##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938164_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2199900 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.533907904904765 34.0 31.0 34.0 31.0 34.0 2 32.749970907768535 34.0 31.0 34.0 31.0 34.0 3 32.79895267966726 34.0 31.0 34.0 31.0 34.0 4 36.253573798809036 37.0 35.0 37.0 35.0 37.0 5 36.225609800445476 37.0 37.0 37.0 35.0 37.0 6 36.2563180144552 37.0 37.0 37.0 35.0 37.0 7 36.21512386926678 37.0 37.0 37.0 35.0 37.0 8 36.200920950952316 37.0 37.0 37.0 35.0 37.0 9 37.897409882267375 39.0 38.0 39.0 35.0 39.0 10-11 37.87595254329742 39.0 38.0 39.0 35.0 39.0 12-13 37.79100186372108 39.0 38.0 39.0 35.0 39.0 14-15 39.08794717941725 40.0 38.5 41.0 36.0 41.0 16-17 38.97015727987636 40.0 38.0 41.0 36.0 41.0 18-19 39.21501886449384 40.5 39.0 41.0 36.0 41.0 20-21 39.194003136506204 40.5 39.0 41.0 36.0 41.0 22-23 39.09789308604937 40.0 39.0 41.0 35.5 41.0 24-25 39.001543024682945 40.0 38.5 41.0 35.0 41.0 26-27 38.832934678849035 40.0 38.0 41.0 35.0 41.0 28-29 38.71742124642029 40.0 38.0 41.0 34.5 41.0 30-31 38.4794590663212 40.0 38.0 41.0 34.0 41.0 32-33 38.27047229419519 40.0 38.0 41.0 34.0 41.0 34-35 38.116700531842355 40.0 38.0 41.0 34.0 41.0 36-37 38.119184281103685 40.0 38.0 41.0 34.0 41.0 38-39 37.97643870175917 40.0 38.0 41.0 33.0 41.0 40-41 37.80114482476476 40.0 37.0 41.0 33.0 41.0 42-43 37.57753102413746 40.0 37.0 41.0 33.0 41.0 44-45 37.316737806263916 40.0 37.0 41.0 32.0 41.0 46-47 36.93819423610164 40.0 36.0 41.0 31.0 41.0 48-49 36.55415177962635 39.0 36.0 41.0 30.5 41.0 50-51 36.181152552388745 39.0 35.0 41.0 29.5 41.0 52-53 35.663746306650296 39.0 35.0 40.0 28.0 41.0 54-55 35.35189235874358 38.0 35.0 40.0 27.5 41.0 56-57 35.005901177326244 38.0 34.0 40.0 27.0 41.0 58-59 34.56814764307468 38.0 34.0 40.0 26.0 41.0 60-61 34.089734760670936 37.0 33.0 40.0 25.0 41.0 62-63 33.61941247329425 36.5 33.0 39.5 23.5 41.0 64-65 33.13019387244875 36.0 32.0 39.0 22.0 40.0 66-67 32.5759739079049 35.5 31.5 39.0 20.5 40.0 68-69 31.983546752125093 35.0 31.0 38.0 18.5 40.0 70-71 31.366167098504476 35.0 30.5 37.5 15.0 39.5 72-73 30.777361243692894 34.5 30.0 37.0 10.0 39.0 74-75 30.17403791081413 34.0 29.0 36.0 7.5 39.0 76-77 28.81736215282513 32.5 28.0 35.0 7.0 37.0 78-79 29.495144324742036 34.0 29.0 35.0 3.5 37.0 80-81 29.168286513023318 34.0 29.0 35.0 2.0 37.0 82-83 28.60807127596709 34.0 28.5 35.0 2.0 36.5 84-85 28.01399699986363 33.0 27.0 35.0 2.0 36.0 86-87 27.37671485067503 33.0 26.0 35.0 2.0 35.5 88-89 26.630293649711348 32.0 25.0 35.0 2.0 35.0 90-91 26.082577844447474 32.0 24.0 34.0 2.0 35.0 92-93 25.265749124960223 31.5 20.5 34.0 2.0 35.0 94-95 24.4351406882131 31.0 15.5 34.0 2.0 35.0 96-97 23.386403927451248 31.0 2.0 34.0 2.0 35.0 98-99 21.94724555661621 30.0 2.0 34.0 2.0 35.0 100-101 17.577030546843037 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1071581732025706 1101 2 0.1549607043023542 1101 3 0.10514967546028942 1101 4 0.09773090653479244 1101 5 0.07200396676569909 1101 6 -0.012235028902438216 1101 7 0.07626077455712021 1101 8 0.09494979778290968 1101 9 0.052520055289051015 1101 10-11 0.06467396244758561 1101 12-13 0.20110387026102927 1101 14-15 0.2087272030885714 1101 16-17 0.257819481420249 1101 18-19 0.21398498835497293 1101 20-21 0.25533728745639195 1101 22-23 0.25128712207810366 1101 24-25 0.23954467261167878 1101 26-27 0.15090685241322888 1101 