##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938165_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2241932 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.5150869874733 34.0 31.0 34.0 31.0 34.0 2 32.73349548514406 34.0 31.0 34.0 31.0 34.0 3 32.789208147258705 34.0 31.0 34.0 31.0 34.0 4 36.24686163541089 37.0 35.0 37.0 35.0 37.0 5 36.20787472590605 37.0 37.0 37.0 35.0 37.0 6 36.24526256817781 37.0 37.0 37.0 35.0 37.0 7 36.21180124999331 37.0 37.0 37.0 35.0 37.0 8 36.2015163707017 37.0 37.0 37.0 35.0 37.0 9 37.918055052517204 39.0 38.0 39.0 35.0 39.0 10-11 37.902904726815976 39.0 38.0 39.0 35.0 39.0 12-13 37.8471004026884 39.0 38.0 39.0 35.0 39.0 14-15 39.18456335874594 40.5 39.0 41.0 36.0 41.0 16-17 39.13263158739873 40.0 38.5 41.0 36.0 41.0 18-19 39.1601275150183 40.0 39.0 41.0 36.0 41.0 20-21 39.08453222488461 40.0 39.0 41.0 36.0 41.0 22-23 38.94050399387671 40.0 38.0 41.0 35.0 41.0 24-25 38.904078045186026 40.0 38.0 41.0 35.0 41.0 26-27 38.86871680318582 40.0 38.0 41.0 35.0 41.0 28-29 38.81850296976001 40.0 38.0 41.0 35.0 41.0 30-31 38.62050543905881 40.0 38.0 41.0 34.5 41.0 32-33 38.4477760699254 40.0 38.0 41.0 34.0 41.0 34-35 38.25259062273075 40.0 38.0 41.0 34.0 41.0 36-37 38.128096659488335 40.0 38.0 41.0 34.0 41.0 38-39 38.11820875923088 40.0 38.0 41.0 34.0 41.0 40-41 38.05490666086215 40.0 38.0 41.0 33.0 41.0 42-43 37.91297483598967 40.0 38.0 41.0 33.0 41.0 44-45 37.719197772278555 40.0 37.0 41.0 33.0 41.0 46-47 37.41086571760428 40.0 37.0 41.0 32.5 41.0 48-49 37.083172460181665 40.0 37.0 41.0 31.5 41.0 50-51 36.80199243331198 40.0 36.0 41.0 31.0 41.0 52-53 36.3915781120926 39.0 35.5 41.0 30.0 41.0 54-55 36.176767181163385 39.0 35.0 41.0 30.0 41.0 56-57 35.93118569162668 39.0 35.0 40.5 29.0 41.0 58-59 35.59775720227019 38.5 35.0 40.0 28.5 41.0 60-61 35.24205194448359 38.0 35.0 40.0 28.0 41.0 62-63 34.87438111414619 37.5 34.0 40.0 27.5 41.0 64-65 34.48521476119704 37.0 34.0 40.0 26.5 41.0 66-67 34.06794095449817 36.5 34.0 39.0 26.0 41.0 68-69 33.60474314118359 36.0 33.0 39.0 26.0 40.5 70-71 33.08229821421881 35.0 33.0 38.5 24.5 40.0 72-73 32.66592920748711 35.0 32.0 37.5 24.0 40.0 74-75 32.21649764578052 35.0 32.0 37.0 22.5 39.0 76-77 30.90862791556568 34.0 30.5 35.5 21.0 38.5 78-79 31.62491079122828 35.0 32.0 36.0 22.0 38.5 80-81 31.469743283917623 35.0 32.0 36.0 21.5 37.5 82-83 31.107211993940943 35.0 31.5 35.0 20.0 37.0 84-85 30.730763466510133 34.0 31.0 35.0 18.5 36.5 86-87 30.299100730976676 34.0 31.0 35.0 16.5 36.0 88-89 29.842914727119286 34.0 30.0 35.0 9.0 36.0 90-91 29.501037944058965 34.0 30.0 35.0 4.5 35.0 92-93 29.09998385321232 34.0 29.0 35.0 2.0 35.0 94-95 28.686394368785493 34.0 29.0 35.0 2.0 35.0 96-97 28.11493769659383 34.0 29.0 35.0 2.0 35.0 98-99 27.428460140628708 33.5 27.5 35.0 2.0 35.0 100-101 24.993018521525187 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10299601700452854 1101 2 0.1096405357209349 1101 3 0.0443529512435461 1101 4 -0.01587072666793432 1101 5 0.05171193834738119 1101 6 0.028256389198858756 1101 7 0.05069322160826317 1101 8 0.016767542401453284 1101 9 -0.050082198243295295 1101 10-11 0.01639800214339715 1101 12-13 0.08373960260160374 1101 14-15 0.058399979425281856 1101 16-17 0.04952141785060604 1101 18-19 0.08376389561452413 1101 20-21 0.11833295970799185 1101 22-23 0.16681097514083376 1101 24-25 0.09752199856085042 1101 26-27 