##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938169_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1082934 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.503495134514196 33.0 31.0 34.0 31.0 34.0 2 32.70715943907939 34.0 31.0 34.0 31.0 34.0 3 32.747867367725085 34.0 31.0 34.0 31.0 34.0 4 36.22284552890573 37.0 35.0 37.0 35.0 37.0 5 36.196020256082086 37.0 35.0 37.0 35.0 37.0 6 36.245598531397114 37.0 37.0 37.0 35.0 37.0 7 36.21296127003123 37.0 37.0 37.0 35.0 37.0 8 36.21081709503996 37.0 37.0 37.0 35.0 37.0 9 37.905294320798866 39.0 38.0 39.0 35.0 39.0 10-11 37.90257716536742 39.0 38.0 39.0 35.0 39.0 12-13 37.846714111848 39.0 38.0 39.0 35.0 39.0 14-15 39.122021286615805 40.5 38.5 41.0 36.0 41.0 16-17 39.083059540101246 40.0 38.0 41.0 36.0 41.0 18-19 39.15016242910464 40.0 38.5 41.0 36.0 41.0 20-21 39.10444496155814 40.0 39.0 41.0 35.5 41.0 22-23 38.95085065202497 40.0 38.0 41.0 35.0 41.0 24-25 38.8947345821629 40.0 38.0 41.0 35.0 41.0 26-27 38.83623055514002 40.0 38.0 41.0 35.0 41.0 28-29 38.76282857496395 40.0 38.0 41.0 35.0 41.0 30-31 38.51304234607095 40.0 38.0 41.0 34.5 41.0 32-33 38.269441166313 40.0 37.5 41.0 34.0 41.0 34-35 38.04665519782369 40.0 37.0 41.0 34.0 41.0 36-37 37.88839116695939 40.0 37.0 41.0 33.5 41.0 38-39 37.83482234374394 40.0 37.0 41.0 33.0 41.0 40-41 37.73598344866815 40.0 37.0 41.0 33.0 41.0 42-43 37.53227666690675 40.0 37.0 41.0 33.0 41.0 44-45 37.23940701834091 40.0 36.5 41.0 32.5 41.0 46-47 36.77460999469958 40.0 36.0 41.0 31.0 41.0 48-49 36.31409393370233 40.0 35.5 41.0 30.0 41.0 50-51 35.970560532774854 39.5 35.0 41.0 29.0 41.0 52-53 35.56062373145548 39.0 35.0 41.0 28.0 41.0 54-55 35.33318743339852 39.0 35.0 41.0 27.0 41.0 56-57 35.09152450657196 38.5 35.0 40.0 27.0 41.0 58-59 34.76999567840699 38.0 34.5 40.0 26.0 41.0 60-61 34.424353654054634 38.0 34.0 40.0 26.0 41.0 62-63 34.06457549582892 37.0 34.0 40.0 24.5 41.0 64-65 33.69104072824383 36.5 34.0 40.0 23.0 41.0 66-67 33.27949948935023 36.0 33.0 39.0 22.0 41.0 68-69 32.839091301963 35.5 33.0 39.0 21.5 40.0 70-71 32.33662393091361 35.0 32.5 38.5 19.5 40.0 72-73 31.934752718078848 35.0 32.0 37.5 18.0 39.5 74-75 31.504220940518998 35.0 31.5 37.0 16.5 39.0 76-77 30.207773973298465 33.5 30.0 35.5 13.5 38.0 78-79 30.917101134510506 35.0 31.0 36.0 13.5 38.5 80-81 30.755991131500167 35.0 31.0 36.0 9.5 37.0 82-83 30.40772752540783 35.0 31.0 35.0 7.0 37.0 84-85 30.045998186408404 34.0 31.0 35.0 3.5 36.5 86-87 29.633677583306092 34.0 30.0 35.0 2.0 36.0 88-89 29.192958665994418 34.0 30.0 35.0 2.0 36.0 90-91 28.870016547638173 34.0 29.0 35.0 2.0 35.0 92-93 28.484770540032912 34.0 29.0 35.0 2.0 35.0 94-95 28.106193452232546 34.0 29.0 35.0 2.0 35.0 96-97 27.56780376274085 34.0 28.0 35.0 2.0 35.0 98-99 26.922802774684328 33.0 26.5 35.0 2.0 35.0 100-101 24.59664901092772 31.0 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09204817881882121 1101 2 0.1380605686947476 1101 3 0.1405710510068232 1101 4 0.04574492570862532 1101 5 0.04444593123267282 1101 6 0.13940218172332663 1101 7 0.15349825529926875 1101 8 0.12094474437397196 1101 9 -0.03063413510321311 1101 10-11 0.0657064480282159 1101 12-13 0.13234934985894853 1101 14-15 0.09988169301527705 1101 16-17 0.24759514724198795 1101 18-19 0.2719205138945071 1101 20-21 0.1401987106592344 1101 22-23 0.19903742253993073 1101 24-25 0.21161519867680312 1101 26-27 0.06589221832332726 