##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938167_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2235898 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48614069157001 33.0 31.0 34.0 31.0 34.0 2 32.714294211989994 34.0 31.0 34.0 31.0 34.0 3 32.775123909945805 34.0 31.0 34.0 31.0 34.0 4 36.238990329612534 37.0 35.0 37.0 35.0 37.0 5 36.16115583090105 37.0 35.0 37.0 35.0 37.0 6 36.21474548481192 37.0 37.0 37.0 35.0 37.0 7 36.184120653088826 37.0 37.0 37.0 35.0 37.0 8 36.17578619418238 37.0 37.0 37.0 35.0 37.0 9 37.87146909206055 39.0 38.0 39.0 35.0 39.0 10-11 37.84560565821876 39.0 38.0 39.0 35.0 39.0 12-13 37.76178206698159 39.0 38.0 39.0 35.0 39.0 14-15 39.01861958819231 40.0 38.0 41.0 36.0 41.0 16-17 38.92036935495268 40.0 38.0 41.0 35.5 41.0 18-19 39.16600600742967 40.0 39.0 41.0 36.0 41.0 20-21 39.13566271806674 40.0 39.0 41.0 36.0 41.0 22-23 39.03180847247951 40.0 39.0 41.0 35.0 41.0 24-25 38.919635868899206 40.0 38.0 41.0 35.0 41.0 26-27 38.757050187441465 40.0 38.0 41.0 35.0 41.0 28-29 38.63547308508707 40.0 38.0 41.0 34.5 41.0 30-31 38.394905313211964 40.0 38.0 41.0 34.0 41.0 32-33 38.167299894717914 40.0 38.0 41.0 34.0 41.0 34-35 38.07179553807911 40.0 38.0 41.0 34.0 41.0 36-37 38.02007314287145 40.0 38.0 41.0 33.0 41.0 38-39 37.83193419377807 40.0 37.0 41.0 33.0 41.0 40-41 37.61123517262415 40.0 37.0 41.0 33.0 41.0 42-43 37.35573939419419 40.0 37.0 41.0 32.0 41.0 44-45 37.09256213834442 40.0 36.0 41.0 31.5 41.0 46-47 36.724783062554735 39.5 36.0 41.0 30.5 41.0 48-49 36.31500162350876 39.0 35.5 41.0 30.0 41.0 50-51 35.931530865898175 39.0 35.0 40.0 29.0 41.0 52-53 35.39327353036677 38.0 35.0 40.0 27.5 41.0 54-55 35.013437106701645 38.0 34.0 40.0 26.5 41.0 56-57 34.62251699317232 38.0 34.0 40.0 26.0 41.0 58-59 34.11330838884422 37.5 33.5 40.0 24.5 41.0 60-61 33.62513831131832 37.0 33.0 40.0 23.0 41.0 62-63 33.051523593652306 36.0 32.0 39.0 21.5 40.5 64-65 32.48174670758684 36.0 31.0 39.0 19.5 40.0 66-67 31.90458934173205 35.0 31.0 38.5 16.5 40.0 68-69 31.270945275678944 35.0 30.0 38.0 10.5 40.0 70-71 30.63302462813599 34.5 29.5 37.0 7.5 39.5 72-73 29.95483649075226 34.0 29.0 36.5 4.5 39.0 74-75 29.29596318794507 34.0 28.0 36.0 2.0 39.0 76-77 27.994495947489554 32.0 26.5 34.5 2.0 36.5 78-79 28.641876776132005 33.5 28.0 35.0 2.0 37.0 80-81 28.36685416776615 34.0 27.5 35.0 2.0 37.0 82-83 27.810278688920512 33.0 26.0 35.0 2.0 36.0 84-85 27.15677392260291 33.0 25.5 35.0 2.0 36.0 86-87 26.44117866736318 32.5 24.5 35.0 2.0 35.5 88-89 25.624564492655747 32.0 21.5 34.5 2.0 35.0 90-91 24.96763202078091 31.5 19.0 34.0 2.0 35.0 92-93 24.033626533947434 31.0 10.5 34.0 2.0 35.0 94-95 23.123886912551466 30.5 2.0 34.0 2.0 35.0 96-97 22.08015392473181 30.0 2.0 34.0 2.0 35.0 98-99 20.66787639686605 29.0 2.0 34.0 2.0 35.0 100-101 16.825392526850507 21.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06300441612587093 1101 2 0.13954269799723562 1101 3 0.10044831069355808 1101 4 0.05389926975449555 1101 5 -0.06457542733422628 1101 6 0.02154065065521138 1101 7 0.003987272963648536 1101 8 0.022612101485208314 1101 9 -0.0023622444310476 1101 10-11 0.09734387887338869 1101 12-13 0.16229769573033792 1101 14-15 0.17590281493590254 1101 16-17 0.28496869183268814 1101 18-19 0.20618684418305833 1101 20-21 0.17039359651418806 1101 22-23 0.2518402531269217 1101 24-25 0.13680259552634055 1101 26-27 0.2089613606531131 1101 28-29 0.2060296528967953 1101 30-31 0.27984682886187073 