##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938166_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2225356 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86728505461598 33.0 31.0 34.0 30.0 34.0 2 32.062411137813456 34.0 31.0 34.0 30.0 34.0 3 32.14434319722328 34.0 31.0 34.0 30.0 34.0 4 35.66020717584063 37.0 35.0 37.0 35.0 37.0 5 35.663615619253726 37.0 35.0 37.0 35.0 37.0 6 35.68447924736537 37.0 35.0 37.0 33.0 37.0 7 35.62266711483466 37.0 35.0 37.0 33.0 37.0 8 35.59348571644267 37.0 35.0 37.0 33.0 37.0 9 37.2356117403238 39.0 37.0 39.0 34.0 39.0 10-11 37.15284385958921 39.0 37.0 39.0 34.0 39.0 12-13 37.04791121061079 39.0 37.0 39.0 33.0 39.0 14-15 38.24610916185995 40.0 38.0 41.0 33.0 41.0 16-17 38.092132674502416 40.0 38.0 41.0 33.0 41.0 18-19 38.234924883928684 40.0 38.0 41.0 33.5 41.0 20-21 38.21061461626814 40.0 38.0 41.0 33.5 41.0 22-23 38.12728570170346 40.0 38.0 41.0 33.0 41.0 24-25 38.02161901286806 40.0 38.0 41.0 33.0 41.0 26-27 37.8440507945695 40.0 38.0 41.0 33.0 41.0 28-29 37.631852611447336 40.0 37.5 41.0 32.0 41.0 30-31 37.41646370288619 40.0 37.0 41.0 32.0 41.0 32-33 37.166943401415324 40.0 36.5 41.0 31.0 41.0 34-35 36.935458641224145 40.0 36.0 41.0 30.5 41.0 36-37 36.68264628221283 39.0 36.0 41.0 30.0 41.0 38-39 36.39807922867173 39.0 35.0 41.0 30.0 41.0 40-41 36.18565074531895 39.0 35.0 40.5 30.0 41.0 42-43 36.024512931863484 39.0 35.0 41.0 29.0 41.0 44-45 35.76900796996076 39.0 35.0 40.5 27.5 41.0 46-47 35.39342064820191 39.0 35.0 40.0 26.5 41.0 48-49 35.01204279225436 38.0 34.5 40.0 24.5 41.0 50-51 34.30936757983891 37.5 33.5 39.5 24.0 40.5 52-53 34.43122785747539 38.0 34.0 40.0 24.0 40.5 54-55 34.54681947517611 38.0 34.0 40.0 24.0 41.0 56-57 34.24497248979489 38.0 34.0 40.0 23.0 41.0 58-59 33.922546774538546 38.0 33.5 40.0 22.0 41.0 60-61 33.49769407681288 37.0 33.0 40.0 20.5 41.0 62-63 32.96056990432093 36.5 32.5 39.5 18.0 41.0 64-65 32.436246605037574 36.0 32.0 39.0 14.0 40.5 66-67 31.901645174974252 35.5 31.0 39.0 9.0 40.0 68-69 31.294512653256376 35.0 30.5 38.0 7.0 40.0 70-71 30.741318467696853 35.0 30.0 37.5 2.0 40.0 72-73 30.100820273250662 34.5 29.0 37.0 2.0 39.0 74-75 29.425236456548973 34.0 28.5 36.0 2.0 39.0 76-77 28.760294083283753 34.0 27.5 36.0 2.0 38.5 78-79 28.040326806137983 34.0 26.0 35.0 2.0 37.0 80-81 27.381299890893864 33.0 25.5 35.0 2.0 37.0 82-83 26.654955431850006 32.5 24.5 35.0 2.0 36.0 84-85 26.012421832731484 32.0 22.5 35.0 2.0 36.0 86-87 25.33785493197493 32.0 19.5 34.5 2.0 35.0 88-89 24.535838760180393 31.0 16.0 34.0 2.0 35.0 90-91 23.723706454158346 31.0 7.0 34.0 2.0 35.0 92-93 22.847497434118406 30.0 2.0 34.0 2.0 35.0 94-95 21.910845500674945 29.5 2.0 34.0 2.0 35.0 96-97 20.7315550860177 29.0 2.0 34.0 2.0 35.0 98-99 19.264632040895926 26.5 2.0 34.0 2.0 35.0 100-101 15.580034385509554 12.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2857723352761461 1101 2 0.27153997095248883 1101 3 0.2664414898634604 1101 4 0.10078223370725681 1101 5 0.19916278990684333 1101 6 0.23216409296821183 1101 7 0.21238485835873888 1101 8 0.16485908964315854 1101 9 0.30835426928762644 1101 10-11 0.25478753124125575 1101 12-13 0.41400509038822264 1101 14-15 0.5009267039828273 1101 16-17 0.5494665389443014 1101 18-19 0.46072465253585193 1101 20-21 0.5015268446670405 1101 22-23 0.6265523009386058 1101 24-25 0.6326978684599354 1101 26-27 0.6842156021507009 1101 28-29 