1101 28-29 0.14648543305066397 1101 30-31 0.13784208886738725 1101 32-33 0.14852354131142675 1101 34-35 0.06758849021741042 1101 36-37 0.2679982860911423 1101 38-39 0.14579054669429325 1101 40-41 0.3229516329130959 1101 42-43 0.3616266005019284 1101 44-45 0.44700148323470756 1101 46-47 0.2165027504737722 1101 48-49 0.26934182149504693 1101 50-51 0.336611915634073 1101 52-53 0.3081028837262707 1101 54-55 0.5021442918482535 1101 56-57 0.48836973841908105 1101 58-59 0.2819405550440379 1101 60-61 0.25998454615778144 1101 62-63 0.1694593594794256 1101 64-65 0.21597492854393607 1101 66-67 0.12632613664002434 1101 68-69 0.30561143780473543 1101 70-71 0.3215581408492767 1101 72-73 0.25828208250167606 1101 74-75 0.5301196929409144 1101 76-77 0.3857035805068456 1101 78-79 0.5988497097284373 1101 80-81 0.6692380462385934 1101 82-83 0.8369432394992309 1101 84-85 0.9021863441040949 1101 86-87 0.9144928608864511 1101 88-89 0.6672505926636063 1101 90-91 0.6338450701326757 1101 92-93 0.8721988716676137 1101 94-95 0.6697774186811465 1101 96-97 0.814908938930639 1101 98-99 0.7800342071598472 1101 100-101 0.7694029275953511 1102 1 -0.024105044961565625 1102 2 -0.03440223612178528 1102 3 0.08969955316998579 1102 4 0.06956052543088731 1102 5 0.030780496987190986 1102 6 -0.04570530399898587 1102 7 -0.022537186206221804 1102 8 0.0086292366381997 1102 9 0.12475676792720236 1102 10-11 0.0029563288414138356 1102 12-13 -0.05387334845107006 1102 14-15 0.06498765552039742 1102 16-17 -0.01845896851065021 1102 18-19 0.1257079835538022 1102 20-21 0.11407899765708862 1102 22-23 0.118092276162713 1102 24-25 0.02895920244330341 1102 26-27 -0.011329453298735359 1102 28-29 0.002241215835930177 1102 30-31 0.03801102750151131 1102 32-33 -0.022375987678167064 1102 34-35 0.026019497380481482 1102 36-37 0.009067683470817656 1102 38-39 -0.0906478747779218 1102 40-41 0.08445538457726087 1102 42-43 0.10747979005937935 1102 44-45 0.12130154515249814 1102 46-47 0.09976694557217769 1102 48-49 -0.033269506888821354 1102 50-51 -0.21624223730879777 1102 52-53 -0.199615079752121 1102 54-55 -0.20364086726859654 1102 56-57 -0.27756123412542166 1102 58-59 -0.3788424379392765 1102 60-61 -0.19745983168711945 1102 62-63 -0.19969183268531054 1102 64-65 -0.2544489412585804 1102 66-67 -0.22312362587656054 1102 68-69 -0.12034042457555927 1102 70-71 0.11708156377547851 1102 72-73 -0.16610650405167604 1102 74-75 -0.0974344158880065 1102 76-77 -0.2536633743841641 1102 78-79 -0.2189087278067987 1102 80-81 -0.007669783295600752 1102 82-83 0.07359184516203854 1102 84-85 0.06691041429869315 1102 86-87 -0.10633614512531508 1102 88-89 -0.2749309510873452 1102 90-91 -0.265195797586788 1102 92-93 -0.17390416507357287 1102 94-95 -0.28274518724673925 1102 96-97 -0.3142227877052868 1102 98-99 -0.3610992840341787 1102 100-101 -0.35762201162432206 1103 1 0.11592439174426517 1103 2 0.21525651366716403 1103 3 0.16515710507769654 1103 4 0.0838458172844696 1103 5 0.05844680340555897 1103 6 0.05792029467932025 1103 7 0.09878477561763077 1103 8 0.037887567101947184 1103 9 0.08695263553261157 1103 10-11 0.057023621189109974 1103 12-13 0.13093975326773233 1103 14-15 0.17829512601453246 1103 16-17 0.22358878076416033 1103 18-19 0.11984401242199283 1103 20-21 0.1687801425980524 1103 22-23 0.3008626460102164 1103 24-25 0.2032147714273549 1103 26-27 0.07463459881738999 1103 28-29 0.14977577248728124 