1101 32-33 0.3500972188029152 1101 34-35 0.39123661876026716 1101 36-37 0.44572185837807154 1101 38-39 0.41606222878458254 1101 40-41 0.607702781878416 1101 42-43 0.664472271729899 1101 44-45 0.6246757174871007 1101 46-47 0.6083068508472351 1101 48-49 0.6453514668772797 1101 50-51 0.656033159112674 1101 52-53 0.831533938982858 1101 54-55 0.9285681230045242 1101 56-57 0.8542124745275643 1101 58-59 0.9427056673511558 1101 60-61 0.8278429191894077 1101 62-63 0.8413460970880422 1101 64-65 1.0025722238466486 1101 66-67 1.3088396626855463 1101 68-69 1.587937419147675 1101 70-71 1.6103133830601202 1101 72-73 1.6530196105554786 1101 74-75 1.7679266243777505 1101 76-77 1.7564323635257146 1101 78-79 1.7286470956916524 1101 80-81 1.9789370682244432 1101 82-83 2.1831115233881953 1101 84-85 2.2539100594953574 1101 86-87 2.425634671486769 1101 88-89 2.7660199382292703 1101 90-91 2.888675696571699 1101 92-93 2.936008952958691 1101 94-95 3.3026901880233446 1101 96-97 3.4942758704121353 1101 98-99 3.730123103128097 1101 100-101 3.632469498540843 1102 1 0.20624996316951894 1102 2 0.110669338332265 1102 3 0.208109394494862 1102 4 0.09439737143446081 1102 5 -0.010389311165184267 1102 6 0.008715370828440427 1102 7 0.07700773739437494 1102 8 0.1667058017602514 1102 9 0.1467337979863359 1102 10-11 0.19692700183920664 1102 12-13 0.295655209936605 1102 14-15 0.3683116308690373 1102 16-17 0.3607189216421176 1102 18-19 0.3449027304697623 1102 20-21 0.2813859297193275 1102 22-23 0.3183180598852928 1102 24-25 0.31375342390349914 1102 26-27 0.2450555664177685 1102 28-29 0.37031529340742253 1102 30-31 0.3656886239818249 1102 32-33 0.250049312726766 1102 34-35 0.31657695847115974 1102 36-37 0.31010574127946455 1102 38-39 0.3799013433514915 1102 40-41 0.6305369989072247 1102 42-43 0.7045380947392701 1102 44-45 0.6273222471976823 1102 46-47 0.5631827902103552 1102 48-49 0.6293156901214161 1102 50-51 0.7048586248526973 1102 52-53 0.7756200751406155 1102 54-55 0.9946552689263726 1102 56-57 1.0423170343232755 1102 58-59 1.1326206029285757 1102 60-61 1.0135269614536853 1102 62-63 1.2269925921677114 1102 64-65 1.2252687982432349 1102 66-67 1.2451502508093348 1102 68-69 1.4139774548964787 1102 70-71 1.5743031724131278 1102 72-73 1.900416217578016 1102 74-75 1.872359136351232 1102 76-77 1.8003432794886756 1102 78-79 1.8845661845466424 1102 80-81 2.086755206187867 1102 82-83 2.129244527910263 1102 84-85 2.375428818810004 1102 86-87 2.482163385665814 1102 88-89 2.5867673702266387 1102 90-91 2.8172529620552034 1102 92-93 3.135161376908936 1102 94-95 3.248039744413692 1102 96-97 3.3355887399223008 1102 98-99 3.713362507743973 1102 100-101 3.5319609103818124 1103 1 0.21221660315538315 1103 2 0.206501264258236 1103 3 0.1979550582511962 1103 4 0.16284254066572146 1103 5 0.07635426372048926 1103 6 0.12750410090487208 1103 7 0.14210131104287882 1103 8 0.1354581933937169 1103 9 0.2566119850806956 1103 10-11 0.2754650800966161 1103 12-13 0.28448466323724375 1103 14-15 0.43606844123414845 1103 16-17 0.45287931256665104 1103 18-19 0.31964679332059376 1103 20-21 0.37876461312258414 1103 22-23 0.36261830461592837 1103 24-25 0.4545851743231353 1103 26-27 0.41347000765450304 1103 28-29 0.4816375236153405 1103 30-31 0.4947136891637314 1103 32-33 0.5248296564775075 1103 34-35 0.5517793287849173 1103 36-37 0.5977269629301603 1103 38-39 0.5905295179302357 1103 40-41 0.7250157997558659 1103 42-43 0.7910471816517415 1103 