##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938164_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2199900 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.981256420746398 33.0 31.0 34.0 30.0 34.0 2 32.10104822946498 34.0 31.0 34.0 30.0 34.0 3 32.12550752306923 34.0 31.0 34.0 30.0 34.0 4 35.63118414473385 37.0 35.0 37.0 33.0 37.0 5 35.63781808264012 37.0 35.0 37.0 35.0 37.0 6 35.66345242965589 37.0 35.0 37.0 35.0 37.0 7 35.59571025955725 37.0 35.0 37.0 33.0 37.0 8 35.58587663075594 37.0 35.0 37.0 33.0 37.0 9 37.226783490158645 39.0 37.0 39.0 34.0 39.0 10-11 37.13526273921542 39.0 37.0 39.0 34.0 39.0 12-13 36.99137824446566 39.0 37.0 39.0 33.0 39.0 14-15 38.183998590845036 40.0 38.0 41.0 33.0 41.0 16-17 38.008042183735625 40.0 38.0 41.0 33.0 41.0 18-19 38.17362357379881 40.0 38.0 41.0 33.0 41.0 20-21 38.1739824537479 40.0 38.0 41.0 33.5 41.0 22-23 38.06360152734215 40.0 38.0 41.0 33.0 41.0 24-25 37.94605232056003 40.0 38.0 41.0 33.0 41.0 26-27 37.76889449520433 40.0 38.0 41.0 32.5 41.0 28-29 37.59408791308695 40.0 37.5 41.0 32.0 41.0 30-31 37.36626573935179 40.0 37.0 41.0 31.5 41.0 32-33 37.07755988908587 40.0 36.5 41.0 31.0 41.0 34-35 36.842570116823495 39.5 36.0 41.0 30.0 41.0 36-37 36.58367880358198 39.0 35.5 41.0 30.0 41.0 38-39 36.291823719259966 39.0 35.0 41.0 30.0 41.0 40-41 36.181261648256736 39.0 35.0 41.0 29.5 41.0 42-43 35.97509295877086 39.0 35.0 41.0 28.5 41.0 44-45 35.66787058502659 39.0 35.0 40.5 27.5 41.0 46-47 35.293723351061416 39.0 35.0 40.0 26.0 41.0 48-49 34.89352516023455 38.0 34.5 40.0 24.0 41.0 50-51 34.1641874630665 37.5 33.5 39.5 23.5 40.5 52-53 34.22988954043366 38.0 34.0 40.0 24.0 40.5 54-55 34.31944179280876 38.0 34.0 40.0 23.0 41.0 56-57 34.0030346833947 38.0 33.5 40.0 22.0 41.0 58-59 33.59952702395563 37.5 33.0 40.0 20.0 41.0 60-61 33.09231715077958 37.0 32.5 40.0 17.0 41.0 62-63 32.55464043820173 36.5 32.0 39.5 12.5 41.0 64-65 32.01490317741715 36.0 31.0 39.0 8.0 40.5 66-67 31.4506009364062 35.0 31.0 39.0 4.5 40.0 68-69 30.77647802172826 35.0 30.0 38.0 2.0 40.0 70-71 30.187337378971772 35.0 29.0 37.5 2.0 39.5 72-73 29.503584026546662 34.0 28.5 37.0 2.0 39.0 74-75 28.835757534433384 34.0 27.5 36.0 2.0 39.0 76-77 28.099937269875902 34.0 26.0 35.5 2.0 38.5 78-79 27.350395699804537 33.0 25.5 35.0 2.0 37.0 80-81 26.649768853129686 32.5 24.5 35.0 2.0 37.0 82-83 25.927036001636438 32.0 21.0 35.0 2.0 36.0 84-85 25.219414518841766 31.5 19.0 35.0 2.0 36.0 86-87 24.496111641438247 31.0 14.5 34.0 2.0 35.0 88-89 23.632726714850676 31.0 4.5 34.0 2.0 35.0 90-91 22.758635392517842 30.0 2.0 34.0 2.0 35.0 92-93 21.8406009364062 29.0 2.0 34.0 2.0 35.0 94-95 20.772575117050778 29.0 2.0 34.0 2.0 35.0 96-97 19.541110050456837 26.5 2.0 34.0 2.0 35.0 98-99 17.999994999772717 24.5 2.0 33.0 2.0 35.0 100-101 14.01191531433247 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.175599497289312 1101 2 0.27324568160894813 1101 3 0.20817855028808907 1101 4 0.0317813451061042 1101 5 0.22816339175090405 1101 6 0.229592903159336 1101 7 0.34856201776933915 1101 8 0.25680449730563737 1101 9 0.33171536485491515 1101 10-11 0.37476070070868417 1101 12-13 0.36807526506154176 1101 14-15 0.4756981449472235 1101 16-17 0.5131683566361147 1101 18-19 0.5416113056918448 1101 20-21 0.4653809193137306 1101 22-23 0.3660071852433475 1101 24-25 0.4585351391041854 