##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938165_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2241932 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.900436320102482 33.0 31.0 34.0 30.0 34.0 2 32.091599120758346 34.0 31.0 34.0 30.0 34.0 3 32.16346659934378 34.0 31.0 34.0 30.0 34.0 4 35.67571853205182 37.0 35.0 37.0 35.0 37.0 5 35.699083201453035 37.0 35.0 37.0 35.0 37.0 6 35.72093667426131 37.0 36.0 37.0 35.0 37.0 7 35.65946067944969 37.0 35.0 37.0 33.0 37.0 8 35.638541668525185 37.0 35.0 37.0 33.0 37.0 9 37.30846118437134 39.0 37.0 39.0 34.0 39.0 10-11 37.23257128226904 39.0 37.0 39.0 34.0 39.0 12-13 37.135753671387 39.0 37.0 39.0 34.0 39.0 14-15 38.39779774765693 40.0 38.0 41.0 34.0 41.0 16-17 38.31572634674022 40.0 38.0 41.0 33.0 41.0 18-19 38.25707514768512 40.0 38.0 41.0 33.5 41.0 20-21 38.15143345114838 40.0 38.0 41.0 33.0 41.0 22-23 38.00418701370069 40.0 38.0 41.0 33.0 41.0 24-25 37.95863010118059 40.0 38.0 41.0 33.0 41.0 26-27 37.964301771864626 40.0 38.0 41.0 33.0 41.0 28-29 37.87585997255938 40.0 38.0 41.0 33.0 41.0 30-31 37.68912750252907 40.0 37.5 41.0 33.0 41.0 32-33 37.468142432509104 40.0 37.0 41.0 32.0 41.0 34-35 37.2681803016327 40.0 37.0 41.0 31.0 41.0 36-37 37.05067571184139 40.0 36.5 41.0 31.0 41.0 38-39 36.81648194503669 40.0 36.0 41.0 30.0 41.0 40-41 36.595692465248725 39.0 36.0 41.0 30.0 41.0 42-43 36.315687317902594 39.0 35.0 41.0 30.0 41.0 44-45 36.1056900922954 39.0 35.0 41.0 29.0 41.0 46-47 35.971327631703375 39.0 35.0 41.0 28.5 41.0 48-49 35.724199039043114 39.0 35.0 41.0 27.0 41.0 50-51 35.07468201533321 38.5 34.5 40.5 26.5 40.5 52-53 35.15266609335163 38.5 35.0 40.0 26.5 41.0 54-55 35.29852801066223 39.0 35.0 40.5 26.5 41.0 56-57 35.093638433279864 39.0 35.0 40.5 26.0 41.0 58-59 34.832056012403584 38.0 34.5 40.0 26.0 41.0 60-61 34.45446895802371 38.0 34.0 40.0 24.5 41.0 62-63 34.03911648524576 37.5 34.0 40.0 23.0 41.0 64-65 33.61472292647591 37.0 33.5 40.0 22.0 41.0 66-67 33.17754485863086 36.0 33.0 39.0 21.5 41.0 68-69 32.69520886449723 36.0 32.5 39.0 19.5 40.5 70-71 32.20363664018356 35.0 32.0 38.5 18.0 40.0 72-73 31.71738259679598 35.0 31.5 37.5 14.0 40.0 74-75 31.199044841681193 35.0 31.0 37.0 9.0 39.0 76-77 30.666174085565487 35.0 30.5 36.5 7.0 39.0 78-79 30.103541499028516 34.0 30.0 36.0 2.0 38.5 80-81 29.593419648767224 34.0 29.5 35.0 2.0 37.0 82-83 29.06890463225468 34.0 29.0 35.0 2.0 37.0 84-85 28.59259201438759 34.0 29.0 35.0 2.0 36.0 86-87 28.10515595477472 34.0 27.5 35.0 2.0 36.0 88-89 27.57120889482821 33.5 26.5 35.0 2.0 35.5 90-91 26.982227382454063 33.0 25.5 35.0 2.0 35.0 92-93 26.385926736404137 33.0 24.5 35.0 2.0 35.0 94-95 25.806654037678214 32.0 23.5 35.0 2.0 35.0 96-97 24.99994915100012 32.0 19.0 34.5 2.0 35.0 98-99 23.95948672841103 31.5 2.0 34.0 2.0 35.0 100-101 21.096508725509963 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28051219132120053 1101 2 0.1726697954700711 1101 3 0.1712895837146604 1101 4 0.1138290515153102 1101 5 0.1466500601348173 1101 6 0.12405197869190232 1101 7 0.055240937289354974 1101 8 0.056628199173559324 1101 9 0.021437092875963515 1101 10-11 0.1350830688242297 1101 12-13 0.055273507655805076 1101 14-15 0.09071678322654009 1101 16-17 0.1261910293925652 1101 18-19 0.12455890862077013 1101 20-21 0.2590585639734826 1101 22-23 0.3231024887106315 1101 24-25 0.2107086097095916 1101 26-27 0.18350109740087817 1101 28-29 0.16736274625353076 1101 30-31 0.3126481267592567 1101 32-33 0.09764237536345632 1101 34-35 0.2115790945491085 1101 36-37 0.17396485095358827 