FastQCFastQC Report
Thu 26 May 2016
SRR938164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938164_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199900
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92740.4215646165734806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84180.38265375698895404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52290.2376926223919269No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA28150.12796036183462886No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25710.11686894858857222No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23130.10514114277921723No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22930.1042320105459339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39600.062.2503661
GTATCAA71400.042.5081631
ATCAACG92600.032.5131573
TGGTATC15400.032.0732422
TCAACGC93950.032.0452354
TATCAAC94450.031.9805092
CAACGCA95400.031.5581745
AACGCAG98300.030.7237766
GTGGTAT17500.029.041261
ACGCAGA116500.025.7609657
CGCAGAG115400.025.7184738
GCGCAAT1151.2827531E-424.7812441
GCAGAGT126550.023.3774389
TAAGGTG11550.023.02385
GTACATG71550.022.835921
TACATGG69100.022.4750122
CGCACCG1100.002882912321.5848145
CAGAGTA123250.021.55777410-11
GAGTACT88850.021.46043812-13
GTAAGGT11300.021.4320014
Produced by FastQC (version 0.11.5)