1101 26-27 0.48368708308019137 1101 28-29 0.4167876192028288 1101 30-31 0.4610202004851871 1101 32-33 0.47215763646611464 1101 34-35 0.3488470695692669 1101 36-37 0.24798600463461895 1101 38-39 0.3044151462784228 1101 40-41 0.33968709108237505 1101 42-43 0.4925584571121888 1101 44-45 0.5639687321203795 1101 46-47 0.47579280876119867 1101 48-49 0.5589362350145848 1101 50-51 0.6249813283370287 1101 52-53 0.6253608085325979 1101 54-55 0.6520220285224099 1101 56-57 0.5745372222527365 1101 58-59 0.7138494184061628 1101 60-61 0.6742212042322038 1101 62-63 0.5228041785027315 1101 64-65 0.5169739177224599 1101 66-67 0.45132516864691397 1101 68-69 0.3033748028028427 1101 70-71 0.37779220466686425 1101 72-73 0.17147220804266894 1101 74-75 0.34964026434279916 1101 76-77 0.09014413736789706 1101 78-79 0.014046517501839162 1101 80-81 -0.06380235225718067 1101 82-83 -0.12202144734810005 1101 84-85 -0.2454199784861224 1101 86-87 0.05205947015398493 1101 88-89 -0.10282641392339187 1101 90-91 -0.12760807195307322 1101 92-93 -0.42994171325859654 1101 94-95 -0.6029569837567159 1101 96-97 -0.46240295379647023 1101 98-99 -0.6631592878071828 1101 100-101 -0.7934852903726011 1102 1 0.13691370911487155 1102 2 0.07737757299251768 1102 3 0.15867813886220716 1102 4 0.02828716384361485 1102 5 0.029209589000231517 1102 6 0.08220839628264542 1102 7 0.04478571590028935 1102 8 0.10359831416234044 1102 9 0.0971438355261327 1102 10-11 0.16837919136343515 1102 12-13 0.18985585751482148 1102 14-15 0.13712549889103798 1102 16-17 0.16416018689354672 1102 18-19 0.13844038762437094 1102 20-21 0.16004707659596562 1102 22-23 0.26342842657402343 1102 24-25 0.161240264295202 1102 26-27 0.19837645653733915 1102 28-29 0.23425705613712466 1102 30-31 0.28585122199688584 1102 32-33 0.13571999687518854 1102 34-35 0.14432432359536307 1102 36-37 0.18428111454411322 1102 38-39 0.10074023150234979 1102 40-41 0.1585818248310531 1102 42-43 0.21962293342581773 1102 44-45 0.21231862044763972 1102 46-47 0.23444597674050982 1102 48-49 0.27284977680370304 1102 50-51 0.16095170567334094 1102 52-53 0.17454383427610765 1102 54-55 0.01613834449749163 1102 56-57 0.06966476427695056 1102 58-59 -0.03134130687889325 1102 60-61 -0.0997267210058439 1102 62-63 7.781998968781068E-4 1102 64-65 -0.1823090257358686 1102 66-67 -0.2592646397461813 1102 68-69 -0.3583449573946389 1102 70-71 -0.46683574878323597 1102 72-73 -0.30098870649829834 1102 74-75 -0.32790763738519146 1102 76-77 -0.4714992151653128 1102 78-79 -0.38303823623038014 1102 80-81 -0.3592987081993435 1102 82-83 -0.42516444723054647 1102 84-85 -0.3650258912638371 1102 86-87 -0.35635125042906424 1102 88-89 -0.35437073036867517 1102 90-91 -0.3386166531215231 1102 92-93 -0.5274863823546347 1102 94-95 -0.7630530810883265 1102 96-97 -0.8722272161686355 1102 98-99 -0.9187928836694113 1102 100-101 -1.1563438186434372 1103 1 0.23023455669429893 1103 2 0.23124089754226418 1103 3 0.24627804304130052 1103 4 0.12243827776160288 1103 5 0.21300932331499922 1103 6 0.10378552122003981 1103 7 0.12643655759980277 1103 8 0.10504270537295213 1103 9 0.2123031377757414 1103 10-11 0.33091947969724345 1103 12-13 0.12004515824001061 1103 14-15 0.26162162250000165 1103 16-17 0.12653659970741415 1103 18-19 0.041140250426259684 1103 20-21 0.2349599666850395 1103 22-23 0.13519038785366888 1103 24-25 