0.0895752251681472 1101 28-29 0.06287053444619772 1101 30-31 -0.0242056596082989 1101 32-33 -0.07292185691937902 1101 34-35 -0.014226502506232919 1101 36-37 0.05559927012553345 1101 38-39 0.08394893005245763 1101 40-41 0.042784351802168885 1101 42-43 0.07206598004588471 1101 44-45 0.03458359834885272 1101 46-47 0.06329426075168954 1101 48-49 0.14651968449071973 1101 50-51 0.021018166431112206 1101 52-53 0.050840261843866585 1101 54-55 0.030176691749169038 1101 56-57 0.14035319025634863 1101 58-59 0.06936137176163726 1101 60-61 0.06890679690132373 1101 62-63 0.03707829018096476 1101 64-65 0.08557651287814139 1101 66-67 0.059946780134218614 1101 68-69 0.11973429044757466 1101 70-71 0.004306473149028989 1101 72-73 -0.02548048124519653 1101 74-75 0.031024725690670607 1101 76-77 0.0669468025421942 1101 78-79 0.03943380780922823 1101 80-81 0.23062860644610694 1101 82-83 0.3310912260136867 1101 84-85 0.4141676949014226 1101 86-87 0.4434790394930026 1101 88-89 0.3528881935335484 1101 90-91 0.361088332982316 1101 92-93 0.5738195267044155 1101 94-95 0.3346232560186202 1101 96-97 0.14775430834509606 1101 98-99 0.20414525051555543 1101 100-101 0.4290413519729128 1102 1 0.1557174640329535 1102 2 0.1285428613023285 1102 3 0.1410723310885018 1102 4 0.08409826558013123 1102 5 0.12498118899337385 1102 6 0.10267912821694836 1102 7 0.1186952887917272 1102 8 0.15592619873220315 1102 9 0.07613588961200435 1102 10-11 0.09871118043797367 1102 12-13 0.18260885324759357 1102 14-15 0.16869455306869696 1102 16-17 0.16118446694621724 1102 18-19 0.1576739731339032 1102 20-21 0.18837326978550806 1102 22-23 0.17303733172998648 1102 24-25 0.096978329310204 1102 26-27 0.06677212439295488 1102 28-29 0.054918079665831954 1102 30-31 0.05371061946146227 1102 32-33 -0.07522263211317437 1102 34-35 -0.0031401975966716122 1102 36-37 0.047285058239225464 1102 38-39 -0.10234564359095089 1102 40-41 -0.05713089367586832 1102 42-43 -0.028351591013553445 1102 44-45 -0.04035593653487268 1102 46-47 -0.10996465397698785 1102 48-49 -0.10804982455321266 1102 50-51 -0.015122402044340788 1102 52-53 -0.0661519862181521 1102 54-55 -0.15854888964617686 1102 56-57 -0.012779626281123058 1102 58-59 -0.19951718120993434 1102 60-61 -0.10546426253020513 1102 62-63 -0.06600181317819676 1102 64-65 0.11633000125022619 1102 66-67 -0.026734356816682237 1102 68-69 0.23990586667496672 1102 70-71 0.2846744318053638 1102 72-73 0.3455996221139799 1102 74-75 0.26321283938576556 1102 76-77 0.22416644078509051 1102 78-79 0.28246223158183525 1102 80-81 0.3866038002445613 1102 82-83 0.5320193913883635 1102 84-85 0.4412870747463842 1102 86-87 0.3146955769607054 1102 88-89 0.054626694825540056 1102 90-91 0.013985490605055162 1102 92-93 0.09760040525738845 1102 94-95 -0.14572609798654668 1102 96-97 0.05475379154923132 1102 98-99 0.13115506963700696 1102 100-101 0.3346971659264 1103 1 0.1809969311409958 1103 2 0.18597249784956205 1103 3 0.1733799182693332 1103 4 0.11658503565658407 1103 5 0.1138290784061482 1103 6 0.1368291609912191 1103 7 0.07777882395890146 1103 8 0.14986764034464528 1103 9 0.1674148961086459 1103 10-11 0.16894935702423908 1103 12-13 0.18742148311091 1103 14-15 0.2698319089061769 1103 16-17 0.225524160260008 1103 18-19 0.24733451265664286 1103 20-21 0.2787163746606538 1103 22-23 0.2615058494470759 1103 24-25 0.2619099841958459 1103 26-27 0.263096217659168 1103 28-29 