44-45 0.8857098676137269 1103 46-47 1.0024886712838068 1103 48-49 1.038294795660569 1103 50-51 1.2039884299140695 1103 52-53 1.2656736132044912 1103 54-55 1.252834439604264 1103 56-57 1.3280371770991124 1103 58-59 1.5009419394927548 1103 60-61 1.5159312543962002 1103 62-63 1.746223914697147 1103 64-65 1.760694464404871 1103 66-67 1.7112540792067144 1103 68-69 1.7731277222703135 1103 70-71 2.07088612159858 1103 72-73 2.0626587339590863 1103 74-75 2.208335230662005 1103 76-77 2.157923484445547 1103 78-79 2.3556926590156486 1103 80-81 2.524415355719267 1103 82-83 2.573766543597653 1103 84-85 2.6467580174826004 1103 86-87 3.0042543106072195 1103 88-89 3.1236757772102983 1103 90-91 3.3992465011203272 1103 92-93 3.25989157188209 1103 94-95 3.3180850423112425 1103 96-97 3.780303812579046 1103 98-99 4.5574845416149685 1103 100-101 4.419850052524108 1104 1 0.23746401571109743 1104 2 0.2281352010303621 1104 3 0.19477709748465344 1104 4 0.1019112595279239 1104 5 0.023412549307543884 1104 6 0.057013755527947296 1104 7 0.12103701624983643 1104 8 0.15891265185611303 1104 9 0.16098078651315006 1104 10-11 0.20678120753844098 1104 12-13 0.19930688537110797 1104 14-15 0.3541310488372389 1104 16-17 0.3576898582327743 1104 18-19 0.34329342974242394 1104 20-21 0.34444891907519803 1104 22-23 0.3406632318467544 1104 24-25 0.3946927144585075 1104 26-27 0.4470469431184938 1104 28-29 0.5172356410665557 1104 30-31 0.42786637521518855 1104 32-33 0.4496635550791126 1104 34-35 0.44847771144171844 1104 36-37 0.4895118190418799 1104 38-39 0.5367298945990342 1104 40-41 0.5769315489384468 1104 42-43 0.5681917882767138 1104 44-45 0.6440520477600202 1104 46-47 0.7405432206662539 1104 48-49 0.7393935234424731 1104 50-51 0.8138447735759726 1104 52-53 0.9227191885389132 1104 54-55 0.9561584556199847 1104 56-57 0.8830471239652269 1104 58-59 1.1737405409673514 1104 60-61 1.0824379494786953 1104 62-63 1.304039262161524 1104 64-65 1.255556023378979 1104 66-67 1.404573973336543 1104 68-69 1.5798359258835255 1104 70-71 1.5898942447621174 1104 72-73 1.7912252586791375 1104 74-75 1.80463732911279 1104 76-77 1.9305216906962386 1104 78-79 2.063422056544372 1104 80-81 2.1222648180848545 1104 82-83 2.258192441764539 1104 84-85 2.2733917620767663 1104 86-87 2.4299385635452246 1104 88-89 2.6812216779218865 1104 90-91 2.8840833950473694 1104 92-93 3.076030279306387 1104 94-95 3.4614509928830692 1104 96-97 3.686623154031569 1104 98-99 3.996380035240623 1104 100-101 3.711071363336835 1105 1 0.19911924548860327 1105 2 0.16771851156288164 1105 3 0.2271053708609685 1105 4 0.11082874976005286 1105 5 0.00963356035043006 1105 6 -0.01993553180975738 1105 7 0.10528219980552223 1105 8 0.11075295129466411 1105 9 0.12376328861994779 1105 10-11 0.19859735635297682 1105 12-13 0.22391826826794414 1105 14-15 0.34161248897324725 1105 16-17 0.2647121446620986 1105 18-19 0.3107440796568781 1105 20-21 0.3057550227623622 1105 22-23 0.3347609214856959 1105 24-25 0.25761895290761316 1105 26-27 0.2601305983993427 1105 28-29 0.3800816206443187 1105 30-31 0.36167396551554276 1105 32-33 0.4601318655933895 1105 34-35 0.45338262291983256 1105 36-37 0.5568293121925123 1105 38-39 0.5339795690033853 1105 40-41 0.5191608485338648 1105 42-43 0.5177976077745043 1105 44-45 0.5826631315101523 1105 46-47 0.7132676080539326 1105 48-49 0.8977293192639095 1105 50-51 0.8033137152168237 1105 52-53 0.8787862119927397 1105 54-55 1.0031952616087523 1105 56-57 