##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938163_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1870642 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.852470969859546 33.0 31.0 34.0 30.0 34.0 2 32.001298484691354 33.0 31.0 34.0 30.0 34.0 3 32.082360494418495 34.0 31.0 34.0 30.0 34.0 4 35.619455245846076 37.0 35.0 37.0 33.0 37.0 5 35.63650126534099 37.0 35.0 37.0 33.0 37.0 6 35.673241058417375 37.0 35.0 37.0 33.0 37.0 7 35.600962129579045 37.0 35.0 37.0 33.0 37.0 8 35.57487108703857 37.0 35.0 37.0 33.0 37.0 9 37.19597068813808 39.0 37.0 39.0 34.0 39.0 10-11 37.092967280751736 39.0 37.0 39.0 33.0 39.0 12-13 36.95020345956094 39.0 37.0 39.0 33.0 39.0 14-15 38.08326366028348 40.0 38.0 41.0 33.0 41.0 16-17 37.90394768213266 40.0 38.0 41.0 33.0 41.0 18-19 38.10222185752271 40.0 38.0 41.0 33.0 41.0 20-21 38.08996643932939 40.0 38.0 41.0 33.0 41.0 22-23 37.97423210854883 40.0 38.0 41.0 33.0 41.0 24-25 37.8285070045471 40.0 38.0 41.0 32.5 41.0 26-27 37.6172600101997 40.0 37.5 41.0 32.0 41.0 28-29 37.39352104785416 40.0 37.0 41.0 32.0 41.0 30-31 37.113160348158544 40.0 36.5 41.0 31.0 41.0 32-33 36.76405560230124 39.0 36.0 41.0 30.5 41.0 34-35 36.45801762175766 39.0 35.0 41.0 30.0 41.0 36-37 36.139692415758866 39.0 35.0 40.5 29.5 41.0 38-39 35.80395179836655 38.5 35.0 40.0 28.5 41.0 40-41 35.609179361951675 38.5 35.0 40.0 27.0 41.0 42-43 35.320981780586564 38.0 35.0 40.0 26.5 41.0 44-45 34.9291363606719 38.0 34.5 40.0 24.5 41.0 46-47 34.435987217222745 38.0 33.5 40.0 23.5 41.0 48-49 33.92081435143656 38.0 33.0 40.0 21.0 41.0 50-51 33.081979876427454 37.5 32.0 39.5 18.0 40.5 52-53 33.06090930279551 37.5 32.5 39.5 17.0 40.5 54-55 33.03386778442909 37.5 32.5 40.0 14.0 41.0 56-57 32.557686077827825 37.0 31.5 40.0 8.5 41.0 58-59 32.08245751993166 36.5 31.0 40.0 4.5 41.0 60-61 31.518144840113713 36.0 30.5 39.0 2.0 41.0 62-63 30.883955347950064 35.0 29.5 39.0 2.0 40.5 64-65 30.271227204350165 35.0 28.5 39.0 2.0 40.0 66-67 29.58102913331359 35.0 28.0 38.0 2.0 40.0 68-69 28.878435317928282 34.0 26.5 37.5 2.0 40.0 70-71 28.05409078808238 34.0 25.5 37.0 2.0 39.0 72-73 27.255695103606143 33.5 23.5 36.0 2.0 39.0 74-75 26.43089885718379 33.0 21.0 35.5 2.0 38.5 76-77 25.590255377565562 32.0 17.0 35.0 2.0 37.5 78-79 24.75409271255537 31.5 9.0 35.0 2.0 37.0 80-81 23.952232174836233 31.0 4.0 35.0 2.0 36.5 82-83 23.11972039545782 30.5 2.0 34.5 2.0 36.0 84-85 22.307361857586862 30.0 2.0 34.0 2.0 35.5 86-87 21.532778586175226 29.0 2.0 34.0 2.0 35.0 88-89 20.70054318250098 29.0 2.0 34.0 2.0 35.0 90-91 19.839698884126413 26.5 2.0 34.0 2.0 35.0 92-93 18.937664181601825 25.5 2.0 34.0 2.0 35.0 94-95 18.005702587667763 24.0 2.0 33.0 2.0 35.0 96-97 16.895002892055242 16.0 2.0 32.5 2.0 35.0 98-99 15.610220982956653 2.0 2.0 32.0 2.0 34.0 100-101 12.630730786542802 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.40756975348853075 1101 2 0.5021502820876975 1101 3 0.4818349217470228 1101 4 0.3120548817923421 1101 5 0.3900100675843916 1101 6 0.26991073741017857 1101 7 0.21715722000077875 1101 8 0.29009461290993244 1101 9 0.4237168654330574 1101 10-11 0.32218522895203705 1101 12-13 0.39409820833295583 1101 14-15 0.5270798127511327 1101 16-17 0.6077104392892494 1101 18-19 0.5806074556448948 1101 20-21 0.5653706971692642 1101 22-23 0.6439664454379326 1101 24-25 0.6601728319328473 1101 26-27 0.6562979445370658 1101 28-29 0.7983997842512949 1101 30-31 0.880917064618906 1101 32-33 1.0550613399919229 1101 34-35 1.1070568650682802 1101 36-37 1.1413732563802697 1101 38-39 1.2771706916091148 1101 40-41 1.3222609761047153 1101 42-43 1.3960517159783876 1101 44-45 1.4823365583947279 1101 46-47 1.7354978948862154 1101 48-49 1.7626991509614385 1101 50-51 1.8571169065272883 1101 52-53 2.0676561784884058 1101 54-55 1.9387737035314814 1101 56-57 2.229541790087268 1101 58-59 2.3510589866717524 1101 60-61 2.4914620160936174 1101 62-63 2.5893394455422047 1101 64-65 2.6929958337640016 1101 66-67 2.8675526356311174 1101 68-69 3.177614889634306 1101 70-71 3.4533469440172837 1101 72-73 3.792727070131985 1101 74-75 4.079857203334786 1101 76-77 4.335657935424237 1101 78-79 4.259848255352519 1101 80-81 4.578464563096734 1101 82-83 4.733311914130635 1101 84-85 5.026669876840067 1101 86-87 5.406539846673489 1101 88-89 5.3983573458738086 1101 90-91 5.0757761746471886 1101 92-93 5.428763660566929 1101 94-95 5.333086905239863 1101 96-97 5.195507829036856 1101 98-99 5.196327160672357 1101 100-101 4.375282516740247 1102 1 0.4720399025548758 1102 2 0.5090101798085982 1102 3 0.4327869878350583 1102 4 0.39968932189378137 1102 5 0.34712231512994407 