FastQCFastQC Report
Thu 26 May 2016
SRR938167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938167_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235898
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94040.42059163700669705No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86500.38686916845043917No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53010.23708594935904947No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26940.12048850171161654No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25450.11382451256721014No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24170.1080997433693308No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23300.10420868930514719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42650.060.884181
GTATCAA73250.041.2284431
TATCAAC93650.031.9428542
ATCAACG94050.031.6011893
TCAACGC94650.031.3987544
CAACGCA95750.031.0863085
TGGTATC17100.030.3152542
AACGCAG98500.030.2803866
GTGGTAT19300.028.5845581
CGCAGAG112950.026.0701458
ACGCAGA114300.025.9284367
TACATGG69200.023.7793982
TAAGGTG11450.023.6702585
GTACATG71650.023.4972971
GCAGAGT124600.023.4039069
GAGTACT83400.022.949712-13
CAGAGTA120800.022.29226110-11
ACATGGG69900.022.1099833
GTACTTT87500.021.68436414-15
GTAAGGT12000.021.3976234
Produced by FastQC (version 0.11.5)