1103 30-31 0.12677471447276645 1103 32-33 0.09171175583522029 1103 34-35 0.22737190702191157 1103 36-37 0.144162704127055 1103 38-39 0.21426908528602695 1103 40-41 0.32064440905475067 1103 42-43 0.1325515456069013 1103 44-45 0.24765378786509729 1103 46-47 0.20686466379822832 1103 48-49 0.17751056581583669 1103 50-51 0.22321935523033432 1103 52-53 0.08458649014193043 1103 54-55 0.297678824323242 1103 56-57 0.21853596995985214 1103 58-59 0.3638859039996376 1103 60-61 0.14617030745520765 1103 62-63 -0.041254439102431206 1103 64-65 -0.09893423292139403 1103 66-67 -0.09297923721284462 1103 68-69 0.02043876102163722 1103 70-71 0.05884343551499427 1103 72-73 -0.15175491760782123 1103 74-75 -0.1158967438678502 1103 76-77 0.012338184254119255 1103 78-79 -0.055402421235807964 1103 80-81 -0.16425569774600746 1103 82-83 -0.07907458049120208 1103 84-85 0.1990574719158431 1103 86-87 0.100852096217519 1103 88-89 0.09438333391834419 1103 90-91 -0.032990171799244905 1103 92-93 0.16820262931113206 1103 94-95 -0.19713120872237155 1103 96-97 0.12250212599251142 1103 98-99 0.1861339422421402 1103 100-101 0.45492168795721 1104 1 0.14912851356217516 1104 2 0.14758647101853484 1104 3 0.0914253057999872 1104 4 0.0349500017949822 1104 5 0.08987434046924392 1104 6 -0.03845677925380642 1104 7 0.05447611609145042 1104 8 0.12071950677577092 1104 9 0.08305774718725445 1104 10-11 0.14307098500929527 1104 12-13 0.1252607747156631 1104 14-15 0.25586971696738203 1104 16-17 0.10788649115965399 1104 18-19 0.19662523289687073 1104 20-21 0.2376139718799095 1104 22-23 0.4075195408720731 1104 24-25 0.3491786039762985 1104 26-27 0.38133564339978676 1104 28-29 0.17882717159192651 1104 30-31 0.1933860468230364 1104 32-33 0.2017986424852225 1104 34-35 0.3989129971668106 1104 36-37 0.449326455440513 1104 38-39 0.3353920775774881 1104 40-41 0.4114104950054269 1104 42-43 0.354391529359809 1104 44-45 0.2057106703650362 1104 46-47 0.0026488069846024587 1104 48-49 0.05879594077941164 1104 50-51 0.024419786131609555 1104 52-53 0.04200938265501719 1104 54-55 0.19603998669708744 1104 56-57 0.18344930405844906 1104 58-59 0.19966748650222144 1104 60-61 0.04472881907982895 1104 62-63 0.26596359870738695 1104 64-65 0.4079963902077637 1104 66-67 0.3553112856081455 1104 68-69 0.18161173079673176 1104 70-71 0.1738013608313338 1104 72-73 0.14041689544459857 1104 74-75 0.028444144954320905 1104 76-77 -0.06071459232180487 1104 78-79 0.1641960812056702 1104 80-81 0.2852930921101553 1104 82-83 0.1571926489372366 1104 84-85 0.2079765984577726 1104 86-87 0.13809061949766388 1104 88-89 0.06657121746903982 1104 90-91 -0.030786217750272016 1104 92-93 0.2997767434469907 1104 94-95 0.23846847693161877 1104 96-97 0.22783629522722038 1104 98-99 0.16679371566577217 1104 100-101 0.35855123648216747 1105 1 0.16869140931474647 1105 2 0.18162056144984007 1105 3 0.15021994753091406 1105 4 0.14560935816360399 1105 5 0.13359485611434962 1105 6 0.15382704418904325 1105 7 0.1687976290540547 1105 8 0.15707870925813694 1105 9 0.06472510059587933 1105 10-11 0.0316585950631989 1105 12-13 0.06157751131788558 1105 14-15 0.2293802577556434 1105 16-17 0.21310180578557691 1105 18-19 0.2948311545746449 1105 20-21 0.3353569734187687 1105 22-23 0.2948924585723631 1105 24-25 0.292577472113436 1105 26-27 0.3105454416658304 1105 28-29 0.2608922747293576 1105 30-31 0.2683345411763298 1105 32-33 0.2407785363625763 