1102 6 0.30692539295057486 1102 7 0.3126450544793755 1102 8 0.24739434423897677 1102 9 0.4881075012876579 1102 10-11 0.41044949738864034 1102 12-13 0.396224977501916 1102 14-15 0.6257649153231739 1102 16-17 0.6297347619622329 1102 18-19 0.5548644256056434 1102 20-21 0.5481276294658528 1102 22-23 0.6348446060881088 1102 24-25 0.6490376343634452 1102 26-27 0.6052316305184675 1102 28-29 0.6888485735580261 1102 30-31 0.706801962967539 1102 32-33 0.8797895303549197 1102 34-35 0.924151787103483 1102 36-37 0.9238912305510283 1102 38-39 1.1907335530803635 1102 40-41 1.2778499179094212 1102 42-43 1.3055811651610725 1102 44-45 1.3065031343884357 1102 46-47 1.433175272122007 1102 48-49 1.7024017715495035 1102 50-51 1.8669275813852195 1102 52-53 1.8154026066712348 1102 54-55 1.6481968561047822 1102 56-57 2.098330762023295 1102 58-59 2.0845210756093735 1102 60-61 2.3065226553798333 1102 62-63 2.419068598433551 1102 64-65 2.6687556034267885 1102 66-67 2.7407374327050285 1102 68-69 2.9549979842885037 1102 70-71 3.1261797905065656 1102 72-73 3.365564101527152 1102 74-75 3.5092904833803225 1102 76-77 3.8220808452729926 1102 78-79 3.9845086502402403 1102 80-81 4.162179838840181 1102 82-83 4.345125484943427 1102 84-85 4.2853891282441054 1102 86-87 4.519289580931964 1102 88-89 4.533251509604067 1102 90-91 4.5463374542240125 1102 92-93 5.174029695033816 1102 94-95 5.1680400761430505 1102 96-97 5.168413492888476 1102 98-99 4.939553472012616 1102 100-101 4.317140782347854 1103 1 0.519841485260919 1103 2 0.29228367913304965 1103 3 0.3014223864067631 1103 4 0.37441620859519276 1103 5 0.3486625583262395 1103 6 0.3460529744985408 1103 7 0.3863142303141629 1103 8 0.47063696149432843 1103 9 0.526493913177255 1103 10-11 0.40200325149016436 1103 12-13 0.5494946165677348 1103 14-15 0.7492989821056568 1103 16-17 0.7624832661120138 1103 18-19 0.665792817807926 1103 20-21 0.7383862667945564 1103 22-23 0.7192047682189653 1103 24-25 0.7844439265850198 1103 26-27 0.9088448326030019 1103 28-29 0.9540637829770517 1103 30-31 0.9357886450752417 1103 32-33 1.028917111902885 1103 34-35 1.1000290308788152 1103 36-37 1.2532774494276921 1103 38-39 1.2785544370795918 1103 40-41 1.3747250385424934 1103 42-43 1.3207365405712252 1103 44-45 1.2582016481888587 1103 46-47 1.5211196843976253 1103 48-49 1.7117223660293277 1103 50-51 1.9197880330354806 1103 52-53 2.0386046912108995 1103 54-55 2.2858150765255445 1103 56-57 2.3194386562522027 1103 58-59 2.6626314790064 1103 60-61 2.7221007291107746 1103 62-63 2.838510791060454 1103 64-65 2.765933754362905 1103 66-67 2.9860781005297703 1103 68-69 3.143459671221528 1103 70-71 3.2083531161193797 1103 72-73 3.5152186083791115 1103 74-75 3.728479829066483 1103 76-77 4.018581520818259 1103 78-79 4.107168368441361 1103 80-81 4.504719889023544 1103 82-83 4.847630792799052 1103 84-85 4.789096906665257 1103 86-87 4.7284866471354015 1103 88-89 4.91124031485402 1103 90-91 5.247846815351206 1103 92-93 5.126347780345533 1103 94-95 4.975816567167946 1103 96-97 5.095850281153293 1103 98-99 4.756513518335719 1103 100-101 3.9282602573526813 1104 1 0.49386907322717377 1104 2 0.49246116050115063 1104 3 0.42875889035180137 1104 4 0.4038147034657271 1104 5 0.369652007842447 1104 6 0.34099129334546063 1104 7 0.4067260453982513 1104 8 0.45275799139648143 1104 9 0.4881856349651841 1104 10-11 0.5912967881799744 1104 12-13 0.5829862982674712 1104 14-15 0.6868803884723889 1104 16-17 0.8394894068944652 1104 18-19 0.8126963692574733 1104 20-21 0.8133462697100597 1104 22-23 0.8499345555693196 1104 24-25 0.8364481540672983 1104 26-27 0.86883991268828 1104 28-29 0.9551840657810473 1104 30-31 0.9104024317696044 1104 32-33 1.0743485155363288 1104 34-35 1.0994626940286594 1104 36-37 1.3495437808112811 1104 38-39 1.47099599031489 1104 40-41 1.5215208985253668 1104 42-43 1.6286388347092355 1104 44-45 1.5537719027488919 1104 46-47 1.5904893886560885 1104 48-49 1.8106981126719504 1104 50-51 1.9950239156135083 1104 52-53 2.071309550993327 1104 54-55 2.006491473690218 1104 56-57 2.4987442194002227 1104 58-59 2.6693342250476917 1104 60-61 2.6392869023282195 1104 62-63 2.975284055879424 1104 64-65 3.0854271853656527 1104 66-67 3.171495291076244 1104 68-69 3.2059183530488227 1104 70-71 3.5273410714392703 1104 72-73 3.721250241608576 1104 74-75 3.74258577123015 1104 76-77 3.8923569176002673 1104 78-79 4.19352198275649 1104 80-81 4.311396395519655 1104 82-83 4.6095229555571215 1104 84-85 4.880281330579688 1104 86-87 5.004172246362792 1104 88-89 5.1581792168019405 1104 90-91 5.315073258981741 1104 92-93 5.397595616494151 1104 94-95 