##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938162_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1861413 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.836209911502714 33.0 31.0 34.0 30.0 34.0 2 32.01902425737867 34.0 31.0 34.0 30.0 34.0 3 32.09603188545476 34.0 31.0 34.0 30.0 34.0 4 35.62094709771555 37.0 35.0 37.0 33.0 37.0 5 35.63219822790536 37.0 35.0 37.0 33.0 37.0 6 35.66028710447386 37.0 35.0 37.0 33.0 37.0 7 35.59623146502147 37.0 35.0 37.0 33.0 37.0 8 35.5729163812652 37.0 35.0 37.0 33.0 37.0 9 37.2004966119824 39.0 37.0 39.0 34.0 39.0 10-11 37.115462554521756 39.0 37.0 39.0 33.5 39.0 12-13 37.00691651986958 39.0 37.0 39.0 33.0 39.0 14-15 38.17937797791248 40.0 38.0 41.0 33.0 41.0 16-17 38.0269698342066 40.0 38.0 41.0 33.0 41.0 18-19 38.17839324212305 40.0 38.0 41.0 33.5 41.0 20-21 38.15928141685913 40.0 38.0 41.0 33.0 41.0 22-23 38.06850951400898 40.0 38.0 41.0 33.0 41.0 24-25 37.95663724278277 40.0 38.0 41.0 33.0 41.0 26-27 37.775894441480744 40.0 38.0 41.0 33.0 41.0 28-29 37.55654360424043 40.0 37.5 41.0 32.0 41.0 30-31 37.317481665809794 40.0 37.0 41.0 31.5 41.0 32-33 37.04346241269401 40.0 36.0 41.0 31.0 41.0 34-35 36.79807866389673 39.5 36.0 41.0 30.5 41.0 36-37 36.533362021217215 39.0 35.5 41.0 30.0 41.0 38-39 36.2437253312403 39.0 35.0 40.5 30.0 41.0 40-41 36.014635387203164 39.0 35.0 40.0 29.0 41.0 42-43 35.82834115803425 39.0 35.0 40.0 28.5 41.0 44-45 35.536635072388556 39.0 35.0 40.0 27.0 41.0 46-47 35.126733024858 38.5 35.0 40.0 25.5 41.0 48-49 34.71359311447809 38.0 34.0 40.0 24.0 41.0 50-51 34.007085477537764 37.5 33.5 39.5 23.0 40.5 52-53 34.13823262220689 38.0 33.5 40.0 23.0 40.5 54-55 34.244072916649884 38.0 34.0 40.0 23.0 41.0 56-57 33.94898633457486 38.0 34.0 40.0 21.5 41.0 58-59 33.63306423668472 37.5 33.0 40.0 20.0 41.0 60-61 33.20986342096032 37.0 33.0 40.0 18.5 41.0 62-63 32.673119291634904 36.5 32.0 39.5 14.0 41.0 64-65 32.15991695556011 36.0 31.5 39.0 8.5 40.5 66-67 31.634176295104847 35.0 31.0 39.0 7.0 40.0 68-69 31.034678494240666 35.0 30.5 38.0 2.0 40.0 70-71 30.492029979375886 35.0 29.5 37.5 2.0 40.0 72-73 29.864041456678343 34.5 29.0 37.0 2.0 39.0 74-75 29.190403741673663 34.0 28.5 36.0 2.0 39.0 76-77 28.53269559200457 34.0 27.0 36.0 2.0 38.5 78-79 27.810366640826082 33.5 26.0 35.0 2.0 37.5 80-81 27.15457235981483 33.0 25.5 35.0 2.0 37.0 82-83 26.434644541539143 32.5 23.5 35.0 2.0 36.0 84-85 25.784031539481028 32.0 21.0 35.0 2.0 36.0 86-87 25.100982694329524 31.5 19.0 34.5 2.0 35.0 88-89 24.293157133854766 31.0 12.0 34.0 2.0 35.0 90-91 23.470489085442082 31.0 4.5 34.0 2.0 35.0 92-93 22.591806869297677 30.0 2.0 34.0 2.0 35.0 94-95 21.64826747207632 29.0 2.0 34.0 2.0 35.0 96-97 20.46612707658107 29.0 2.0 34.0 2.0 35.0 98-99 18.996819888976816 26.0 2.0 33.5 2.0 35.0 100-101 15.3839164118871 11.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3390218130889515 1101 2 0.34747972759662815 1101 3 0.3831282056130334 1101 4 0.3087571687937256 1101 5 0.2519068444792083 1101 6 0.16691153292396876 1101 7 0.2182062188224947 1101 8 0.3449949594588162 1101 9 0.3555108981265178 1101 10-11 0.3378956244728357 1101 12-13 0.45520136791765253 1101 14-15 0.5329208138608124 1101 16-17 0.6793254510783271 1101 18-19 0.4661410619263009 1101 20-21 0.4934922086331355 1101 22-23 0.5880918794759609 1101 24-25 0.5663001018734874 1101 26-27 0.5238929429456576 1101 28-29 0.5989291221030157 1101 30-31 0.6376133051763304 1101 32-33 0.5929376582971528 1101 34-35 0.7224465877776041 1101 36-37 0.8986659215686288 