FastQCFastQC Report
Thu 26 May 2016
SRR938168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938168_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106143
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104310.9430064648060874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91550.8276506744607162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57400.5189202481053535No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26910.24327776788353764No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24130.2181453934979474No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18280.16525892221891744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17460.15784577581741238No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12150.10984113265644677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47300.049.0181921
GTATCAA77550.038.0459481
TATCAAC100100.029.7044182
ATCAACG100850.029.2884983
CAACGCA103400.028.6108325
TCAACGC103600.028.5556014
AACGCAG105700.028.0331956
GTACATG71300.025.0551471
ACGCAGA120450.024.600327
TACATGG71350.024.5647962
CGCAGAG120150.024.5431798
ACATGGG70250.023.862233
GAGTACT75050.023.50664912-13
GTAAGGT4700.023.2376924
GCAGAGT125900.023.0828119
GTGGTAT23250.022.4785541
GTACTTT79650.022.14907814-15
TGGTATC23450.022.078242
AGTACTT75900.021.86694112-13
TAAGGTG5750.021.4718025
Produced by FastQC (version 0.11.5)