0.1430089765929452 1103 26-27 0.08836398893134145 1103 28-29 -0.034109251927077366 1103 30-31 -0.021394961500149634 1103 32-33 0.0845249577846161 1103 34-35 -0.049839856809256844 1103 36-37 -0.1466344650634639 1103 38-39 -0.03231444085549384 1103 40-41 0.21515998419863536 1103 42-43 0.2947729258036915 1103 44-45 0.35441530156003864 1103 46-47 0.30645946788796863 1103 48-49 0.11658847973713193 1103 50-51 0.17244368067279936 1103 52-53 0.18352595496688195 1103 54-55 0.06163285657309814 1103 56-57 0.16461565606466166 1103 58-59 0.06876948603906641 1103 60-61 -0.014864790214666357 1103 62-63 -0.01837781395359883 1103 64-65 -0.12984490413042948 1103 66-67 -0.18192437754534652 1103 68-69 -0.25435859009570194 1103 70-71 -0.42674559002542267 1103 72-73 -0.4332406065832295 1103 74-75 -0.5246159716259164 1103 76-77 -0.4068005065697413 1103 78-79 -0.407672919041417 1103 80-81 -0.5581785291884778 1103 82-83 -0.6024050774707526 1103 84-85 -0.8013324854840924 1103 86-87 -1.2299112147140008 1103 88-89 -1.0163889036738425 1103 90-91 -1.0690779000998987 1103 92-93 -1.407506363190457 1103 94-95 -1.5320052033035338 1103 96-97 -1.6092930277063395 1103 98-99 -1.6011482267735744 1103 100-101 -1.53553135539293 1104 1 0.11978310613782384 1104 2 0.1362351123079648 1104 3 0.23653113692796524 1104 4 0.14817195065717215 1104 5 0.24513760178300714 1104 6 0.29287823061113016 1104 7 0.19879294826090188 1104 8 0.22836073952606029 1104 9 0.2357045116677483 1104 10-11 0.3176149579095622 1104 12-13 0.305474813027395 1104 14-15 0.201576554311103 1104 16-17 0.23791449687588795 1104 18-19 0.3336657681642734 1104 20-21 0.2263640390045154 1104 22-23 0.33371267516539405 1104 24-25 0.352580353438249 1104 26-27 0.2928050159520197 1104 28-29 0.08046847653910305 1104 30-31 0.28836362633629875 1104 32-33 0.42698124487787226 1104 34-35 0.27570625110356417 1104 36-37 0.11515320267799467 1104 38-39 0.11302262995496193 1104 40-41 0.26616739374872367 1104 42-43 0.21311591130824326 1104 44-45 0.08764975323715163 1104 46-47 0.03601544104123633 1104 48-49 -0.02984343655093369 1104 50-51 -0.0729604968327493 1104 52-53 0.1343127771740953 1104 54-55 0.18088479139009905 1104 56-57 0.08582824926559596 1104 58-59 0.1397660432178185 1104 60-61 -0.07651799171477336 1104 62-63 -0.059645650073690604 1104 64-65 -0.13842920065808073 1104 66-67 -0.06882228838800941 1104 68-69 0.05789841994957001 1104 70-71 -0.028812243611255894 1104 72-73 -0.19455316701465364 1104 74-75 -0.44536306586050856 1104 76-77 -0.3777013848734967 1104 78-79 -0.3829688560268636 1104 80-81 -0.583339999932587 1104 82-83 -0.6840661375845976 1104 84-85 -0.5263548375257194 1104 86-87 -0.4352344393955079 1104 88-89 -0.477581562090144 1104 90-91 -0.529697092108993 1104 92-93 -0.9475036222839961 1104 94-95 -1.2318309382912567 1104 96-97 -1.3932294447448683 1104 98-99 -1.4867371272149512 1104 100-101 -1.5131651541072326 1105 1 0.20110151598884585 1105 2 0.14589230294341604 1105 3 0.18766750056433068 1105 4 0.05991564174868813 1105 5 0.009764007985502587 1105 6 0.09393098220736107 1105 7 0.1520177126269715 1105 8 0.09700052365497669 1105 9 0.13165692881582203 1105 10-11 0.1708853288429779 1105 12-13 0.14651502281760287 1105 14-15 0.1888542222952907 1105 16-17 0.23479234728634424 1105 18-19 0.16572148418737953 1105 20-21 0.16519233027845814 1105 22-23 0.07281365570659659 1105 