1101 38-39 0.8274498024993946 1101 40-41 1.0147869329703383 1101 42-43 1.1537198110830147 1101 44-45 0.9315523983236957 1101 46-47 1.123644658270635 1101 48-49 1.0066114653610398 1101 50-51 1.1156985665163361 1101 52-53 1.0470648345589098 1101 54-55 1.1203340617122777 1101 56-57 1.0998405944906366 1101 58-59 1.3251380495049219 1101 60-61 1.3611203409912562 1101 62-63 1.2307884276634056 1101 64-65 1.1607897836067522 1101 66-67 1.2916817644125587 1101 68-69 1.5390014233889104 1101 70-71 1.5508356302885353 1101 72-73 1.725494707131471 1101 74-75 2.00413657659519 1101 76-77 2.0234566898778894 1101 78-79 1.9076276163874866 1101 80-81 2.185623851414743 1101 82-83 2.2186400193705467 1101 84-85 2.4190016192259094 1101 86-87 2.663304109075586 1101 88-89 2.9567509921476898 1101 90-91 3.140761783386239 1101 92-93 3.3828585657458596 1101 94-95 3.6916602240246412 1101 96-97 3.9535174732979996 1101 98-99 4.16343838991423 1101 100-101 4.240830556424349 1102 1 0.4150817101235553 1102 2 0.31648154866507383 1102 3 0.33054434476266437 1102 4 0.18788120337735137 1102 5 0.2708129807203221 1102 6 0.2756383967402769 1102 7 0.12826470420485236 1102 8 0.26429402410025915 1102 9 0.2296270871100745 1102 10-11 0.2546918778514424 1102 12-13 0.41307225546158577 1102 14-15 0.4001621452600759 1102 16-17 0.580476262593109 1102 18-19 0.3811936839499239 1102 20-21 0.5132844252791458 1102 22-23 0.4620509712018688 1102 24-25 0.29353257802637955 1102 26-27 0.38463011441694306 1102 28-29 0.4125021028567488 1102 30-31 0.4943061485440623 1102 32-33 0.4599177784543258 1102 34-35 0.5097785835611717 1102 36-37 0.47963677521067183 1102 38-39 0.45369338105636103 1102 40-41 0.6427533941878849 1102 42-43 0.7144267857936981 1102 44-45 0.7557068427125628 1102 46-47 0.6358837943839148 1102 48-49 0.8368901092115522 1102 50-51 0.7740459330068248 1102 52-53 0.7711131271451492 1102 54-55 0.8601973055853946 1102 56-57 0.7903510308986625 1102 58-59 0.6375657764298737 1102 60-61 0.7392180562265409 1102 62-63 0.739859682755629 1102 64-65 0.8719572126026733 1102 66-67 0.9166581905611011 1102 68-69 1.2575581664367022 1102 70-71 1.2097493249826137 1102 72-73 1.2562646504245478 1102 74-75 1.2819347325549693 1102 76-77 1.4269589583400624 1102 78-79 1.7066127829235498 1102 80-81 1.8487624990074316 1102 82-83 1.916885643155961 1102 84-85 2.2479215941051685 1102 86-87 2.401077292499597 1102 88-89 2.7105855176046703 1102 90-91 2.7607186927941036 1102 92-93 3.224117159317842 1102 94-95 3.4474045556480597 1102 96-97 3.597038230328934 1102 98-99 3.8180211651632625 1102 100-101 3.729921745083704 1103 1 0.5647938956566385 1103 2 0.49800891825213967 1103 3 0.43559566473935973 1103 4 0.34192437234589335 1103 5 0.2776475338235329 1103 6 0.4205060789213917 1103 7 0.43551353085666733 1103 8 0.39985944026269493 1103 9 0.5506013662933071 1103 10-11 0.6203199793004401 1103 12-13 0.5357567736684956 1103 14-15 0.7669978509017525 1103 16-17 0.8044458611895777 1103 18-19 0.8077705806760704 1103 20-21 0.8788516983090986 1103 22-23 0.7844095771425614 1103 24-25 0.7510871475212468 1103 26-27 0.8034971691048938 1103 28-29 0.8340814647346164 1103 30-31 0.8782570504054164 1103 32-33 0.9322203582195954 1103 34-35 0.8511655995750544 1103 36-37 0.9854442884808705 1103 38-39 0.9272131695015204 1103 40-41 0.894774498447795 1103 42-43 1.226561735242079 1103 44-45 1.1681862731024424 1103 46-47 1.1114826341618596 1103 48-49 1.0830057344316586 1103 50-51 1.111357444945206 1103 52-53 1.2517613660023343 1103 54-55 1.328438467856408 1103 