5.28198176697115 1104 96-97 5.257489523850861 1104 98-99 5.046566544083273 1104 100-101 4.078218529187081 1105 1 0.33057560174590606 1105 2 0.24020479953379947 1105 3 0.25985890526236943 1105 4 0.22739818199625716 1105 5 0.2575714375727216 1105 6 0.25223211162063564 1105 7 0.18824177114740337 1105 8 0.23383095027621437 1105 9 0.2751706690949831 1105 10-11 0.17495347549975548 1105 12-13 0.17342554400273968 1105 14-15 0.3682635991727068 1105 16-17 0.5073190512039147 1105 18-19 0.46354249149473503 1105 20-21 0.36741720481268914 1105 22-23 0.35995193723156405 1105 24-25 0.5041468933892688 1105 26-27 0.4647946685644797 1105 28-29 0.5676433504797913 1105 30-31 0.5851674153154747 1105 32-33 0.5415688916326644 1105 34-35 0.7699697653480726 1105 36-37 0.8397680833572068 1105 38-39 0.8473536045399896 1105 40-41 0.8461481168223912 1105 42-43 0.9834401776404036 1105 44-45 1.2144686252940176 1105 46-47 1.143449031418477 1105 48-49 0.9915904296692233 1105 50-51 1.2211659566252777 1105 52-53 1.3537025851679303 1105 54-55 1.630198961945517 1105 56-57 1.849105993183798 1105 58-59 2.1385414518430323 1105 60-61 2.2022019743924623 1105 62-63 2.1529581752633753 1105 64-65 2.101547399545815 1105 66-67 2.2460502316194813 1105 68-69 2.3607775531843487 1105 70-71 2.6507921749263126 1105 72-73 2.9205817861404277 1105 74-75 3.144287851079394 1105 76-77 3.4278685705942458 1105 78-79 3.6205207514095754 1105 80-81 3.8243762900536318 1105 82-83 3.9511193453795705 1105 84-85 4.040507285188561 1105 86-87 3.8134974066514005 1105 88-89 4.000603563169538 1105 90-91 4.255916805120847 1105 92-93 4.347749692364438 1105 94-95 4.252855407256845 1105 96-97 4.048492536065627 1105 98-99 3.607944357577967 1105 100-101 3.255334727314228 1106 1 0.403206166556636 1106 2 0.27307782751341136 1106 3 0.18977139372128704 1106 4 0.19121871643218213 1106 5 0.2741484517431658 1106 6 0.1691041761704355 1106 7 0.10735916239357124 1106 8 0.0832388423720758 1106 9 0.24879276375196469 1106 10-11 0.14917671021535028 1106 12-13 0.22098886944129958 1106 14-15 0.43795101972320083 1106 16-17 0.44791691292497404 1106 18-19 0.4157510205247448 1106 20-21 0.4380884611519207 1106 22-23 0.514356283370482 1106 24-25 0.49452229649097745 1106 26-27 0.6242941533971518 1106 28-29 0.8051766356416366 1106 30-31 0.7823821664963546 1106 32-33 0.8186924515058109 1106 34-35 0.7928555721536341 1106 36-37 0.7967205006293696 1106 38-39 0.8534760671736308 1106 40-41 0.9522642234954759 1106 42-43 0.93051710483455 1106 44-45 1.1406729125566741 1106 46-47 1.186971304042352 1106 48-49 1.40505569936267 1106 50-51 1.610550833453452 1106 52-53 1.6644197382328443 1106 54-55 1.7773772499842693 1106 56-57 1.644887977046352 1106 58-59 2.042746421535135 1106 60-61 2.38263364447128 1106 62-63 2.3799057753864723 1106 64-65 2.541158414759977 1106 66-67 2.6028283617627928 1106 68-69 2.4344053347534 1106 70-71 2.684654056558255 1106 72-73 3.2909814087970837 1106 74-75 3.5003293588470434 1106 76-77 3.146674519773324 1106 78-79 3.5553136140004007 1106 80-81 3.4818097531823717 1106 82-83 3.510446884753076 1106 84-85 3.861359184617193 1106 86-87 3.912786542642195 1106 88-89 3.772661422482024 1106 90-91 4.037956533219546 1106 92-93 4.203521239070717 1106 94-95 4.1305135368649175 1106 96-97 4.116984032459456 1106 98-99 4.108822818231225 1106 100-101 3.506321908345365 1107 1 0.42598755705892444 1107 2 0.3671248722618756 1107 3 0.31249393004685544 1107 4 0.21651893705494274 1107 5 0.2742381802420937 1107 6 0.2943458642239989 1107 7 0.24441092190833302 1107 8 0.3521580481301356 1107 9 0.28626261304668077 1107 10-11 0.4276558603996321 1107 12-13 0.3591215518410067 1107 14-15 0.48594869128474016 1107 16-17 0.5207539415287457 1107 18-19 0.4241838913864626 1107 20-21 0.5623806029937057 1107 22-23 0.573636268447892 1107 24-25 0.5848044427709382 1107 26-27 0.5838110024152812 1107 28-29 0.5701042096274378 1107 30-31 0.6560862489822483 1107 32-33 0.7223303150027078 1107 34-35 0.9541451218387778 1107 36-37 0.9596161582845895 1107 38-39 0.9272631244004685 1107 40-41 0.9590798909275691 1107 42-43 0.9059755563586478 1107 44-45 0.977545054269811 1107 46-47 1.2521071188993318 1107 48-49 1.377588808857162 1107 50-51 1.523273305457515 1107 52-53 1.4725584990147311 1107 54-55 1.7213935812142935 1107 56-57 1.8748114666166202 1107 58-59 2.0500703896573995 1107 60-61 2.3207380254089607 1107 62-63 2.3953611073176084 1107 64-65 2.63274998607001 1107 66-67 2.7272981917610544 1107 68-69 2.7886976695596957 1107 70-71 2.9729282462681006 1107 72-73 3.1052660893443296 1107 74-75 3.258365366025327 1107 76-77 3.270147205646392 1107 