1101 38-39 0.3352651568422047 1101 40-41 0.19107463540489533 1101 42-43 0.22495262260563464 1101 44-45 0.08246889662968471 1101 46-47 0.2765746184157081 1101 48-49 0.38453860480336033 1101 50-51 0.36944667153508703 1101 52-53 0.42842442377325796 1101 54-55 0.41645123544259377 1101 56-57 0.49992770511031637 1101 58-59 0.31637886052944 1101 60-61 0.34048854789288896 1101 62-63 0.38411306550214874 1101 64-65 0.26712453769024336 1101 66-67 0.1313140575622569 1101 68-69 0.21466713923853575 1101 70-71 0.33370008777642823 1101 72-73 0.5774866188448975 1101 74-75 0.4045617949505633 1101 76-77 0.38387589858015403 1101 78-79 0.4150861292772312 1101 80-81 0.507723351263742 1101 82-83 0.2752861128726174 1101 84-85 0.30150465302347484 1101 86-87 0.32784066847441906 1101 88-89 0.1970887741442553 1101 90-91 0.5982211470303263 1101 92-93 0.6204608601424795 1101 94-95 0.5826622153076961 1101 96-97 0.608322846710891 1101 98-99 0.755149149062472 1101 100-101 0.5507043769175262 1102 1 0.49611529209639116 1102 2 0.4054305189842822 1102 3 0.2917071547740946 1102 4 0.2884564416961908 1102 5 0.22028313507021835 1102 6 0.2871403507849237 1102 7 0.2181639347053732 1102 8 0.16021476248105415 1102 9 0.3588551807312612 1102 10-11 0.41664999388882507 1102 12-13 0.14756704770748996 1102 14-15 0.33310231294230874 1102 16-17 0.3666974893408863 1102 18-19 0.3764142057784028 1102 20-21 0.44029680686753636 1102 22-23 0.4651407316046843 1102 24-25 0.3511840619059754 1102 26-27 0.4301470922329216 1102 28-29 0.4403239865636053 1102 30-31 0.3008610466558963 1102 32-33 0.4709839774306346 1102 34-35 0.3868576475206851 1102 36-37 0.3304320344161269 1102 38-39 0.37822122919362755 1102 40-41 0.27320590155476054 1102 42-43 0.5327210980578343 1102 44-45 0.4715603694979009 1102 46-47 0.6127937398627381 1102 48-49 0.3918233593253291 1102 50-51 0.5765577826461978 1102 52-53 0.5042280413443123 1102 54-55 0.4048750080524073 1102 56-57 0.43800935885709436 1102 58-59 0.448184545283965 1102 60-61 0.4179107701151139 1102 62-63 0.41606345309904924 1102 64-65 0.38950179867214985 1102 66-67 0.3251269774589005 1102 68-69 0.17388987825662383 1102 70-71 0.2552608112906469 1102 72-73 0.3204013475270635 1102 74-75 0.2930827251831225 1102 76-77 0.262280032947082 1102 78-79 0.3518737261764535 1102 80-81 0.37015642619914146 1102 82-83 0.46329644878992937 1102 84-85 0.3774653765376854 1102 86-87 0.526135242117828 1102 88-89 0.562926500242444 1102 90-91 0.5239017671853681 1102 92-93 0.6981497490313693 1102 94-95 0.7135831455402517 1102 96-97 0.6280437769434499 1102 98-99 0.8473682705095023 1102 100-101 0.6616459789847084 1103 1 0.46715979171297306 1103 2 0.36599712162090015 1103 3 0.2910605272340874 1103 4 0.2519939878522308 1103 5 0.2776993582086007 1103 6 0.3370146949831181 1103 7 0.3697589914845878 1103 8 0.2738374711291556 1103 9 0.3691678143908206 1103 10-11 0.36770585694045366 1103 12-13 0.3775367789584507 1103 14-15 0.4670853108138786 1103 16-17 0.4006846630850234 1103 18-19 0.35607441629299075 1103 20-21 0.4367212476169726 1103 22-23 0.44773885828294624 1103 24-25 0.5253465463729938 1103 26-27 0.41391180585664245 1103 28-29 0.5698975160870248 1103 30-31 0.5587145975395345 1103 32-33 0.5606481866595701 1103 34-35 0.5700829143336321 1103 36-37 0.6286586805324319 1103 38-39 0.7168603249780148 1103 40-41 0.7796137147388791 1103 42-43 0.817487261848207 1103 44-45 0.7403571107988043 1103 46-47 0.8474897996106137 1103 48-49 0.8482998845944181 1103 50-51 0.706076772743053 1103 52-53 0.7474299738875274 1103 54-55 0.8571564264318212 