56-57 1.3119816167083584 1103 58-59 1.4318427826471734 1103 60-61 1.4870330353848047 1103 62-63 1.5806595444510165 1103 64-65 1.7935986834978834 1103 66-67 1.8433657083133994 1103 68-69 1.9365275409284664 1103 70-71 2.18446071014176 1103 72-73 2.267821409742499 1103 74-75 2.193074016828586 1103 76-77 2.3146485669251895 1103 78-79 2.6917200650457573 1103 80-81 2.5334798389486544 1103 82-83 2.761107903796134 1103 84-85 2.8430425145992224 1103 86-87 2.984490983375977 1103 88-89 3.0574362062464076 1103 90-91 3.2335309506809864 1103 92-93 3.4531265525063404 1103 94-95 4.091119699363201 1103 96-97 4.249070859973504 1103 98-99 4.4125113909967695 1103 100-101 4.2678370591184205 1104 1 0.3019309355866433 1104 2 0.16135414403271398 1104 3 0.23984176099077814 1104 4 0.12794007412929176 1104 5 0.14907540155699905 1104 6 0.18202498134468925 1104 7 0.19590065698932335 1104 8 0.22221630281461557 1104 9 0.2831221663477237 1104 10-11 0.3464559191204373 1104 12-13 0.2812894825897061 1104 14-15 0.38304948804840677 1104 16-17 0.47132304997455776 1104 18-19 0.3897938682926849 1104 20-21 0.3874496796424225 1104 22-23 0.2997624450519538 1104 24-25 0.26835641176109704 1104 26-27 0.3738103837952309 1104 28-29 0.33676547513992006 1104 30-31 0.3068994348997265 1104 32-33 0.25603914734796973 1104 34-35 0.2886698510663024 1104 36-37 0.2924471102982338 1104 38-39 0.3574218620125649 1104 40-41 0.5270961527880615 1104 42-43 0.3997968092825275 1104 44-45 0.28302227695868964 1104 46-47 0.39646566338163325 1104 48-49 0.5671743538522307 1104 50-51 0.4226633623173299 1104 52-53 0.4489607767749746 1104 54-55 0.6543355031889391 1104 56-57 0.7786006666730501 1104 58-59 0.8081408069058895 1104 60-61 0.870614303959421 1104 62-63 0.9177623378860105 1104 64-65 1.0528598974657584 1104 66-67 1.0539816526370416 1104 68-69 1.0797893084698273 1104 70-71 1.3924196190390496 1104 72-73 1.471740522336063 1104 74-75 1.7331805328106746 1104 76-77 1.5950171155071295 1104 78-79 1.8282462976263716 1104 80-81 2.0340685971848167 1104 82-83 2.198332436483941 1104 84-85 2.345818005367917 1104 86-87 2.4317971212392315 1104 88-89 2.7328597720571466 1104 90-91 2.879897772864272 1104 92-93 3.142203562552762 1104 94-95 3.3971860202810085 1104 96-97 3.6155840038252016 1104 98-99 3.7642287588956087 1104 100-101 3.700255940665425 1105 1 0.33019843975641194 1105 2 0.306494141903471 1105 3 0.16965119354693314 1105 4 0.13934395645300413 1105 5 0.1676743582268614 1105 6 0.18905503918917788 1105 7 0.2549623696122012 1105 8 0.2612861775440294 1105 9 0.27968520974884115 1105 10-11 0.19889656641097275 1105 12-13 0.242106935302715 1105 14-15 0.44068815514092563 1105 16-17 0.46950077097245924 1105 18-19 0.31243218051100285 1105 20-21 0.4749501232351676 1105 22-23 0.4810037949933985 1105 24-25 0.4898369469113888 1105 26-27 0.41874810337363755 1105 28-29 0.5496642650189116 1105 30-31 0.5497842072479315 1105 32-33 0.593948583452871 1105 34-35 0.6377258857020252 1105 36-37 0.5748222832994117 1105 38-39 0.6411074079865173 1105 40-41 0.7387768369855152 1105 42-43 0.7754870395958804 1105 44-45 0.8405198815578601 1105 46-47 0.7851717920590104 1105 48-49 0.8848364426417561 1105 50-51 0.8084400044976761 1105 52-53 0.8202461311168534 1105 54-55 0.9428961334455153 1105 56-57 1.0207322185379084 1105 58-59 1.1440948861847886 1105 60-61 1.1619367426549019 1105 62-63 1.1415016473008208 1105 64-65 1.126435077564416 1105 66-67 1.275228858002734 1105 68-69 1.2654967501817396 1105 70-71 1.3749048030413213 1105 72-73 1.4680587025411818 