##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938168_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1106143 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01051220321423 33.0 31.0 34.0 30.0 34.0 2 32.09834533148065 33.0 31.0 34.0 30.0 34.0 3 32.10095801356606 34.0 31.0 34.0 30.0 34.0 4 35.633369284079905 37.0 35.0 37.0 35.0 37.0 5 35.653516769531606 37.0 35.0 37.0 35.0 37.0 6 35.70694837828382 37.0 36.0 37.0 35.0 37.0 7 35.651326275174185 37.0 35.0 37.0 33.0 37.0 8 35.64558922309322 37.0 35.0 37.0 33.0 37.0 9 37.27787546456471 39.0 37.0 39.0 34.0 39.0 10-11 37.19088173952193 39.0 37.0 39.0 34.0 39.0 12-13 37.03579148446448 39.0 37.0 39.0 33.0 39.0 14-15 38.18041293033541 40.0 38.0 41.0 33.0 41.0 16-17 38.02114735617366 40.0 38.0 41.0 33.0 41.0 18-19 38.23823999247837 40.0 38.0 41.0 33.5 41.0 20-21 38.26132064299101 40.0 38.0 41.0 34.0 41.0 22-23 38.14424265217065 40.0 38.0 41.0 33.5 41.0 24-25 38.012210898590865 40.0 38.0 41.0 33.0 41.0 26-27 37.81733871660355 40.0 38.0 41.0 33.0 41.0 28-29 37.638055839073246 40.0 37.5 41.0 32.5 41.0 30-31 37.3684641135911 40.0 37.0 41.0 32.0 41.0 32-33 37.02114780819478 39.5 36.0 41.0 31.0 41.0 34-35 36.76127634492104 39.0 35.0 41.0 31.0 41.0 36-37 36.476014403201034 39.0 35.0 41.0 30.0 41.0 38-39 36.18062899643175 39.0 35.0 40.5 30.0 41.0 40-41 36.019308082228065 39.0 35.0 41.0 30.0 41.0 42-43 35.75623811749476 39.0 35.0 41.0 28.5 41.0 44-45 35.343323150804196 38.5 35.0 40.0 26.5 41.0 46-47 34.82100958013566 38.0 34.5 40.0 24.0 41.0 48-49 34.30389063620165 38.0 34.0 40.0 22.0 41.0 50-51 33.51945046888151 37.5 33.0 39.5 20.0 40.5 52-53 33.57115580896864 38.0 33.0 40.0 19.5 40.5 54-55 33.65064824349112 38.0 33.5 40.0 18.0 41.0 56-57 33.340889921104235 38.0 33.0 40.0 14.0 41.0 58-59 32.94846778400261 37.0 33.0 40.0 10.0 41.0 60-61 32.46181822784215 36.5 32.0 40.0 7.5 41.0 62-63 31.93157259052401 36.0 31.0 39.0 2.0 41.0 64-65 31.41637021614746 35.5 31.0 39.0 2.0 40.5 66-67 30.882079441808155 35.0 30.0 38.5 2.0 40.0 68-69 30.246179743487055 35.0 29.0 38.0 2.0 40.0 70-71 29.68276524825452 35.0 29.0 37.0 2.0 39.5 72-73 29.01969184816068 34.0 28.0 36.5 2.0 39.0 74-75 28.373503697080757 34.0 26.0 36.0 2.0 39.0 76-77 27.65761253291844 33.5 26.0 35.5 2.0 38.0 78-79 26.93489675385551 33.0 24.5 35.0 2.0 37.0 80-81 26.25049564116032 32.5 22.5 35.0 2.0 36.5 82-83 25.547041838171012 32.0 19.5 35.0 2.0 36.0 84-85 24.8564493921672 31.5 17.0 35.0 2.0 35.5 86-87 24.138708105552354 31.0 8.5 34.0 2.0 35.0 88-89 23.294131952197862 30.5 2.0 34.0 2.0 35.0 90-91 22.446656987387705 30.0 2.0 34.0 2.0 35.0 92-93 21.5448002654268 29.0 2.0 34.0 2.0 35.0 94-95 20.508780962316806 28.5 2.0 34.0 2.0 35.0 96-97 19.286561050424766 26.5 2.0 33.5 2.0 35.0 98-99 17.777036965383317 23.5 2.0 33.0 2.0 35.0 100-101 13.865256571709082 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24956812580501264 1101 2 0.2772746566755231 1101 3 0.21878683835533508 1101 4 0.0533112788709218 1101 5 0.10891527228445597 1101 6 0.016205979123242287 1101 7 0.10686575229279072 1101 8 0.1449437819398156 1101 9 0.23767565060937557 1101 10-11 0.24732308885429433 1101 12-13 0.28219143421684834 1101 14-15 0.34628036847142596 1101 16-17 0.4022532964030674 1101 18-19 0.42368512920145207 1101 20-21 0.3802976623194141 1101 22-23 0.322506223671688 1101 24-25 0.43716758885206275 1101 26-27 0.45312906659396646 1101 28-29 0.5204661946095257 1101 30-31 0.5043310972038739 1101 32-33 0.5334583740154031 1101 