0.69583450759432 1101 30-31 0.7569844217381032 1101 32-33 0.7107233946909091 1101 34-35 0.6746757835973085 1101 36-37 0.7514078717889916 1101 38-39 0.8257665674105326 1101 40-41 0.8418359646848401 1101 42-43 0.8796099580641226 1101 44-45 0.8285132202554735 1101 46-47 0.9773992024862608 1101 48-49 1.0559284947004315 1101 50-51 0.9605623145999189 1101 52-53 1.0775546387968902 1101 54-55 1.1690278598087218 1101 56-57 1.2041864219308351 1101 58-59 1.3373746251164178 1101 60-61 1.3820169351755496 1101 62-63 1.2400055897049782 1101 64-65 1.37599199763946 1101 66-67 1.3075326520933963 1101 68-69 1.61351022789907 1101 70-71 1.7264770280568662 1101 72-73 1.8922228180153873 1101 74-75 2.1552993718762785 1101 76-77 2.0774234696629357 1101 78-79 2.143304067420999 1101 80-81 2.39465953850209 1101 82-83 2.6054196851954075 1101 84-85 2.715201931106172 1101 86-87 2.9741742478921616 1101 88-89 3.3783559817865054 1101 90-91 3.5447500491298136 1101 92-93 3.6703337956347504 1101 94-95 3.7449961174848596 1101 96-97 3.9596558604097396 1101 98-99 4.228888408062762 1101 100-101 4.203419314425872 1102 1 0.4009766290518719 1102 2 0.29619806703915685 1102 3 0.27805985862468674 1102 4 0.2286019686698495 1102 5 0.2530259151985419 1102 6 0.3802682410723577 1102 7 0.20926989095805482 1102 8 0.34472459093723273 1102 9 0.5742225708702122 1102 10-11 0.43794125010926166 1102 12-13 0.45544370325541195 1102 14-15 0.4798096292943228 1102 16-17 0.5628014987078274 1102 18-19 0.3708608483863358 1102 20-21 0.5300318017434336 1102 22-23 0.6809740696460835 1102 24-25 0.4768416590322957 1102 26-27 0.4871488336553611 1102 28-29 0.41511619116172227 1102 30-31 0.42786389618900955 1102 32-33 0.636383159636388 1102 34-35 0.660612465962565 1102 36-37 0.6862658066469223 1102 38-39 0.48399170777852873 1102 40-41 0.5720872127932282 1102 42-43 0.7334221690191924 1102 44-45 0.7365438961432886 1102 46-47 0.7320062428075857 1102 48-49 0.689642271271353 1102 50-51 0.7454527780618108 1102 52-53 0.7210676573099093 1102 54-55 0.6838336781859695 1102 56-57 0.8338288194303729 1102 58-59 0.7532899517460336 1102 60-61 0.8709801919959546 1102 62-63 0.9018901980181582 1102 64-65 0.7741102886148923 1102 66-67 0.7796888742496186 1102 68-69 0.9593228772831424 1102 70-71 1.077660432811701 1102 72-73 1.1830426802638208 1102 74-75 1.1482880434363771 1102 76-77 1.3827044178010262 1102 78-79 1.6611117102286457 1102 80-81 1.663572093128927 1102 82-83 1.9840172780787135 1102 84-85 2.235334308381404 1102 86-87 2.2201382259989977 1102 88-89 2.461609761468857 1102 90-91 2.561297208534114 1102 92-93 2.8334753516334494 1102 94-95 3.2358014832902278 1102 96-97 3.645167903064639 1102 98-99 3.848270152444506 1102 100-101 3.740970405548392 1103 1 0.5286091059209888 1103 2 0.37777906083388757 1103 3 0.4422137365190224 1103 4 0.2635522154521581 1103 5 0.3135947047749639 1103 6 0.32304013804272813 1103 7 0.35442311817235606 1103 8 0.3286996111717926 1103 9 0.360327377967387 1103 10-11 0.5253664615802762 1103 12-13 0.4975950736510555 1103 14-15 0.7009079295910965 1103 16-17 0.6865755222910153 1103 18-19 0.6479632590765405 1103 20-21 0.5810943146380083 1103 22-23 0.6660004936038035 1103 24-25 0.6355619618365651 1103 26-27 0.7256151331903666 1103 28-29 0.8057186256572848 1103 30-31 0.8002294355392792 1103 32-33 0.9400186359817866 1103 34-35 0.846974767782875 1103 36-37 0.8752090070405032 1103 38-39 0.9198342590790958 