0.29524650571619304 1103 30-31 0.3785225153835441 1103 32-33 0.4414432753039321 1103 34-35 0.3319209346468881 1103 36-37 0.28751490838867255 1103 38-39 0.29119542042138136 1103 40-41 0.43764142003592355 1103 42-43 0.4048121765852173 1103 44-45 0.47075454186828125 1103 46-47 0.5269511983945208 1103 48-49 0.4762547155060659 1103 50-51 0.45444826763908 1103 52-53 0.5119653026535289 1103 54-55 0.49038132772696486 1103 56-57 0.6347940652155373 1103 58-59 0.5044922891628758 1103 60-61 0.6161224678121897 1103 62-63 0.6411239970043496 1103 64-65 0.7330000845399027 1103 66-67 0.7479603615903088 1103 68-69 0.7620240717077849 1103 70-71 0.8425329178111198 1103 72-73 0.6263288560058697 1103 74-75 0.5758739584689962 1103 76-77 0.6469846739101364 1103 78-79 0.6317119664772015 1103 80-81 0.6276013782384702 1103 82-83 0.7564620389421854 1103 84-85 0.8900817660734752 1103 86-87 0.9000148540538895 1103 88-89 0.7458223104124286 1103 90-91 0.8335659039911327 1103 92-93 0.7739066308506786 1103 94-95 0.7350395798841092 1103 96-97 0.880294693588322 1103 98-99 0.8963839707245036 1103 100-101 0.9956724979082665 1104 1 0.0734278926119174 1104 2 0.07278766885990251 1104 3 0.1069931844796983 1104 4 0.0947181946148632 1104 5 0.06168122892075445 1104 6 0.022996136526359123 1104 7 0.05163160839154557 1104 8 3.0962209046947464E-4 1104 9 0.07336658698508103 1104 10-11 0.14128468824640805 1104 12-13 0.14549936936545294 1104 14-15 0.20366528554772856 1104 16-17 0.15597545089239162 1104 18-19 0.1261588421645783 1104 20-21 0.13019418419779072 1104 22-23 0.1883106835956454 1104 24-25 0.12413346600497022 1104 26-27 0.08286813090186484 1104 28-29 0.0984106705006198 1104 30-31 0.09238442785525791 1104 32-33 0.1947877640718687 1104 34-35 0.20008525648307796 1104 36-37 0.21355923125284448 1104 38-39 0.2974260923459511 1104 40-41 0.2651240991296717 1104 42-43 0.3363932881119638 1104 44-45 0.29946484227499326 1104 46-47 0.22059221993536227 1104 48-49 0.12387362035077842 1104 50-51 0.2795616066643447 1104 52-53 0.324851923651444 1104 54-55 0.27589525345956645 1104 56-57 0.1756779715974517 1104 58-59 0.33709509382188685 1104 60-61 0.2162370204192996 1104 62-63 0.21602484022955792 1104 64-65 0.17215299489951974 1104 66-67 0.17866864602343213 1104 68-69 0.2508312778139512 1104 70-71 0.16157644399451243 1104 72-73 0.1779072738568459 1104 74-75 0.2793197694224503 1104 76-77 0.22151240020983565 1104 78-79 0.3274163150978602 1104 80-81 0.31430285328770324 1104 82-83 0.38913437470173307 1104 84-85 0.4495791623455432 1104 86-87 0.30087806767570413 1104 88-89 0.24809480189118105 1104 90-91 0.36830932423595897 1104 92-93 0.5807917327296828 1104 94-95 0.27890080703902953 1104 96-97 0.5513787981410161 1104 98-99 0.6568445702434467 1104 100-101 0.6366600108650395 1105 1 0.044494766623316195 1105 2 0.05541009961236654 1105 3 0.08435197533625427 1105 4 0.043841380681868714 1105 5 0.039317427825878326 1105 6 0.03965840200763893 1105 7 0.036471080711649506 1105 8 0.02310020479852426 1105 9 0.007779588886926092 1105 10-11 -0.005755581266178922 1105 12-13 0.14946110305906046 1105 14-15 0.09803950367047776 1105 16-17 0.11107713605737501 1105 18-19 0.10350296240317647 1105 20-21 0.18634308474611316 1105 22-23 0.15024287511034373 1105 24-25 0.12220427974413894 1105 26-27 0.06127342583908302 1105 28-29 0.017207466437660912 1105 30-31 0.1436873565551693 1105 32-33 0.09695408086653146 