1.0678741843381374 1105 58-59 1.189375698730764 1105 60-61 1.086223614514651 1105 62-63 1.1399558367128932 1105 64-65 1.343399358062122 1105 66-67 1.4881008399937237 1105 68-69 1.7025546586689124 1105 70-71 1.834353281728628 1105 72-73 2.092803439940152 1105 74-75 1.866346916034967 1105 76-77 1.7465552243119333 1105 78-79 1.8857584652517296 1105 80-81 2.0401687482158586 1105 82-83 2.1454925698139213 1105 84-85 2.3588415583286775 1105 86-87 2.4528827507964692 1105 88-89 2.465769291497306 1105 90-91 2.733785525190779 1105 92-93 2.9216942937821706 1105 94-95 2.9680475424812265 1105 96-97 3.2902840530038873 1105 98-99 3.1242809374233005 1105 100-101 3.2078110290951187 1106 1 0.08063096923950042 1106 2 0.08741593298071848 1106 3 0.0645418708638914 1106 4 0.03461893232437063 1106 5 -0.0856190658749938 1106 6 0.024466121382339168 1106 7 0.050663492077966055 1106 8 0.10725459104142487 1106 9 0.07062356816607718 1106 10-11 0.14350736026966615 1106 12-13 0.07737132357505061 1106 14-15 0.09081553112729779 1106 16-17 0.0673272046107698 1106 18-19 -0.04635780678261625 1106 20-21 9.304844343773766E-4 1106 22-23 0.018339953772049 1106 24-25 0.0330591123810251 1106 26-27 -0.05631024503261273 1106 28-29 -0.0074664756710731695 1106 30-31 0.1018948198206786 1106 32-33 0.1850707477596103 1106 34-35 0.10384625327823471 1106 36-37 0.1972468191928627 1106 38-39 0.01662420869753589 1106 40-41 0.17458592584456056 1106 42-43 0.05396767377358458 1106 44-45 -0.015975732534087683 1106 46-47 0.14957423192934982 1106 48-49 0.09145198284399214 1106 50-51 0.28648178779827305 1106 52-53 0.264729636345173 1106 54-55 0.3282754952069027 1106 56-57 0.3749002507581096 1106 58-59 0.511483694030737 1106 60-61 0.47905116927517355 1106 62-63 0.3671639413293235 1106 64-65 0.5372180096700063 1106 66-67 0.7583155562093857 1106 68-69 0.6574764126425912 1106 70-71 0.6268523025572073 1106 72-73 1.0730303738767688 1106 74-75 1.29707746559858 1106 76-77 1.2273643998788302 1106 78-79 1.4070425535700686 1106 80-81 1.2241122076430884 1106 82-83 1.671241329042978 1106 84-85 1.748930005275124 1106 86-87 1.9786573484593148 1106 88-89 2.1439906485412017 1106 90-91 2.062665996128384 1106 92-93 2.0715328912579523 1106 94-95 2.577782786221835 1106 96-97 2.8389678712462896 1106 98-99 3.0205881458786656 1106 100-101 2.775389991231876 1107 1 0.2326350418680434 1107 2 0.2755883409223472 1107 3 0.20617884128076724 1107 4 0.13509633489238837 1107 5 0.01617967839787582 1107 6 0.07390104417208931 1107 7 0.15487866261928218 1107 8 0.2131044694282096 1107 9 0.18183574080027398 1107 10-11 0.2784092212346536 1107 12-13 0.3044729695957571 1107 14-15 0.4676066392216498 1107 16-17 0.3759097036208914 1107 18-19 0.28683185326602967 1107 20-21 0.3418632294991468 1107 22-23 0.3209255072535768 1107 24-25 0.4294044939071284 1107 26-27 0.4610555562004919 1107 28-29 0.48394819710453874 1107 30-31 0.4842467628038776 1107 32-33 0.47440498612150606 1107 34-35 0.5280851808193887 1107 36-37 0.6739412023593303 1107 38-39 0.7012715303786194 1107 40-41 0.7861623724761628 1107 42-43 0.6394826915913328 1107 44-45 0.8293056747853313 1107 46-47 0.7795385964296031 1107 48-49 0.9811210817749227 1107 50-51 0.9357781280890194 1107 52-53 1.0341668217063358 1107 54-55 1.0119934656803409 1107 56-57 1.0486742198740089 1107 58-59 1.0495639021873089 1107 60-61 1.0640769060249937 1107 62-63 1.3317405576537809 1107 64-65 1.5052365627555915 1107 66-67 1.6011958096173835 1107 68-69 