1103 56-57 0.7752206858481614 1103 58-59 0.8903818641206911 1103 60-61 0.8296748358458714 1103 62-63 0.9132887755903027 1103 64-65 0.8532459872495011 1103 66-67 0.9286873517183167 1103 68-69 0.9628717752163354 1103 70-71 1.0985404403719272 1103 72-73 1.0954402590669012 1103 74-75 0.8110105053651857 1103 76-77 0.9392130190502819 1103 78-79 0.9721847907202594 1103 80-81 0.9384933153512378 1103 82-83 0.9622890511684048 1103 84-85 0.9097427202778015 1103 86-87 1.0446738385690963 1103 88-89 1.0880097013398142 1103 90-91 0.9829915028905951 1103 92-93 1.1826203116605996 1103 94-95 0.9914848075375353 1103 96-97 1.053252001134659 1103 98-99 1.298469815076185 1103 100-101 1.1137979453145235 1104 1 0.33346923699661346 1104 2 0.3200680461989336 1104 3 0.21160756233468447 1104 4 0.2344836676474742 1104 5 0.1990621106693169 1104 6 0.20916218277353238 1104 7 0.14784579637584727 1104 8 0.19277591540290473 1104 9 0.13645516867771335 1104 10-11 0.2392315625074133 1104 12-13 0.2215117972573708 1104 14-15 0.24355876573383028 1104 16-17 0.39527946476672327 1104 18-19 0.2282090543933748 1104 20-21 0.17051279137872655 1104 22-23 0.2672226053287048 1104 24-25 0.47644038813525214 1104 26-27 0.4246420109091389 1104 28-29 0.3526513759911367 1104 30-31 0.38528447272621236 1104 32-33 0.40302080101943005 1104 34-35 0.5038410964927422 1104 36-37 0.6314746663083071 1104 38-39 0.5829891607294684 1104 40-41 0.6365766762212175 1104 42-43 0.5188775983102047 1104 44-45 0.5421606361826505 1104 46-47 0.4604576116129877 1104 48-49 0.6760317049005451 1104 50-51 0.7311415209033996 1104 52-53 0.6787762216352604 1104 54-55 0.6511839856855417 1104 56-57 0.6747082687643555 1104 58-59 0.6849693367199166 1104 60-61 0.6808197432281631 1104 62-63 0.768740470749961 1104 64-65 0.8284170546970415 1104 66-67 0.7996864579509833 1104 68-69 0.9558686941462184 1104 70-71 0.971647609642325 1104 72-73 1.0500677655893078 1104 74-75 0.9484774412090609 1104 76-77 1.0528854223896822 1104 78-79 0.9579973051761606 1104 80-81 0.9369820490285612 1104 82-83 0.9592705637958439 1104 84-85 0.8071174810118151 1104 86-87 0.8390457219243679 1104 88-89 0.9617305622880821 1104 90-91 0.9109449565541361 1104 92-93 1.0330703839520048 1104 94-95 1.2386571618577413 1104 96-97 1.1213879584698816 1104 98-99 1.1622818993054302 1104 100-101 1.0245865926609667 1105 1 0.3289088976341006 1105 2 0.28608022547921763 1105 3 0.30175228575797064 1105 4 0.23034433056380266 1105 5 0.18368330197684202 1105 6 0.24136774569403485 1105 7 0.23788515808837474 1105 8 0.23110090426657592 1105 9 0.326942917822592 1105 10-11 0.31162128169400205 1105 12-13 0.238975611955901 1105 14-15 0.3741490491612822 1105 16-17 0.3455085042324626 1105 18-19 0.3737678137747906 1105 20-21 0.3567976307621379 1105 22-23 0.33450828316015446 1105 24-25 0.3046970208105435 1105 26-27 0.3138408351258022 1105 28-29 0.4647589036185735 1105 30-31 0.2836584347799871 1105 32-33 0.30581084135918246 1105 34-35 0.4159516471566107 1105 36-37 0.3782733596452701 1105 38-39 0.48375799002305087 1105 40-41 0.5756649373262093 1105 42-43 0.4687460962256438 1105 44-45 0.5438817664070612 1105 46-47 0.515097216768865 1105 48-49 0.3550468085240155 1105 50-51 0.2748092820870838 1105 52-53 0.3141706878781676 1105 54-55 0.3445665144910848 1105 56-57 0.5987838197794275 1105 58-59 0.4969002389984851 1105 60-61 0.5370883649102751 1105 62-63 0.6260867160053465 1105 64-65 0.5582407316518143 1105 66-67 0.7114620295049221 1105 68-69 0.6992802529927289 1105 70-71 0.7232847172366377 1105 72-73 0.581309735648798 1105 