FastQCFastQC Report
Thu 26 May 2016
SRR938166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225356
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95290.42820115073723036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86700.38960058525467384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53780.24166919809684384No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27520.12366560676134515No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25330.11382448471165961No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23030.10348905972797162No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22620.10164665788305331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40000.059.6185871
GTATCAA68950.041.6141171
TGGTATC14900.033.79542
ATCAACG88050.032.2079733
TCAACGC89800.031.6832644
TATCAAC90000.031.5115532
CAACGCA91800.030.9929985
AACGCAG94850.029.9957126
GTGGTAT18300.028.295241
ACGCAGA113150.025.1018967
CGCAGAG113500.024.9826438
GTAAGGT12150.022.6741584
TAAGGTG12600.022.618315
GCAGAGT125700.022.4067739
GTACATG71200.021.8842581
TACATGG68300.021.7701592
GAGTACT84800.021.64778912-13
CAGAGTA119450.021.19341510-11
GTACTTT88200.020.62481714-15
AGAGTAC117250.020.13276710-11
Produced by FastQC (version 0.11.5)