1103 40-41 0.9143230922428458 1103 42-43 0.9760544078346314 1103 44-45 0.999495999744255 1103 46-47 1.0685189151981191 1103 48-49 0.9679984313069028 1103 50-51 0.860404929273507 1103 52-53 0.9845395114306257 1103 54-55 1.0561198663118532 1103 56-57 1.1141032953155374 1103 58-59 1.1297777872033308 1103 60-61 1.1038343761860148 1103 62-63 1.2247643787167704 1103 64-65 1.3289142117394306 1103 66-67 1.4180099610439818 1103 68-69 1.5909050613462483 1103 70-71 1.7750208143323896 1103 72-73 1.9094872693038347 1103 74-75 1.9526198267757948 1103 76-77 2.286308550412791 1103 78-79 2.4447189375381626 1103 80-81 2.5624786332568448 1103 82-83 2.6557730032695552 1103 84-85 2.7869840203364618 1103 86-87 2.8577590382381324 1103 88-89 3.095687541539 1103 90-91 3.2729427942255 1103 92-93 3.65497873993073 1103 94-95 4.050073991265027 1103 96-97 4.460946100522229 1103 98-99 4.644303781493399 1103 100-101 4.697856901557024 1104 1 0.22154998275775384 1104 2 0.21750909075981895 1104 3 0.14814727690487928 1104 4 0.14970634459350407 1104 5 0.12039560184533826 1104 6 0.02043806798917558 1104 7 0.11744938648695324 1104 8 0.06530545910846541 1104 9 0.21246994706939404 1104 10-11 0.07678352148165146 1104 12-13 0.19868068444419862 1104 14-15 0.24214274554310578 1104 16-17 0.3010758017411703 1104 18-19 0.3158862723274254 1104 20-21 0.31988977834839716 1104 22-23 0.34177110393709853 1104 24-25 0.4758491234012041 1104 26-27 0.474775014052625 1104 28-29 0.46764230392880535 1104 30-31 0.4109093837525677 1104 32-33 0.35758084017142266 1104 34-35 0.42787381906728683 1104 36-37 0.49105878312664686 1104 38-39 0.6124107895108679 1104 40-41 0.7528946420946383 1104 42-43 0.6427977169489552 1104 44-45 0.5254406473894448 1104 46-47 0.6203162395513218 1104 48-49 0.6062254438786212 1104 50-51 0.5383916854585493 1104 52-53 0.5795295714947244 1104 54-55 0.7678422697246816 1104 56-57 0.8704086105282371 1104 58-59 0.8129078411831969 1104 60-61 0.9235094357902582 1104 62-63 0.9338673790917298 1104 64-65 1.1492136125133925 1104 66-67 1.1398509362189095 1104 68-69 1.307452401617489 1104 70-71 1.5278489206507864 1104 72-73 1.6423205401440946 1104 74-75 1.741348434689538 1104 76-77 1.5321733955832535 1104 78-79 1.865952904527667 1104 80-81 2.076479439771216 1104 82-83 2.331792996336361 1104 84-85 2.239511098702774 1104 86-87 2.3972322570086924 1104 88-89 2.502973762170207 1104 90-91 2.5996147197431902 1104 92-93 2.9040236571215132 1104 94-95 3.0781198210904925 1104 96-97 3.269237987978098 1104 98-99 3.3878870399529575 1104 100-101 3.116865879335432 1105 1 0.39362127124092794 1105 2 0.33825026467221875 1105 3 0.14509796734844826 1105 4 0.11896160287913915 1105 5 0.15937563270998822 1105 6 0.27080002044240814 1105 7 0.22051284058369447 1105 8 0.3288117006507676 1105 9 0.360665251504237 1105 10-11 0.3062198932170688 1105 12-13 0.25203631829905504 1105 14-15 0.37505645751382133 1105 16-17 0.42978668522604835 1105 18-19 0.323226127630889 1105 20-21 0.3505215748527917 1105 22-23 0.3161414080024869 1105 24-25 0.37177876803546184 1105 26-27 0.31822873079448044 1105 28-29 0.41950790546231786 1105 30-31 0.4154837703122922 1105 32-33 0.5139932780779404 1105 34-35 0.509547983747737 1105 36-37 0.599991482559382 1105 38-39 0.6375678044639628 1105 40-41 0.7214387677826011 1105 42-43 0.6033230493984334 1105 44-45 0.7380450161523839 1105 46-47 0.8042210193503863 1105 48-49 0.9129233059419093 