28-29 0.17832417990047134 1101 30-31 0.30731337247287627 1101 32-33 0.3134341006351704 1101 34-35 0.30682881294632836 1101 36-37 0.3934847907596648 1101 38-39 0.37000899035027146 1101 40-41 0.3469177770948946 1101 42-43 0.41255873124147513 1101 44-45 0.4682991387915507 1101 46-47 0.4309750935882093 1101 48-49 0.44162752640622216 1101 50-51 0.3530289193673397 1101 52-53 0.13267787713542845 1101 54-55 0.18285464012181762 1101 56-57 0.2337446961940941 1101 58-59 0.3547578479947262 1101 60-61 0.30378674707282727 1101 62-63 0.3577954713381004 1101 64-65 0.3887624648179937 1101 66-67 0.16780635048970538 1101 68-69 0.13579409757827676 1101 70-71 0.3158591554928414 1101 72-73 0.11104850206580963 1101 74-75 -0.21701861434636172 1101 76-77 -0.1750170724173543 1101 78-79 -0.12534803904531344 1101 80-81 -0.10150598043184189 1101 82-83 -0.05252296142532131 1101 84-85 -0.18843403056181174 1101 86-87 -0.22433129031389498 1101 88-89 -0.30537948813346816 1101 90-91 -0.4321996004104207 1101 92-93 -0.602493961073808 1101 94-95 -0.677337288424237 1101 96-97 -0.6741685299475471 1101 98-99 -0.693358276951912 1101 100-101 -1.1339357597297237 1102 1 0.13059181608490178 1102 2 0.13112151304809316 1102 3 0.053650909737946506 1102 4 0.07770915973804193 1102 5 0.05506203659054876 1102 6 0.080632609512989 1102 7 0.030760010480477717 1102 8 0.054236267748819955 1102 9 0.1752748454618498 1102 10-11 0.153533137244807 1102 12-13 0.16114560060075434 1102 14-15 0.11178204028146865 1102 16-17 0.1874832876974324 1102 18-19 0.2036023622823251 1102 20-21 0.15906656922434337 1102 22-23 0.20766569266703527 1102 24-25 0.2631502327386386 1102 26-27 0.2043099322298474 1102 28-29 0.17691651562399358 1102 30-31 0.17601855643901843 1102 32-33 0.21735734032013454 1102 34-35 0.15323618334718248 1102 36-37 0.2481500664150076 1102 38-39 0.23269560137647716 1102 40-41 0.22301540257978303 1102 42-43 0.35600677402976544 1102 44-45 0.15393155915125334 1102 46-47 0.20477314225400534 1102 48-49 0.209947733294662 1102 50-51 0.17057035583142266 1102 52-53 0.37410405557671567 1102 54-55 0.3834982277555241 1102 56-57 0.39474005295910786 1102 58-59 0.4313344907595038 1102 60-61 0.39400509666727856 1102 62-63 0.4289501086955667 1102 64-65 0.4753632053845891 1102 66-67 0.2624592833685071 1102 68-69 0.10838872553173218 1102 70-71 0.09105017465352816 1102 72-73 0.2741465389765878 1102 74-75 0.2417903077178245 1102 76-77 0.19274477077060936 1102 78-79 0.03232211494551862 1102 80-81 0.08057936105164387 1102 82-83 0.2145997022634063 1102 84-85 -0.022832173267818945 1102 86-87 0.09875264056655553 1102 88-89 0.2209929110671922 1102 90-91 0.22756441745722 1102 92-93 -0.030867653327245392 1102 94-95 -0.48006886242211877 1102 96-97 -0.4866876318636173 1102 98-99 -0.4548914261231829 1102 100-101 -0.9677623143318144 1103 1 0.10996527805053802 1103 2 0.07495086473257828 1103 3 0.07375805492839049 1103 4 0.02928312694115931 1103 5 -0.014459838885834131 1103 6 0.026726274444712317 1103 7 -0.030859907200657233 1103 8 0.013929423087574833 1103 9 0.1024994148122218 1103 10-11 0.14977380015123032 1103 12-13 0.1359517810489308 1103 14-15 0.05069975408186167 1103 16-17 0.09150558783463225 1103 18-19 0.13602194894580322 1103 20-21 0.1650798752632383 1103 22-23 0.203179260035661 1103 24-25 0.13959217398299728 1103 26-27 0.21039129288666203 1103 28-29 0.2686437962703465 1103 30-31 0.2710059255019317 1103 32-33 