24-25 0.06916784203661308 1105 26-27 0.1144310801052626 1105 28-29 0.1987415078556225 1105 30-31 0.1648610419641514 1105 32-33 0.06593685448566333 1105 34-35 0.08709744143497034 1105 36-37 0.2269928575883 1105 38-39 -0.022270202212865797 1105 40-41 0.044020654618286414 1105 42-43 0.04669944733743847 1105 44-45 0.010963260382176543 1105 46-47 0.11815096855720242 1105 48-49 0.22121311559257606 1105 50-51 0.221743440534226 1105 52-53 0.19929833509443995 1105 54-55 0.3097860531717913 1105 56-57 0.2161357135404529 1105 58-59 0.31629576349754274 1105 60-61 0.30931071761936124 1105 62-63 0.21342652231913206 1105 64-65 0.12290783187460619 1105 66-67 0.09603405092485318 1105 68-69 0.23882157288359096 1105 70-71 0.10654804827077413 1105 72-73 -0.05714152973888531 1105 74-75 -0.2006572282198853 1105 76-77 -0.15789958341408905 1105 78-79 -0.400652802515161 1105 80-81 -0.16585558217643026 1105 82-83 -0.1907741035316981 1105 84-85 -0.37960240517545785 1105 86-87 -0.33502350902153566 1105 88-89 -0.12644519845378355 1105 90-91 -0.20606243871890584 1105 92-93 -0.49799579315660125 1105 94-95 -0.8161422791243353 1105 96-97 -0.953589736626899 1105 98-99 -0.8886374008543569 1105 100-101 -1.0728945551409819 1106 1 0.32242718941289183 1106 2 0.29695500378048933 1106 3 0.408187098295123 1106 4 0.32334246763038976 1106 5 0.3894226841468864 1106 6 0.25171998922922256 1106 7 0.31160755870147483 1106 8 0.17507928153969488 1106 9 0.42942193456818956 1106 10-11 0.3164711284224566 1106 12-13 0.43700055263074233 1106 14-15 0.4079265533937928 1106 16-17 0.030342442497996558 1106 18-19 0.3403845242159491 1106 20-21 0.5052647803915207 1106 22-23 0.5036083010432009 1106 24-25 0.43190909446649073 1106 26-27 0.3604347943512991 1106 28-29 0.29370272894499294 1106 30-31 0.23152060081684311 1106 32-33 0.39101966878327943 1106 34-35 0.23168537831758584 1106 36-37 0.2615260477735646 1106 38-39 0.20099850786766638 1106 40-41 0.07643910485036542 1106 42-43 0.05285225211388678 1106 44-45 0.2742672285259502 1106 46-47 0.20061094991158512 1106 48-49 0.33704949040909327 1106 50-51 0.4119299462667598 1106 52-53 0.44527068404512704 1106 54-55 0.4050500175467704 1106 56-57 0.16660111005729306 1106 58-59 0.2339101662428078 1106 60-61 0.5965460341980915 1106 62-63 0.3479589686633986 1106 64-65 -0.022844836035098126 1106 66-67 -0.017563362492055035 1106 68-69 -0.015871319963633113 1106 70-71 -0.04701197473870522 1106 72-73 -0.12469828480692158 1106 74-75 0.022030913962840515 1106 76-77 -0.4118358659943233 1106 78-79 -0.42434215467923764 1106 80-81 -0.32190871225934714 1106 82-83 -0.1739682559496778 1106 84-85 -0.34585706657903614 1106 86-87 -0.5272580569645697 1106 88-89 -0.8365148219710576 1106 90-91 -0.6727255928030864 1106 92-93 -0.6742357760978486 1106 94-95 -0.7064197814054474 1106 96-97 -0.5338100127406769 1106 98-99 -0.7440128917894135 1106 100-101 -1.0541457948759767 1107 1 -0.05233971517185054 1107 2 -0.013444977672492087 1107 3 -0.020909683489954034 1107 4 0.059178380706086386 1107 5 0.005673595641269458 1107 6 -0.12438391959990724 1107 7 0.0528483546312728 1107 8 -0.07430623892086885 1107 9 -0.09149420969791322 1107 10-11 -0.06563967605034549 1107 12-13 -0.046802733429551324 1107 14-15 -0.02570790662752387 1107 16-17 -0.03240402186612812 1107 18-19 -0.020389219146608184 1107 20-21 0.042497494367971456 1107 