1105 50-51 0.9002051210829478 1105 52-53 0.7507913489356568 1105 54-55 0.8743769417930096 1105 56-57 0.8732132406485107 1105 58-59 0.9266786067406088 1105 60-61 0.9718661357634417 1105 62-63 1.0437848129765612 1105 64-65 1.0608773693455262 1105 66-67 1.2232401791904444 1105 68-69 1.2417585063588632 1105 70-71 1.388409086669693 1105 72-73 1.6256948996814167 1105 74-75 1.7275228854274864 1105 76-77 1.7394839062433824 1105 78-79 1.846839408313972 1105 80-81 2.10274162888161 1105 82-83 2.1908334687217845 1105 84-85 2.3221811868000373 1105 86-87 2.6315652785991723 1105 88-89 2.9421442165477316 1105 90-91 2.927039611178568 1105 92-93 3.0683417319440203 1105 94-95 3.4339712274166914 1105 96-97 3.558245252360667 1105 98-99 3.5078824588107977 1105 100-101 3.865922100716489 1106 1 0.035781771203406976 1106 2 0.09903258180599295 1106 3 0.00174881917919123 1106 4 0.1619484770386208 1106 5 0.17069983955100554 1106 6 0.02492550193620957 1106 7 -0.004572504763231677 1106 8 0.04192293662177349 1106 9 -0.04092461618258625 1106 10-11 0.1149285669006801 1106 12-13 0.1719626058829462 1106 14-15 0.22976774378939524 1106 16-17 0.03192817898766975 1106 18-19 0.05548058354035845 1106 20-21 -0.008642172593944508 1106 22-23 0.10930820212090708 1106 24-25 -0.04695694435223885 1106 26-27 0.03598918369784343 1106 28-29 0.16329174187512052 1106 30-31 0.002400424977537341 1106 32-33 0.07335935941312499 1106 34-35 0.28886611347266467 1106 36-37 0.3183245555801193 1106 38-39 0.11890075375168863 1106 40-41 0.4287987517175935 1106 42-43 0.3054481130397946 1106 44-45 0.34043499012788914 1106 46-47 0.49421274343795574 1106 48-49 0.6190621871115738 1106 50-51 0.49701341809083743 1106 52-53 0.597500558119485 1106 54-55 0.26273092771990747 1106 56-57 0.5838600600003545 1106 58-59 0.756446637783398 1106 60-61 0.6728545799121903 1106 62-63 0.6431361274876011 1106 64-65 0.7696375646408313 1106 66-67 0.9023532366735516 1106 68-69 0.5753769665117652 1106 70-71 0.7924490706858229 1106 72-73 1.252950745051674 1106 74-75 1.4472515044657364 1106 76-77 1.4518937180677973 1106 78-79 1.7301503615246432 1106 80-81 1.8584378095885938 1106 82-83 2.0985285459737746 1106 84-85 2.106825413844014 1106 86-87 1.7933270134575992 1106 88-89 2.1874658919331793 1106 90-91 2.573170013594808 1106 92-93 2.7677294564129333 1106 94-95 2.8214804820369004 1106 96-97 3.0891658259138524 1106 98-99 3.4761077218448513 1106 100-101 3.2777970208478493 1107 1 0.2417000555480051 1107 2 0.1129313041339941 1107 3 0.09298347789613359 1107 4 0.07999247470951332 1107 5 0.1831242807252309 1107 6 0.23956105817835294 1107 7 0.1644297181583525 1107 8 0.21351051816052546 1107 9 0.14575326411851108 1107 10-11 0.23741129699644148 1107 12-13 0.24729066793922527 1107 14-15 0.2641555625317835 1107 16-17 0.30139417181406714 1107 18-19 0.27870010921741795 1107 20-21 0.4817095465765817 1107 22-23 0.3683974942627941 1107 24-25 0.27883360345858677 1107 26-27 0.29019717157240166 1107 28-29 0.44019555212141626 1107 30-31 0.49957884021346644 1107 32-33 0.42691888974378855 1107 34-35 0.47161200237105305 1107 36-37 0.6363764689831584 1107 38-39 0.5331300585204062 1107 40-41 0.620103502038539 1107 42-43 0.6270417895006304 1107 44-45 0.737351937039584 1107 46-47 0.8083851814336214 1107 48-49 0.8493092729529863 1107 50-51 0.6811739805716499 1107 52-53 0.5631683219999033 1107 54-55 0.7633652404446067 1107 56-57 