74-75 0.6162083945235999 1105 76-77 0.5194693112059561 1105 78-79 0.5017911003049846 1105 80-81 0.6292565016144565 1105 82-83 0.7342888576118654 1105 84-85 0.7078558576590339 1105 86-87 0.8943335016552645 1105 88-89 0.9688687070506248 1105 90-91 0.8139859533737273 1105 92-93 0.9404136506273808 1105 94-95 0.9570046367776541 1105 96-97 1.082078259946634 1105 98-99 0.9020962670862609 1105 100-101 0.7826576553560756 1106 1 -0.12187388926488296 1106 2 0.0034705280707996167 1106 3 -0.05952946390438285 1106 4 0.005452494738754865 1106 5 -0.05404957356481077 1106 6 -0.02239197735205778 1106 7 -0.06347774402932771 1106 8 -0.11836447481911705 1106 9 -3.094272705581602E-4 1106 10-11 -0.5733945868534249 1106 12-13 -0.34445030186800096 1106 14-15 -0.2036594072496527 1106 16-17 -0.12045182775029417 1106 18-19 -0.00979273973086947 1106 20-21 -0.06235425653933646 1106 22-23 -0.21667699846885569 1106 24-25 -0.1279598884588964 1106 26-27 -0.11033406743428031 1106 28-29 -0.13838267499188817 1106 30-31 -0.09820864979752741 1106 32-33 -0.21634919972812128 1106 34-35 -0.34461614354613346 1106 36-37 -0.3301058450171013 1106 38-39 -0.21786304828600578 1106 40-41 -0.10478910085884507 1106 42-43 -0.283030527577516 1106 44-45 -0.0434153524545664 1106 46-47 0.14491601035709323 1106 48-49 -0.06724967358491796 1106 50-51 0.01256205615047179 1106 52-53 -0.03473857988974771 1106 54-55 -0.2972209257295688 1106 56-57 -0.26708548170287116 1106 58-59 -0.02725593800535364 1106 60-61 -0.28407335686901547 1106 62-63 -0.19560598211690206 1106 64-65 -0.08304945498375105 1106 66-67 -0.42893356148535844 1106 68-69 -0.6363885384171368 1106 70-71 -0.2705544909781423 1106 72-73 -0.1495939672356883 1106 74-75 -0.1515356409468751 1106 76-77 -0.08218783768358051 1106 78-79 0.12122056151166305 1106 80-81 -0.03714331540292548 1106 82-83 -0.09411864903214351 1106 84-85 -0.23067593305711043 1106 86-87 -0.16878021064646376 1106 88-89 -0.2613295408740619 1106 90-91 -0.2758682302590465 1106 92-93 -0.06491496403334551 1106 94-95 -0.15850774806226653 1106 96-97 -0.05329620090815723 1106 98-99 -0.11808271906939538 1106 100-101 -0.29679525678210794 1107 1 0.28735170605256855 1107 2 0.2814850467698946 1107 3 0.25339865361645764 1107 4 0.18961206711323086 1107 5 0.22992446799155175 1107 6 0.2522539428744537 1107 7 0.1727947212292733 1107 8 0.27103836670677595 1107 9 0.2120670177748707 1107 10-11 0.2708301514354332 1107 12-13 0.21074075202322717 1107 14-15 0.2530285105517862 1107 16-17 0.37089813511180836 1107 18-19 0.32002915703209567 1107 20-21 0.3735181826909866 1107 22-23 0.4738531530664929 1107 24-25 0.4692481822110395 1107 26-27 0.5230164064707878 1107 28-29 0.5214892627179992 1107 30-31 0.5344645334605787 1107 32-33 0.4638478057505111 1107 34-35 0.4687607236652269 1107 36-37 0.4970390277300183 1107 38-39 0.5016354630236037 1107 40-41 0.5666999386977878 1107 42-43 0.5184380067766341 1107 44-45 0.5254984749081402 1107 46-47 0.4979919494382372 1107 48-49 0.4096510253695058 1107 50-51 0.4310590343311631 1107 52-53 0.40692991193039063 1107 54-55 0.44399889575455376 1107 56-57 0.5903660644401896 1107 58-59 0.4785651112515694 1107 60-61 0.32952736938335647 1107 62-63 0.48253732893368806 1107 64-65 0.5033203782448368 1107 66-67 0.5026501638591938 1107 68-69 0.5125987394696168 1107 70-71 0.6098681986949757 1107 72-73 0.562208744783657 1107 74-75 0.4731977279372721 1107 76-77 0.6399944427742632 1107 78-79 0.58893558046152 1107 80-81 0.7652830277513196 1107 82-83 0.5812393191117842 1107 84-85 0.5871709730696892 1107 86-87 