22-23 0.024902494031117328 1107 24-25 -0.04493676095157895 1107 26-27 -0.01885393163872351 1107 28-29 -0.0408234755070751 1107 30-31 -0.09136734584820516 1107 32-33 -0.036212130964848654 1107 34-35 -0.051889110075428846 1107 36-37 0.01369952719095835 1107 38-39 -0.13719423333441938 1107 40-41 -0.04653543104753055 1107 42-43 -0.091514570409025 1107 44-45 -0.09147178072175421 1107 46-47 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>>Per sequence quality scores pass #Quality Count 2 6852.0 3 2118.0 4 2033.0 5 2369.0 6 2631.0 7 3282.0 8 4083.0 9 5226.0 10 6677.0 11 8305.0 12 10511.0 13 12791.0 14 14890.0 15 18194.0 16 21314.0 17 26391.0 18 25940.0 19 23209.0 20 23313.0 21 24958.0 22 27335.0 23 30372.0 24 33891.0 25 37533.0 26 42009.0 27 48469.0 28 55328.0 29 64348.0 30 74170.0 31 89987.0 32 108779.0 33 135421.0 34 175358.0 35 232568.0 36 295264.0 37 324181.0 38 168986.0 39 10813.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86114257204351 18.21390722929739 14.205354509069853 23.719595689589244 2 15.780346584208557 19.304311995826765 40.03455563625713 24.880785783707548 3 19.327563145947924 22.998910122927573 29.626628328245115 28.04689840287939 4 12.91889374954199 15.984655488125435 36.05641675242728 35.0400340099053 5 13.702777801815127 37.038389876014335 34.39366895649002 14.865163365680523 6 33.542453105187214 35.18172195420559 16.691565492413755 14.584259448193437 7 29.250711669559482 32.533042776667806 19.926565536434023 18.289680017338693 8 26.946337957371508 34.93498370327913 19.161031617685527 18.957646721663835 9 26.49918940593149 16.361074371664436 19.887454406928235 37.25228181547584 10-11 25.458381463567132 26.113210500653782 26.717916596609072 21.71049143917002 12-13 26.131053160875716 23.485824874269255 27.30750687780296 23.075615087052068 14-15 22.8494766212156 25.01452660342862 25.495514790278634 26.640481985077148 16-17 22.388248229389305 28.14512555467073 26.597248000203923 22.86937821573605 18-19 22.43172550876868 26.461274044484846 28.984674752406185 22.122325694340294 20-21 22.71718728769912 25.685422466949625 29.533392674215598 22.06399757113566 22-23 22.59430214443285 25.605940566551837 29.35832799504285 22.441429293972465 24-25 22.414377438509142 25.915808941598772 29.361346911164816 22.308466708727273 26-27 22.036694173791556 26.27868980075506 29.11139862053142 22.573217404921966 28-29 22.170929967145394 26.704601442489423 28.997664881210707 22.12680370915448 30-31 22.234565645843034 26.218478294748067 29.02571105058966 22.52124500881924 32-33 21.951656336114013 26.094004274224915 29.081819934109305 22.87251945555177 34-35 22.736284758598238 26.263735488742363 28.810714437650592 22.189265315008807 36-37 22.710779363796448 25.94480005950888 28.819765681532434 22.52465489516224 38-39 22.321005240002485 26.188847024354878 28.855442741892755 22.63470499374988 40-41 22.676387926764423 26.057481548057375 28.90174383195462 22.364386693223576 42-43 22.299034188767006 26.076789577689848 28.701698351318583 22.922477882224566 44-45 22.439329411791455 26.388437287542093 28.819512816256736 22.352720484409712 46-47 22.80664451404157 26.579397876731832 28.202799308314148 22.411158300912454 48-49 22.60326583254809 26.66568283605168 28.101749611621873 22.62930171977836 50-51 22.78540427510879 26.904736185604172 27.98089288577234 22.3289666535147 52-53 