78-79 3.443961029240736 1107 80-81 3.5879455423107522 1107 82-83 3.795013743741041 1107 84-85 3.955905846652712 1107 86-87 3.977043564942459 1107 88-89 4.115658418667756 1107 90-91 4.0533983576347055 1107 92-93 3.95765791583586 1107 94-95 3.940730847068238 1107 96-97 4.203655812685739 1107 98-99 4.363399614891655 1107 100-101 3.564334598978226 1108 1 0.3565824801151223 1108 2 0.36760672087118706 1108 3 0.3437894533021293 1108 4 0.1389445104819842 1108 5 0.20531207233424453 1108 6 0.1927196411652048 1108 7 0.21226048531701736 1108 8 0.2524665784182858 1108 9 0.26410397607033076 1108 10-11 0.3762399581797027 1108 12-13 0.38579535984960955 1108 14-15 0.4839645052617385 1108 16-17 0.5672974456431703 1108 18-19 0.5328711482824815 1108 20-21 0.5817996458683083 1108 22-23 0.5530641164179357 1108 24-25 0.6169996727256759 1108 26-27 0.6270666736550297 1108 28-29 0.6149442861217125 1108 30-31 0.8327183191747238 1108 32-33 0.8594659969800702 1108 34-35 0.9285138793636634 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48-49 1.085955826589128 2311 50-51 1.2115637096489422 2311 52-53 1.247046745339972 2311 54-55 1.56815662174877 2311 56-57 1.5929845156091318 2311 58-59 1.6702618861310796 2311 60-61 1.8337841665384893 2311 62-63 1.9556554244169284 2311 64-65 2.4048297756444157 2311 66-67 2.452224584452974 2311 68-69 2.5279458498012204 2311 70-71 3.040184020583876 2311 72-73 3.4008524274862104 2311 74-75 3.5349371503711318 2311 76-77 3.5114053699970675 2311 78-79 3.809689765619243 2311 80-81 3.7240149387575237 2311 82-83 3.925375703740528 2311 84-85 4.0923964093254455 2311 86-87 4.220429453057214 2311 88-89 4.354413747655315 2311 90-91 4.651609248846466 2311 92-93 4.880714250389481 2311 94-95 4.7493271500964696 2311 96-97 4.667121678331178 2311 98-99 4.60581435109075 2311 100-101 4.2008215573758125 2312 1 -0.3240991637510575 2312 2 -0.32398010923381904 2312 3 -0.15821169020845005 2312 4 -0.07493380325826848 2312 5 -0.07738605481855387 2312 6 -0.06296653490753101 2312 7 -0.059713743859234114 2312 8 -0.07767329244868648 2312 9 -0.025050802269902306 2312 10-11 -0.18508428285001344 2312 12-13 -0.0578239905806015 2312 14-15 -0.039100939800945866 2312 16-17 -0.0988741646149549 2312 18-19 -0.1353783043664265 2312 20-21 -0.16303015234390728 2312 22-23 -0.00770231457398296 2312 24-25 0.014704284420155034 2312 26-27 0.003937080437999896 2312 28-29 0.10125004969392393 2312 30-31 0.2093865198189988 2312 32-33 0.15297169361508622 2312 34-35 0.2838737365642956 2312 36-37 0.2727310744956739 2312 38-39 0.4130494098166224 2312 40-41 0.43094127360365064 2312 42-43 0.5337958497234894 2312 44-45 0.6599657224333768 2312 46-47 0.596511057865392 2312 48-49 0.9218911035657626 2312 50-51 1.0193608899990423 2312 52-53 1.0792970729203049 2312 54-55 1.1938322012937093 2312 56-57 1.3679654799423986 2312 58-59 1.2630671286459147 2312 60-61 1.4669857505167379 2312 62-63 1.657474658915259 2312 64-65 1.7720080267694343 2312 66-67 1.9491888676580267 2312 68-69 2.292911901764416 2312 70-71 2.5840108438801934 2312 72-73 2.7878762943041693 2312 74-75 2.445872624949846 2312 76-77 2.6841370298896408 2312 78-79 3.1076982879772608 2312 80-81 3.479536160342345 2312 82-83 3.8307079518725295 2312 84-85 4.02808099513393 2312 86-87 4.375899803085144 2312 88-89 4.423253590732919 2312 90-91 4.635228036676285 2312 92-93 4.615923844716811 2312 94-95 4.820868652680225 2312 96-97 4.689891475424584 2312 98-99 4.441416668567907 2312 100-101 4.0323721540462625 2313 1 -0.39485624581871903 2313 2 -0.35878682490227476 2313 3 -0.34875273160755427 2313 4 -0.23754054486909837 2313 5 -0.2917759980922199 2313 6 -0.2552779224102437 2313 7 -0.24058694221515253 2313 8 -0.2394498361301558 2313 9 -0.3005747635396645 2313 10-11 -0.29031896565617643 2313 12-13 -0.23446000502507758 2313 14-15 -0.3140191235742691 2313 16-17 -0.06979022254338219 2313 18-19 -0.24494876891355943 2313 20-21 -0.13449480523224366 2313 22-23 -0.22637768952429127 2313 24-25 -0.13269936563364837 2313 26-27 -0.10857408014253878 2313 28-29 -0.21998220453627226 2313 30-31 -0.19370884236194996 2313 32-33 -0.0895860097660659 2313 34-35 -0.1038448841508739 2313 36-37 0.04108009903938381 2313 38-39 0.10556829424419334 2313 40-41 0.08307436091192244 2313 42-43 0.054558681579294444 2313 44-45 0.29167203040296386 2313 46-47 0.38340383241041565 2313 48-49 0.6110477809828865 2313 50-51 0.601455461084818 2313 52-53 0.7705416487257466 2313 54-55 0.8503803270204457 2313 56-57 0.8969519804489465 2313 58-59 0.943478136536477 2313 60-61 1.1834194438968346 2313 62-63 1.2670230493214625 2313 64-65 1.482020247434317 2313 