1105 34-35 0.26237215844465567 1105 36-37 0.1758512328099897 1105 38-39 0.1532664290267931 1105 40-41 0.16101610891069384 1105 42-43 -7.064785279169428E-4 1105 44-45 0.0631884493574617 1105 46-47 0.3708183173235753 1105 48-49 0.40901709971953437 1105 50-51 0.15364009575412751 1105 52-53 0.10146666029778117 1105 54-55 0.21797340356066996 1105 56-57 0.29452827377733115 1105 58-59 0.14006893622850924 1105 60-61 0.22849802332809332 1105 62-63 0.22941140772024227 1105 64-65 0.16634366860734673 1105 66-67 0.047180120697582595 1105 68-69 0.2745909855836004 1105 70-71 0.11097792639276349 1105 72-73 0.09172198546385957 1105 74-75 0.08014109834276084 1105 76-77 0.09170175830341165 1105 78-79 0.26762993113713307 1105 80-81 0.323225213974613 1105 82-83 0.48337265381450223 1105 84-85 0.49500894820844366 1105 86-87 0.4457269097456482 1105 88-89 0.20557129315080758 1105 90-91 0.2644239673336912 1105 92-93 0.21512164207546647 1105 94-95 0.4860033536123858 1105 96-97 0.5629568394632152 1105 98-99 0.663440172497733 1105 100-101 0.8117668706533259 1106 1 -0.16840203987736402 1106 2 -0.00641942438767984 1106 3 -0.021743309441951908 1106 4 -0.05247920364998748 1106 5 -0.08468930010969444 1106 6 -0.0853252587026816 1106 7 0.004878527246354736 1106 8 -0.14550610843305378 1106 9 -0.0351882038610043 1106 10-11 0.013633698220964163 1106 12-13 -0.16049006157089707 1106 14-15 -0.16533966931420707 1106 16-17 -0.18749406062386953 1106 18-19 -0.00863840437004626 1106 20-21 -0.006540302289593569 1106 22-23 0.007907047910137521 1106 24-25 -0.023594886567863682 1106 26-27 -0.0064603689209548065 1106 28-29 0.11844314218114249 1106 30-31 0.034846815875461346 1106 32-33 -0.04595663191611976 1106 34-35 0.0434175180822578 1106 36-37 -0.1734230229153937 1106 38-39 -0.05863633960530734 1106 40-41 -0.24810661160868364 1106 42-43 -0.17170475123503337 1106 44-45 -0.10191692543531161 1106 46-47 0.052840904490643936 1106 48-49 0.047192257806543125 1106 50-51 -0.04541014133501875 1106 52-53 -0.06031731558629616 1106 54-55 -0.53480077109446 1106 56-57 -0.4407665737940363 1106 58-59 -0.3652208982227094 1106 60-61 -0.3425819814368438 1106 62-63 -0.3159966238607552 1106 64-65 -0.5740217928147899 1106 66-67 -0.5296885487905527 1106 68-69 -0.762046567484326 1106 70-71 -0.7347966585771388 1106 72-73 -0.6098273664704124 1106 74-75 -0.5277609356935145 1106 76-77 -0.340811800283916 1106 78-79 -0.42133284934114457 1106 80-81 -0.8641554555664612 1106 82-83 -0.7286169758102226 1106 84-85 -0.7778164661598517 1106 86-87 -0.5041824239229946 1106 88-89 -0.24689134007740776 1106 90-91 -0.26197970630722267 1106 92-93 -0.34233235468834167 1106 94-95 -0.25016878998455283 1106 96-97 -0.04554164005579153 1106 98-99 -0.07856447994248228 1106 100-101 -0.25674783946259083 1107 1 0.12884571776154985 1107 2 0.08719837555525345 1107 3 0.07400830021973803 1107 4 0.006575841197232535 1107 5 0.02778852439686119 1107 6 -0.020551107574370064 1107 7 0.0773567239290216 1107 8 -0.026682681187281787 1107 9 0.03488945688806666 1107 10-11 0.024284138009072365 1107 12-13 -0.024571344834136255 1107 14-15 0.13293157263747446 1107 16-17 0.10011433566925376 1107 18-19 0.14383904796039104 1107 20-21 0.07325270576181708 1107 22-23 0.12243804397746771 1107 24-25 0.11366676564595934 1107 26-27 0.08865854134738527 1107 28-29 0.11520520999162187 1107 30-31 0.07044080895298066 1107 32-33 0.03341612727352583 1107 