1.7940175065958748 1107 70-71 1.831621803094567 1107 72-73 1.9423001289534163 1107 74-75 1.8029782518421449 1107 76-77 1.8283242958005488 1107 78-79 1.9270176652845343 1107 80-81 2.167569982404107 1107 82-83 2.608473072919896 1107 84-85 2.7392377149872082 1107 86-87 2.917144253827267 1107 88-89 3.0834702571163497 1107 90-91 3.1858466058913812 1107 92-93 3.3313569763306354 1107 94-95 3.1808021877717003 1107 96-97 3.279496181671089 1107 98-99 3.2994246185298337 1107 100-101 2.999464882345624 1108 1 0.28613471673781987 1108 2 0.26287340303227325 1108 3 0.20287301552821901 1108 4 0.12859529469914577 1108 5 0.12692573493701076 1108 6 0.1727392902118723 1108 7 0.22028346650207453 1108 8 0.20443745863746443 1108 9 0.16671573311334953 1108 10-11 0.21867386159146918 1108 12-13 0.22903840121450258 1108 14-15 0.3394996345089396 1108 16-17 0.3352584823155951 1108 18-19 0.34336607685282416 1108 20-21 0.37995653628576775 1108 22-23 0.3928645347231665 1108 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-0.20388442451383781 2316 50-51 -0.053092889511788144 2316 52-53 0.03979144737530049 2316 54-55 0.3439009600291527 2316 56-57 0.4183939787565265 2316 58-59 0.5715736009836476 2316 60-61 0.7381118564683931 2316 62-63 0.681501951807455 2316 64-65 0.9504813511015797 2316 66-67 1.2247308470021885 2316 68-69 1.4876089592900819 2316 70-71 1.5739139648442126 2316 72-73 1.653554693376993 2316 74-75 2.0203332046039826 2316 76-77 1.8750354957850917 2316 78-79 1.9862295897807556 2316 80-81 2.0884170814593404 2316 82-83 2.3406723039714983 2316 84-85 2.6592081549107043 2316 86-87 2.822208590464374 2316 88-89 3.012824089300125 2316 90-91 3.355455755990878 2316 92-93 3.295773309721934 2316 94-95 3.722359694678712 2316 96-97 4.250238468036745 2316 98-99 4.494039564722765 2316 100-101 4.6434132777937585 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 22.0 5 42.0 6 77.0 7 224.0 8 524.0 9 1041.0 10 1807.0 11 3061.0 12 4589.0 13 7057.0 14 9505.0 15 13223.0 16 17139.0 17 23515.0 18 24821.0 19 20815.0 20 20373.0 21 21537.0 22 23500.0 23 25977.0 24 29264.0 25 32475.0 26 37010.0 27 42873.0 28 51173.0 29 61227.0 30 73403.0 31 87379.0 32 105916.0 33 132497.0 34 173179.0 35 235332.0 36 326192.0 37 404947.0 38 214341.0 39 9835.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1504031042561 18.605276269311034 14.524186702613445 23.72013392381942 2 16.0149529733729 19.563309479311954 39.5343053272987 24.887432220016443 3 19.341915706207512 23.02391557829041 29.940965809905347 27.69320290559673 4 13.1124122314054 16.36240518710773 35.839865894462555 34.68531668702431 5 13.676838688010747 37.049501847083846 34.5324974812493 14.741161983656106 6 33.61065665786185 35.43430872070193 16.43312888155005 14.521905739886169 7 29.290021280040502 32.46753653341968 19.842810360758854 18.39963182578096 8 26.908309118549056 35.146494040636114 18.996526220172434 18.948670620642403 9 26.300976162597756 16.388225223154187 19.773934231346868 37.536864382901186 10-11 25.438034293156488 26.218369532062734 26.625454291743182 21.718141883037596 12-13 26.02786441957549 23.439642595503017 27.35131477375086 23.181178211170636 14-15 22.7342660532815 25.068115808502895 25.462275112728754 26.735343025486852 16-17 22.314059566684072 28.214285314957415 26.616246943341544 22.85540817501697 18-19 22.37456717614131 26.5149394113685 28.98173351378283 22.128759898707365 20-21 22.69839679627604 25.66749467104492 29.567493687100217 