1105 34-35 0.3150588893815396 1105 36-37 0.32602952325149204 1105 38-39 0.2794865939743829 1105 40-41 0.27324717583388036 1105 42-43 0.27088964579763086 1105 44-45 0.34947008813232117 1105 46-47 0.30399557212710704 1105 48-49 0.44340812608876234 1105 50-51 0.3191551594167805 1105 52-53 0.25912266501556047 1105 54-55 0.30638724374672677 1105 56-57 0.3217802106894041 1105 58-59 0.4376895205874973 1105 60-61 0.36581219488851957 1105 62-63 0.44776325510930093 1105 64-65 0.3276891081815805 1105 66-67 0.4183989911741719 1105 68-69 0.41009684000537305 1105 70-71 0.5439620998644941 1105 72-73 0.30603400487862586 1105 74-75 0.2809166439584416 1105 76-77 0.29195381687583577 1105 78-79 0.38666894047162614 1105 80-81 0.44442878942872355 1105 82-83 0.6667351418416807 1105 84-85 0.7006996863622348 1105 86-87 0.7148769046958101 1105 88-89 0.3638877665741127 1105 90-91 0.2265291381058283 1105 92-93 0.37346100276419136 1105 94-95 0.3507292639478692 1105 96-97 0.6002488493636982 1105 98-99 0.710085354205706 1105 100-101 0.5626307389811451 1106 1 -0.0808948254863111 1106 2 -0.04550305402265309 1106 3 -0.015567927877334853 1106 4 -0.08133959113679623 1106 5 -0.0758309187954751 1106 6 -0.10796558882312013 1106 7 -0.04799835348331527 1106 8 -0.10260022316631279 1106 9 -0.19877963414072752 1106 10-11 -0.11160273045733504 1106 12-13 -0.23399995783795902 1106 14-15 -0.3602410462157408 1106 16-17 -0.27420788617870073 1106 18-19 -0.15470570145506457 1106 20-21 -0.14008315750812983 1106 22-23 -0.03371686368700466 1106 24-25 -0.12958093184207797 1106 26-27 -0.18694199094907304 1106 28-29 -0.03581224943658157 1106 30-31 0.02948261877997993 1106 32-33 1.766778791534307E-4 1106 34-35 -0.11868950616923257 1106 36-37 -0.028376554050289826 1106 38-39 -0.02414264503911312 1106 40-41 -0.0013687617509461347 1106 42-43 -0.20005453277463658 1106 44-45 -0.16783911227386028 1106 46-47 -0.19005665499922486 1106 48-49 -0.025160168989138754 1106 50-51 -0.11498219986925307 1106 52-53 -0.11776413376053796 1106 54-55 -0.5372214767490036 1106 56-57 -0.2582991906960359 1106 58-59 -0.17807086267059447 1106 60-61 -0.19494448514996066 1106 62-63 -0.0704510138483343 1106 64-65 -0.3851234871218665 1106 66-67 -0.3959136594262205 1106 68-69 -0.5367378707245862 1106 70-71 -0.5808872997447452 1106 72-73 -0.42037242263712926 1106 74-75 -0.5047639189979698 1106 76-77 -0.5500825014871076 1106 78-79 -0.5365900017145826 1106 80-81 -0.7140340309165296 1106 82-83 -0.9735450377225767 1106 84-85 -0.8135216823879539 1106 86-87 -0.395639275525312 1106 88-89 -0.32955246580710806 1106 90-91 -0.7146175278235454 1106 92-93 -0.5882764439915569 1106 94-95 -0.5614968905015232 1106 96-97 -0.5423548491640595 1106 98-99 -0.3595408300705216 1106 100-101 -0.2490439960124391 1107 1 0.09514598811949071 1107 2 0.06366572347367594 1107 3 0.06517051334637358 1107 4 0.0525544610848101 1107 5 0.06987346347736434 1107 6 0.08387787966679383 1107 7 0.08775180046441733 1107 8 0.08996060999243838 1107 9 0.08734888205710689 1107 10-11 0.1035947670191959 1107 12-13 0.10146126637976494 1107 14-15 0.1322543988360252 1107 16-17 0.12568363622148837 1107 18-19 0.09747654726097466 1107 20-21 0.12538541493618283 1107 22-23 0.13804713920785616 1107 24-25 0.13534683969314898 1107 26-27 0.1237737231461935 1107 28-29 0.21997983542828337 1107 30-31 0.18866655298557333 1107 32-33 0.2917429611048803 1107 