34-35 0.4512431064848954 1101 36-37 0.48271814814806646 1101 38-39 0.5440699374584312 1101 40-41 0.6367526084101698 1101 42-43 0.8593960474761033 1101 44-45 0.8350558549558826 1101 46-47 0.5201616016130188 1101 48-49 0.5676971076919415 1101 50-51 0.5329342160988872 1101 52-53 0.6028362537269913 1101 54-55 0.612084474014452 1101 56-57 0.545081450905748 1101 58-59 0.6853308583002189 1101 60-61 0.583382500172597 1101 62-63 0.47132984339778616 1101 64-65 0.30741671193316833 1101 66-67 0.32614117952480015 1101 68-69 0.30498124312551766 1101 70-71 0.3441936924776847 1101 72-73 0.3269489356365973 1101 74-75 0.4567886845429676 1101 76-77 0.3479635722316523 1101 78-79 0.1146886412001571 1101 80-81 -0.13083651052526335 1101 82-83 0.025548481901171982 1101 84-85 -0.06948684430139096 1101 86-87 -0.14658675281383893 1101 88-89 -0.2765250061469722 1101 90-91 -0.6529518742533682 1101 92-93 -0.5887975284715985 1101 94-95 -0.5862363384129416 1101 96-97 -0.10092888248420806 1101 98-99 0.013144302181395062 1101 100-101 -0.24377939323767883 1102 1 0.2556119505062142 1102 2 0.30376071245241576 1102 3 0.3880458024987661 1102 4 0.3430682509824763 1102 5 0.3169590969856557 1102 6 0.44810239346587366 1102 7 0.3489255132489646 1102 8 0.39812704887208383 1102 9 0.45780712471296425 1102 10-11 0.35410994144393015 1102 12-13 0.46946235055150254 1102 14-15 0.4953978983519036 1102 16-17 0.4489903481958919 1102 18-19 0.3773883164524534 1102 20-21 0.5118813276580667 1102 22-23 0.621880725663722 1102 24-25 0.504476353792306 1102 26-27 0.4326948036457594 1102 28-29 0.46905782807565544 1102 30-31 0.5158151609488968 1102 32-33 0.45364960907516405 1102 34-35 0.4795917120625788 1102 36-37 0.44260460233132903 1102 38-39 0.23347631196041618 1102 40-41 0.6478920506412464 1102 42-43 0.732888079348605 1102 44-45 0.5347909147168366 1102 46-47 0.57953809563692 1102 48-49 0.5532608527118654 1102 50-51 0.6947748535490845 1102 52-53 0.4986350585078725 1102 54-55 0.5627179401499092 1102 56-57 0.45519499672248287 1102 58-59 0.4464424120850765 1102 60-61 0.45569126511283997 1102 62-63 0.49297526172447803 1102 64-65 0.4389087438056798 1102 66-67 0.34954157793117346 1102 68-69 0.3526824383446403 1102 70-71 -0.021140968876895982 1102 72-73 0.012391166712294677 1102 74-75 -0.180320877210022 1102 76-77 -0.37543682617472385 1102 78-79 -0.561526498242074 1102 80-81 -0.43628870176032564 1102 82-83 -0.6295112790550057 1102 84-85 -0.6027159279667309 1102 86-87 -0.6034652388696138 1102 88-89 -0.7142979145135051 1102 90-91 -0.6236490854087471 1102 92-93 -0.6008692416190122 1102 94-95 -0.5548478921978024 1102 96-97 -1.0700364522053256 1102 98-99 -1.2184572914441034 1102 100-101 -1.169157879293456 1103 1 0.43284271973698196 1103 2 0.3115299432216432 1103 3 0.2934688794218445 1103 4 0.2381067125209384 1103 5 0.27872832775488376 1103 6 0.19594854731202815 1103 7 0.2544255132489681 1103 8 0.11162704887208719 1103 9 0.26249943240527074 1103 10-11 0.3116676337516182 1103 12-13 0.1197508120899684 1103 14-15 0.24334020604421625 1103 16-17 0.37424034819589735 1103 18-19 0.25173447029860796 1103 20-21 0.15938132765806046 1103 22-23 0.23513072566372273 1103 24-25 0.2926686614846119 1103 26-27 0.3035217267226855 1103 28-29 0.3326539819218084 1103 30-31 0.3991805455642776 1103 32-33 0.500553455229003 1103 34-35 0.3785147889856617 1103 36-37 0.2870661407928665 1103 38-39 0.29343785042195947 1103 40-41 0.6220843583335594 1103 42-43 0.5238880793486089 1103 44-45 