379 1108 9 0.16245554816419627 1108 10-11 0.18501624082227153 1108 12-13 0.07040452509769324 1108 14-15 0.23487112310218095 1108 16-17 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-0.3857770708839823 2316 58-59 -0.36892194700278225 2316 60-61 -0.3130250041528484 2316 62-63 -0.36929174249678454 2316 64-65 -0.32471113405345164 2316 66-67 -0.5867706564356148 2316 68-69 -0.3834696526632051 2316 70-71 -0.5478692786467398 2316 72-73 -0.3837302001782952 2316 74-75 -0.46748339365872127 2316 76-77 -0.3392542307922355 2316 78-79 -0.6094672422573275 2316 80-81 -0.7145962198597822 2316 82-83 -0.7643199723585674 2316 84-85 -0.6879068589275334 2316 86-87 -0.7432233388004654 2316 88-89 -0.7161237271371093 2316 90-91 -0.7089279455911033 2316 92-93 -0.7979497668855373 2316 94-95 -0.7386436485369892 2316 96-97 -0.7818295957605734 2316 98-99 -0.9157531800734375 2316 100-101 -0.9509604055796252 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2979.0 3 782.0 4 811.0 5 911.0 6 1004.0 7 1274.0 8 1616.0 9 1982.0 10 2626.0 11 3198.0 12 4298.0 13 5651.0 14 6627.0 15 8455.0 16 11086.0 17 16246.0 18 14300.0 19 9442.0 20 8615.0 21 8522.0 22 8730.0 23 9457.0 24 10409.0 25 11540.0 26 13037.0 27 15279.0 28 17722.0 29 20996.0 30 24958.0 31 30784.0 32 38450.0 33 49785.0 34 70091.0 35 108322.0 36 152852.0 37 209084.0 38 161000.0 39 20003.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.491235445382664 18.44356655057309 14.592433594195878 21.472764409848374 2 15.813596825528808 20.394406796103052 40.39209708190709 23.399899296461054 3 19.307147958669844 25.75715438012619 29.85515227167103 25.080545389532933 4 12.626534702503111 17.229695078510424 36.500762788841016 33.64300743014545 5 13.147502774797355 37.87494420370016 33.36355344107218 15.613999580430308 6 30.75840086969142 36.09884974805017 17.576955395960113 15.565793986298292 7 27.9779186865056 34.31594685551968 20.053096429251273 17.65303802872345 8 27.189048659076043 33.657154366086836 19.39027409764336 19.763522877193758 9 27.56016304629007 16.68406640108205 20.09484549035839 35.66092506226949 10-11 25.186485787158542 27.030689757143385 26.06356041843788 21.719264037260196 12-13 26.674737020181432 24.90633120619892 27.044928665655334 21.37400310796432 14-15 23.367414065185173 25.568818070610494 26.64187144055613 24.421896423648196 16-17 21.39082347560342 29.16081670743736 27.233945240223694 22.21441457673553 18-19 21.289824233313347 26.554693902818755 31.067313937985023 21.088167925882875 20-21 22.303935385198294 25.421470190046254 31.51537974595811 20.759214678797345 22-23 22.00871344948186 25.48582331933453 31.46391495377621 21.0415482774074 24-25 21.64624438801328 25.871449400271267 31.46649906765347 21.01580714406198 26-27 21.036560574531237 26.126173993073394 31.5031639136429 21.334101518752473 28-29 21.052981741872827 26.450536544437597 31.54126351525546 20.95521819843412 30-31 21.148695258834387 26.007959159062587 31.50803100680636 21.335314575296664 32-33 20.876475026602343 26.22512859917118 31.37801294932317 21.520383424903304 34-35 21.61625057294516 26.29568775597711 31.037455827776384 21.050605843301348 36-37 21.673667154413774 25.886018722281236 30.960328926668367 21.479985196636626 38-39 21.1898896837212 26.290452881974097 31.081310159970844 