66-67 1.5461862168690423 2313 68-69 1.8703380022707137 2313 70-71 2.0114626029474643 2313 72-73 2.240836089725235 2313 74-75 2.5877397651103387 2313 76-77 2.505129411788257 2313 78-79 2.7536583943283723 2313 80-81 2.886142151852841 2313 82-83 3.063443188108934 2313 84-85 3.336916306695713 2313 86-87 3.4253878196647136 2313 88-89 3.4174044689578693 2313 90-91 3.4419780777236753 2313 92-93 3.806967545839669 2313 94-95 3.9825045525494005 2313 96-97 4.0253919287853925 2313 98-99 3.896586611072131 2313 100-101 3.689624655098987 2314 1 -0.31094219547453505 2314 2 -0.3794581140730102 2314 3 -0.31554917045957964 2314 4 -0.18908836807904805 2314 5 -0.15049969108589778 2314 6 -0.27616030250649004 2314 7 -0.199252174638481 2314 8 -0.10435885373822629 2314 9 -0.2000061598901155 2314 10-11 -0.32046190866022073 2314 12-13 -0.19742486319260877 2314 14-15 -0.1036547994627739 2314 16-17 -0.1637088612111981 2314 18-19 -0.2324238070110738 2314 20-21 -0.18489286383661607 2314 22-23 -0.1261668789852095 2314 24-25 -0.048480495186403516 2314 26-27 -0.07094752322179687 2314 28-29 -0.05310000749230426 2314 30-31 0.12558060235075885 2314 32-33 0.03466963936956802 2314 34-35 0.16163199608546108 2314 36-37 0.32635146002910886 2314 38-39 0.2874952259414627 2314 40-41 0.21464599293517495 2314 42-43 0.38250756072453385 2314 44-45 0.5877647053275439 2314 46-47 0.817491291746876 2314 48-49 0.7715981515035395 2314 50-51 0.7496365843088455 2314 52-53 0.8701375117441543 2314 54-55 1.122423156447958 2314 56-57 1.3400379415204924 2314 58-59 1.4964441342745545 2314 60-61 1.6637613511133544 2314 62-63 1.6996862457387643 2314 64-65 1.827340367623961 2314 66-67 2.0252338403036596 2314 68-69 2.4286653890752063 2314 70-71 2.8326059026431025 2314 72-73 2.921127367163802 2314 74-75 3.2970095807702826 2314 76-77 3.258568501541756 2314 78-79 3.4359183850475787 2314 80-81 3.548051495309995 2314 82-83 3.7833987308379236 2314 84-85 4.129910611446519 2314 86-87 4.286288913552312 2314 88-89 4.464222393421366 2314 90-91 4.440888676310649 2314 92-93 4.67075241414517 2314 94-95 4.534828164952014 2314 96-97 4.76059328329217 2314 98-99 4.4604224896800275 2314 100-101 4.029149287947979 2315 1 -0.6155051368537627 2315 2 -0.3584971327884432 2315 3 -0.35298096267339574 2315 4 -0.15751604166405286 2315 5 -0.24035921837614893 2315 6 -0.21205049364294837 2315 7 -0.183623166467342 2315 8 -0.18479722831248324 2315 9 -0.3380434759228734 2315 10-11 -0.23584980377734155 2315 12-13 -0.23661860692190828 2315 14-15 -0.15473796531532003 2315 16-17 -0.25698671890930314 2315 18-19 -0.2704609748717246 2315 20-21 -0.2554997415781486 2315 22-23 -0.13304549102748808 2315 24-25 -0.1446830849754548 2315 26-27 -0.0994813954570688 2315 28-29 -0.09782621971142902 2315 30-31 -0.0741975284470513 2315 32-33 0.13018415495699998 2315 34-35 0.18409247904872927 2315 36-37 0.24000787151933167 2315 38-39 0.22695479042290856 2315 40-41 0.14872089059918636 2315 42-43 0.5143690635193394 2315 44-45 0.4347119988436958 2315 46-47 0.5140651082175722 2315 48-49 0.8586889904484281 2315 50-51 0.8884138347791506 2315 52-53 0.9834497194399532 2315 54-55 1.1818092386794063 2315 56-57 1.3850118789737138 2315 58-59 1.6701599961166593 2315 60-61 1.7532402613149536 2315 62-63 1.9379054985920483 2315 64-65 2.1658623175614267 2315 66-67 2.271788051753031 2315 68-69 2.4985316841262417 2315 70-71 2.658451821807443 2315 72-73 2.9454498178118413 2315 74-75 3.1966159430581094 2315 76-77 3.393461883379228 2315 78-79 3.7631771252105715 2315 80-81 3.8735142969814476 2315 82-83 4.021580940665551 2315 84-85 4.2595938917249825 2315 86-87 4.408981711850998 2315 88-89 4.720388066131051 2315 90-91 4.745291221388769 2315 92-93 5.06564301405141 2315 94-95 5.212061910333443 2315 96-97 5.419708636570579 2315 98-99 5.279402484038371 2315 100-101 4.695521462560654 2316 1 -0.7452240959240655 2316 2 -0.6246347589794148 2316 3 -0.5033492101558821 2316 4 -0.4441847261251439 2316 5 -0.372540517205195 2316 6 -0.32703009500243496 2316 7 -0.37248872460819626 2316 8 -0.3983303232938482 2316 9 -0.4444679442025148 2316 10-11 -0.3989947886123488 2316 12-13 -0.24829974495797558 2316 14-15 -0.4053354216332252 2316 16-17 -0.30312957875455737 2316 18-19 -0.45305008887081044 2316 20-21 -0.5094525693278698 2316 22-23 -0.4690185093326633 2316 24-25 -0.4708319666759522 2316 26-27 -0.4080155623917179 2316 28-29 -0.46140282338507177 2316 30-31 -0.5274580004848346 2316 32-33 -0.25785584151185503 2316 34-35 -0.1327706072939563 2316 36-37 -0.007238154371492556 2316 38-39 -0.242363699510193 2316 40-41 -0.019965146893952124 2316 42-43 