34-35 0.06344214373511647 1107 36-37 0.04833966518861388 1107 38-39 0.22608614107254965 1107 40-41 0.21438268832804397 1107 42-43 0.2977054736834006 1107 44-45 0.18948301501650633 1107 46-47 0.1704299476258555 1107 48-49 0.03023498362439625 1107 50-51 -0.04648014766201669 1107 52-53 0.09503674795657702 1107 54-55 0.1284046577144622 1107 56-57 0.26450561100381265 1107 58-59 0.3288518870192618 1107 60-61 0.06399250961231218 1107 62-63 0.193365156133531 1107 64-65 0.33016922037265317 1107 66-67 0.18721724102918813 1107 68-69 0.38787217406417085 1107 70-71 0.3847230481480821 1107 72-73 0.38067904116168094 1107 74-75 0.36667596184640416 1107 76-77 0.13660641401508755 1107 78-79 0.260526356435026 1107 80-81 0.23667790878154804 1107 82-83 0.3881346710690785 1107 84-85 0.5243595235184628 1107 86-87 0.3934241735189161 1107 88-89 0.5369596282007798 1107 90-91 0.3974775988895942 1107 92-93 0.3547209409311449 1107 94-95 0.40468903873999906 1107 96-97 0.6454668951830804 1107 98-99 0.6588513152117876 1107 100-101 0.6351070862882793 1108 1 0.04126738746035841 1108 2 0.04437627951908496 1108 3 0.08842934511379497 1108 4 0.08427214933623617 1108 5 0.08842214991829422 1108 6 0.06850653985198818 1108 7 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-0.5492583774604967 2316 64-65 -0.6152542387372648 2316 66-67 -0.6088930800989019 2316 68-69 -0.5360672737377001 2316 70-71 -0.5652519827458988 2316 72-73 -0.31372660947397435 2316 74-75 -0.4962880826409233 2316 76-77 -0.4592051242511239 2316 78-79 -0.28996625440241175 2316 80-81 -0.38289660072363674 2316 82-83 -0.3847734256925541 2316 84-85 -0.4287281815244519 2316 86-87 -0.37644742602740777 2316 88-89 -0.3754792186497262 2316 90-91 -0.24793570329583403 2316 92-93 -0.4152606919637485 2316 94-95 -0.17511899538726894 2316 96-97 -0.11952514855940777 2316 98-99 -0.1573570202375727 2316 100-101 -0.17498170215991493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 13.0 5 22.0 6 56.0 7 112.0 8 286.0 9 524.0 10 951.0 11 1500.0 12 2385.0 13 3710.0 14 4781.0 15 6625.0 16 9312.0 17 14068.0 18 13728.0 19 8387.0 20 6169.0 21 6140.0 22 5894.0 23 6269.0 24 7065.0 25 8145.0 26 9592.0 27 11358.0 28 13992.0 29 17253.0 30 20716.0 31 25553.0 32 32306.0 33 42289.0 34 60712.0 35 97184.0 36 149825.0 37 245632.0 38 223938.0 39 26429.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.74714063830298 18.94584526850205 14.9264867480382 21.380527345156768 2 16.06414241885225 20.685034236011447 39.8212745364239 23.429548808712404 3 19.37809249932124 25.788881685754973 30.02220042812473 24.810825386799056 4 12.892937150371123 17.491647690440967 36.233325391944476 33.382089767243436 5 13.15555703302325 38.07840551686437 33.33998193795743 15.426055512154942 6 30.80372395732335 36.29445561779388 17.396073998969467 15.505746425913305 7 27.923031320468283 34.42850626169277 19.874895422989766 17.773566994849176 8 27.148871255640948 34.07342494290946 19.16764626305141 19.610057538398177 9 27.332052856601997 16.723426260238426 20.052912007239616 35.89160887591996 10-11 25.224805943852534 27.20170388961839 25.927434174197135 21.64605599233194 12-13 26.62923132896372 24.842280323639297 27.160334794179512 21.36815355321746 14-15 23.166231737114174 25.697503264280186 26.664736724491057 24.471528274114583 16-17 21.38414841915428 29.181029666655277 27.263367170452835 22.17145474373761 18-19 21.25775901393806 26.535042763455575 