22.066614845578826 22-23 22.508474257890263 25.599884430756624 29.426035907864833 22.465605403488276 24-25 22.377499618720233 25.913248901668283 29.38217918494238 22.327072294669108 26-27 21.99144002426027 26.297302833853813 29.13261029612381 22.5786468457621 28-29 22.15856895081976 26.70575759717125 29.044705974959502 22.09096747704949 30-31 22.192738666969603 26.208776071180353 29.04016641188462 22.558318849965428 32-33 21.909474403572972 26.040901686928475 29.164210531965235 22.88541337753332 34-35 22.61914320264962 26.255306367596386 28.882272835132856 22.243277594621137 36-37 22.624480126160673 25.934028630350745 28.89671131577335 22.54477992771523 38-39 22.244151410846964 26.199053866709843 28.955489512045613 22.601305210397584 40-41 22.61000136201055 26.0486866215004 28.96034760119526 22.380964415293793 42-43 22.259368319544997 26.031296201996053 28.791579979394093 22.917755499064864 44-45 22.38894254820449 26.389991830713967 28.929403100929484 22.291662520152062 46-47 22.762397926917956 26.617023674604116 28.213630496561116 22.406947901916812 48-49 22.529381930660524 26.623464039951735 28.171566860384505 22.675587169003236 50-51 22.70114736897658 26.875286797519387 28.080641424608814 22.342924408895218 52-53 22.911040664645704 26.525203743641256 28.016103596854595 22.54765199485844 54-55 22.49313251320051 26.791114800406817 28.094819173325437 22.620933513067232 56-57 22.561772747134594 27.0029209753401 27.881136742882163 22.554169534643144 58-59 22.55798341427024 26.6250070441496 27.923187909287456 22.893821632292706 60-61 22.38366866467075 26.85068817987225 28.103070891427066 22.662572264029933 62-63 22.295024191622336 26.86770595080813 28.334924043941186 22.50234581362835 64-65 22.18656665017814 26.802340715005784 28.07701871910078 22.9340739157153 66-67 22.13505362724669 26.97396168070354 27.925861522243643 22.965123169806123 68-69 22.51402694694471 26.60285123273886 28.14068317772684 22.74243864258959 70-71 22.356945620954086 26.799925578000426 27.809587020517036 23.03354178052845 72-73 22.31209666634912 26.83972025545005 27.779186608327667 23.068996469873166 74-75 22.40786475948366 27.22396102147772 27.371306741184082 22.996867477854536 76-77 22.44207920039286 26.812202524444317 27.820678760837925 22.925039514324894 78-79 22.375819469403343 26.90509584963178 27.830316946479673 22.888767734485207 80-81 22.545363794791037 26.996419556987544 27.591505241857124 22.866711406364292 82-83 22.449038921912642 27.11102742426421 27.60365919247212 22.836274461351024 84-85 22.356728223457033 27.16691985779552 27.550922054777395 22.925429863970056 86-87 22.55793504226408 27.062607370977044 27.42992177824452 22.94953580851436 88-89 22.33690892876151 27.23999484770772 27.190842337172803 23.232253886357963 90-91 22.407787494727934 27.04358225830907 27.38539994570276 23.163230301260242 92-93 22.416362463761764 26.792881428401476 27.43016452449978 23.360591583336987 94-95 22.191603148176515 26.730228018055392 27.569175241709427 23.50899359205867 96-97 22.46169375983806 26.436477017821268 27.458975609548837 23.642853612791832 98-99 22.089894082824888 26.65146173931011 27.222596916317293 24.03604726154771 100-101 22.56346219729165 25.980478537035236 27.456708669179005 23.999350596494114 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1024.0 1 722.0 2 553.5 3 702.0 4 564.5 5 496.5 6 1048.0 7 2284.0 8 3596.5 9 4191.5 10 3439.0 11 2408.0 12 2198.5 13 2268.5 14 2453.0 15 2575.0 