34-35 0.4165304010177451 1107 36-37 0.35739661270207534 1107 38-39 0.2713417665631468 1107 40-41 0.1982617637028028 1107 42-43 0.2551042238355947 1107 44-45 0.3226073996197911 1107 46-47 0.16907958715260918 1107 48-49 0.29871252100140566 1107 50-51 0.34710817220812373 1107 52-53 0.30629202383115484 1107 54-55 0.40211002508250004 1107 56-57 0.4498123468132533 1107 58-59 0.43879926893090015 1107 60-61 0.39884856466562724 1107 62-63 0.4200673138667028 1107 64-65 0.2595544447094511 1107 66-67 0.2951271383086933 1107 68-69 0.4549874389166746 1107 70-71 0.5383019670831715 1107 72-73 0.32283205486110944 1107 74-75 0.30973264019096547 1107 76-77 0.20462560092463278 1107 78-79 0.39435928441234935 1107 80-81 0.49059463764195144 1107 82-83 0.45971029013847087 1107 84-85 0.4873667705802198 1107 86-87 0.6281817546865334 1107 88-89 0.53541355459652 1107 90-91 0.576755923970186 1107 92-93 0.6683091858609735 1107 94-95 0.4882583802242806 1107 96-97 0.5784703106558986 1107 98-99 0.6168785838488908 1107 100-101 0.5412073831687536 1108 1 -0.04979620712354205 1108 2 -0.05617398686339925 1108 3 -0.027384727429982547 1108 4 -0.12388902283716874 1108 5 -0.07203283009172878 1108 6 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-0.4974325800465955 2316 100-101 -0.2996207450749324 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 2.0 4 10.0 5 33.0 6 65.0 7 164.0 8 431.0 9 858.0 10 1438.0 11 2467.0 12 3567.0 13 5348.0 14 7320.0 15 9850.0 16 12605.0 17 17763.0 18 17617.0 19 13272.0 20 11507.0 21 11230.0 22 11724.0 23 12364.0 24 14228.0 25 16602.0 26 19581.0 27 24150.0 28 29237.0 29 36096.0 30 43794.0 31 54226.0 32 68433.0 33 89692.0 34 125581.0 35 191498.0 36 318145.0 37 535420.0 38 479396.0 39 56206.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15920375818714 18.603864880826002 14.535365033373001 23.70156632761386 2 16.00354931545411 19.579676466245143 39.60859384026921 24.808180378031537 3 19.357292133653615 23.030424687690278 29.9027774989796 27.709505679676504 4 13.18291544971034 16.295900143269286 35.82807150261471 34.693112904405666 5 13.680171245234577 37.06933888687921 34.502979797326496 14.747510070559711 6 33.61404360167926 35.39259888346301 16.469500413036613 14.523857101821108 7 29.22773750497339 32.49688215342838 19.86121791383503 18.4141624277632 8 26.905550768001056 35.14396562952179 19.000911716900305 18.94957188557685 9 26.330520718151334 16.30800879423602 19.75107976500531 37.610390722607335 10-11 25.45581221910388 26.18058888494388 26.651722710590686 21.711876185361554 12-13 26.018518848921378 23.44725442163277 27.340927378707292 23.19329935073856 14-15 22.78869296660202 25.025156873625072 25.499346099703292 26.68680406006962 16-17 22.32119957518688 28.176274249786008 26.662353412934497 22.84017276209261 18-19 22.385893060092812 26.523418194664245 28.965329902958697 22.125358842284243 20-21 22.664068312509034 25.69154639837426 29.570098468642225 22.074286820474484 22-23 22.483821989248558 25.58195342231611 29.433140701858935 22.5010838865764 24-25 22.40541740704667 25.93018009327729 29.404804732603402 22.25959776707264 26-27 21.965140108549857 26.28734817084501 29.163377327753338 22.584134392851798 28-29 22.114274652398024 26.700118469248846 29.07385237375621 22.111754504596927 30-31 22.169851717179647 26.17554858934169 29.07155524788441 22.583044445594247 32-33 21.951115616173173 26.04851416585542 