FastQCFastQC Report
Thu 26 May 2016
SRR938168_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938168_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106143
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107380.9707605616995271No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89460.8087561915593191No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56100.5071676989322358No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26920.24336817210794626No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22950.20747769501773278No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19160.17321449396687408No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17600.15911143495913277No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.11833912975085499No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49550.047.0159841
GTATCAA79250.037.451491
TATCAAC101350.029.3350162
CTTAGCG650.005802273829.2736363
ATCAACG101150.029.251933
TCAACGC102650.028.7781414
CAACGCA104500.028.2231485
AACGCAG107500.027.4293086
GTACATG70950.026.5231441
TACATGG71600.025.7779712
ACGCAGA119800.024.5225967
CGCAGAG119900.024.461398
ACATGGG73250.024.1582453
GAGTACT74250.023.94798712-13
GCAGAGT123800.023.2289839
GTACTTT78050.022.50435314-15
TAAGGTA3650.022.1557334
TCGTATG1100.002828248321.6549721
AGTACTT75850.021.40567812-13
CATGGGG32150.021.158514
Produced by FastQC (version 0.11.5)