0.5921562993322169 1103 46-47 0.7217111725599992 1103 48-49 0.8262800834810946 1103 50-51 0.7549863920106219 1103 52-53 0.7545773662001807 1103 54-55 0.910448709380681 1103 56-57 0.8561180736455682 1103 58-59 0.9132116428543071 1103 60-61 0.7994604958820659 1103 62-63 0.6859368001860169 1103 64-65 0.8421010514979876 1103 66-67 0.6645031163927122 1103 68-69 0.7364132075754135 1103 70-71 0.5115128772769459 1103 72-73 0.596679628250758 1103 74-75 0.36879450740536157 1103 76-77 0.3980631738252818 1103 78-79 -0.03200726747284577 1103 80-81 -0.14230793252955465 1103 82-83 -0.027780509824236077 1103 84-85 -0.178081312582119 1103 86-87 -0.3697537004080793 1103 88-89 -0.13666329912888742 1103 90-91 -0.15509139310105624 1103 92-93 -0.1449077031574717 1103 94-95 -0.4088286614285721 1103 96-97 -0.709132606051476 1103 98-99 -0.812899599136415 1103 100-101 -0.9861771100626875 1104 1 0.2441064560007149 1104 2 0.24825155494325202 1104 3 0.280419428872392 1104 4 0.24135762827185658 1104 5 0.17946092848748663 1104 6 0.27745037881386025 1104 7 0.3538302751537259 1104 8 0.33630653605157335 1104 9 0.35773752764336564 1104 10-11 0.3230458388798283 1104 12-13 0.3550301161192735 1104 14-15 0.46845467490867776 1104 16-17 0.38145188665743746 1104 18-19 0.48827293183706644 1104 20-21 0.3383190565957932 1104 22-23 0.4683495901325827 1104 24-25 0.6434012622172105 1104 26-27 0.5003394922904434 1104 28-29 0.4293343848522113 1104 30-31 0.4483334759672104 1104 32-33 0.5694408178663721 1104 34-35 0.6452272431981143 1104 36-37 0.6168738331005557 1104 38-39 0.36738473686884277 1104 40-41 0.5556466293958238 1104 42-43 0.2986591416196731 1104 44-45 0.31978908321500654 1104 46-47 0.26257655717538597 1104 48-49 0.42721872816974127 1104 50-51 0.29255507332930364 1104 52-53 0.33674311711592964 1104 54-55 0.2339377203696884 1104 56-57 0.4823543373818282 1104 58-59 0.44819332783599464 1104 60-61 0.4095795435011169 1104 62-63 0.41849174524096 1104 64-65 0.18578237017930732 1104 66-67 0.26679249368208957 1104 68-69 0.3998289584911596 1104 70-71 0.3399469432110145 1104 72-73 0.4787538040749304 1104 74-75 0.2781351667460221 1104 76-77 0.12068222144432639 1104 78-79 0.038436871721298616 1104 80-81 -0.10890591787754289 1104 82-83 -0.4237969933407193 1104 84-85 -0.2260437667945716 1104 86-87 -0.21267952458390127 1104 88-89 -0.23714406835965818 1104 90-91 -0.34174432350398476 1104 92-93 -0.3588729046226753 1104 94-95 -0.7891171229670348 1104 96-97 -1.3437250968939658 1104 98-99 -1.3231789031657186 1104 100-101 -1.6271798573154346 1105 1 0.10226914573993895 1105 2 0.19265347138917122 1105 3 0.2193528602439514 1105 4 0.05151096408971512 1105 5 0.1939719292496349 1105 6 0.05195573902041417 1105 7 0.20077060949094516 1105 8 0.22905372164935045 1105 9 0.32442215679362363 1105 10-11 0.22315806243384628 1105 12-13 0.2971846236954079 1105 14-15 0.17849276544631465 1105 16-17 0.25763287798508117 1105 18-19 0.20160050710322963 1105 20-21 0.25546290419335094 1105 22-23 0.17697880082412354 1105 24-25 0.14675453894354007 1105 26-27 0.22977454700048128 1105 28-29 0.1406604861874854 1105 30-31 0.08061121961067386 1105 32-33 -0.07484534967827727 1105 34-35 -0.0038047132353113966 1105 36-37 0.0624336582433358 1105 38-39 0.015459813335304773 1105 40-41 0.014472252291106713 1105 42-43 0.39724967421570767 1105 44-45 0.38227166632087517 1105 46-47 0.33781628078815373 1105 48-49 0.23679751632322876 1105 50-51 0.2163358068029808 1105 52-53 0.17902873403491526 1105 54-55 