22.864662170365058 26.566290287451988 28.01032515508421 22.55872238709874 54-55 22.408780387302876 26.80927549388428 28.13059061448748 22.65135350432536 56-57 22.609724834799483 27.03175150968696 27.854196136310833 22.504327519202725 58-59 22.592660982738092 26.62003789487855 27.930553951082317 22.85674717130104 60-61 22.429201476371844 26.849062965899428 28.08983194708417 22.631903610644557 62-63 22.30795207628135 26.843809019182906 28.386286912394926 22.46195199214082 64-65 22.254120567103357 26.811988815822836 28.126222857258494 22.807667759815317 66-67 22.1918016285237 26.877325930525693 28.0536865750138 22.877185865936813 68-69 22.576792658854483 26.547537792766434 28.202647967036075 22.673021581343004 70-71 22.397454101881404 26.709212006873233 27.970131088101287 22.923202803144076 72-73 22.347198038509845 26.776037055827288 27.92915385722664 22.947611048436226 74-75 22.463538753291402 27.16957492575867 27.546761804712332 22.82012451623759 76-77 22.680799773758743 26.834118236518506 27.641489445863503 22.843592543859252 78-79 22.608287830010394 26.88396715167423 27.709040018750724 22.798704999564652 80-81 22.800381483717032 26.964601357472752 27.43324887492995 22.801768283880268 82-83 22.747804223211638 27.067042376474415 27.43796298296007 22.74719041735388 84-85 22.66107133479809 27.19647275856151 27.37192551328115 22.770530393359255 86-87 22.92489920890299 27.031838028582065 27.2259407434284 22.81732201908654 88-89 22.706556548964024 27.109050959193482 27.158722646172702 23.025669845669796 90-91 22.79492623416894 27.01356861704681 27.16565326202678 23.025851886757465 92-93 22.96074857853605 26.66658629371189 27.32065533377675 23.0520097939753 94-95 22.76514135709085 26.625487698056077 27.402574686352686 23.206796258500383 96-97 23.09870488517322 26.235315015421605 27.25941166128732 23.406568438117855 98-99 22.79057487204106 26.513374429679605 26.963922708183574 23.732127990095762 100-101 23.768041797846294 25.335916189473984 27.13978874409379 23.756253268585937 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1095.0 1 736.5 2 536.5 3 718.0 4 608.0 5 564.0 6 1032.0 7 2222.0 8 3500.0 9 3927.5 10 3164.0 11 2307.0 12 2211.5 13 2334.5 14 2459.0 15 2547.5 16 2660.5 17 2858.5 18 3162.5 19 3531.0 20 3998.0 21 4765.0 22 5786.5 23 7093.0 24 8801.5 25 11123.5 26 14000.0 27 16940.5 28 19164.5 29 21365.0 30 24481.0 31 28615.5 32 34537.5 33 37310.0 34 37874.0 35 40425.5 36 44384.5 37 48840.0 38 52077.0 39 54425.0 40 57209.0 41 61353.5 42 64064.0 43 66029.5 44 68818.5 45 72718.5 46 75481.5 47 77854.0 48 79413.5 49 79572.5 50 80648.0 51 80777.0 52 82220.0 53 84571.5 54 85522.0 55 80629.5 56 72819.0 57 66549.5 58 60839.0 59 52885.0 60 42479.0 61 35158.5 62 29062.5 63 21941.5 64 15842.5 65 11785.5 66 8839.5 67 6775.5 68 5282.0 69 4058.0 70 3169.0 71 2316.5 72 1605.5 73 1190.5 74 895.5 75 656.0 76 457.0 77 276.0 78 171.5 79 109.5 80 58.0 81 42.0 82 29.0 83 20.0 84 13.5 85 8.5 86 8.0 87 7.5 88 5.0 89 4.5 90 5.0 91 5.5 92 3.5 93 2.5 94 2.0 95 1.5 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14418837219873631 2 0.05122960134551571 3 0.15100686394836127 4 0.13150597754443386 5 0.1928724032910587 6 0.19764534751579615 7 0.16618937224419292 8 0.07295786172098732 9 0.12482385562980136 10-11 0.10470930496840765 12-13 0.15187053956998045 14-15 0.13948361289149508 16-17 