FastQCFastQC Report
Thu 26 May 2016
SRR938166_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938166_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225356
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97590.43853657572091836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85880.3859157815648373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52580.23627680245318053No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28420.12770990349409264No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25960.11665549242458285No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23850.1071738634178082No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22530.10124222820977857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39450.059.9275131
GTATCAA70100.044.081291
TATCAAC89050.034.3750722
ATCAACG89950.033.925443
TCAACGC90600.033.7869764
CAACGCA91850.033.1719175
AACGCAG94700.032.2172746
CGCGTAA452.4135763E-531.66845910-11
TGGTATC15250.030.2338562
GTGGTAT17650.027.8715131
ACGCAGA111050.027.3350587
CGCAGAG110300.027.305588
GCAGAGT122000.023.9460269
TACATGG71350.023.6497232
GTACATG74400.023.0456831
GAGTACT82000.022.21619812-13
CAGAGTA118650.021.67948310-11
ACATGGG72950.021.567233
GTATAGG5700.020.9475481
TAAGGTG11200.020.7955175
Produced by FastQC (version 0.11.5)