0.24072527287218293 1105 56-57 0.23610379598921583 1105 58-59 0.14426642675051937 1105 60-61 0.16430308912750746 1105 62-63 0.012312566949045589 1105 64-65 -0.0029193955160415896 1105 66-67 -0.0231564055702016 1105 68-69 -0.04779235542254412 1105 70-71 -0.43820421360650386 1105 72-73 -0.4272843603271177 1105 74-75 -0.5287677149735437 1105 76-77 -0.8212997959272421 1105 78-79 -0.7122551875179681 1105 80-81 -0.6949197741709554 1105 82-83 -0.9163169619147666 1105 84-85 -1.0375367345661815 1105 86-87 -0.8543254588512816 1105 88-89 -0.9579541931569509 1105 90-91 -0.8507847407707985 1105 92-93 -0.9526290949645677 1105 94-95 -0.8901535750575604 1105 96-97 -1.1265112459725088 1105 98-99 -1.428116319858404 1105 100-101 -1.6553448637114219 1106 1 0.16900819659588961 1106 2 0.21962932559110016 1106 3 0.2564962717375536 1106 4 0.2524936941524487 1106 5 0.34483083838293993 1106 6 0.2149386812656644 1106 7 0.29187337560559 1106 8 0.2928183940232856 1106 9 0.4409906492176532 1106 10-11 0.3960218288266262 1106 12-13 0.4081536957027012 1106 14-15 0.5108499317199957 1106 16-17 -0.109173364004306 1106 18-19 0.3349581808945814 1106 20-21 0.5049756967925774 1106 22-23 0.43573682576799655 1106 24-25 0.454215144198983 1106 26-27 0.44269063263428876 1106 28-29 0.2359572024239327 1106 30-31 0.13227240808132024 1106 32-33 0.24581644953396875 1106 34-35 0.3235546943357832 1106 36-37 0.44436841880682465 1106 38-39 -0.04331618021893746 1106 40-41 -0.2738900140094316 1106 42-43 -0.3058434117879969 1106 44-45 0.03293846424759295 1106 46-47 0.2495521728006267 1106 48-49 0.33213102997984834 1106 50-51 0.16727433217265286 1106 52-53 0.49750419302299065 1106 54-55 0.2035875960414657 1106 56-57 0.24805578921466775 1106 58-59 0.6653934027003032 1106 60-61 0.8497783349772803 1106 62-63 0.7940732804940289 1106 64-65 0.4045599429714777 1106 66-67 0.33461822026693966 1106 68-69 0.3005635645177378 1106 70-71 -0.13353304395510435 1106 72-73 0.08995738151938681 1106 74-75 0.30417390902563923 1106 76-77 -0.17172306183686814 1106 78-79 -0.16716257749129326 1106 80-81 -0.13066669967481914 1106 82-83 -0.23153943338242655 1106 84-85 -0.050928128175282694 1106 86-87 -0.503868262852933 1106 88-89 -0.9182822732204912 1106 90-91 -0.7554999717486837 1106 92-93 -0.8779599611184885 1106 94-95 -0.9775345449610953 1106 96-97 -1.2937966190457821 1106 98-99 -1.5481361235608944 1106 100-101 -2.010211059689702 1107 1 -0.16407261325217704 1107 2 -0.07074935466167886 1107 3 -0.14041728474955661 1107 4 0.015074962391871338 1107 5 -0.04214157415528774 1107 6 -0.04430700250728137 1107 7 0.06088524479259405 1107 8 -0.0510407363628147 1107 9 -0.19368280817294448 1107 10-11 -0.007695427683586331 1107 12-13 -0.07245040112633916 1107 14-15 0.061025415130423255 1107 16-17 -0.05549958469001126 1107 18-19 -0.09565195200392651 1107 20-21 0.04939810618154894 1107 22-23 -0.033703166953728214 1107 24-25 0.02566427325539422 1107 26-27 -0.1488756661528967 1107 28-29 -0.14464015850152379 1107 30-31 -0.20393316119875493 1107 32-33 -0.08565911575705343 1107 34-35 -0.03775392552130796 1107 36-37 -0.34082492787001684 1107 38-39 -0.20014113770400854 1107 40-41 -0.14265492922452694 1107 42-43 -0.299266283067503 1107 44-45 -0.34912854837040896 1107 46-47 -0.3684249916114055 1107 48-49 -0.18188008688544954 1107 50-51 -0.20323185786030962 1107 52-53 -0.28310655223037884 1107 54-55 -0.5102082343467416 1107 56-57 -0.5038586945526831 1107 58-59 -0.3383629570424418 1107 60-61 -0.5928020234777662 1107 62-63 -0.5008468859265278 1107 64-65 -0.6440308535097579 1107 66-67 -0.5709382878406366 1107 68-69 -0.7113410515882457 1107 70-71 -0.6031812373332706 1107 72-73 -0.4094477594621999 1107 74-75 -0.6370457094247861 1107 76-77 -0.590946893288816 1107 78-79 -0.5857211291145568 1107 80-81 -0.6981578292771005 1107 82-83 -0.9376991985180929 1107 84-85 -1.1959407601814966 1107 86-87 -1.0259585274602223 1107 88-89 -0.9792710688759207 1107 90-91 -0.9891356625899554 1107 92-93 -1.0226309865854546 1107 94-95 -1.0638009123320273 1107 96-97 -1.2376270562321672 1107 98-99 -1.4006351437930995 1107 100-101 -0.76073506050151 1108 1 -0.15532110388709341 1108 2 -0.015578199681478111 1108 3 0.11325082341926418 1108 4 0.18666239324188894 1108 5 0.053398427529586456 1108 6 0.10967561522319613 1108 7 0.1461096136673774 1108 8 -0.051709771211598365 1108 9 0.039535158185771024 1108 10-11 0.11692793307572913 1108 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48-49 -0.581506863823563 2316 50-51 -0.8782093984194219 2316 52-53 -0.8933216344055097 2316 54-55 -0.6905733984327682 2316 56-57 -0.8468876804428689 2316 58-59 -0.5057465642928776 2316 60-61 -0.5201866876430685 2316 62-63 -0.48569402961410546 2316 64-65 -0.31539440580062106 2316 66-67 -0.27376157167512716 2316 68-69 -0.274451419923075 2316 70-71 -0.24753466966430082 2316 72-73 -0.3131363923428232 2316 74-75 -0.38609646776120243 2316 76-77 -0.43717304664716394 2316 78-79 -0.08172334863577291 2316 80-81 -0.15833988286268763 2316 82-83 -0.09514120031484552 2316 84-85 -0.27540490434468623 2316 86-87 -0.2171227191845766 2316 88-89 0.06748948706129809 2316 90-91 -0.09557821926701848 2316 92-93 -0.03031806051664887 2316 94-95 0.10858124166046679 2316 96-97 0.1543100044875878 2316 98-99 -0.16559114971182254 2316 100-101 0.19139330180890646 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3441.0 3 1039.0 4 935.0 5 1071.0 6 1232.0 7 1509.0 8 2046.0 9 2530.0 10 3163.0 11 4111.0 12 5598.0 13 7300.0 14 8482.0 15 10674.0 16 13455.0 17 18230.0 18 17704.0 19 12412.0 20 12230.0 21 12619.0 22 13513.0 23 14737.0 24 16555.0 25 18542.0 26 20926.0 27 23810.0 28 27212.0 29 31649.0 30 37576.0 31 44659.0 32 54275.0 33 67430.0 34 88672.0 35 121082.0 36 144560.0 37 155361.0 38 80510.0 39 5292.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.553229784198194 18.37846255246566 14.553041469147113 21.51526619418903 2 15.82808645967407 20.388773665201672 40.34646099464624 23.436678880478013 3 19.347046680911166 25.758066852569446 29.84264657950965 25.05223988700974 4 12.676488532205592 17.180238715505645 36.457611070780075 33.68566168150869 5 13.1831253821245 38.000588754783635 33.24363125835013 15.57265460474174 6 30.743380338845487 36.174165567616924 17.549620284351946 15.53283380918564 7 28.041842224172107 34.30386864902897 20.007624470384545 17.64666465641438 8 27.205354186531665 33.74176073705104 19.30561310248621 19.747271973931092 9 27.584062706479727 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31.397231234756696 21.54253959015685 34-35 21.643130803657876 26.2999017303067 31.019199690169952 21.037767775865472 36-37 21.65841388187019 25.971819133943942 30.96325719960278 21.40650978458309 38-39 21.275527648281514 26.294043051251574 30.9684813027897 21.461947997677207 40-41 21.489635958227236 26.150311396149274 31.070325326434528 21.289727319188966 42-43 21.330948804546885 26.18603395239717 30.735092511304124 21.74792473175182 44-45 21.379979596857048 