0.22656540234239486 1103 34-35 0.2575189965617497 1103 36-37 0.1577411398268893 1103 38-39 0.1838013528083451 1103 40-41 0.15750521072020973 1103 42-43 0.15757448020803366 1103 44-45 0.07713382836443117 1103 46-47 0.1649976680494305 1103 48-49 0.06045079303029155 1103 50-51 -0.0640509338308064 1103 52-53 -0.046786262156686576 1103 54-55 -0.027481432080271873 1103 56-57 -0.007235980926651564 1103 58-59 0.0338544171516304 1103 60-61 -0.06756936052908458 1103 62-63 -0.07958584592017104 1103 64-65 -0.2851185668665508 1103 66-67 -0.492352722554962 1103 68-69 -0.38845054579386584 1103 70-71 -0.24777797926968148 1103 72-73 -0.6426150411971925 1103 74-75 -0.7436544428211711 1103 76-77 -0.7311255225720146 1103 78-79 -0.5821000196828479 1103 80-81 -0.5866782011775022 1103 82-83 -0.5887238130570474 1103 84-85 -0.7519394055681765 1103 86-87 -0.9719092858691205 1103 88-89 -1.1286172720813958 1103 90-91 -1.1368471006507548 1103 92-93 -1.329642125767844 1103 94-95 -1.2227203247797505 1103 96-97 -1.190005430596166 1103 98-99 -1.611403708620621 1103 100-101 -2.134378257623265 1104 1 0.08173343579728964 1104 2 0.12672112620089848 1104 3 0.0690049590189119 1104 4 0.1039622082621321 1104 5 0.07358444849871404 1104 6 0.05729142710612223 1104 7 0.1746524501407265 1104 8 0.14788692791196922 1104 9 0.15649173218289292 1104 10-11 0.11647254520040917 1104 12-13 0.1818703083629245 1104 14-15 0.2158702162128492 1104 16-17 0.11641167891768589 1104 18-19 0.1542719863677675 1104 20-21 0.15461992890814003 1104 22-23 0.16086664296557984 1104 24-25 0.16917474707721425 1104 26-27 0.2245878735299982 1104 28-29 0.025576216237901406 1104 30-31 -0.1404529346046317 1104 32-33 0.04176130129902589 1104 34-35 -0.011158106451823357 1104 36-37 -0.015048991696168912 1104 38-39 0.10790479320223056 1104 40-41 0.16359336069937314 1104 42-43 0.16475393574871333 1104 44-45 0.059777366905017004 1104 46-47 0.061419975993814546 1104 48-49 0.022715793171883547 1104 50-51 -0.024439525591496647 1104 52-53 0.07769185307061832 1104 54-55 -0.0591348560894005 1104 56-57 0.14693393909147545 1104 58-59 0.025913373948469598 1104 60-61 -0.1823888146942565 1104 62-63 -0.02307032692801414 1104 64-65 -0.020552823544853993 1104 66-67 -0.15531781695946734 1104 68-69 -0.11464865568095206 1104 70-71 -0.25009333724622707 1104 72-73 -0.7728617025506175 1104 74-75 -0.7614779203538298 1104 76-77 -0.7250386368485984 1104 78-79 -0.6159969919420476 1104 80-81 -0.47216115698720884 1104 82-83 -0.6963203212838138 1104 84-85 -0.712523956969779 1104 86-87 -0.5021818897504886 1104 88-89 -0.6542068198217166 1104 90-91 -0.8383383661606416 1104 92-93 -0.9247222492401193 1104 94-95 -0.9161286975915779 1104 96-97 -1.0906612253376728 1104 98-99 -1.2810771968741683 1104 100-101 -1.694933073729679 1105 1 0.10705458204235185 1105 2 0.08235923808901191 1105 3 0.09264436309310042 1105 4 0.023259487070284024 1105 5 0.05552234755618457 1105 6 0.024300776452427897 1105 7 0.05891058331190635 1105 8 0.0160611449992345 1105 9 -0.0033155800700654936 1105 10-11 0.054474217441210726 1105 12-13 0.05599523235198234 1105 14-15 0.07033870967590872 1105 16-17 0.050658410447020685 1105 18-19 0.10289143756873642 1105 20-21 0.09921079999357829 1105 22-23 0.14079785305982995 1105 24-25 0.10669565008723936 1105 26-27 0.15294945759809764 1105 28-29 0.182997734936599 1105 30-31 0.1094064451460568 1105 32-33 0.11883544997643725 1105 