0.17773789742332724 2316 44-45 0.2593625105437525 2316 46-47 0.4492261219070528 2316 48-49 0.5381402764770868 2316 50-51 0.35861510977300526 2316 52-53 0.604279348228836 2316 54-55 0.9420038297292237 2316 56-57 1.0714505663984397 2316 58-59 1.1908321423563137 2316 60-61 1.181417026412145 2316 62-63 1.6820144847134024 2316 64-65 1.9796884656549238 2316 66-67 2.103448822676615 2316 68-69 2.2968917532356983 2316 70-71 2.6597484123654276 2316 72-73 3.0381335789806485 2316 74-75 3.200738234011453 2316 76-77 3.471538704596334 2316 78-79 3.7719316731300374 2316 80-81 3.8738138133413464 2316 82-83 4.103907741389431 2316 84-85 4.348190830983064 2316 86-87 4.630672686634874 2316 88-89 4.882504448730092 2316 90-91 5.129727563127474 2316 92-93 5.269984897788618 2316 94-95 5.5228850128184845 2316 96-97 5.738971794797191 2316 98-99 5.656674079167921 2316 100-101 5.5326428896457625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5064.0 3 1768.0 4 1947.0 5 2273.0 6 2787.0 7 3315.0 8 4127.0 9 5479.0 10 7007.0 11 8713.0 12 11016.0 13 13807.0 14 16518.0 15 19797.0 16 23457.0 17 28985.0 18 29458.0 19 26822.0 20 28063.0 21 30849.0 22 33742.0 23 37538.0 24 41829.0 25 45945.0 26 50444.0 27 55128.0 28 60315.0 29 66201.0 30 72551.0 31 81874.0 32 91699.0 33 107928.0 34 130504.0 35 168618.0 36 199280.0 37 219086.0 38 127373.0 39 9335.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.483599440632695 18.44914018204122 14.349671108764314 22.717589268561774 2 15.621221980832994 19.704436921750855 40.763707275131495 23.91063382228465 3 19.523295844415195 24.254023297716277 29.523900188095286 26.698780669773242 4 12.608807025255905 16.532731816385454 36.787242792411945 34.0712183659467 5 13.493054734649624 37.49635606914402 34.01690356006027 14.99368563614608 6 32.12768529483817 35.81865855341492 17.273395402276716 14.78026074947019 7 28.565554068383648 33.23495896136342 20.074147076013855 18.125339894239083 8 26.730784244315565 34.76599722154787 19.238695560572502 19.264522973564066 9 26.643258892619066 16.75226225833327 20.11048996970109 36.49398887934658 10-11 25.25364495561564 26.702162388918786 26.53979004692034 21.504402608545227 12-13 26.246538599643817 24.115195617747503 27.344213923565647 22.294051859043034 14-15 22.927841942038285 25.403406052828576 26.060448080419206 25.608303924713933 16-17 21.91891175058259 28.639752396413854 26.95255726207244 22.488778590931112 18-19 21.88425631712104 26.614935336282613 29.89476121358664 21.60604713300971 20-21 22.52326592516613 25.787688338747166 30.288619403583716 21.400426332502988 22-23 22.112241133159017 25.87018827165445 30.11394686044584 21.903623734740695 24-25 21.872177109456352 26.094753388003483 30.270157811782163 21.762911690758006 26-27 21.53438805398638 26.439785675971546 30.107616589094803 21.91820968094727 28-29 21.549958557257828 26.746022833614074 30.044571000775385 21.659447608352718 30-31 21.75178753830439 26.41238254656691 29.95018423543631 21.885645679692388 32-33 21.482791878159016 26.358121132407337 29.892769602108725 22.266317387324925 34-35 22.089822735313682 26.55213553999739 29.742985456466727 21.615056268222197 36-37 22.18113114921104 26.165776855241447 29.736564658545838 21.916527337001675 38-39 21.749707407235128 26.488480479825398 29.678663905681674 22.083148207257796 40-41 22.120907862208195 26.36814484050607 29.761828384701584 21.74911891258415 42-43 21.945495605795067 26.36704736588292 29.533639004757223 22.153818023564785 44-45 21.858941047404585 26.677455502734098 29.54553230983741 21.91807114002391 46-47 22.26910334440355 26.862268146295836 28.870573082287198 21.99805542701342 48-49 22.118295367908015 26.947298579009892 28.74724789428492 22.187158158797168 50-51 22.256883853418188 27.245218298775065 28.52789468281667 21.970003164990075 52-53 22.445098851069982 27.00382477714639 28.61301643111859 21.938059940665035 54-55 22.018059254305317 27.173134099358776 28.726248145947388 22.08255850038852 56-57 22.16246718135778 27.412594553241448 28.387089703486808 22.03784856191396 58-59 22.07207038642395 26.998622892908543 28.525068053467677 22.404238667199834 60-61 21.95616198590197 27.12695307331471 28.7673569020374 22.149528038745917 62-63 22.0464253446884 27.06682063372413 28.83447472106836 22.052279300519103 64-65 21.78903079908688 27.087264970516063 28.760471743000572 22.363232487396488 66-67 21.809025615255283 27.28274612216095 28.560782437391914 22.34744582519185 68-69 22.214930827683467 26.913478738293524 28.70784786901792 22.163742565005084 70-71 22.04894996280085 27.007678021919563 28.47294754398736 22.470424471292226 72-73 22.06675005366512 