26.506721460922915 30.611587827571736 21.5017111146483 46-47 21.773210273628152 26.844584058437682 29.804456994829902 21.577748673104267 48-49 21.80965318394808 26.872478377696403 29.59065268923303 21.727215749122493 50-51 21.83140313173852 27.18038611267891 29.38175661008119 21.606454145501385 52-53 21.97664418921571 27.144619202079127 29.291838216610046 21.586898392095126 54-55 21.526783493499153 27.214109666478237 29.384465799886943 21.87464104013567 56-57 21.628336509615885 27.515242765196984 29.122328701160416 21.73409202402671 58-59 21.661147116696537 26.9939685179884 29.199843887861558 22.14504047745351 60-61 21.534021077268548 27.188193256829585 29.51780751550318 21.75997815039869 62-63 21.53009774423509 27.283770332478735 29.48467763125464 21.701454292031535 64-65 21.292397406957225 27.20587703304972 29.430158230595588 22.071567329397464 66-67 21.312402017285205 27.259034305876543 29.268180188262466 22.160383488575786 68-69 21.40667692782258 27.162543132360316 29.45913637130489 21.97164356851221 70-71 21.383770103984684 27.34580594801625 29.093190050068042 22.177233897931025 72-73 21.552393542087728 27.32266361068009 28.918689983940695 22.206252863291486 74-75 21.339272955515238 27.815547327350902 28.71015355228719 22.135026164846675 76-77 21.566256943938953 27.387930578469057 28.803641886029656 22.24217059156234 78-79 21.60295034516995 27.465165391767556 28.78982769307838 22.142056569984117 80-81 21.71007774930326 27.803288294561167 28.441659085853388 22.04497487028219 82-83 21.715657573791542 27.749643390805247 28.40783884655293 22.126860188850284 84-85 21.781778034575456 27.563161830461087 28.377906719147433 22.277153415816027 86-87 21.80316476842954 27.553261183593914 28.335840353668456 22.30773369430809 88-89 21.78935194294091 27.53198808992352 28.224646874735686 22.454013092399887 90-91 21.752538459832852 27.556277356969964 28.28477087003366 22.40641331316352 92-93 21.9479127696544 27.192328101328123 28.323262737377526 22.53649639163995 94-95 21.716319775702658 27.162578026152644 28.377974312381454 22.743127885763244 96-97 21.999443644086007 26.87095030565651 28.276359623034946 22.85324642722254 98-99 21.866754587581262 27.000720915318226 27.89033222708561 23.242192270014897 100-101 22.711237803150382 25.925620829716184 28.05064945763114 23.312491909502295 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2818.0 1 1828.5 2 1389.0 3 1728.5 4 1139.0 5 897.5 6 1624.0 7 3297.0 8 5018.0 9 5498.0 10 4282.0 11 2898.5 12 2582.5 13 2656.5 14 2719.5 15 2788.0 16 2876.5 17 2937.5 18 3095.5 19 3267.5 20 3509.0 21 3905.5 22 4347.0 23 4958.5 24 5714.0 25 6728.5 26 7914.5 27 9277.0 28 10617.5 29 11933.5 30 13529.0 31 15421.5 32 17820.0 33 19361.0 34 20192.5 35 21303.5 36 23035.0 37 24913.5 38 26091.0 39 26670.5 40 27459.5 41 29549.5 42 31053.0 43 32260.0 44 34020.5 45 36008.0 46 36986.0 47 37972.0 48 40036.5 49 40156.5 50 39355.0 51 38067.5 52 36806.5 53 36737.5 54 36620.5 55 34784.0 56 31677.5 57 29024.0 58 26477.0 59 23342.0 60 19701.5 61 16577.5 62 13978.5 63 10986.5 64 8359.5 65 6696.0 66 5161.5 67 3934.0 68 3009.5 69 2089.0 70 1539.5 71 1174.5 72 881.5 73 707.0 74 561.5 75 434.5 76 302.5 77 183.5 78 103.5 79 70.5 80 43.0 81 21.0 82 11.5 83 10.0 84 8.5 85 5.0 86 5.0 87 5.0 88 4.0 89 3.5 90 2.0 91 1.0 92 1.0 93 0.5 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14564120552225165 2 0.05098798256644937 3 0.1467260562151548 4 0.12629470149881164 5 0.19147614729741091 6 