21.438347274333854 40-41 21.506081140381266 26.12520728535861 31.126015640517537 21.242695933742592 42-43 21.27239142574853 26.18470567639762 30.7951649624679 21.747737935385945 44-45 21.315174418040815 26.449604484342622 30.727010840024548 21.50821025759201 46-47 21.649339319354148 26.880888130655478 29.952241455672674 21.5175310943177 48-49 21.685437158646604 26.859784286359535 29.777238765645798 21.677539789348067 50-51 21.768912004677347 27.210897548688862 29.488193721883626 21.531996724750165 52-53 21.963611038886967 27.167166899041117 29.312570341569653 21.556651720502263 54-55 21.458283206185484 27.19783201336334 29.51498780553699 21.82889697491418 56-57 21.659342866973166 27.47891184423513 29.244336274398876 21.617409014392823 58-59 21.67606139841267 26.924103559715117 29.31844399967119 22.08139104220102 60-61 21.493278602137675 27.159601505415775 29.674416198783604 21.672703693662946 62-63 21.430507688734075 27.313281682576434 29.589076774661606 21.667133854027888 64-65 21.202374102114838 27.179977888264617 29.584098023151746 22.0335499864688 66-67 21.168660489249245 27.346709015193564 29.38792586729666 22.09670462826053 68-69 21.325835660770494 27.181141109190055 29.537809770802852 21.9552134592366 70-71 21.33482128216415 27.30747929858856 29.25704023512794 22.100659184119348 72-73 21.451180595745782 27.33649230433833 29.13802030529535 22.074306794620544 74-75 21.35240126530422 27.789721920347173 28.844465402501697 22.013411411846917 76-77 21.50366266026159 27.37551065270259 28.99084958385788 22.12997710317794 78-79 21.51334777167714 27.51742067761703 28.9492179743527 22.020013576353133 80-81 21.603002743956782 27.83218178274992 28.698915624483373 21.86589984880992 82-83 21.65905448902969 27.780630740882707 28.595957310230492 21.964357459857116 84-85 21.593946435597296 27.68837960200751 28.596912999868852 22.120760962526344 86-87 21.712274590438334 27.645914495705483 28.55062338704576 22.091187526810423 88-89 21.61751439647953 27.699856670326504 28.426805558955703 22.255823374238258 90-91 21.62875697669799 27.6916588211055 28.51444953725818 22.165134664938332 92-93 21.81650063436977 27.34223775726384 28.574582440589317 22.266679167777063 94-95 21.656126281361303 27.251569508798028 28.60354138467864 22.48876282516203 96-97 21.845625276569876 27.057656793757168 28.55609968802456 22.540618241648396 98-99 21.765783924040228 27.11470727609131 28.270102801381526 22.849405998486937 100-101 22.11166047666841 26.578773316559438 28.612777451447442 22.69678875532471 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2817.0 1 1770.0 2 1399.5 3 1811.0 4 1132.5 5 891.5 6 1677.5 7 3420.5 8 5265.5 9 5811.0 10 4396.0 11 2899.5 12 2641.5 13 2672.5 14 2726.5 15 2728.0 16 2777.0 17 2864.0 18 2950.0 19 3108.0 20 3397.5 21 3839.0 22 4271.5 23 4788.0 24 5531.0 25 6528.5 26 7730.5 27 9060.0 28 10322.5 29 11564.5 30 13092.5 31 14917.5 32 17458.5 33 19027.5 34 19768.5 35 20907.5 36 22438.5 37 24420.5 38 25640.0 39 26035.0 40 26937.5 41 29220.5 42 30795.0 43 31830.5 44 33457.5 45 35626.5 46 36668.5 47 37581.5 48 39808.0 49 39990.5 50 39019.0 51 37550.5 52 36067.0 53 35900.0 54 36129.0 55 34147.5 56 30688.0 57 28228.5 58 25707.5 59 22402.5 60 18620.0 61 15564.0 62 13267.0 63 10335.0 64 7723.5 65 6181.0 66 4656.5 67 3580.5 