27.142441264427763 28.343818192462507 22.446990489444612 74-75 22.089076981638858 27.440533726246635 28.151049574652813 22.319339717461688 76-77 22.200332743832156 27.136291317652745 28.225861637587222 22.437514300927877 78-79 22.299574449885593 27.247209332164317 28.123676838526183 22.329539379423906 80-81 22.435874303289545 27.334492924701674 27.92455331808062 22.305079453928162 82-83 22.50489053697802 27.413685163295014 27.778250035151984 22.303174264574977 84-85 22.397001102347218 27.351469136950872 27.843649528428475 22.407880232273435 86-87 22.596795468912255 27.17850780417853 27.777262503458054 22.44743422345116 88-89 22.56952084321969 27.207630148980392 27.609990843286525 22.61285816451339 90-91 22.501849313621936 27.30581404954369 27.638088990014108 22.554247646820265 92-93 22.689756735739593 26.91737580092541 27.764728085672992 22.62813937766201 94-95 22.522794451051016 26.800510321934862 27.834655479223525 22.8420397477906 96-97 22.83760196611168 26.42469177916479 27.767878840212255 22.969827414511276 98-99 22.57694821642676 26.633098660264952 27.39753493028646 23.39241819302183 100-101 23.4683856867018 25.66170426376336 27.613904432447246 23.25600561708759 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2695.0 1 1735.0 2 1216.5 3 1558.5 4 1122.0 5 949.0 6 1769.5 7 3734.5 8 5820.0 9 6358.0 10 4753.0 11 3021.0 12 2688.0 13 2773.5 14 2840.0 15 2878.0 16 2949.0 17 3155.5 18 3400.0 19 3619.0 20 4018.5 21 4668.0 22 5538.5 23 6649.5 24 8134.5 25 9965.5 26 12181.0 27 14865.0 28 17268.0 29 19429.5 30 22003.5 31 25644.0 32 30261.0 33 33171.0 34 34587.0 35 36773.5 36 40247.0 37 43761.5 38 46447.0 39 48923.5 40 51495.5 41 54583.0 42 56475.0 43 57785.5 44 60001.5 45 62936.0 46 64491.5 47 65141.5 48 66308.5 49 66060.0 50 64964.0 51 63702.0 52 62548.5 53 62403.0 54 62642.0 55 59745.0 56 55201.0 57 50991.5 58 47120.5 59 42143.0 60 35481.0 61 30040.5 62 25306.0 63 19633.5 64 14485.5 65 11171.0 66 8640.0 67 6756.5 68 5127.0 69 3831.5 70 2922.5 71 2171.0 72 1672.0 73 1280.0 74 940.0 75 709.0 76 494.0 77 329.0 78 241.5 79 163.0 80 96.5 81 63.0 82 40.5 83 22.0 84 14.5 85 13.5 86 11.0 87 7.5 88 5.0 89 3.0 90 2.0 91 2.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3799765000465081 2 0.024002454772211895 3 0.04517165764480857 4 0.03758068085716027 5 0.05853605339771052 6 0.059231001976861423 7 0.03073811023167447 8 0.02865326449422177 9 0.03250221047105753 10-11 0.029802602528971336 12-13 0.02958877219692491 14-15 0.023628251691130638 16-17 0.029722416154453927 18-19 0.02493796247491503 20-21 0.039558611428589754 22-23 0.025231979181478875 24-25 0.04720304579924967 26-27 0.04078813583785673 28-29 0.0316468891428718 30-31 0.04020010242472905 32-33 0.03723320656758482 34-35 0.03581658061777721 36-37 0.036164054907352666 38-39 0.015769986988424294 40-41 0.012081413760623357 42-43 0.011974498594600142 44-45 0.010156940772205478 46-47 0.016491664359081 48-49 0.013685141250971592 50-51 0.009515449776066184 52-53 0.0040360475173763875 54-55 0.0041429626833996034 56-57 0.007216773706567051 58-59 0.0028867094826268203 60-61 0.00515865676062015 62-63 0.005987249297300071 64-65 0.007056400957532226 66-67 0.004116233891893799 68-69 0.005131927969114347 70-71 0.01622437644402296 72-73 0.013177294212361317 74-75 0.009087789111973322 76-77 0.007163316123555442 78-79 0.00652182512741615 80-81 0.00831265415830501 82-83 0.0103173135212403 84-85 0.0047042673050214845 86-87 5.078470386102739E-4 88-89 0.022960031903485542 90-91 0.018897255594603347 92-93 0.01216160013514077 94-95 0.024055912355223502 96-97 0.018790340428580136 98-99 0.016545121942092608 100-101 0.11001570583788882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1870642.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.44171531260565 #Duplication Level Percentage of deduplicated Percentage of total 1 80.11792678442417 49.22582848924773 2 12.116653776989637 14.889359838144104 3 3.5078988160531814 6.46593961204098 4 1.4628513539834196 3.5952038574443597 5 0.7355191757207793 2.2595779900799244 6 0.4499519552542383 1.6587491963448713 7 0.28665182896049046 1.2328666048179728 8 0.20457927691184755 1.0055761352701162 9 0.15397230047708418 0.8514290026745984 >10 0.8821625043082617 9.797352002007703 >50 0.052779028541151005 2.2061289561241892 >100 0.02486614140244468 2.8655132985601184 >500 0.0025296802547389837 1.0882632374328076 >1k 0.0013956856577870245 1.3363583138217001 >5k 8.723035361168903E-5 0.33449154714691703 >10k+ 1.7446070722337807E-4 1.1873619188419804 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12111 0.6474247878535818 