FastQCFastQC Report
Thu 26 May 2016
SRR938167_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938167_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235898
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96060.4296260383971004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84930.3798473812311653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52840.23632562844995614No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28560.12773391272768259No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26660.11923620844958044No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23010.10291167128375264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41300.061.795621
GTATCAA72950.043.5514761
ATCAACG91350.034.493883
TATCAAC93100.033.896552
TCAACGC93050.033.8636864
CAACGCA93950.033.48875
AACGCAG96700.032.5297786
TGGTATC14600.030.599342
ACGCAGA112000.027.782787
CGCAGAG111650.027.7422248
GTGGTAT17300.027.0230221
GCAGAGT121200.024.928569
GTACATG76200.024.7910061
TACATGG72300.024.585012
TAAGGTG11800.024.1660885
GAGTACT84500.023.16134512-13
CAGAGTA117650.022.89387310-11
ACATGGG75250.022.3580473
GTACTTT87950.021.95696414-15
GTATAGC8200.021.5249311
Produced by FastQC (version 0.11.5)