FastQCFastQC Report
Thu 26 May 2016
SRR938169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938169_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082934
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107040.9884258874502048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94040.8683816372927622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58350.5388139997451368No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26830.24775286397878357No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24040.22198952106037856No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19770.18255960197020318No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19070.17609568080787932No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12340.11394969591868019No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47700.044.721651
GTATCAA78050.037.0101241
TATCAAC98750.029.0596052
ATCAACG99900.028.8135493
TCAACGC101200.028.4421024
CAACGCA102600.028.0540035
AACGCAG104900.027.43896
TACCTGG9400.024.2606952
GTACATG69200.024.0298921
TACATGG68650.024.014792
CGCAGAG119700.024.0066078
ACGCAGA120150.023.8771617
GCAGAGT126850.022.503689
GAGTACT75800.022.37013212-13
ACATGGG70150.022.1416343
GTACCTG11150.021.7312761
AGTACTT77900.021.40125312-13
GTGGTAT25500.021.2400021
TGGTATC25500.021.2400022
CTACACG1353.797171E-421.109944
Produced by FastQC (version 0.11.5)