68 2731.5 69 1909.0 70 1408.5 71 1062.0 72 839.5 73 661.0 74 487.5 75 369.5 76 269.5 77 158.5 78 96.0 79 56.5 80 31.5 81 19.5 82 11.0 83 9.5 84 10.0 85 8.5 86 4.5 87 3.5 88 3.0 89 2.0 90 1.0 91 2.5 92 2.5 93 1.0 94 0.5 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4944899689177734 2 0.05069561025879694 3 0.057713581806462806 4 0.0665783879719355 5 0.08061433106726726 6 0.10813216687258873 7 0.06944098162953605 8 0.050418585066125905 9 0.05004921814256455 10-11 0.07516616894473717 12-13 0.11053305187573759 14-15 0.05558972199598498 16-17 0.061084054983960245 18-19 0.0597450998860503 20-21 0.07655129490809227 22-23 0.06454686989234801 24-25 0.0910951175233209 26-27 0.06740946354994858 28-29 0.0675018052808389 30-31 0.07244208788347212 32-33 0.07318082173059484 34-35 0.07442743509761444 36-37 0.06902544384052953 38-39 0.042061658420550097 40-41 0.04515510640537651 42-43 0.042523367075001796 44-45 0.021561794162894506 46-47 0.02774869013254732 48-49 0.02724081061265045 50-51 0.024793754744056422 52-53 0.024978438205837106 54-55 0.024978438205837106 56-57 0.02553248859117915 58-59 0.021700306759230018 60-61 0.02857976571056038 62-63 0.019714959545087698 64-65 0.023177774453475467 66-67 0.015975119444028907 68-69 0.01828366271628742 70-71 0.04243102534411146 72-73 0.0377215970687041 74-75 0.03218109321528367 76-77 0.023177774453475467 78-79 0.015605752520467546 80-81 0.017637270600055037 82-83 0.02206967368279138 84-85 0.0184683461780681 86-87 0.009188002223588879 88-89 0.042892733998563165 90-91 0.053650545647287835 92-93 0.03356621917863877 94-95 0.05438927949441056 96-97 0.04404700563469242 98-99 0.03333536485141292 100-101 0.17443352965185321 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1082934.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.89424991514293 #Duplication Level Percentage of deduplicated Percentage of total 1 82.97504046653219 51.35677891354641 2 10.307507228412584 12.759508567950215 3 2.875447032443229 5.339209117313919 4 1.2087111782090072 2.992490869571806 5 0.6591489797289254 2.0398765841326796 6 0.39991337839168006 1.485140315195026 7 0.26536928043204305 1.1497382794003765 8 0.19957225727432068 0.9881900134292805 9 0.13627137534710565 0.7590973105812604 >10 0.8689042870516219 9.997599313827319 >50 0.06887420670494582 2.9424352851924875 >100 0.031805891964689734 3.910677565067492 >500 0.0022398505484461737 0.9725628066608263 >1k 7.466168494820579E-4 0.9097154524339555 >5k 2.9864673979282315E-4 1.379037312381088 >10k+ 1.4932336989641158E-4 1.0179422933158169 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11014 1.0170518240262103 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9189 0.8485281651513389 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5732 0.5293028014634317 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2636 0.24341280262693757 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2199 0.20305946622785875 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1979 0.18274428543198384 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1755 0.16205973771254756 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1274 0.1176433651542938 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.23417308903405E-5 0.0 12-13 0.0 0.0 0.0 