16 2704.5 17 2918.0 18 3185.0 19 3533.5 20 3936.5 21 4742.5 22 5778.5 23 7129.0 24 8871.0 25 11151.5 26 13957.0 27 16762.5 28 19101.5 29 21738.5 30 25010.5 31 28927.0 32 34983.0 33 37761.5 34 38329.5 35 41175.5 36 45147.5 37 49782.0 38 53423.0 39 55650.0 40 58357.0 41 62818.5 42 65555.0 43 67230.0 44 70008.0 45 74318.5 46 77360.0 47 79674.5 48 80949.0 49 81365.0 50 82675.0 51 82659.0 52 84004.5 53 86657.5 54 88196.5 55 82658.5 56 74354.5 57 68016.0 58 61750.0 59 53748.0 60 43128.5 61 35361.5 62 29074.0 63 21435.5 64 15065.0 65 11139.0 66 8245.0 67 6285.5 68 4863.5 69 3731.5 70 2825.0 71 1957.5 72 1360.0 73 1060.5 74 799.0 75 564.0 76 388.0 77 233.5 78 131.5 79 80.5 80 43.5 81 19.5 82 16.0 83 11.5 84 5.0 85 2.5 86 1.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01592201433160189 3 0.0033543569518824203 4 0.0021020636898463167 5 0.11843116278112867 6 0.0 7 0.0 8 2.2362379679216133E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.2362379679216134E-5 18-19 0.0 20-21 0.0 22-23 4.0252283422589045E-4 24-25 3.130733155090259E-4 26-27 0.006440365347614247 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011561350294154742 36-37 8.497704278102133E-4 38-39 0.007446672433178972 40-41 0.010398506550835504 42-43 0.005881305855633843 44-45 0.003779242165787527 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.472475935843227E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.472475935843227E-5 68-69 0.0010286694652439422 70-71 0.0 72-73 4.472475935843227E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.708713903764841E-5 82-83 0.0022138755882423976 84-85 0.0046290125935977405 86-87 0.0033543569518824203 88-89 0.0 90-91 0.0025493112834306395 92-93 0.0 94-95 2.2362379679216134E-5 96-97 1.1181189839608066E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2235898.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.57497797137728 #Duplication Level Percentage of deduplicated Percentage of total 1 75.95656142004417 40.693711048604314 2 13.835353921574509 14.82457563149125 3 4.423446916839854 7.109582133817556 4 1.9038477791064183 4.079944113059277 5 1.0184112379320949 2.728067981900752 6 0.586621533906797 1.8856941453955323 7 0.40175475629176344 1.506680155275909 8 0.2786969421373883 1.194494602856063 9 0.2072069076145019 0.999099495986773 >10 1.2281744474156158 12.440151518267498 >50 0.10682156578469888 3.9374123193365063 >100 0.04734530246708011 4.5503391160178825 >500 0.0035044245387118025 1.326673498902801 >1k 0.0020025283078352555 1.679553093226462 >5k 2.5031603847940694E-4 1.0440211458613866 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9404 0.42059163700669705 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8650 0.38686916845043917 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5301 0.23708594935904947 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2694 0.12048850171161654 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2545 0.11382451256721014 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2417 0.1080997433693308 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2330 0.10420868930514719 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.944951871686455E-5 0.0 9 0.0 0.0 0.0 8.944951871686455E-5 0.0 10-11 0.0 0.0 0.0 8.944951871686455E-5 0.0 12-13 0.0 0.0 0.0 1.3417427807529682E-4 2.2362379679216135E-4 14-15 2.2362379679216137E-5 0.0 0.0 1.5653665775451295E-4 3.5779807486745815E-4 16-17 4.4724759358432275E-5 