0.19518272049816346 7 0.1630892208331111 8 0.06915923167257759 9 0.11870074664848938 10-11 0.10025828486913536 12-13 0.14984500195725145 14-15 0.1343406774711769 16-17 0.13239698664639202 18-19 0.13126693384128452 20-21 0.13090531694365015 22-23 0.19414307191746458 24-25 0.2166085216830012 26-27 0.14609322664429464 28-29 0.12602348882558584 30-31 0.09899262572741498 32-33 0.12245252196144622 34-35 0.09230271312117873 36-37 0.08683325754445854 38-39 0.05216323748376114 40-41 0.05767789517268563 42-43 0.06179128738327685 44-45 0.03896422072010581 46-47 0.050174344546771985 48-49 0.04122432633032076 50-51 0.05053596144440637 52-53 0.02657884197612786 54-55 0.04682938824365385 56-57 0.026262427190697765 58-59 0.04768822837553553 60-61 0.03634249821225646 62-63 0.050038738210159084 64-65 0.03751775312956824 66-67 0.036116487651234964 68-69 0.06142967048564245 70-71 0.04949631286370749 72-73 0.050038738210159084 74-75 0.04167634745236375 76-77 0.021018982174999073 78-79 0.02156140752145066 80-81 0.025403587058816086 82-83 0.021742215970267858 84-85 0.019346504023440007 86-87 0.022465449765536643 88-89 0.06260492540295423 90-91 0.10771663338284472 92-93 0.07797364355241593 94-95 0.07453828302488918 96-97 0.06649230705252394 98-99 0.05460415154279329 100-101 0.13126693384128452 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1106143.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.149677912584856 #Duplication Level Percentage of deduplicated Percentage of total 1 83.28481419850097 51.76124377446308 2 10.105905435291437 12.561575356368069 3 2.794918191992042 5.211097962729882 4 1.1941963225474743 2.9687566724287526 5 0.6502787100996901 2.020730619305344 6 0.403633883416182 1.505142950935292 7 0.26817466600188705 1.166687838143261 8 0.18466146020065677 0.918132021947074 9 0.15247860366340232 0.8528846495617021 >10 0.8538579774396658 9.83521696572638 >50 0.07222545760793685 3.105940386266677 >100 0.030933897769901 3.76725846204897 >500 0.0027593597743236723 1.1422357789623463 >1k 7.261473090325454E-4 0.9000691574723444 >5k 2.9045892361301816E-4 1.3138193598242525 >10k+ 1.4522946180650908E-4 0.9692080438164895 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10738 0.9707605616995271 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8946 0.8087561915593191 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5610 0.5071676989322358 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2692 0.24336817210794626 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2295 0.20747769501773278 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1916 0.17321449396687408 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1760 0.15911143495913277 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1309 0.11833912975085499 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.040422440859816E-5 0.0 0.0 0.0 0.0 8 9.040422440859816E-5 0.0 0.0 0.0 0.0 9 9.040422440859816E-5 0.0 0.0 0.0 0.0 10-11 9.040422440859816E-5 0.0 0.0 4.520211220429908E-5 4.520211220429908E-5 12-13 9.040422440859816E-5 0.0 0.0 9.040422440859816E-5 9.040422440859816E-5 14-15 9.040422440859816E-5 0.0 0.0 1.3560633661289725E-4 1.3560633661289725E-4 16-17 9.040422440859816E-5 0.0 0.0 1.8080844881719632E-4 1.8080844881719632E-4 18-19 9.040422440859816E-5 0.0 0.0 1.8080844881719632E-4 1.8080844881719632E-4 20-21 9.040422440859816E-5 0.0 0.0 2.260105610214954E-4 2.712126732257945E-4 22-23 9.040422440859816E-5 0.0 0.0 8.136380196773835E-4 