31.208226909488484 20.99897131311788 20-21 22.217882160870374 25.410828360731124 31.66587252778101 20.70541695061749 22-23 21.882681677738443 25.481469784862238 31.64024769745894 20.995600839940384 24-25 21.616758421251664 25.80063821112497 31.664081716142416 20.918521651480948 26-27 21.029290538590313 26.160734283900016 31.576562950990013 21.233412226519658 28-29 20.924959415809276 26.400870228471913 31.7266795575723 20.947490798146518 30-31 21.13531387877747 25.981454086767986 31.622933622917 21.260298411537544 32-33 20.859768138612644 26.180141587902835 31.474474608966496 21.485615664518022 34-35 21.585161759870548 26.318074176422996 31.133094920551653 20.963669143154807 36-37 21.683247906894493 25.8694589207411 31.178810980325522 21.26848219203888 38-39 21.139351980342276 26.26108367787153 31.205993740818922 21.393570600967276 40-41 21.436131501464782 26.073879987735005 31.260644134043126 21.22934437675709 42-43 21.254278657481606 26.16887635401357 30.932621505099295 21.64422348340553 44-45 21.26213112581742 26.411052108376854 30.87043987638822 21.45637688941751 46-47 21.617014517966933 26.885525803049866 30.013278740902035 21.484180938081177 48-49 21.642962544347117 26.868211728507923 29.81026544553962 21.678560281605343 50-51 21.695874356147282 27.168968746017764 29.55928062097967 21.575876276855283 52-53 21.960433415148106 27.149484640799905 29.334890214916143 21.55519172913585 54-55 21.410953945485137 27.18951478114086 29.544321260575433 21.855210012798565 56-57 21.6077803448779 27.47341943276321 29.28590292667882 21.63289729568007 58-59 21.638760995591603 26.96517054594278 29.33664470780306 22.059423750662553 60-61 21.372539785434753 27.22026457752735 29.703610746361274 21.703584890676623 62-63 21.41695615799301 27.291275368582017 29.58305861668393 21.708709856741038 64-65 21.168326047570766 27.235962671778708 29.495149288876334 22.1005619917742 66-67 21.11984663885334 27.37530634369223 29.336136828283166 22.16871018917127 68-69 21.25725693928226 27.283552291978513 29.47450848578254 21.984682282956687 70-71 21.23222652534688 27.488332622302003 29.129660718012364 22.14978013433875 72-73 21.337726952889096 27.426001953951022 28.99137897600408 22.2448921171558 74-75 21.21177283195467 27.88378608483989 28.684065695601024 22.220375387604417 76-77 21.32355249719743 27.40319354642111 29.00010526957322 22.273148686808245 78-79 21.330662810475985 27.583629288580834 28.972541262902446 22.11316663804073 80-81 21.361735802920585 27.865086884334595 28.711583531406347 22.061593781338477 82-83 21.43671728840354 27.834106233620886 28.6651356407685 22.06404083720707 84-85 21.336298843654134 27.71046842996637 28.602199291897453 22.351033434482044 86-87 21.400911692855637 27.754551192343367 28.529901041664264 22.314636073136736 88-89 21.359390950598538 27.78956417914858 28.369332004227406 22.48171286602548 90-91 21.27326554206543 27.840713913708342 28.55437829891443 22.3316422453118 92-93 21.364242043121862 27.465600301404248 28.64397639369452 22.526181261779374 94-95 21.144271026673835 27.395067474102763 28.70858242515241 22.752079074071 96-97 21.27918229550462 27.23780950639651 28.66841377221511 22.814594425883755 98-99 21.10922733980095 27.352682619624098 28.384093582803754 23.153996457771203 100-101 21.07021295849978 27.17460159160207 28.742795036447284 23.012390413450866 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2855.0 