34-35 0.20687263997565708 1105 36-37 0.2322079633053491 1105 38-39 0.25403868648286476 1105 40-41 0.14744768572217737 1105 42-43 0.17072649579736066 1105 44-45 0.17538716471590732 1105 46-47 0.1279523566566283 1105 48-49 0.3309430278936887 1105 50-51 0.16239727890835098 1105 52-53 0.2949919442837583 1105 54-55 0.3491436860206676 1105 56-57 0.34827933282817014 1105 58-59 0.35417614378732765 1105 60-61 0.24468533398314918 1105 62-63 0.3431532592683979 1105 64-65 0.2559227389361425 1105 66-67 0.12028456978422497 1105 68-69 0.02545091865775362 1105 70-71 -0.07952572551013404 1105 72-73 0.020310614263006954 1105 74-75 0.005175127685088654 1105 76-77 -0.02363718503953649 1105 78-79 -0.18700071647838 1105 80-81 -0.17821257839189286 1105 82-83 -0.19107746631269862 1105 84-85 -0.16525750060006317 1105 86-87 -0.14536560820594602 1105 88-89 -0.217284503008095 1105 90-91 -0.4326033403169234 1105 92-93 -0.28877477280351016 1105 94-95 -0.44585752854323246 1105 96-97 -0.5374906187703274 1105 98-99 -0.7484010415077975 1105 100-101 -1.514673116295807 1106 1 0.1790149997834547 1106 2 0.1273950912250399 1106 3 0.11007503711221744 1106 4 0.157425343776751 1106 5 0.19770383484146237 1106 6 0.12344064831341939 1106 7 0.11148625673595092 1106 8 0.17555949863115927 1106 9 0.10475236732549575 1106 10-11 0.1196664259547191 1106 12-13 0.106690847657255 1106 14-15 0.09396892648076971 1106 16-17 0.14426732860467695 1106 18-19 0.28072782726207635 1106 20-21 0.2948968941340908 1106 22-23 0.29593407514099823 1106 24-25 0.3162249970940323 1106 26-27 0.2566153539760876 1106 28-29 0.22161684371589985 1106 30-31 0.22162031430938356 1106 32-33 0.3096211196316929 1106 34-35 0.29293516724982993 1106 36-37 0.1704568872153729 1106 38-39 0.3050105147448505 1106 40-41 0.30883626329983116 1106 42-43 0.24089822388876314 1106 44-45 0.19346361669352774 1106 46-47 0.052510515104678746 1106 48-49 0.050932886861431825 1106 50-51 0.030768911788108255 1106 52-53 0.03814959890350167 1106 54-55 -0.2588341326408141 1106 56-57 -0.20555690313802444 1106 58-59 -0.008648418834162896 1106 60-61 -0.0709885115756137 1106 62-63 0.014086804513773643 1106 64-65 -0.17999969828368023 1106 66-67 -0.46984391635057676 1106 68-69 -0.46181402758733725 1106 70-71 -0.8676645609526723 1106 72-73 -0.6968455824283915 1106 74-75 -0.5555521752466355 1106 76-77 -0.7001249910061347 1106 78-79 -0.49183543500894444 1106 80-81 -0.5073975518119731 1106 82-83 -0.4586189726403056 1106 84-85 -0.6252662531291229 1106 86-87 -0.5960596118557255 1106 88-89 -0.5402845625153745 1106 90-91 -0.9253811401344336 1106 92-93 -0.8046540424508315 1106 94-95 -1.071045100447865 1106 96-97 -1.3679770659873682 1106 98-99 -1.2948282492278729 1106 100-101 -1.8754012870420294 1107 1 -0.03265597775408935 1107 2 -0.01061598537285846 1107 3 0.04432277374097282 1107 4 -0.05906786009484932 1107 5 -0.05058329934144723 1107 6 -0.01864508705641299 1107 7 -0.05971830688186941 1107 8 -0.04611834218025734 1107 9 0.03467742247445926 1107 10-11 -0.01339294508244393 1107 12-13 -0.03650431909318286 1107 14-15 -0.07424132066270772 1107 16-17 -0.14774310322166428 1107 18-19 -0.060082603339459695 1107 20-21 -0.1374771118613225 1107 22-23 -0.04010716749213117 1107 24-25 0.00405692430920368 1107 26-27 0.015992208951921327 1107 28-29 -0.14483244220178193 1107 30-31 -0.1631468921019419 1107 32-33 -0.06337506904228718 1107 34-35 -0.033503615977885204 1107 