FastQCFastQC Report
Thu 26 May 2016
SRR938165_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938165_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241932
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100610.4487647261379917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87090.38845959645520023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54160.24157735381804624No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28710.12805919180421174No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26480.11811241375741995No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA24110.10754117430858742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39150.058.2986221
GTATCAA70350.042.692211
TGGTATC15300.033.8530242
TATCAAC89900.033.0884972
ATCAACG90450.032.834763
TCAACGC91900.032.420114
CAACGCA93000.032.0877385
AACGCAG96550.031.0555696
GTGGTAT19650.028.673561
ACGCAGA112450.026.382187
CGCAGAG111850.026.1836538
TAAGGTA8900.026.1618524
TAGCGCG1551.4068646E-624.525664
TACATGG70700.024.4649522
GTACATG73050.024.0541361
GCAGAGT123250.023.3760559
ACATGGG72500.022.6122363
GAGTACT85400.021.6820912-13
CAGAGTA119500.021.0852610-11
GTACTTT88150.020.9379514-15
Produced by FastQC (version 0.11.5)