FastQCFastQC Report
Thu 26 May 2016
SRR938162_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938162_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861413
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120250.6460146136295384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97130.5218078953998925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62150.3338861391856616No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25490.13693898130076454No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25040.13452146299612178No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19950.1071766448391625No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18870.10137460090801986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47450.057.4666021
GTATCAA87400.043.3288731
ATCAACG110250.034.097163
TATCAAC111700.033.9098172
TCAACGC111450.033.687394
CAACGCA113300.033.0953875
AACGCAG117300.032.0030256
TGGTATC18850.030.7587572
GTGGTAT20700.029.2987441
ACGCAGA130550.028.6349497
CGCAGAG130400.028.4493248
GCAGAGT140250.025.9425569
GAGTACT88800.024.49882512-13
GTACATG88450.024.0797881
TACATGG86800.023.8719042
GTACTTT93950.023.13371514-15
CAGAGTA135250.022.95035210-11
ACATGGG85500.022.6785013
TAGCGCG3007.2759576E-1222.1782424
AGAGTAC133300.021.14811110-11
Produced by FastQC (version 0.11.5)