FastQCFastQC Report
Thu 26 May 2016
SRR938163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938163_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1870642
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117890.630211446123844No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101030.5400819611662734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66520.35559984219321494No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24550.13123836629349708No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24070.12867240230893992No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20940.11194017882630669No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA19450.10397499895757713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.054.5160981
GTATCAA82450.042.742361
ATCAACG104350.033.5396233
TATCAAC105100.033.485742
TCAACGC105150.033.2844474
CAACGCA107900.032.4802135
AACGCAG111600.031.4924226
TGGTATC19800.030.2646962
ACGCAGA127200.027.630147
GTGGTAT23100.027.5882241
CGCAGAG126950.027.5349068
GCAGAGT137450.024.9822779
GTACATG81800.024.6515061
TACATGG80450.024.5922492
ACATGGG79700.023.746393
GAGTACT88150.023.70657712-13
GTACTTT94100.022.08141514-15
CAGAGTA133450.021.65611310-11
AGTACTT90900.021.5264212-13
CGTATAC1353.7689856E-421.1344183
Produced by FastQC (version 0.11.5)