0.13584708395836173 18-19 0.13202872857857176 20-21 0.13584708395836173 22-23 0.19475885267512158 24-25 0.21160052729669532 26-27 0.14805218419019045 28-29 0.1270739579071776 30-31 0.09766353016046185 32-33 0.12621028228555844 34-35 0.09457248056729851 36-37 0.08675394336106186 38-39 0.05188872221464612 40-41 0.06141188235828901 42-43 0.06298013546070276 44-45 0.04250193190599573 46-47 0.055184326560298196 48-49 0.046002091004136554 50-51 0.05647983999272694 52-53 0.027773989726805764 54-55 0.053706986681212786 56-57 0.02741033683349243 58-59 0.053911541433701526 60-61 0.03911541433701532 62-63 0.05443429246783945 64-65 0.04093367880358198 66-67 0.04157007136688031 68-69 0.06802581935542525 70-71 0.0557298059002682 72-73 0.05288876767125778 74-75 0.045002045547524884 76-77 0.02172826037547161 78-79 0.023569253147870357 80-81 0.02693304241101868 82-83 0.02306923041956453 84-85 0.020341833719714535 86-87 0.024955679803627437 88-89 0.06657120778217192 90-91 0.11089140415473432 92-93 0.08245829355879812 94-95 0.07684440201827356 96-97 0.06925314787035775 98-99 0.056616209827719435 100-101 0.12950588663121052 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2199900.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.780545720465945 #Duplication Level Percentage of deduplicated Percentage of total 1 77.6798496195309 43.330244032611624 2 12.939422780799203 14.435361280416172 3 4.082890631941478 6.832376027000212 4 1.7782735439856499 3.9677227489514624 5 0.934490196611735 2.6063186568714047 6 0.5334967342144533 1.7855243384741153 7 0.33900263622200727 1.3236826434398112 8 0.2561786010558549 1.1431825735040895 9 0.19205320246008126 0.9641549196527819 >10 1.1234548710776235 11.899563084214588 >50 0.09381465656580483 3.6006181835585895 >100 0.0418453405715508 4.240362541770814 >500 0.0031853158372829146 1.2719969402624334 >1k 0.0017968448312877907 1.5695859092378657 >5k 2.450242951756078E-4 1.0293061200339924 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9227 0.41942815582526477 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8244 0.37474430655938906 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5122 0.2328287649438611 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2821 0.12823310150461384 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2535 0.11523251056866221 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2318 0.10536842583753805 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2302 0.1046411200509114 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3636983499249966E-4 2 0.0 0.0 0.0 0.0 1.3636983499249966E-4 3 0.0 0.0 0.0 0.0 1.3636983499249966E-4 4 0.0 0.0 0.0 0.0 1.3636983499249966E-4 5 0.0 0.0 0.0 0.0 1.3636983499249966E-4 6 0.0 0.0 0.0 0.0 1.3636983499249966E-4 7 0.0 0.0 0.0 0.0 1.3636983499249966E-4 8 0.0 0.0 0.0 0.0 1.3636983499249966E-4 9 0.0 0.0 0.0 0.0 1.3636983499249966E-4 10-11 4.5456611664166555E-5 0.0 0.0 0.0 1.3636983499249966E-4 12-13 1.3636983499249966E-4 0.0 0.0 4.5456611664166555E-5 4.3183781080958225E-4 14-15 1.818264466566662E-4 0.0 0.0 4.5456611664166555E-5 4.545661166416655E-4 16-17 2.2728305832083275E-4 0.0 0.0 1.3636983499249966E-4 4.545661166416655E-4 18-19 2.2728305832083275E-4 0.0 0.0 1.3636983499249966E-4 4.545661166416655E-4 20-21 2.2728305832083275E-4 0.0 0.0 2.045547524887495E-4 5.454793399699986E-4 