36-37 -0.1893314361204972 1107 38-39 -0.2695326663585291 1107 40-41 -0.3009931784391071 1107 42-43 -0.316970460554181 1107 44-45 -0.34622318792975904 1107 46-47 -0.38029534348038396 1107 48-49 -0.4505922122054855 1107 50-51 -0.5854304923449973 1107 52-53 -0.4700126228200503 1107 54-55 -0.43040690280950855 1107 56-57 -0.49419147032965327 1107 58-59 -0.5325902651641954 1107 60-61 -0.5868254394885 1107 62-63 -0.4860470490111055 1107 64-65 -0.5893452114129119 1107 66-67 -0.7543235563983295 1107 68-69 -0.7323938976794295 1107 70-71 -0.702347583995163 1107 72-73 -0.9123967811829381 1107 74-75 -0.8453095154283723 1107 76-77 -0.7789613270764661 1107 78-79 -0.7674775710487438 1107 80-81 -0.8109776743010713 1107 82-83 -0.6672906032605681 1107 84-85 -0.8882180033077027 1107 86-87 -0.9258277827998 1107 88-89 -1.0884832046457262 1107 90-91 -1.3625929012225129 1107 92-93 -1.2520923659398058 1107 94-95 -1.2792709900816952 1107 96-97 -1.5554945905195297 1107 98-99 -1.653693376928949 1107 100-101 -2.0990422035274925 1108 1 -0.049621312335844436 1108 2 -0.004822160154994037 1108 3 0.0024366576809669027 1108 4 0.04914977012623467 1108 5 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66.0 7 161.0 8 453.0 9 938.0 10 1649.0 11 2715.0 12 4286.0 13 6405.0 14 8496.0 15 11949.0 16 15502.0 17 21403.0 18 22237.0 19 18435.0 20 17161.0 21 18035.0 22 19414.0 23 21163.0 24 23667.0 25 27203.0 26 30869.0 27 36867.0 28 43964.0 29 52537.0 30 63604.0 31 76989.0 32 96072.0 33 124044.0 34 167593.0 35 238832.0 36 341168.0 37 433320.0 38 239787.0 39 12859.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08178316475785 18.583065835586286 14.56280313696324 23.772347862692623 2 16.00371524966323 19.587576372430593 39.531674867463515 24.87703351044266 3 19.33501931851709 22.971692087522726 29.9250853410665 27.768203252893684 4 13.112550570480478 16.296922587390338 35.78699031774172 34.803536524387475 5 13.673506495867919 37.08004145718364 34.47733037556935 14.769121671379088 6 33.614578375633734 35.42191983948077 16.437584010989788 14.525917773895713 7 29.222419200872768 32.53075139779081 19.836628937678984 18.41020046365744 8 26.927678530842307 35.149597708986775 19.002091004136552 18.920632756034365 9 26.34074276103459 16.3157416246193 19.777626255738898 37.56588935860721 10-11 25.461884631119595 26.219555434337927 26.584526569389517 21.73403336515296 12-13 26.027838753242786 23.49243118838593 27.308565955553092 23.171164102818196 14-15 22.76803491067776 25.009773171507792 25.45911177780808 26.763080140006362 16-17 22.327974694563498 28.20100611702605 26.630303473860756 22.840715714549695 18-19 22.410950497749898 26.489044956588938 28.92529205872994 22.174712486931224 20-21 22.682690122278284 25.63357425337515 29.607095777080776 22.076639847265785 22-23 22.511409609527703 25.58377653529706 29.38042638301741 22.524387472157827 24-25 22.381563578284492 25.91448980245879 29.392093305837136 22.311853313419576 26-27 22.018536531916766 26.26548963864197 29.124782989909725 22.59119083953154 28-29 22.18636230667524 26.659979584168937 29.05887382893661 22.094784280219216 30-31 22.209781881120495 26.175561093856007 29.042165710343852 22.57249131467964 32-33 21.952066003000137 25.999931815082505 29.174553388790397 22.87344879312696 34-35 22.617515216248364 26.34037890089328 28.83269093644169 22.20941494641667 36-37 22.67494770183546 25.874727144952058 28.87604175852749 