FastQCFastQC Report
Thu 26 May 2016
SRR938163_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938163_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1870642
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121110.6474247878535818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100430.5368745061855769No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62410.3336287755754441No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25190.13465965160624No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23270.12439579566801132No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20160.1077704873514013No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19840.10605984469502984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49300.056.884241
GTATCAA85150.039.9361721
TGGTATC20950.031.3054562
TATCAAC108400.030.8651472
ATCAACG108600.030.7207813
TCAACGC110150.030.4179254
CAACGCA111200.030.002495
AACGCAG114850.029.0864836
GTGGTAT23500.028.8192081
ACGCAGA128800.025.6362517
CGCAGAG130300.025.1588178
GTGTACG1751.4297984E-724.528221
TACGCTC1401.7729306E-523.7626554
TACATGG83300.023.5059172
GTACATG85150.023.356781
GCAGAGT139400.023.1396989
GAGTACT89200.022.76723912-13
GTATAGG5200.022.0125051
ACATGGG85700.021.7385333
CAGAGTA135900.021.67261510-11
Produced by FastQC (version 0.11.5)