0.8270723264755695 1107 88-89 0.7363866366514742 1107 90-91 0.6158636658056018 1107 92-93 0.6630736850991532 1107 94-95 0.6515548785081542 1107 96-97 0.6278439327883021 1107 98-99 0.7135456828579656 1107 100-101 0.73414585583145 1108 1 0.1660449528992416 1108 2 0.19732777145635083 1108 3 0.11601982956943857 1108 4 0.07380709611221903 1108 5 0.10059059758479094 1108 6 0.06629920567875303 1108 7 0.028575642835377835 1108 8 0.12431030323302394 1108 9 0.05056893964555087 1108 10-11 0.13059639072245943 1108 12-13 0.07742342189251161 1108 14-15 0.13579477064792655 1108 16-17 0.1315237909706113 1108 18-19 0.17021985773455128 1108 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-0.9730315532496974 2316 66-67 -1.1530880688940286 2316 68-69 -1.1726933855889712 2316 70-71 -1.0715717140559384 2316 72-73 -0.9284466466228132 2316 74-75 -0.9685366328291032 2316 76-77 -1.0670387943278854 2316 78-79 -0.7927679143829884 2316 80-81 -0.8844032407507356 2316 82-83 -1.0513986512943347 2316 84-85 -1.2080143606627587 2316 86-87 -1.2448750876617645 2316 88-89 -1.2576306467357377 2316 90-91 -1.163990005554222 2316 92-93 -1.3697686614048585 2316 94-95 -1.5480249015342942 2316 96-97 -1.3913518507215272 2316 98-99 -1.3607202774729892 2316 100-101 -1.2185050790263716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5898.0 3 1710.0 4 1702.0 5 1828.0 6 2303.0 7 2608.0 8 3355.0 9 4194.0 10 5223.0 11 6495.0 12 8190.0 13 9861.0 14 11507.0 15 14135.0 16 17150.0 17 22259.0 18 20281.0 19 16827.0 20 16258.0 21 16763.0 22 18109.0 23 19455.0 24 21732.0 25 24405.0 26 27729.0 27 32119.0 28 37187.0 29 44007.0 30 52777.0 31 64067.0 32 80607.0 33 104805.0 34 144424.0 35 213525.0 36 324001.0 37 453153.0 38 348600.0 39 42676.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.90435575113093 18.17048859026585 14.141991488705477 23.783164169897738 2 15.783521910302397 19.307993777272404 40.10330156219737 24.805182750227818 3 19.32979982540734 23.076401248926647 29.63380171057964 27.95999721508638 4 12.868981166974494 16.054178982032884 36.080057988971745 34.99678186202088 5 13.725495447839315 36.99336426883082 34.4254947782398 14.855645505090074 6 33.559377887046786 35.25861911142923 16.635283579716987 14.546719421806998 7 29.18311124045575 32.53963436135539 19.913906462996472 18.363347935192383 8 26.899777302729937 34.86622543747294 19.176287873040245 19.057709386756876 9 26.549728219637803 16.394023509670742 19.849739822740293 37.20650844795116 10-11 25.434150687695407 26.165881630328236 26.657905684936768 21.742061997039595 12-13 26.129068556116636 23.481562814938716 27.329681365183568 23.059687263761077 14-15 22.859679733257583 25.02060215645946 25.51286705924384 26.606851051039122 16-17 22.372246452232563 28.189286268992987 26.58406544066583 22.85440183810862 18-19 22.459820850758057 26.515158953668454 28.959046501144787 22.0659736944287 20-21 22.785969294608034 25.617495841996767 29.54684237261958 22.049692490775616 22-23 22.55598609143225 25.58632834476573 29.37945033886568 22.478235224936334 24-25 22.401461093932028 25.885309394274152 29.405252000533043 22.307977511260777 26-27 21.99151594843689 26.25858146550743 29.149287260219985 22.600615325835697 28-29 22.161867095034328 26.68693200407241 29.04084147644015 22.11035942445311 30-31 22.201206109149734 26.206795428113843 29.05175967356806 22.540238789168363 32-33 21.97335628222585 26.08179426297029 29.105731918875726 22.839117535928132 34-35 22.682404688286027 26.274323117322762 28.84667146065249 22.196600733738727 36-37 22.649056489239243 