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10043 0.5368745061855769 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6241 0.3336287755754441 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2519 0.13465965160624 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2327 0.12439579566801132 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2016 0.1077704873514013 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1984 0.10605984469502984 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.0691516602321556E-4 5 0.0 0.0 0.0 0.0 1.0691516602321556E-4 6 5.345758301160778E-5 0.0 0.0 0.0 1.0691516602321556E-4 7 5.345758301160778E-5 0.0 0.0 0.0 1.0691516602321556E-4 8 5.345758301160778E-5 0.0 0.0 0.0 1.6037274903482333E-4 9 5.345758301160778E-5 0.0 0.0 0.0 2.1383033204643113E-4 10-11 5.345758301160778E-5 0.0 0.0 0.0 2.1383033204643113E-4 12-13 5.345758301160778E-5 0.0 0.0 0.0 2.1383033204643113E-4 14-15 1.6037274903482333E-4 0.0 0.0 0.0 2.40559123552235E-4 16-17 1.8710154054062724E-4 0.0 0.0 0.0 3.2074549806964666E-4 18-19 2.40559123552235E-4 0.0 0.0 0.0 3.2074549806964666E-4 20-21 2.672879150580389E-4 0.0 0.0 0.0 3.7420308108125443E-4 22-23 2.672879150580389E-4 0.0 0.0 0.0 3.7420308108125443E-4 24-25 3.7420308108125443E-4 0.0 0.0 1.3364395752901945E-4 4.2766066409286225E-4 26-27 3.7420308108125443E-4 0.0 0.0 7.48406162162509E-4 5.880334131276856E-4 28-29 3.7420308108125443E-4 0.0 0.0 0.004410250598457642 5.880334131276856E-4 30-31 3.7420308108125443E-4 0.0 0.0 0.014380089830122494 5.880334131276856E-4 32-33 3.7420308108125443E-4 0.0 0.0 0.03207454980696467 5.880334131276856E-4 34-35 4.0093187258705837E-4 0.0 0.0 0.05805493515060605 6.414909961392933E-4 36-37 4.8111824710447E-4 0.0 0.0 0.0996182059421311 6.414909961392933E-4 38-39 5.880334131276856E-4 0.0 0.0 0.1684448440695761 6.414909961392933E-4 40-41 5.880334131276856E-4 0.0 0.0 0.23938305672597965 6.414909961392933E-4 42-43 6.682197876450972E-4 0.0 0.0 0.30559027328585586 6.414909961392933E-4 44-45 8.018637451741167E-4 0.0 0.0 0.38828915420481314 6.414909961392933E-4 46-47 8.285925366799206E-4 0.0 0.0 0.4870253100272527 6.414909961392933E-4 48-49 8.553213281857245E-4 0.0 0.0 0.5874186509230521 6.414909961392933E-4 50-51 8.553213281857245E-4 0.0 0.0 0.6965523066412493 6.414909961392933E-4 52-53 8.553213281857245E-4 0.0 0.0 0.8005540343903323 6.682197876450972E-4 54-55 8.553213281857245E-4 0.0 0.0 0.913590093668377 6.949485791509011E-4 56-57 8.553213281857245E-4 0.0 0.0 1.0442404265487464 6.949485791509011E-4 58-59 8.820501196915284E-4 0.0 0.0 1.1828559392978453 6.949485791509011E-4 60-61 9.087789111973323E-4 0.0 0.0 1.331174003363551 6.949485791509011E-4 62-63 9.087789111973323E-4 0.0 0.0 1.474841257707247 6.949485791509011E-4 64-65 9.087789111973323E-4 0.0 0.0 1.6207270017459248 6.949485791509011E-4 66-67 9.6223649420894E-4 0.0 0.0 1.7880225077807512 8.018637451741167E-4 68-69 9.6223649420894E-4 2.672879150580389E-5 0.0 1.971916593340682 8.018637451741167E-4 70-71 9.6223649420894E-4 5.345758301160778E-5 0.0 2.1705649718118165 8.018637451741167E-4 72-73 9.88965285714744E-4 5.345758301160778E-5 0.0 2.3679036394991666 8.553213281857245E-4 74-75 0.0010156940772205478 5.345758301160778E-5 0.0 2.55604760290852 8.553213281857245E-4 76-77 0.0010156940772205478 8.018637451741168E-5 0.0 2.7603090275958735 8.553213281857245E-4 78-79 0.0010156940772205478 1.0691516602321556E-4 0.0 2.991780362036135 8.553213281857245E-4 80-81 0.0010691516602321556 1.0691516602321556E-4 0.0 3.234664890449375 8.553213281857245E-4 82-83 0.0010691516602321556 1.0691516602321556E-4 0.0 3.4766673687429237 0.0010156940772205478 84-85 0.0010691516602321556 1.0691516602321556E-4 0.0 3.7212090822295236 0.0010156940772205478 86-87 0.0010691516602321556 1.0691516602321556E-4 0.0 3.973368501295277 0.0010156940772205478 88-89 0.0010691516602321556 1.0691516602321556E-4 0.0 4.23547103080119 0.0010156940772205478 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4930 0.0 56.88424 1 GTATCAA 8515 0.0 39.936172 1 TGGTATC 2095 0.0 31.305456 2 TATCAAC 10840 0.0 30.865147 2 ATCAACG 10860 0.0 30.720781 3 TCAACGC 11015 0.0 30.417925 4 CAACGCA 11120 0.0 30.00249 5 AACGCAG 11485 0.0 29.086483 6 GTGGTAT 2350 0.0 28.819208 1 ACGCAGA 12880 0.0 25.636251 7 CGCAGAG 13030 0.0 25.158817 8 GTGTACG 175 1.4297984E-7 24.52822 1 TACGCTC 140 1.7729306E-5 23.762655 4 TACATGG 8330 0.0 23.505917 2 GTACATG 8515 0.0 23.35678 1 GCAGAGT 13940 0.0 23.139698 9 GAGTACT 8920 0.0 22.767239 12-13 GTATAGG 520 0.0 22.012505 1 ACATGGG 8570 0.0 21.738533 3 CAGAGTA 13590 0.0 21.672615 10-11 >>END_MODULE