22.574283394684997 38-39 22.270382170020575 26.195657831424978 28.934122873629082 22.599837124925365 40-41 22.599435963594015 26.052886140832726 28.94525464825457 22.40242324731869 42-43 22.261950003454807 26.02946497732554 28.788743867713535 22.919841151506116 44-45 22.40956407109414 26.34335651620528 28.893199690895038 22.353879721805537 46-47 22.798966133884388 26.59300567571769 28.187715975922522 22.4203122144754 48-49 22.555170990523155 26.59844501174605 28.18048009716858 22.66590390056221 50-51 22.698942653156642 26.87384776378382 28.017020649845886 22.410188933213657 52-53 22.879686211470847 26.568965376156186 27.99086510966505 22.56048330270792 54-55 22.417398977276832 26.751413989672756 28.119568189613613 22.7116188434368 56-57 22.593219079071947 26.965443886466506 27.84352602250638 22.597811011955166 58-59 22.542925389719823 26.607637601429683 27.90739705510923 22.942039953741265 60-61 22.348321783067135 26.85958528142267 28.050486293103255 22.741606642406943 62-63 22.302585783984988 26.871730146959532 28.301815591086676 22.5238684779688 64-65 22.156924793457193 26.85861246335971 28.06867983102841 22.91578291215469 66-67 22.143483230148 26.903003361517218 27.931030250017958 23.022483158316824 68-69 22.528159716514644 26.61024151320721 28.126795400047243 22.7348033702309 70-71 22.356745452168923 26.772719264845453 27.845035604075857 23.02549967890977 72-73 22.297502631518533 26.814827240325208 27.7669016679202 23.120768460236054 74-75 22.34108790400181 27.227219854683348 27.397641735789023 23.034050505525812 76-77 22.4847182726698 26.84812342047898 27.73814522375122 22.929013083099996 78-79 22.37987704833245 26.891045161923948 27.782343584004053 22.946734205739546 80-81 22.547914702328292 26.9991698634048 27.56826350517676 22.88465192909015 82-83 22.452033687088228 27.103845785692148 27.51364578992111 22.930474737298507 84-85 22.38914174865696 27.13128351345471 27.50797775662551 22.971596981262817 86-87 22.57540279887441 27.063273532191513 27.38434402787734 22.976979641056737 88-89 22.346452790641106 27.271307467246825 27.13349746863356 23.24874227347851 90-91 22.409498201570106 27.134477450455936 27.25503966185289 23.200984686121068 92-93 22.433217610458662 26.77852024006036 27.418137111032355 23.370125038448624 94-95 22.223821013021126 26.751141923068005 27.50217200655216 23.522865057358704 96-97 22.466154126278973 26.474284819304167 27.39414789232333 23.665413162093532 98-99 22.070584737417796 26.703654301994767 27.207983619241936 24.017777341345504 100-101 22.6386230661872 26.052930666872758 27.19819336536844 24.1102529015716 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 949.0 1 662.5 2 556.0 3 737.0 4 578.5 5 498.0 6 999.0 7 2231.5 8 3543.5 9 4059.0 10 3249.0 11 2234.5 12 2112.5 13 2225.0 14 2332.5 15 2471.5 16 2593.5 17 2706.0 18 2973.0 19 3352.5 20 3849.0 21 4629.0 22 5524.0 23 6727.5 24 8463.0 25 10752.5 26 13520.5 27 16526.0 28 18904.0 29 21269.0 30 24476.0 31 28543.0 32 34572.5 33 37339.0 34 37956.0 35 40412.5 36 43993.5 37 48909.0 38 52578.5 39 54776.0 40 57485.0 41 61862.0 42 64562.0 43 65850.0 44 68524.5 45 73105.0 46 76494.5 47 78987.5 48 80004.5 49 79854.0 50 81467.0 51 82002.0 52 83252.0 53 85699.5 54 87258.5 55 81743.0 56 73375.5 57 67174.5 58 60860.5 59 52944.0 60 42300.5 61 34573.0 62 28563.0 63 20879.5 64 14450.0 65 10611.0 66 7920.0 67 6190.0 68 4808.5 69 3687.0 70 2788.0 71 1866.5 