0.8593065513760365 1107 58-59 0.8117144521098325 1107 60-61 0.861678716110319 1107 62-63 1.05533014994068 1107 64-65 1.2523982308503179 1107 66-67 1.3061607996668734 1107 68-69 1.3945638195731576 1107 70-71 1.5946471909198543 1107 72-73 1.6494305058853982 1107 74-75 1.7213513630558737 1107 76-77 1.9819085260451672 1107 78-79 2.0612452165287465 1107 80-81 2.1851577371855164 1107 82-83 2.373997078734366 1107 84-85 2.293257293123162 1107 86-87 2.2927839328999795 1107 88-89 2.6421816749830853 1107 90-91 2.805494931207946 1107 92-93 3.0767662517258962 1107 94-95 3.519376852261633 1107 96-97 3.7070537897460483 1107 98-99 3.9510141801239627 1107 100-101 3.8962336234999455 1108 1 0.20268734952043488 1108 2 0.112908078855817 1108 3 0.1273878566352451 1108 4 0.10329427027557614 1108 5 0.07757457349877228 1108 6 0.0687031290053639 1108 7 0.10326621814133574 1108 8 0.08707984552586367 1108 9 -0.019873576237223745 1108 10-11 0.11918679929709697 1108 12-13 0.19188649678728353 1108 14-15 0.2250993766229712 1108 16-17 0.30917770069883943 1108 18-19 0.16586828705751344 1108 20-21 0.22714528430233827 1108 22-23 0.19115648447090905 1108 24-25 0.17455912604085455 1108 26-27 0.21757460736846213 1108 28-29 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-2.55828928430876 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5846.0 3 1976.0 4 1992.0 5 2218.0 6 2656.0 7 3128.0 8 3944.0 9 5017.0 10 6348.0 11 7868.0 12 10019.0 13 12055.0 14 14401.0 15 16858.0 16 20719.0 17 24685.0 18 24592.0 19 21266.0 20 21490.0 21 22798.0 22 24646.0 23 27710.0 24 30829.0 25 34514.0 26 39119.0 27 45004.0 28 51777.0 29 60857.0 30 71607.0 31 85303.0 32 103705.0 33 131569.0 34 173973.0 35 236695.0 36 313789.0 37 360490.0 38 191878.0 39 12015.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.94801341560148 18.17268636629793 14.139744193427367 23.739556024673224 2 15.744190949618858 19.397276605156296 40.10253076754673 24.756001677678118 3 19.306700332204404 23.027398658324017 29.69212308242886 27.973777927042715 4 12.876086817729348 16.03495081725456 36.06787119956621 35.021091165449874 5 13.726197420252559 36.97522566381432 34.418279589719326 14.88029732621379 6 33.513511568719956 35.24912313208809 16.63600233681532 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26.657082122059535 29.027526119794672 22.09990539083431 30-31 22.191509482099583 26.215020800220106 29.05363775135988 22.539831966320435 32-33 21.96221736244435 26.08844301213618 29.115172015232098 22.83416761018737 34-35 22.667900613204292 26.333616493642154 28.820759074935726 22.177723818217824 36-37 22.68892942912297 25.91842661533302 28.848787224611378 22.543856730932628 38-39 22.264410963639047 26.222176874631565 28.89352952558904 22.619882636140346 40-41 22.64899563880123 26.082287905640257 28.921404483094793 22.347311972463725 42-43 22.295857780718308 26.086367326098074 28.756372351164977 22.861402542018638 44-45 22.394359202419594 26.396845489525976 28.892930328904953 22.31586497914948 46-47 22.78664963091068 26.61463793961746 28.222067147398622 22.376645282073238 48-49 22.59101246372323 26.63488462566241 28.144793776701192 22.62930913391316 50-51 22.74502154618362 26.895515840984313 28.01367917489892 22.345783437933147 52-53 22.868837907500918 26.564082166476776 28.04644504819112 22.520634877831185 54-55 22.42882096246504 26.807237105706033 28.12026756787792 22.64367436395101 56-57 