2.712126732257945E-4 24-25 9.040422440859816E-5 0.0 0.0 0.002124499273602057 2.712126732257945E-4 26-27 9.040422440859816E-5 0.0 0.0 0.0038421795373654224 2.712126732257945E-4 28-29 9.040422440859816E-5 0.0 0.0 0.008497997094408228 2.712126732257945E-4 30-31 9.040422440859816E-5 0.0 0.0 0.022781864550966736 2.712126732257945E-4 32-33 9.040422440859816E-5 0.0 0.0 0.04511170797989049 3.6161689763439264E-4 34-35 9.040422440859816E-5 0.0 0.0 0.07928450480634058 3.6161689763439264E-4 36-37 1.8080844881719632E-4 0.0 0.0 0.13470229436881126 3.6161689763439264E-4 38-39 1.8080844881719632E-4 0.0 0.0 0.21570447943891524 3.6161689763439264E-4 40-41 1.8080844881719632E-4 0.0 0.0 0.30335137500305115 3.6161689763439264E-4 42-43 2.260105610214954E-4 0.0 0.0 0.3889189734057893 3.6161689763439264E-4 44-45 2.712126732257945E-4 0.0 0.0 0.5002969778771822 3.6161689763439264E-4 46-47 2.712126732257945E-4 0.0 0.0 0.623427531521693 3.6161689763439264E-4 48-49 2.712126732257945E-4 0.0 0.0 0.7528411787626013 3.6161689763439264E-4 50-51 2.712126732257945E-4 0.0 0.0 0.889577568180606 3.6161689763439264E-4 52-53 2.712126732257945E-4 0.0 0.0 1.013657366181407 3.6161689763439264E-4 54-55 2.712126732257945E-4 0.0 0.0 1.152292244311992 3.6161689763439264E-4 56-57 2.712126732257945E-4 0.0 0.0 1.3085107440900499 3.6161689763439264E-4 58-59 2.712126732257945E-4 4.520211220429908E-5 0.0 1.475848963470365 3.6161689763439264E-4 60-61 2.712126732257945E-4 9.040422440859816E-5 0.0 1.6587819115611633 3.6161689763439264E-4 62-63 2.712126732257945E-4 9.040422440859816E-5 0.0 1.8388219244708868 3.6161689763439264E-4 64-65 2.712126732257945E-4 9.040422440859816E-5 0.0 2.01402531137475 3.6161689763439264E-4 66-67 2.712126732257945E-4 9.040422440859816E-5 0.0 2.2137282430933434 3.6161689763439264E-4 68-69 2.712126732257945E-4 9.040422440859816E-5 0.0 2.433591316855054 3.6161689763439264E-4 70-71 2.712126732257945E-4 9.040422440859816E-5 0.0 2.6714448312740755 3.6161689763439264E-4 72-73 2.712126732257945E-4 9.040422440859816E-5 0.0 2.906541016848635 4.068190098386917E-4 74-75 2.712126732257945E-4 9.040422440859816E-5 0.0 3.137569012324808 4.9722323424729E-4 76-77 2.712126732257945E-4 9.040422440859816E-5 0.0 3.3723939852261418 5.42425346451589E-4 78-79 2.712126732257945E-4 9.040422440859816E-5 0.0 3.6525114745561833 5.876274586558881E-4 80-81 2.712126732257945E-4 9.040422440859816E-5 0.0 3.9373299835554714 6.328295708601871E-4 82-83 2.712126732257945E-4 9.040422440859816E-5 0.0 4.224227789716158 7.232337952687853E-4 84-85 2.712126732257945E-4 9.040422440859816E-5 0.0 4.514741764853188 7.232337952687853E-4 86-87 2.712126732257945E-4 9.040422440859816E-5 0.0 4.810589589230325 7.684359074730843E-4 88-89 2.712126732257945E-4 9.040422440859816E-5 0.0 5.128722054924182 8.136380196773835E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4955 0.0 47.015984 1 GTATCAA 7925 0.0 37.45149 1 TATCAAC 10135 0.0 29.335016 2 CTTAGCG 65 0.0058022738 29.273636 3 ATCAACG 10115 0.0 29.25193 3 TCAACGC 10265 0.0 28.778141 4 CAACGCA 10450 0.0 28.223148 5 AACGCAG 10750 0.0 27.429308 6 GTACATG 7095 0.0 26.523144 1 TACATGG 7160 0.0 25.777971 2 ACGCAGA 11980 0.0 24.522596 7 CGCAGAG 11990 0.0 24.46139 8 ACATGGG 7325 0.0 24.158245 3 GAGTACT 7425 0.0 23.947987 12-13 GCAGAGT 12380 0.0 23.228983 9 GTACTTT 7805 0.0 22.504353 14-15 TAAGGTA 365 0.0 22.155733 4 TCGTATG 110 0.0028282483 21.654972 1 AGTACTT 7585 0.0 21.405678 12-13 CATGGGG 3215 0.0 21.15851 4 >>END_MODULE