29.1702322448702 22.830137973101213 34-35 22.638487961317036 26.301832676147953 28.828440374953683 22.231238987581325 36-37 22.665693065343177 25.85437865225951 28.930478576050078 22.54944970634724 38-39 22.238798179638668 26.198632810670198 28.964797752252586 22.59777125743855 40-41 22.600595784451386 26.016954539928822 29.00845719486122 22.37399248075857 42-43 22.232900916831234 26.03380494552676 28.81342776580545 22.91986637183656 44-45 22.365669002069254 26.354773628610772 28.955728456925893 22.323828912394077 46-47 22.73575770173123 26.583556764235833 28.233005386874083 22.447680147158856 48-49 22.480855797588863 26.623867271621084 28.2151510393714 22.68012589141865 50-51 22.677895672125647 26.845907904432426 28.13515307333139 22.341043350110528 52-53 22.853235512941517 26.566193800704035 28.03055578848957 22.550014897864877 54-55 22.3988060297993 26.777194848015018 28.171862482894216 22.652136639291466 56-57 22.57744659516881 26.993905256716083 27.8898289511011 22.538819197014003 58-59 22.52864939703791 26.617801075144115 27.984635573246646 22.868913954571322 60-61 22.32663613347773 26.81613447687084 28.178776162702523 22.6784532269489 62-63 22.287183554184516 26.84956546407295 28.381748420558694 22.481502561183834 64-65 22.152143775993206 26.824297079483234 28.19630568634553 22.827253458178035 66-67 22.116705591427394 26.903380655613105 28.03552917751297 22.944384575446534 68-69 22.469420279964513 26.615897841455137 28.209571498090707 22.70511038048964 70-71 22.313968487893476 26.8172941909032 27.839091462185294 23.029645859018025 72-73 22.255068396365278 26.837031631646273 27.873102306403585 23.03479766558486 74-75 22.30761236290842 27.222770360564013 27.523381619067838 22.946235657459727 76-77 22.413659290290695 26.841046026373682 27.798233844737485 22.947060838598137 78-79 22.340218168972118 26.893612295109754 27.858851205121297 22.90731833079683 80-81 22.483465154161678 27.044219003966223 27.587232797426502 22.885083044445594 82-83 22.398855629621156 27.181829596488566 27.59009363131746 22.829221142572823 84-85 22.324520505142036 27.268084221860867 27.508732128993525 22.89866314400357 86-87 22.488962060085377 27.172523274068084 27.44385241889063 22.89466224695591 88-89 22.32394015958129 27.270795089676103 27.290331832468418 23.114932918274192 90-91 22.34289467433715 27.187163724072256 27.365470114443934 23.104471487146665 92-93 22.378006039008007 26.865958242572397 27.54421480295166 23.211820915467936 94-95 22.165079047892622 26.81452871897988 27.643233603873803 23.377158629253696 96-97 22.426849889671 26.489419165888688 27.573194714734754 23.510536229705554 98-99 22.041346481516836 26.799764667260202 27.358501506736154 23.800387344486808 100-101 22.219942442500486 26.48376489563466 27.649745844209367 23.646546817655487 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1102.0 1 741.5 2 530.0 3 720.5 4 585.5 5 515.0 6 1091.5 7 2354.0 8 3774.0 9 4415.0 10 3597.0 11 2544.0 12 2342.5 13 2388.5 14 2442.5 15 2525.5 16 2653.0 17 2838.5 18 3103.5 19 3364.5 20 3782.5 21 4661.5 22 5671.5 23 6864.5 24 8655.0 25 11043.0 26 13870.5 27 16739.5 28 18966.0 29 21346.0 30 24878.5 31 28995.5 32 34970.0 33 38018.5 34 38591.5 35 41149.5 36 45105.0 37 49928.0 38 53634.0 39 56373.5 40 59048.0 41 63274.0 42 66084.5 43 67452.0 44 70103.0 45 74895.5 46 78169.0 47 80784.5 48 82177.0 49 82161.5 50 83510.0 51 83150.0 52 84672.5 53 88151.0 54 89895.0 55 83540.0 56 74221.0 57 67707.5 58 61851.5 59 53662.0 60 42487.0 61 34853.5 62 28544.5 63 20554.5 64 14163.0 65 10365.5 66 7604.0 67 5947.5 68 4553.0 69 3467.5 70 2692.5 71 1761.0 72 1201.5 73 962.0 74 724.5 75 468.5 76 312.0 77 199.0 78 114.0 79 56.0 80 27.0 81 15.5 82 9.5 83 8.0 84 5.0 85 1.5 86 1.