0.0 0.0 1.788990374337291E-4 4.0252283422589045E-4 18-19 4.4724759358432275E-5 0.0 0.0 1.788990374337291E-4 4.0252283422589045E-4 20-21 4.4724759358432275E-5 0.0 0.0 1.788990374337291E-4 4.472475935843227E-4 22-23 4.4724759358432275E-5 0.0 0.0 2.2362379679216138E-4 4.6960997326353884E-4 24-25 6.708713903764841E-5 0.0 0.0 3.130733155090259E-4 4.91972352942755E-4 26-27 8.944951871686455E-5 0.0 0.0 7.155961497349163E-4 4.91972352942755E-4 28-29 8.944951871686455E-5 0.0 0.0 0.003577980748674582 4.91972352942755E-4 30-31 8.944951871686455E-5 0.0 0.0 0.011360088877041798 4.91972352942755E-4 32-33 8.944951871686455E-5 0.0 0.0 0.030144487807583352 4.91972352942755E-4 34-35 1.3417427807529682E-4 0.0 0.0 0.05615193537451171 4.91972352942755E-4 36-37 1.788990374337291E-4 0.0 0.0 0.09993747478641692 5.366971123011873E-4 38-39 1.788990374337291E-4 0.0 0.0 0.1704907826743438 5.366971123011873E-4 40-41 2.2362379679216135E-4 0.0 0.0 0.24070865486708248 5.366971123011873E-4 42-43 2.2362379679216135E-4 0.0 0.0 0.30714728489403365 5.366971123011873E-4 44-45 2.2362379679216135E-4 0.0 0.0 0.3901340758836047 5.814218716596195E-4 46-47 2.2362379679216135E-4 0.0 0.0 0.4905858854026436 6.708713903764841E-4 48-49 2.6834855615059364E-4 0.0 0.0 0.5960468679698269 7.155961497349164E-4 50-51 2.6834855615059364E-4 0.0 0.0 0.7040124370610824 8.944951871686454E-4 52-53 2.6834855615059364E-4 0.0 0.0 0.807326631179061 8.944951871686454E-4 54-55 2.6834855615059364E-4 0.0 0.0 0.9213300427837048 9.392199465270777E-4 56-57 2.6834855615059364E-4 0.0 0.0 1.0529102848162126 9.8394470588551E-4 58-59 2.6834855615059364E-4 0.0 0.0 1.1893878879984685 9.8394470588551E-4 60-61 2.6834855615059364E-4 0.0 0.0 1.3366217958064277 9.8394470588551E-4 62-63 2.6834855615059364E-4 0.0 0.0 1.4770799025715842 9.8394470588551E-4 64-65 2.6834855615059364E-4 0.0 0.0 1.6229944299784695 0.0010733942246023745 66-67 2.6834855615059364E-4 0.0 0.0 1.7955425515833012 0.0010733942246023745 68-69 3.130733155090259E-4 0.0 0.0 1.9843033984555645 0.0010733942246023745 70-71 3.130733155090259E-4 0.0 0.0 2.176060804204843 0.001140481363640023 72-73 3.130733155090259E-4 0.0 0.0 2.3708371312108154 0.001297018021394536 74-75 3.130733155090259E-4 0.0 0.0 2.5686547418531616 0.0013864675401114004 76-77 3.130733155090259E-4 0.0 0.0 2.779330720811057 0.0013864675401114004 78-79 3.577980748674582E-4 0.0 0.0 3.01601414733588 0.0014088299197906166 80-81 3.577980748674582E-4 0.0 0.0 3.2617543376307863 0.0014535546791490488 82-83 3.577980748674582E-4 0.0 0.0 3.513018035706459 0.0016771784759412101 84-85 3.577980748674582E-4 0.0 0.0 3.7621349453329267 0.0016995408556204263 86-87 3.577980748674582E-4 0.0 0.0 4.026614809799016 0.0017219032352996425 88-89 3.577980748674582E-4 0.0 0.0 4.306122193409538 0.0017442656149788587 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4265 0.0 60.88418 1 GTATCAA 7325 0.0 41.228443 1 TATCAAC 9365 0.0 31.942854 2 ATCAACG 9405 0.0 31.601189 3 TCAACGC 9465 0.0 31.398754 4 CAACGCA 9575 0.0 31.086308 5 TGGTATC 1710 0.0 30.315254 2 AACGCAG 9850 0.0 30.280386 6 GTGGTAT 1930 0.0 28.584558 1 CGCAGAG 11295 0.0 26.070145 8 ACGCAGA 11430 0.0 25.928436 7 TACATGG 6920 0.0 23.779398 2 TAAGGTG 1145 0.0 23.670258 5 GTACATG 7165 0.0 23.497297 1 GCAGAGT 12460 0.0 23.403906 9 GAGTACT 8340 0.0 22.9497 12-13 CAGAGTA 12080 0.0 22.292261 10-11 ACATGGG 6990 0.0 22.109983 3 GTACTTT 8750 0.0 21.684364 14-15 GTAAGGT 1200 0.0 21.397623 4 >>END_MODULE