22.574508931049337 26.998831695107256 27.937048613303666 22.489610760539744 58-59 22.540151155440494 26.613928025777277 28.023148134623753 22.822772684158476 60-61 22.39459797264425 26.81254273844711 28.211453712815164 22.58140557609348 62-63 22.326277504306194 26.853551191745588 28.410118236353604 22.41005306759461 64-65 22.226963837877197 26.81235336946849 28.187354493074096 22.77332829958022 66-67 22.18235232258082 26.912339550431113 28.08091035496869 22.824397772019374 68-69 22.496640615520132 26.564214802863678 28.244626108372174 22.694518473244017 70-71 22.36627377835706 26.724480050337647 28.013078798638187 22.896167372667108 72-73 22.32275580877715 26.750700500423257 28.01932030616402 22.907223384635575 74-75 22.437213871811426 27.165799242137396 27.62688513435624 22.770101751694945 76-77 22.640937079596444 26.85405622091091 27.744325879141286 22.760680820351357 78-79 22.609905204057902 26.86786881598891 27.79578367604214 22.72644230391105 80-81 22.759770809002333 26.988864726119367 27.54312275318641 22.708241711691894 82-83 22.712612909807884 27.06325286313835 27.508892110852734 22.715242116201033 84-85 22.645703936073634 27.16187428653609 27.501213450423812 22.691208326966464 86-87 22.91451338346027 27.02135997023301 27.347048736251196 22.717077910055526 88-89 22.710050062085976 27.071710707443014 27.257845026660448 22.960394203810562 90-91 22.801669635636994 27.01007831698714 27.229997882838724 22.958254164537145 92-93 22.936634367783004 26.680432961895047 27.38555671585544 22.997375954466506 94-95 22.754071522871595 26.58360225095172 27.517902743746347 23.14442348243034 96-97 23.069805679313333 26.203154834464232 27.33529128325728 23.39174820296515 98-99 22.79411500311932 26.453472692916897 27.107361235214693 23.64505106874909 100-101 23.706325301882554 25.404823910655455 27.25225559697211 23.636595190489885 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1140.0 1 769.5 2 567.0 3 754.0 4 608.5 5 554.5 6 1073.0 7 2215.0 8 3506.5 9 4127.5 10 3370.5 11 2416.0 12 2261.0 13 2270.0 14 2361.5 15 2473.5 16 2673.0 17 2905.5 18 3143.5 19 3470.5 20 3936.0 21 4722.0 22 5791.0 23 7083.0 24 8769.0 25 11119.0 26 13949.0 27 16801.5 28 19225.0 29 21561.5 30 24561.0 31 28875.5 32 34903.0 33 37546.0 34 38084.0 35 40913.0 36 45044.5 37 49799.5 38 53338.5 39 55585.0 40 58251.5 41 62380.0 42 65453.5 43 67382.0 44 69789.0 45 73679.5 46 76864.5 47 79573.5 48 80917.0 49 81124.5 50 82121.0 51 81866.0 52 82780.5 53 85140.0 54 86560.0 55 81400.0 56 73426.5 57 67336.0 58 61229.0 59 53112.5 60 42731.5 61 35239.0 62 29087.5 63 21716.5 64 15654.5 65 11665.0 66 8684.0 67 6690.5 68 5166.0 69 3972.5 70 3079.0 71 2179.5 72 1540.0 73 1152.0 74 869.0 75 642.5 76 449.5 77 276.0 78 154.0 79 89.5 80 65.5 81 49.5 82 29.5 83 18.0 84 15.0 85 13.0 86 11.0 87 8.5 88 5.0 89 4.0 90 3.5 91 2.5 92 3.0 93 1.5 94 1.0 95 0.5 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4638808352461359 2 0.038330945700373334 3 0.05046383589861577 4 0.05401382969736078 5 0.0811106178067689 6 0.08164985737113523 7 0.04668915894805146 8 0.04152144645620746 9 0.04597017286222969 10-11 0.049632508236884344 12-13 0.045790426340774244 14-15 0.03821860412446368 16-17 0.038083794233372104 18-19 0.04572302139522845 20-21 0.05316003372044742 22-23 0.04543093329786335 24-25 0.06358533196486314 26-27 0.05531699197791275 28-29 0.04230783748757502 30-31 0.0440603660717656 32-33 