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016637435925799714 3 0.007448932438628825 4 0.0 5 4.460438586005285E-5 6 0.0 7 0.0 8 3.568350868804228E-4 9 4.0143947274047563E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.2302192930026421E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00541943288199642 26-27 0.02112017670473502 28-29 0.0 30-31 0.0 32-33 4.2374166567050206E-4 34-35 0.019112979341032645 36-37 0.0037244662193144126 38-39 0.017797149958161087 40-41 0.01558923285808847 42-43 0.008430228927549988 44-45 0.0028100763091833294 46-47 4.460438586005285E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.2302192930026421E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.2302192930026424E-5 84-85 0.003010796045553567 86-87 2.4532412223029067E-4 88-89 1.1151096465013211E-4 90-91 2.0071973637023782E-4 92-93 3.3453289395039636E-4 94-95 0.0 96-97 4.460438586005285E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2241932.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.18718393888676 #Duplication Level Percentage of deduplicated Percentage of total 1 75.5308580353375 40.172736393874395 2 13.985877326006419 14.877388597700184 3 4.544442573881601 7.251183092302462 4 1.9685919714255546 4.188154531393068 5 1.066679749244477 2.836684601347581 6 0.6130857809081114 1.9564983719685451 7 0.4140645399262234 1.5416048793338595 8 0.2855725802437239 1.2151041082660354 9 0.21489422351726664 1.0286656734255395 >10 1.2155372562104028 12.307987537140457 >50 0.10541581732952622 3.85706264656896 >100 0.04911747088140128 4.643878209178578 >500 0.0035176050527169537 1.301488704510515 >1k 0.0020938125313790445 1.752735601066965 >5k 2.512575037654854E-4 1.0688270519226561 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9689 0.432171894598052 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8836 0.394124353459427 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5469 0.24394138626862902 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2792 0.12453544532126755 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2623 0.1169973041109186 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2327 0.10379440589634296 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2289 0.10209943923366097 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.460438586005285E-5 2 0.0 0.0 0.0 0.0 4.460438586005285E-5 3 0.0 0.0 0.0 0.0 4.460438586005285E-5 4 0.0 0.0 0.0 0.0 4.460438586005285E-5 5 0.0 0.0 0.0 0.0 8.92087717201057E-5 6 0.0 0.0 0.0 0.0 8.92087717201057E-5 7 0.0 0.0 0.0 4.460438586005285E-5 8.92087717201057E-5 8 0.0 0.0 0.0 4.460438586005285E-5 8.92087717201057E-5 9 0.0 0.0 0.0 4.460438586005285E-5 8.92087717201057E-5 10-11 8.92087717201057E-5 0.0 0.0 4.460438586005285E-5 8.92087717201057E-5 12-13 8.92087717201057E-5 0.0 0.0 4.460438586005285E-5 3.3453289395039636E-4 14-15 1.1151096465013212E-4 0.0 0.0 4.460438586005285E-5 4.2374166567050206E-4 16-17 1.3381315758015854E-4 0.0 0.0 8.92087717201057E-5 5.575548232506606E-4 18-19 1.3381315758015854E-4 0.0 0.0 