1 1807.0 2 1410.5 3 1801.5 4 1126.0 5 868.0 6 1680.0 7 3442.0 8 5193.5 9 5771.0 10 4487.0 11 2923.0 12 2656.5 13 2712.5 14 2722.5 15 2789.0 16 2825.5 17 2879.5 18 3014.0 19 3148.5 20 3350.0 21 3738.0 22 4225.0 23 4767.0 24 5409.0 25 6367.0 26 7628.5 27 9013.0 28 10249.5 29 11525.0 30 13175.5 31 15165.0 32 17735.5 33 19338.0 34 20026.0 35 20898.0 36 22504.5 37 24338.0 38 25266.0 39 26077.0 40 27121.0 41 29335.0 42 30986.5 43 32056.0 44 33837.0 45 35813.5 46 36630.5 47 37781.5 48 40338.0 49 40403.0 50 39046.5 51 37512.0 52 35899.5 53 36058.0 54 36540.0 55 34348.0 56 30956.5 57 28103.5 58 25681.0 59 22646.0 60 18451.5 61 15188.5 62 13018.5 63 10008.0 64 7410.5 65 5934.0 66 4296.5 67 3307.5 68 2509.5 69 1637.5 70 1179.5 71 888.0 72 709.5 73 586.5 74 439.5 75 322.0 76 206.5 77 118.0 78 69.0 79 34.0 80 18.0 81 12.0 82 6.5 83 5.5 84 3.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014405310018893117 3 0.006648604624104516 4 0.0 5 0.0 6 0.0 7 0.0 8 4.6170865445170254E-4 9 3.6936692356136203E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 9.234173089034051E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005863699911536622 26-27 0.022762236664468933 28-29 0.0 30-31 0.0 32-33 2.3085432722585127E-4 34-35 0.02013049733409423 36-37 0.0030934479848264067 38-39 0.018883883967074633 40-41 0.01643682809848061 42-43 0.008172243183795135 44-45 0.0025393975994843637 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.6170865445170254E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0036936692356136203 86-87 3.2319605811619175E-4 88-89 4.6170865445170254E-5 90-91 1.8468346178068102E-4 92-93 2.7702519267102147E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1082934.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.21319153761942 #Duplication Level Percentage of deduplicated Percentage of total 1 81.83178385342919 49.273528750316046 2 10.9841344197725 13.227795793854396 3 3.0144299691956893 5.4452534733576075 4 1.324025809293356 3.188952786229296 5 0.696333701330773 2.096423726616466 6 0.44978350455149635 1.6249740186012582 7 0.294103555929766 1.2396239621565779 8 0.20199082539606433 0.9730009806732051 9 0.15832144647801646 0.8579735623164392 >10 0.9303993376876981 10.357669498087422 >50 0.07667867828021951 3.215422189453028 >100 0.033722895379214475 3.9493523974827838 >500 0.0030657166256863747 1.2136302308738645 >1k 7.664291564215937E-4 0.9419040616067982 >5k 3.065716625686375E-4 1.406533659463596 >10k+ 1.5328583128431875E-4 0.9879609089112364 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10704 0.9884258874502048 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9404 0.8683816372927622 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5835 0.5388139997451368 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2683 0.24775286397878357 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2404 0.22198952106037856 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1977 0.18255960197020318 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1907 0.17609568080787932 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1234 0.11394969591868019 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.23417308903405E-5 0.0 10-11 0.0 0.0 0.0 9.23417308903405E-5 0.0 12-13 0.0 0.0 0.0 9.23417308903405E-5 2.7702519267102147E-4 14-15 0.0 0.0 0.0 9.23417308903405E-5 3.69366923561362E-4 16-17 