25.958920893461862 28.87776294417671 22.514259673122183 38-39 22.31079528340826 26.232572148905074 28.86801798284267 22.588614584843988 40-41 22.638199660989493 26.060411877460886 28.924686966391462 22.376701495158155 42-43 22.30691225835097 26.064996045261395 28.735119529045853 22.892972167341778 44-45 22.393550574630595 26.416232422014417 28.899970554643446 22.290246448711542 46-47 22.76997457480222 26.646597781937604 28.208941272634103 22.374486370626077 48-49 22.552423410856274 26.627570195514334 28.180691194615964 22.639315199013428 50-51 22.705302370695474 26.897715279632134 28.072700941153006 22.32428140851939 52-53 22.850721330272673 26.605118801433957 28.020206617008984 22.523953251284386 54-55 22.4241554911701 26.797300034532572 28.17739838544599 22.60114608885133 56-57 22.583929455377902 26.993236030867845 27.92572728674067 22.49710722701358 58-59 22.53352190216579 26.62061750344086 28.00771377392709 22.838146820466264 60-61 22.398022729508675 26.796932009352727 28.216260279042498 22.5887849820961 62-63 22.334442799441526 26.8440979652402 28.45130324160255 22.370155993715727 64-65 22.194442232306596 26.802500109863313 28.249773636953158 22.75328402087693 66-67 22.15324781001904 26.927642743574598 28.10188564096982 22.817223805436544 68-69 22.50336771574628 26.591827051875207 28.26735346728057 22.637451765097953 70-71 22.317160103926934 26.786999985497527 28.024886239275144 22.87095367130039 72-73 22.29211896834723 26.848307972460645 28.043600338853953 22.815972720338177 74-75 22.40198802571012 27.243506155245463 27.62499506406894 22.729510754975475 76-77 22.57564330183774 26.90672828819351 27.783536764231854 22.734091645736896 78-79 22.54253038266861 26.944281116750997 27.841173211323827 22.672015289256567 80-81 22.701933923301993 27.051147842446117 27.588905174877087 22.658013059374806 82-83 22.62177095307173 27.144126420994795 27.597051259429524 22.63705136650395 84-85 22.54239549018698 27.275450677935254 27.563894371299373 22.618259460578397 86-87 22.81763631736521 27.17844928897437 27.408829756886043 22.59508463677438 88-89 22.62499550979801 27.210668675302287 27.310802968598036 22.85353284630167 90-91 22.713701790158748 27.172474764041166 27.32126416545167 22.792559280348417 92-93 22.829766856203044 26.80516721061606 27.496033880510208 22.86903205267069 94-95 22.664931808794623 26.800028472971764 27.627886821276093 22.90715289695752 96-97 22.991360387548713 26.426611487859873 27.446131273380075 23.135896851211335 98-99 22.62205836027596 26.704837001189553 27.246136190635124 23.42696844789937 100-101 23.17059031807322 26.050215738464445 27.559435583945884 23.21975835951645 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1057.0 1 724.5 2 593.5 3 793.5 4 633.5 5 588.0 6 1147.5 7 2341.0 8 3851.0 9 4452.5 10 3505.5 11 2493.0 12 2295.0 13 2411.0 14 2530.5 15 2607.0 16 2723.5 17 2924.5 18 3168.5 19 3552.0 20 3998.0 21 4690.0 22 5714.0 23 7007.5 24 8822.5 25 11151.5 26 13996.5 27 16927.5 28 19196.5 29 21675.5 30 24936.0 31 29025.0 32 35142.5 33 38070.5 34 38589.5 35 41282.0 36 45449.5 37 50385.0 38 53717.5 39 55925.5 40 58814.5 41 63365.5 42 66073.0 43 67538.5 44 70521.5 45 74810.5 46 78033.0 47 80782.5 48 81835.0 49 81815.0 50 83348.5 51 83376.5 52 84052.0 53 86154.5 54 87601.5 55 82183.5 56 73489.0 57 67045.0 58 61283.0 59 53295.0 60 42419.5 61 34761.5 62 28752.0 63 21232.0 64 15074.0 65 11238.0 66 8334.0 67 6357.5 68 4969.5 69 3862.0 70 2933.0 71 2024.0 72 1379.5 73 1053.0 74 799.0 75 584.5 76 425.0 77 276.0 78 171.5 79 99.5 80 53.0 81 35.5 82 28.5 