FastQCFastQC Report
Thu 26 May 2016
SRR938164_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938164_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199900
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92270.41942815582526477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82440.37474430655938906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51220.2328287649438611No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28210.12823310150461384No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25350.11523251056866221No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23180.10536842583753805No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23020.1046411200509114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40500.059.3705441
GTATCAA71550.043.1221121
ATCAACG90100.033.8875243
TATCAAC91350.033.423822
TCAACGC91250.033.3562134
CAACGCA92800.032.8503235
AACGCAG95750.031.8820846
TGGTATC15550.031.5018772
GTGGTAT18300.028.620441
CGCAGAG111100.027.3022028
ACGCAGA112300.027.0969877
GCAGAGT122800.024.4675249
TACATGG68500.022.7031862
GAGTACT84050.022.5956912-13
GTACATG71500.022.5084381
CAGAGTA118700.022.26771210-11
GTACTTT86800.021.68466814-15
ACATGGG71150.020.9886443
AGAGTAC115900.020.7958210-11
AGTACTT87050.020.28787612-13
Produced by FastQC (version 0.11.5)