8.92087717201057E-5 7.35972366690872E-4 20-21 1.3381315758015854E-4 0.0 0.0 1.3381315758015854E-4 8.251811384109777E-4 22-23 1.3381315758015854E-4 0.0 0.0 3.3453289395039636E-4 8.474833313410041E-4 24-25 1.784175434402114E-4 0.0 0.0 5.575548232506606E-4 8.474833313410041E-4 26-27 2.2302192930026424E-4 0.0 0.0 8.92087717201057E-4 8.697855242710305E-4 28-29 2.2302192930026424E-4 0.0 0.0 0.003278422360713884 8.92087717201057E-4 30-31 2.2302192930026424E-4 0.0 0.0 0.012511530233744825 8.92087717201057E-4 32-33 2.2302192930026424E-4 0.0 0.0 0.03128997668082707 9.366921030611098E-4 34-35 2.4532412223029067E-4 0.0 0.0 0.059368437579730336 9.366921030611098E-4 36-37 2.676263151603171E-4 0.0 0.0 0.10515483966507458 9.366921030611098E-4 38-39 2.676263151603171E-4 0.0 0.0 0.17415782459057633 9.366921030611098E-4 40-41 3.1223070102036994E-4 0.0 0.0 0.24287088100798776 9.366921030611098E-4 42-43 3.1223070102036994E-4 0.0 0.0 0.3138141567184018 9.812964889211627E-4 44-45 3.1223070102036994E-4 0.0 0.0 0.39764809994237116 0.0010928074535712948 46-47 3.1223070102036994E-4 0.0 0.0 0.49555472690518715 0.0011820162252914005 48-49 3.1223070102036994E-4 0.0 0.0 0.6007095665702618 0.001204318418221427 50-51 3.3453289395039636E-4 0.0 0.0 0.715788882089198 0.001204318418221427 52-53 4.0143947274047563E-4 0.0 0.0 0.8221480401724941 0.001204318418221427 54-55 4.0143947274047563E-4 0.0 0.0 0.9384316741096519 0.001204318418221427 56-57 4.0143947274047563E-4 0.0 0.0 1.0662232395987032 0.001204318418221427 58-59 4.0143947274047563E-4 0.0 0.0 1.2026457537516748 0.001204318418221427 60-61 4.0143947274047563E-4 0.0 0.0 1.3515797981383915 0.001204318418221427 62-63 4.0143947274047563E-4 0.0 0.0 1.4970793048138837 0.0012935271899415326 64-65 4.0143947274047563E-4 0.0 0.0 1.6399694549165629 0.0012935271899415326 66-67 4.0143947274047563E-4 0.0 0.0 1.809555329956484 0.001315829382871559 68-69 4.460438586005285E-4 0.0 0.0 2.0001275685435598 0.0013381315758015854 70-71 4.460438586005285E-4 0.0 0.0 2.1985947834278647 0.0013827359616616383 72-73 4.460438586005285E-4 0.0 0.0 2.399537541727403 0.0014942469263117704 74-75 4.460438586005285E-4 0.0 0.0 2.597335690823807 0.0016057578909619025 76-77 4.460438586005285E-4 0.0 0.0 2.8112583254086205 0.0016503622768219554 78-79 4.460438586005285E-4 0.0 0.0 3.0514975476508654 0.0016726644697519818 80-81 4.460438586005285E-4 0.0 0.0 3.301884267676272 0.0016949666626820082 82-83 4.460438586005285E-4 0.0 0.0 3.5596084091756577 0.001784175434402114 84-85 4.460438586005285E-4 0.0 0.0 3.8214584563670977 0.001784175434402114 86-87 4.460438586005285E-4 0.0 0.0 4.091515710556788 0.0018287798202621668 88-89 4.460438586005285E-4 0.0 0.0 4.379838460756169 0.0018733842061222196 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4110 0.0 59.411606 1 GTATCAA 7135 0.0 41.680256 1 ATCAACG 9225 0.0 32.130672 3 TATCAAC 9340 0.0 31.739305 2 TCAACGC 9385 0.0 31.480257 4 CAACGCA 9495 0.0 31.115559 5 AACGCAG 9790 0.0 30.274996 6 TGGTATC 1640 0.0 29.547178 2 GTGGTAT 1940 0.0 26.44673 1 CGCAGAG 11445 0.0 25.606577 8 ACGCAGA 11550 0.0 25.373793 7 GCAGAGT 12765 0.0 22.697683 9 TAAGGTG 1255 0.0 22.33009 5 GAGTACT 8745 0.0 22.078688 12-13 GTACATG 7300 0.0 21.93093 1 TACATGG 7140 0.0 21.890585 2 CAGAGTA 12240 0.0 21.187696 10-11 GTACTTT 9160 0.0 21.000616 14-15 GGACCGA 545 0.0 20.916882 6 TATACGG 115 0.003724934 20.65163 5 >>END_MODULE