83 17.0 84 10.0 85 9.5 86 8.0 87 8.0 88 8.5 89 7.0 90 4.0 91 2.0 92 2.5 93 2.5 94 1.5 95 0.5 96 0.5 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5017547365397345 2 0.049689285848098876 3 0.05745044898774807 4 0.06717420510523958 5 0.07970803753191444 6 0.10718433922170699 7 0.0703857208871634 8 0.05450655952098458 9 0.05138425251078088 10-11 0.07564903841864963 12-13 0.10745196553686731 14-15 0.05658066346347704 16-17 0.06101879985655229 18-19 0.05985908582419092 20-21 0.07466774192972847 22-23 0.06434182660312623 24-25 0.08771452479379392 26-27 0.0665943480890589 28-29 0.06641593054561869 30-31 0.0708094625528339 32-33 0.0709878800962741 34-35 0.07248212702258588 36-37 0.06793247966486049 38-39 0.04045617797506793 40-41 0.04453747928126277 42-43 0.04235186437412018 44-45 0.021945357843146 46-47 0.02905975738782443 48-49 0.028479900371643746 50-51 0.02611586792106094 52-53 0.02618277449985102 54-55 0.025424499940230126 56-57 0.02544680213316015 58-59 0.021722335913845738 60-61 0.029461196860564906 62-63 0.020674132846134497 64-65 0.022770538981556978 66-67 0.016057578909619025 68-69 0.019068374955172593 70-71 0.041682798586219386 72-73 0.03784682140225484 74-75 0.03182522931114771 76-77 0.022636725823976817 78-79 0.015968370137898917 80-81 0.017864056536951167 82-83 0.02232449512295645 84-85 0.018733842061222195 86-87 0.00916620129424086 88-89 0.041995029287239756 90-91 0.052677779700722405 92-93 0.033966239832430245 94-95 0.05399360908359398 96-97 0.043489276213551524 98-99 0.03345328939503964 100-101 0.164567881630665 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2241932.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.178810990448525 #Duplication Level Percentage of deduplicated Percentage of total 1 77.17396703963631 42.583677406631956 2 13.17228161582345 14.53661675184964 3 4.178141775056593 6.916346858914345 4 1.8467320828149862 4.076019221905818 5 0.955200554376964 2.6353415423969073 6 0.5709804522255835 1.8903613471557774 7 0.37319853565129646 1.4414856022430538 8 0.26461561858027577 1.1680940162209326 9 0.1848372340781213 0.917918892287455 >10 1.1355061666022872 11.881338339032899 >50 0.095761218810728 3.644077696425559 >100 0.04328099217379282 4.29657008997281 >500 0.0032333612762466793 1.220434336680167 >1k 0.002020850797654064 1.7129435079988007 >5k 1.6166806381232512E-4 0.6300210147506266 >10k+ 8.083403190616256E-5 0.4487533755331719 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10061 0.4487647261379917 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8709 0.38845959645520023 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5416 0.24157735381804624 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2871 0.12805919180421174 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2648 0.11811241375741995 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2411 0.10754117430858742 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.92087717201057E-5 2 0.0 0.0 0.0 0.0 8.92087717201057E-5 3 0.0 0.0 0.0 0.0 8.92087717201057E-5 4 0.0 0.0 0.0 0.0 8.92087717201057E-5 5 4.460438586005285E-5 0.0 0.0 0.0 8.92087717201057E-5 6 4.460438586005285E-5 0.0 0.0 0.0 1.3381315758015854E-4 7 4.460438586005285E-5 0.0 0.0 0.0 1.3381315758015854E-4 8 4.460438586005285E-5 0.0 0.0 0.0 1.3381315758015854E-4 9 4.460438586005285E-5 0.0 0.0 0.0 1.3381315758015854E-4 10-11 8.92087717201057E-5 0.0 0.0 2.2302192930026424E-5 1.3381315758015854E-4 12-13 8.92087717201057E-5 0.0 0.0 1.1151096465013212E-4 2.899285080903435E-4 14-15 8.92087717201057E-5 