72 1292.5 73 997.0 74 748.5 75 540.5 76 369.0 77 215.0 78 113.0 79 73.5 80 40.5 81 20.0 82 14.0 83 10.0 84 5.5 85 1.5 86 2.0 87 2.5 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008773126051184145 2 0.014409745897540798 3 0.00895495249784081 4 0.0 5 0.001818264466566662 6 0.0017728078549024955 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.727623982908315E-4 14-15 0.0 16-17 0.0025682985590254103 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0036592572389654078 26-27 0.018432656029819538 28-29 0.006204827492158734 30-31 0.0030683212873312427 32-33 0.0 34-35 0.008886767580344561 36-37 6.363925632983318E-4 38-39 0.0024773853356970773 40-41 0.001909177689894995 42-43 0.003091049593163326 44-45 0.0 46-47 9.091322332833311E-5 48-49 5.454793399699986E-4 50-51 0.004613846083912905 52-53 0.009182235556161643 54-55 0.00838674485203873 56-57 0.017750806854857037 58-59 0.02416018909950452 60-61 0.0043183781080958225 62-63 0.020500931860539116 64-65 0.021682803763807445 66-67 0.013546070275921633 68-69 0.031978726305741174 70-71 0.016478021728260375 72-73 0.005659348152188735 74-75 0.016296195281603712 76-77 0.020887313059684533 78-79 0.026387563071048684 80-81 0.011909632256011637 82-83 0.03538797218055366 84-85 0.02288740397290786 86-87 0.031569616800763665 88-89 0.022501022773762442 90-91 0.028205827537615347 92-93 0.024387472157825354 94-95 0.014591572344197463 96-97 0.015159779989999544 98-99 0.011182326469384971 100-101 0.010455020682758307 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2199900.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.54772768521101 #Duplication Level Percentage of deduplicated Percentage of total 1 75.87540423600781 40.629554840350515 2 13.889961712374793 14.875517746645054 3 4.422438302300375 7.10434565748682 4 1.9190273869190255 4.110382237408083 5 0.993034934161588 2.658738211819308 6 0.6163805617125856 1.980346708142576 7 0.39761237250452397 1.4903867333000054 8 0.27790194208734714 1.1904814014467637 9 0.2079121864094085 1.0019902626259054 >10 1.241441594596169 12.48935756694017 >50 0.1056698063605707 3.8619533823661443 >100 0.04760756637281563 4.614760952503545 >500 0.0033134625684663344 1.2475945576748244 >1k 0.0020390538882869365 1.701811239431919 >5k 2.5488173603586706E-4 1.0427785018584494 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9274 0.4215646165734806 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8418 0.38265375698895404 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5229 0.2376926223919269 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2815 0.12796036183462886 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2571 0.11686894858857222 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2313 0.10514114277921723 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2293 0.1042320105459339 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 9.091322332833311E-5 7 0.0 0.0 0.0 0.0 9.091322332833311E-5 8 0.0 0.0 0.0 4.5456611664166555E-5 9.091322332833311E-5 9 0.0 0.0 0.0 4.5456611664166555E-5 9.091322332833311E-5 10-11 4.5456611664166555E-5 0.0 0.0 4.5456611664166555E-5 9.091322332833311E-5 12-13 4.5456611664166555E-5 0.0 0.0 6.818491749624983E-5 4.772944224737488E-4 14-15 4.5456611664166555E-5 0.0 0.0 9.091322332833311E-5 5.454793399699986E-4 16-17 6.818491749624983E-5 0.0 0.0 1.3636983499249966E-4 5.454793399699986E-4 18-19 