22-23 2.727396699849993E-4 0.0 0.0 2.954679758170826E-4 5.909359516341651E-4 24-25 2.727396699849993E-4 0.0 0.0 3.4092458748124913E-4 5.909359516341651E-4 26-27 2.727396699849993E-4 0.0 0.0 6.818491749624983E-4 5.909359516341651E-4 28-29 2.727396699849993E-4 0.0 0.0 0.0034319741806445748 5.909359516341651E-4 30-31 2.727396699849993E-4 0.0 0.0 0.01206873039683622 6.136642574662485E-4 32-33 2.727396699849993E-4 0.0 0.0 0.02931951452338743 6.363925632983318E-4 34-35 3.636528933133324E-4 0.0 0.0 0.056025273876085274 6.363925632983318E-4 36-37 4.545661166416655E-4 0.0 0.0 0.10143642892858767 6.363925632983318E-4 38-39 4.545661166416655E-4 0.0 0.0 0.16978044456566208 6.363925632983318E-4 40-41 4.545661166416655E-4 0.0 0.0 0.24173826083003774 6.363925632983318E-4 42-43 4.545661166416655E-4 0.0 0.0 0.3118096277103505 6.363925632983318E-4 44-45 4.545661166416655E-4 0.0 0.0 0.39444974771580527 7.500340924587481E-4 46-47 4.545661166416655E-4 0.0 0.0 0.49315877994454294 8.864039274512478E-4 48-49 4.545661166416655E-4 0.0 0.0 0.5992545115687076 9.09132233283331E-4 50-51 4.545661166416655E-4 0.0 0.0 0.7101913723351061 9.545888449474976E-4 52-53 5.000227283058321E-4 0.0 0.0 0.8131505977544433 0.0010000454566116642 54-55 5.000227283058321E-4 0.0 0.0 0.9289058593572435 0.0010000454566116642 56-57 5.000227283058321E-4 0.0 0.0 1.0621391881449156 0.0010455020682758308 58-59 5.454793399699986E-4 0.0 0.0 1.2063048320378198 0.0011364152916041638 60-61 5.454793399699986E-4 0.0 0.0 1.359720896404382 0.0011818719032683303 62-63 5.454793399699986E-4 0.0 0.0 1.5069548615846173 0.0012727851265966635 64-65 5.454793399699986E-4 0.0 0.0 1.6564389290422292 0.0014091549615891632 66-67 5.454793399699986E-4 0.0 0.0 1.8242420110005 0.0014091549615891632 68-69 5.909359516341651E-4 0.0 0.0 2.0142506477567164 0.0014091549615891632 70-71 5.909359516341651E-4 0.0 0.0 2.211032319650893 0.0014318832674212465 72-73 5.909359516341651E-4 0.0 0.0 2.4129960452747854 0.0015227964907495795 74-75 5.909359516341651E-4 0.0 0.0 2.6111186872130547 0.0015909814082458293 76-77 5.909359516341651E-4 0.0 0.0 2.8192190554116094 0.0015909814082458293 78-79 6.363925632983318E-4 0.0 0.0 3.0510932315105235 0.0016137097140779127 80-81 6.363925632983318E-4 4.5456611664166555E-5 0.0 3.302945588435838 0.0016818946315741625 82-83 6.363925632983318E-4 4.5456611664166555E-5 0.0 3.5530251375062503 0.001818264466566662 84-85 6.363925632983318E-4 4.5456611664166555E-5 0.0 3.811082321923724 0.0018637210782308288 86-87 6.363925632983318E-4 4.5456611664166555E-5 0.0 4.074117005318424 0.001954634301559162 88-89 6.363925632983318E-4 4.5456611664166555E-5 0.0 4.352811491431428 0.002045547524887495 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4050 0.0 59.370544 1 GTATCAA 7155 0.0 43.122112 1 ATCAACG 9010 0.0 33.887524 3 TATCAAC 9135 0.0 33.42382 2 TCAACGC 9125 0.0 33.356213 4 CAACGCA 9280 0.0 32.850323 5 AACGCAG 9575 0.0 31.882084 6 TGGTATC 1555 0.0 31.501877 2 GTGGTAT 1830 0.0 28.62044 1 CGCAGAG 11110 0.0 27.302202 8 ACGCAGA 11230 0.0 27.096987 7 GCAGAGT 12280 0.0 24.467524 9 TACATGG 6850 0.0 22.703186 2 GAGTACT 8405 0.0 22.59569 12-13 GTACATG 7150 0.0 22.508438 1 CAGAGTA 11870 0.0 22.267712 10-11 GTACTTT 8680 0.0 21.684668 14-15 ACATGGG 7115 0.0 20.988644 3 AGAGTAC 11590 0.0 20.79582 10-11 AGTACTT 8705 0.0 20.287876 12-13 >>END_MODULE