0.043790746289582426 34-35 0.047475549979419024 36-37 0.042869545367123285 38-39 0.025411664470763328 40-41 0.02287274485520519 42-43 0.022917681485569052 44-45 0.0157952255728971 46-47 0.02801798903186726 48-49 0.027838242510411816 50-51 0.016536679973900806 52-53 0.016222123561353777 54-55 0.01575028894253324 56-57 0.015166112747803048 58-59 0.013278774272520891 60-61 0.015255986008530769 62-63 0.016334465137263433 64-65 0.01689617301681169 66-67 0.009077199333499899 68-69 0.011054411069509777 70-71 0.017120856168630995 72-73 0.015255986008530769 74-75 0.012694598077790699 76-77 0.008425618193223915 78-79 0.014087633619070387 80-81 0.01795218383036242 82-83 0.01572782062735131 84-85 0.01330124258770282 86-87 0.0032354373861979836 88-89 0.02779330588004796 90-91 0.030511972017061538 92-93 0.016019908724716404 94-95 0.03132083136361104 96-97 0.02606324561103931 98-99 0.023007554746296774 100-101 0.14456113988054045 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2225356.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.52288440875166 #Duplication Level Percentage of deduplicated Percentage of total 1 77.239665851742 42.88569038856874 2 13.2157301309974 14.6755091288125 3 4.224910375501418 7.037376312489024 4 1.772609264563441 3.9368151719295295 5 0.9161511545340746 2.5433677327069906 6 0.5464870600667695 1.820552272017946 7 0.3638896954025828 1.4142943846761422 8 0.2643814676208923 1.174337733322474 9 0.19526378054390742 0.9757447484719699 >10 1.117508749087858 11.67311940796494 >50 0.09484501315992157 3.6105690617660278 >100 0.043192712730048904 4.287484774104956 >500 0.003170076029321394 1.2299160559552968 >1k 0.001950816018043846 1.6699003370908707 >5k 2.4385200225548074E-4 1.0653224901226994 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9759 0.43853657572091836 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8588 0.3859157815648373 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5258 0.23627680245318053 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2842 0.12770990349409264 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2596 0.11665549242458285 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2385 0.1071738634178082 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2253 0.10124222820977857 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3480989109158266E-4 2 0.0 0.0 0.0 0.0 1.3480989109158266E-4 3 0.0 0.0 0.0 0.0 1.3480989109158266E-4 4 0.0 0.0 0.0 0.0 1.3480989109158266E-4 5 4.4936630363860886E-5 0.0 0.0 0.0 1.3480989109158266E-4 6 4.4936630363860886E-5 0.0 0.0 0.0 1.3480989109158266E-4 7 4.4936630363860886E-5 0.0 0.0 0.0 1.3480989109158266E-4 8 4.4936630363860886E-5 0.0 0.0 8.987326072772177E-5 1.3480989109158266E-4 9 4.4936630363860886E-5 0.0 0.0 1.3480989109158266E-4 1.3480989109158266E-4 10-11 4.4936630363860886E-5 0.0 0.0 1.3480989109158266E-4 2.0221483663737398E-4 12-13 8.987326072772177E-5 0.0 0.0 1.7974652145544355E-4 5.167712491844002E-4 14-15 1.3480989109158266E-4 0.0 0.0 2.246831518193044E-4 5.392395643663306E-4 16-17 2.0221483663737398E-4 0.0 0.0 2.4715146700123486E-4 5.617078795482611E-4 18-19 2.246831518193044E-4 0.0 0.0 3.3702472772895664E-4 7.63922716185635E-4 20-21 2.246831518193044E-4 0.0 0.0 4.0442967327474795E-4 7.863910313675654E-4 22-23 2.246831518193044E-4 0.0 0.0 4.0442967327474795E-4 8.088593465494959E-4 24-25 2.246831518193044E-4 0.0 0.0 5.167712491844002E-4 