9.091322332833311E-5 0.0 0.0 1.3636983499249966E-4 6.363925632983318E-4 20-21 9.091322332833311E-5 0.0 0.0 2.045547524887495E-4 7.500340924587481E-4 22-23 1.5909814082458294E-4 0.0 0.0 2.954679758170826E-4 7.727623982908314E-4 24-25 2.2728305832083275E-4 0.0 0.0 3.4092458748124913E-4 7.727623982908314E-4 26-27 2.727396699849993E-4 0.0 0.0 6.818491749624983E-4 7.727623982908314E-4 28-29 2.727396699849993E-4 0.0 0.0 0.0033865175689804083 7.954907041229147E-4 30-31 2.727396699849993E-4 0.0 0.0 0.012091458702668305 8.18219009954998E-4 32-33 2.727396699849993E-4 0.0 0.0 0.029478612664212006 8.18219009954998E-4 34-35 3.181962816491659E-4 0.0 0.0 0.05641165507523069 0.0010000454566116642 36-37 3.8638119914541575E-4 0.0 0.0 0.1019364516568935 0.0010227737624437475 38-39 4.09109504977499E-4 0.0 0.0 0.17071230510477747 0.0010909586799399973 40-41 5.000227283058321E-4 0.0 0.0 0.2430110459566344 0.0010909586799399973 42-43 5.000227283058321E-4 0.0 0.0 0.3136733487885813 0.0010909586799399973 44-45 5.000227283058321E-4 0.0 0.0 0.3970862311923269 0.0010909586799399973 46-47 5.000227283058321E-4 0.0 0.0 0.4966816673485158 0.0011136869857720805 48-49 5.000227283058321E-4 0.0 0.0 0.603709259511796 0.0011818719032683303 50-51 5.000227283058321E-4 0.0 0.0 0.7153052411473249 0.0011818719032683303 52-53 5.000227283058321E-4 0.0 0.0 0.8189463157416246 0.0012727851265966635 54-55 5.000227283058321E-4 0.0 0.0 0.9354743397427157 0.00131824173826083 56-57 5.000227283058321E-4 0.0 0.0 1.0693895177053503 0.0013409700440929133 58-59 5.000227283058321E-4 0.0 0.0 1.2146688485840265 0.0013636983499249965 60-61 5.000227283058321E-4 0.0 0.0 1.3688576753488795 0.0013636983499249965 62-63 5.000227283058321E-4 0.0 0.0 1.5168871312332377 0.0014546115732533298 64-65 5.000227283058321E-4 0.0 0.0 1.66703031955998 0.001477339879085413 66-67 5.000227283058321E-4 0.0 0.0 1.8364016546206645 0.0015000681849174963 68-69 5.000227283058321E-4 0.0 0.0 2.0277512614209736 0.0015000681849174963 70-71 5.000227283058321E-4 0.0 0.0 2.2249420428201283 0.0015227964907495795 72-73 5.000227283058321E-4 0.0 0.0 2.4280421837356245 0.0016591663257420793 74-75 5.000227283058321E-4 0.0 0.0 2.6281876448929493 0.001818264466566662 76-77 5.000227283058321E-4 0.0 0.0 2.8382426473930633 0.001954634301559162 78-79 5.000227283058321E-4 0.0 0.0 3.073116959861812 0.001954634301559162 80-81 5.000227283058321E-4 0.0 0.0 3.3279694531569617 0.0020228192190554117 82-83 5.000227283058321E-4 0.0 0.0 3.5820491840538207 0.002227373971544161 84-85 5.000227283058321E-4 0.0 0.0 3.845174780671849 0.002227373971544161 86-87 5.000227283058321E-4 0.0 0.0 4.11291422337379 0.002295558889040411 88-89 5.000227283058321E-4 0.0 0.0 4.398404472930588 0.0023637438065366606 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3960 0.0 62.250366 1 GTATCAA 7140 0.0 42.508163 1 ATCAACG 9260 0.0 32.513157 3 TGGTATC 1540 0.0 32.073242 2 TCAACGC 9395 0.0 32.045235 4 TATCAAC 9445 0.0 31.980509 2 CAACGCA 9540 0.0 31.558174 5 AACGCAG 9830 0.0 30.723776 6 GTGGTAT 1750 0.0 29.04126 1 ACGCAGA 11650 0.0 25.760965 7 CGCAGAG 11540 0.0 25.718473 8 GCGCAAT 115 1.2827531E-4 24.781244 1 GCAGAGT 12655 0.0 23.377438 9 TAAGGTG 1155 0.0 23.0238 5 GTACATG 7155 0.0 22.83592 1 TACATGG 6910 0.0 22.475012 2 CGCACCG 110 0.0028829123 21.584814 5 CAGAGTA 12325 0.0 21.557774 10-11 GAGTACT 8885 0.0 21.460438 12-13 GTAAGGT 1130 0.0 21.432001 4 >>END_MODULE