FastQCFastQC Report
Thu 26 May 2016
SRR938165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241932
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96890.432171894598052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88360.394124353459427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54690.24394138626862902No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA27920.12453544532126755No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA26230.1169973041109186No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23270.10379440589634296No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22890.10209943923366097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41100.059.4116061
GTATCAA71350.041.6802561
ATCAACG92250.032.1306723
TATCAAC93400.031.7393052
TCAACGC93850.031.4802574
CAACGCA94950.031.1155595
AACGCAG97900.030.2749966
TGGTATC16400.029.5471782
GTGGTAT19400.026.446731
CGCAGAG114450.025.6065778
ACGCAGA115500.025.3737937
GCAGAGT127650.022.6976839
TAAGGTG12550.022.330095
GAGTACT87450.022.07868812-13
GTACATG73000.021.930931
TACATGG71400.021.8905852
CAGAGTA122400.021.18769610-11
GTACTTT91600.021.00061614-15
GGACCGA5450.020.9168826
TATACGG1150.00372493420.651635
Produced by FastQC (version 0.11.5)