8.088593465494959E-4 26-27 2.246831518193044E-4 0.0 0.0 9.661375528230089E-4 8.088593465494959E-4 28-29 2.246831518193044E-4 0.0 0.0 0.0038870185264739663 8.088593465494959E-4 30-31 2.246831518193044E-4 0.0 0.0 0.01211042188306051 8.088593465494959E-4 32-33 2.246831518193044E-4 0.0 0.0 0.03053444033224347 8.088593465494959E-4 34-35 2.246831518193044E-4 0.0 0.0 0.05666509088882857 8.088593465494959E-4 36-37 2.246831518193044E-4 0.0 0.0 0.10157925293750752 8.088593465494959E-4 38-39 2.246831518193044E-4 0.0 0.0 0.16920888163511816 8.537959769133568E-4 40-41 2.246831518193044E-4 0.0 0.0 0.23888312701428444 8.537959769133568E-4 42-43 2.246831518193044E-4 0.0 0.0 0.30581623794125523 8.537959769133568E-4 44-45 2.246831518193044E-4 0.0 0.0 0.38888159916885207 9.212009224591482E-4 46-47 2.246831518193044E-4 0.0 0.0 0.48879819678289677 0.0010560108135507307 48-49 2.246831518193044E-4 0.0 0.0 0.5950283909630638 0.0011683523894603829 50-51 2.246831518193044E-4 0.0 0.0 0.7075272450789896 0.0012582256501881047 52-53 2.246831518193044E-4 0.0 0.0 0.8118251641535107 0.0012582256501881047 54-55 2.246831518193044E-4 0.0 0.0 0.9272448992430874 0.0012582256501881047 56-57 2.246831518193044E-4 0.0 0.0 1.056887077842826 0.001280693965370035 58-59 2.246831518193044E-4 0.0 0.0 1.1937865222463282 0.0013031622805519656 60-61 2.246831518193044E-4 4.4936630363860886E-5 0.0 1.3429986033695283 0.0013031622805519656 62-63 2.246831518193044E-4 4.4936630363860886E-5 0.0 1.4874024650437954 0.00152784543237127 64-65 2.246831518193044E-4 4.4936630363860886E-5 0.0 1.6352215106257155 0.001572782062735131 66-67 2.246831518193044E-4 4.4936630363860886E-5 0.0 1.8058009594869315 0.0016177186930989918 68-69 3.594930429108871E-4 4.4936630363860886E-5 0.0 1.99619746233861 0.0016626553234628527 70-71 3.594930429108871E-4 4.4936630363860886E-5 0.0 2.191649336106223 0.001685123638644783 72-73 3.594930429108871E-4 4.4936630363860886E-5 0.0 2.386022730745103 0.0017525285841905745 74-75 3.594930429108871E-4 4.4936630363860886E-5 0.0 2.580261315492892 0.0018424018449182962 76-77 3.594930429108871E-4 4.4936630363860886E-5 0.0 2.7820717224569913 0.0019996800511918092 78-79 4.0442967327474795E-4 4.4936630363860886E-5 0.0 3.014146949971151 0.0020221483663737396 80-81 4.0442967327474795E-4 4.4936630363860886E-5 0.0 3.2638822732183073 0.0020670849967376007 82-83 4.0442967327474795E-4 4.4936630363860886E-5 0.0 3.516987843742754 0.0022468315181930444 84-85 4.0442967327474795E-4 4.4936630363860886E-5 0.0 3.7675320263364602 0.0022468315181930444 86-87 4.0442967327474795E-4 8.987326072772177E-5 0.0 4.026479358808208 0.0022468315181930444 88-89 4.0442967327474795E-4 8.987326072772177E-5 0.0 4.300660208973306 0.0022468315181930444 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3945 0.0 59.927513 1 GTATCAA 7010 0.0 44.08129 1 TATCAAC 8905 0.0 34.375072 2 ATCAACG 8995 0.0 33.92544 3 TCAACGC 9060 0.0 33.786976 4 CAACGCA 9185 0.0 33.171917 5 AACGCAG 9470 0.0 32.217274 6 CGCGTAA 45 2.4135763E-5 31.668459 10-11 TGGTATC 1525 0.0 30.233856 2 GTGGTAT 1765 0.0 27.871513 1 ACGCAGA 11105 0.0 27.335058 7 CGCAGAG 11030 0.0 27.30558 8 GCAGAGT 12200 0.0 23.946026 9 TACATGG 7135 0.0 23.649723 2 GTACATG 7440 0.0 23.045683 1 GAGTACT 8200 0.0 22.216198 12-13 CAGAGTA 11865 0.0 21.679483 10-11 ACATGGG 7295 0.0 21.56723 3 GTATAGG 570 0.0 20.947548 1 TAAGGTG 1120 0.0 20.795517 5 >>END_MODULE