9.23417308903405E-5 2.770251926710215E-4 14-15 0.0 0.0 0.0 9.23417308903405E-5 3.2319605811619175E-4 16-17 0.0 0.0 0.0 2.770251926710215E-4 3.69366923561362E-4 18-19 0.0 0.0 0.0 2.770251926710215E-4 3.69366923561362E-4 20-21 0.0 0.0 0.0 3.2319605811619175E-4 3.69366923561362E-4 22-23 0.0 0.0 0.0 5.078795198968728E-4 3.69366923561362E-4 24-25 0.0 0.0 0.0 0.0011081007706840859 3.69366923561362E-4 26-27 0.0 0.0 0.0 0.001708322021471299 3.69366923561362E-4 28-29 0.0 0.0 0.0 0.006140725104207644 3.69366923561362E-4 30-31 0.0 0.0 0.0 0.01791429579272606 3.69366923561362E-4 32-33 0.0 0.0 0.0 0.041230582842537035 3.69366923561362E-4 34-35 0.0 0.0 0.0 0.07599724452275022 3.69366923561362E-4 36-37 0.0 0.0 0.0 0.13029418228627046 3.69366923561362E-4 38-39 0.0 0.0 0.0 0.21566411249439024 4.617086544517025E-4 40-41 0.0 0.0 0.0 0.30283470645487165 4.617086544517025E-4 42-43 0.0 0.0 0.0 0.38760441541220425 4.617086544517025E-4 44-45 0.0 0.0 0.0 0.4872411430428817 4.617086544517025E-4 46-47 0.0 0.0 0.0 0.6055309003134078 4.617086544517025E-4 48-49 0.0 0.0 0.0 0.7298228700918061 4.617086544517025E-4 50-51 0.0 0.0 0.0 0.8619177161304382 4.617086544517025E-4 52-53 0.0 0.0 0.0 0.9867637362941786 4.617086544517025E-4 54-55 9.23417308903405E-5 0.0 0.0 1.12679997118938 4.617086544517025E-4 56-57 9.23417308903405E-5 0.0 0.0 1.2850275270699785 5.078795198968728E-4 58-59 9.23417308903405E-5 0.0 0.0 1.4559520709479987 5.54050385342043E-4 60-61 9.23417308903405E-5 0.0 0.0 1.6392965776307697 5.54050385342043E-4 62-63 9.23417308903405E-5 0.0 0.0 1.8149305497841972 5.54050385342043E-4 64-65 9.23417308903405E-5 0.0 0.0 1.9891793959742698 5.54050385342043E-4 66-67 9.23417308903405E-5 0.0 0.0 2.1876679465230566 5.54050385342043E-4 68-69 9.23417308903405E-5 0.0 0.0 2.4054559188279248 5.54050385342043E-4 70-71 9.23417308903405E-5 0.0 0.0 2.6317855012401496 6.002212507872132E-4 72-73 9.23417308903405E-5 0.0 0.0 2.867164573279627 6.925629816775538E-4 74-75 9.23417308903405E-5 0.0 0.0 3.1031438665698925 7.38733847122724E-4 76-77 9.23417308903405E-5 0.0 0.0 3.3484958455455276 7.38733847122724E-4 78-79 9.23417308903405E-5 0.0 0.0 3.602574118090299 7.38733847122724E-4 80-81 9.23417308903405E-5 9.23417308903405E-5 0.0 3.8914190523152845 7.38733847122724E-4 82-83 9.23417308903405E-5 9.23417308903405E-5 0.0 4.187512812415161 8.310755780130645E-4 84-85 9.23417308903405E-5 9.23417308903405E-5 0.0 4.483883597707709 8.310755780130645E-4 86-87 9.23417308903405E-5 9.23417308903405E-5 0.0 4.7831169766578565 8.310755780130645E-4 88-89 9.23417308903405E-5 9.23417308903405E-5 0.0 5.09435478062375 8.310755780130645E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4800 0.0 47.33112 1 GTATCAA 8120 0.0 39.85242 1 TATCAAC 10280 0.0 31.47336 2 ATCAACG 10305 0.0 31.306242 3 TCAACGC 10380 0.0 31.03427 4 CAACGCA 10535 0.0 30.622766 5 AACGCAG 10810 0.0 29.843742 6 ACGCAGA 12185 0.0 26.487083 7 CCCTATA 270 0.0 26.394691 2 CGCAGAG 12285 0.0 26.271479 8 GTACATG 7040 0.0 25.49155 1 GCAGAGT 12790 0.0 24.862537 9 GAGTACT 7535 0.0 24.536695 12-13 TACATGG 7095 0.0 24.240707 2 GTGTAAG 440 0.0 23.86443 1 TACGGGA 100 0.001651468 23.755224 2 ATTCGCG 190 0.0 23.737669 80-81 ACATGGG 7070 0.0 23.050688 3 GTACTTT 8050 0.0 22.866234 14-15 AATTCGC 200 0.0 22.547659 78-79 >>END_MODULE