0.0 0.0 0.0 2.770251926710215E-4 3.69366923561362E-4 18-19 4.617086544517025E-5 0.0 0.0 2.770251926710215E-4 3.69366923561362E-4 20-21 9.23417308903405E-5 0.0 0.0 3.2319605811619175E-4 3.69366923561362E-4 22-23 9.23417308903405E-5 0.0 0.0 5.078795198968728E-4 3.69366923561362E-4 24-25 9.23417308903405E-5 0.0 0.0 0.0011081007706840859 3.69366923561362E-4 26-27 9.23417308903405E-5 0.0 0.0 0.001708322021471299 4.617086544517025E-4 28-29 9.23417308903405E-5 0.0 0.0 0.006325408565988325 4.617086544517025E-4 30-31 1.84683461780681E-4 0.0 0.0 0.018191320985397077 5.078795198968728E-4 32-33 1.84683461780681E-4 0.0 0.0 0.04178463322787908 5.54050385342043E-4 34-35 1.84683461780681E-4 0.0 0.0 0.07664363663898262 6.463921162323835E-4 36-37 1.84683461780681E-4 0.0 0.0 0.13080206180616732 6.463921162323835E-4 38-39 1.84683461780681E-4 0.0 0.0 0.21737243451586152 6.925629816775538E-4 40-41 1.84683461780681E-4 0.0 0.0 0.3052817623234657 7.38733847122724E-4 42-43 1.84683461780681E-4 0.0 0.0 0.3908363759933662 7.38733847122724E-4 44-45 1.84683461780681E-4 0.0 0.0 0.4910733248748308 7.38733847122724E-4 46-47 3.2319605811619175E-4 0.0 0.0 0.6095477656071376 7.38733847122724E-4 48-49 3.69366923561362E-4 0.0 0.0 0.7340705897127617 7.38733847122724E-4 50-51 3.69366923561362E-4 0.0 0.0 0.8667656570021811 7.38733847122724E-4 52-53 3.69366923561362E-4 0.0 0.0 0.9918887023585925 7.38733847122724E-4 54-55 3.69366923561362E-4 0.0 0.0 1.1318787663883487 7.38733847122724E-4 56-57 3.69366923561362E-4 0.0 0.0 1.290568030923399 7.849047125678942E-4 58-59 3.69366923561362E-4 0.0 0.0 1.462231308648542 8.310755780130645E-4 60-61 3.69366923561362E-4 0.0 0.0 1.646129865716655 8.310755780130645E-4 62-63 3.69366923561362E-4 0.0 0.0 1.8221332047936438 8.310755780130645E-4 64-65 3.69366923561362E-4 0.0 0.0 1.9967514179072778 8.310755780130645E-4 66-67 3.69366923561362E-4 0.0 0.0 2.195655506245071 9.23417308903405E-4 68-69 3.69366923561362E-4 0.0 0.0 2.414782433647849 9.23417308903405E-4 70-71 4.617086544517025E-4 0.0 0.0 2.6426356546197645 9.23417308903405E-4 72-73 5.078795198968728E-4 0.0 0.0 2.8799077321424944 0.0010619299052389156 74-75 5.54050385342043E-4 0.0 0.0 3.117641518319676 0.0012004425015744264 76-77 5.54050385342043E-4 0.0 0.0 3.3664101413382532 0.0012004425015744264 78-79 5.54050385342043E-4 0.0 0.0 3.623628032733297 0.0012004425015744264 80-81 5.54050385342043E-4 0.0 0.0 3.9171823952336893 0.0012004425015744264 82-83 5.54050385342043E-4 0.0 0.0 4.217847071012638 0.0012004425015744264 84-85 5.54050385342043E-4 0.0 0.0 4.520589435736619 0.0012004425015744264 86-87 5.54050385342043E-4 0.0 0.0 4.824670755558511 0.0012004425015744264 88-89 5.54050385342043E-4 0.0 0.0 5.141449063377824 0.0012004425015744264 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4770 0.0 44.72165 1 GTATCAA 7805 0.0 37.010124 1 TATCAAC 9875 0.0 29.059605 2 ATCAACG 9990 0.0 28.813549 3 TCAACGC 10120 0.0 28.442102 4 CAACGCA 10260 0.0 28.054003 5 AACGCAG 10490 0.0 27.4389 6 TACCTGG 940 0.0 24.260695 2 GTACATG 6920 0.0 24.029892 1 TACATGG 6865 0.0 24.01479 2 CGCAGAG 11970 0.0 24.006607 8 ACGCAGA 12015 0.0 23.877161 7 GCAGAGT 12685 0.0 22.50368 9 GAGTACT 7580 0.0 22.370132 12-13 ACATGGG 7015 0.0 22.141634 3 GTACCTG 1115 0.0 21.731276 1 AGTACTT 7790 0.0 21.401253 12-13 GTGGTAT 2550 0.0 21.240002 1 TGGTATC 2550 0.0 21.240002 2 CTACACG 135 3.797171E-4 21.10994 4 >>END_MODULE