0.0 0.0 1.3381315758015854E-4 3.791372798104492E-4 16-17 8.92087717201057E-5 0.0 0.0 1.784175434402114E-4 4.906482444605813E-4 18-19 8.92087717201057E-5 0.0 0.0 1.784175434402114E-4 5.575548232506606E-4 20-21 1.3381315758015854E-4 0.0 0.0 2.2302192930026424E-4 5.79857016180687E-4 22-23 1.3381315758015854E-4 0.0 0.0 4.2374166567050206E-4 5.79857016180687E-4 24-25 2.0071973637023782E-4 0.0 0.0 6.467635949707663E-4 5.79857016180687E-4 26-27 2.2302192930026424E-4 0.0 0.0 9.812964889211627E-4 6.244614020407399E-4 28-29 2.2302192930026424E-4 0.0 0.0 0.0034122355182940427 6.244614020407399E-4 30-31 2.2302192930026424E-4 0.0 0.0 0.012667645584255009 6.244614020407399E-4 32-33 2.676263151603171E-4 0.0 0.0 0.031446092031337254 6.244614020407399E-4 34-35 3.3453289395039636E-4 0.0 0.0 0.05945764635145045 6.690657879007927E-4 36-37 4.0143947274047563E-4 0.0 0.0 0.10475340019233412 6.690657879007927E-4 38-39 4.0143947274047563E-4 0.0 0.0 0.17324343468044526 6.690657879007927E-4 40-41 4.0143947274047563E-4 0.0 0.0 0.24164426039683629 6.690657879007927E-4 42-43 4.0143947274047563E-4 0.0 0.0 0.31214149224864984 6.690657879007927E-4 44-45 4.0143947274047563E-4 0.0 0.0 0.39544018284229854 6.913679808308191E-4 46-47 4.0143947274047563E-4 0.0 0.0 0.4930122769111641 7.35972366690872E-4 48-49 4.0143947274047563E-4 0.0 0.0 0.597520352981268 7.805767525509248E-4 50-51 4.0143947274047563E-4 0.0 0.0 0.7123097399921139 8.92087717201057E-4 52-53 4.0143947274047563E-4 0.0 0.0 0.8179998322875092 0.0010259008747812155 54-55 4.0143947274047563E-4 0.0 0.0 0.9340158399095067 0.0010705052606412684 56-57 4.2374166567050206E-4 0.0 0.0 1.061093735224797 0.0010928074535712948 58-59 5.352526303206342E-4 0.0 0.0 1.1972263208696785 0.0011151096465013212 60-61 5.352526303206342E-4 0.0 0.0 1.3458927389412345 0.001159714032361374 62-63 5.352526303206342E-4 0.0 0.0 1.4908123886005464 0.001204318418221427 64-65 5.352526303206342E-4 0.0 0.0 1.6332118904587651 0.0012266206111514533 66-67 5.352526303206342E-4 0.0 0.0 1.8018387712026949 0.0012712249970115062 68-69 5.352526303206342E-4 0.0 0.0 1.9906937409341585 0.0012935271899415326 70-71 5.352526303206342E-4 0.0 0.0 2.1878674286285222 0.0012935271899415326 72-73 5.352526303206342E-4 0.0 0.0 2.387115220265378 0.0013604337687316119 74-75 5.352526303206342E-4 0.0 0.0 2.5831514961203106 0.001471944733381744 76-77 5.352526303206342E-4 0.0 0.0 2.7960482298303426 0.0015165491192417968 78-79 5.352526303206342E-4 0.0 0.0 3.0345478810240456 0.0015165491192417968 80-81 5.352526303206342E-4 0.0 0.0 3.2819907115826883 0.0015834556980318761 82-83 5.352526303206342E-4 0.0 0.0 3.53636952414257 0.001739571048542061 84-85 5.79857016180687E-4 0.0 0.0 3.7943389897641855 0.001739571048542061 86-87 5.79857016180687E-4 0.0 0.0 4.0603372448406105 0.0017618732414720875 88-89 5.79857016180687E-4 0.0 0.0 4.343842721367107 0.001784175434402114 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3915 0.0 58.298622 1 GTATCAA 7035 0.0 42.69221 1 TGGTATC 1530 0.0 33.853024 2 TATCAAC 8990 0.0 33.088497 2 ATCAACG 9045 0.0 32.83476 3 TCAACGC 9190 0.0 32.42011 4 CAACGCA 9300 0.0 32.087738 5 AACGCAG 9655 0.0 31.055569 6 GTGGTAT 1965 0.0 28.67356 1 ACGCAGA 11245 0.0 26.38218 7 CGCAGAG 11185 0.0 26.183653 8 TAAGGTA 890 0.0 26.161852 4 TAGCGCG 155 1.4068646E-6 24.52566 4 TACATGG 7070 0.0 24.464952 2 GTACATG 7305 0.0 24.054136 1 GCAGAGT 12325 0.0 23.376055 9 ACATGGG 7250 0.0 22.612236 3 GAGTACT 8540 0.0 21.68209 12-13 CAGAGTA 11950 0.0 21.08526 10-11 GTACTTT 8815 0.0 20.93795 14-15 >>END_MODULE