1105 74-75 1.3954991033774071 1105 76-77 1.509016015397119 1105 78-79 1.7697630299447624 1105 80-81 1.8783178608208573 1105 82-83 1.9958500914752442 1105 84-85 1.9462287722510574 1105 86-87 2.1069718967812996 1105 88-89 2.4735904580289656 1105 90-91 2.515450844300446 1105 92-93 2.6959785368243807 1105 94-95 3.0090920495936153 1105 96-97 3.1780950936438614 1105 98-99 3.345249199649359 1105 100-101 3.34486895035349 1106 1 0.065726802626628 1106 2 0.04895262091190489 1106 3 -0.043158455742052126 1106 4 0.0779517330634718 1106 5 0.20153355714298016 1106 6 -0.021415197163285882 1106 7 0.0850015798952839 1106 8 0.04231454328468942 1106 9 0.09251122057615646 1106 10-11 0.14483359458152023 1106 12-13 0.1719109125917555 1106 14-15 0.14209323812549712 1106 16-17 0.10417689862690338 1106 18-19 0.2805586121456045 1106 20-21 0.1903860864705038 1106 22-23 0.22316633108925288 1106 24-25 0.23855902465453482 1106 26-27 0.0896613068480221 1106 28-29 0.05135050812266684 1106 30-31 0.026430264117188074 1106 32-33 0.2361148511276383 1106 34-35 0.29967755888460346 1106 36-37 0.3511698138553001 1106 38-39 -0.021998327737321688 1106 40-41 0.3246952933748375 1106 42-43 0.2034872290452725 1106 44-45 0.2909991024041787 1106 46-47 0.46972649987638704 1106 48-49 0.5327037017683338 1106 50-51 0.6288445650611934 1106 52-53 0.6129069765204136 1106 54-55 0.07116427896561817 1106 56-57 0.5535168662481595 1106 58-59 0.6134264742873654 1106 60-61 0.627298074311895 1106 62-63 0.5514227674634071 1106 64-65 0.8446662804532323 1106 66-67 0.9599777514460683 1106 68-69 0.5944064402310545 1106 70-71 0.8319962382065746 1106 72-73 1.120030124387565 1106 74-75 1.038944010920364 1106 76-77 1.3276536733433737 1106 78-79 1.6491239539985223 1106 80-81 1.6317689972301608 1106 82-83 1.6426547117440329 1106 84-85 1.9094671225487225 1106 86-87 1.6094390445346036 1106 88-89 1.836160425133663 1106 90-91 2.1656067094813736 1106 92-93 2.256848769732315 1106 94-95 2.554534643557645 1106 96-97 2.3413789404998653 1106 98-99 2.713938812655922 1106 100-101 3.1779135168875428 1107 1 0.28166870528197663 1107 2 0.11126153273077222 1107 3 0.03667445446420459 1107 4 0.08535010084624872 1107 5 0.1843081085011633 1107 6 0.2833221008525584 1107 7 0.14006221600237012 1107 8 0.09003864627345592 1107 9 0.21764878227462248 1107 10-11 0.19008594538836832 1107 12-13 0.2103481027374272 1107 14-15 0.30747277221355773 1107 16-17 0.32438062614033925 1107 18-19 0.2527218447637978 1107 20-21 0.254646195212338 1107 22-23 0.3531208676289097 1107 24-25 0.3817971180766264 1107 26-27 0.31535497192751194 1107 28-29 0.30417106774000047 1107 30-31 0.5072726865105963 1107 32-33 0.5541032904969825 1107 34-35 0.4250119291516583 1107 36-37 0.5849632751800371 1107 38-39 0.5827987619474584 1107 40-41 0.5789074053418872 1107 42-43 0.6268777571732329 1107 44-45 0.664803950023952 1107 46-47 0.6349989809991001 1107 48-49 0.7593865852794579 1107 50-51 0.6721085364265704 1107 52-53 0.5560536799428419 1107 54-55 0.4851498091093305 1107 56-57 0.47622925606260225 1107 58-59 0.4031139776923638 1107 60-61 0.5359821672763658 1107 62-63 0.7918637276168212 1107 64-65 0.924794744011642 1107 66-67 1.1166129923016186 1107 68-69 1.097768529861355 1107 70-71 1.3724607609083357 1107 72-73 1.2673619722361558 1107 74-75 1.3482750524667217 1107 76-77 1.4961758793426974 1107 78-79 1.569990701658611 1107 80-81 1.654903333719698 1107 82-83 1.6811721235138712 1107 84-85 2.0004887020294184 1107 86-87 1.9994011265377196 1107 88-89 2.467599674618832 1107 90-91 2.7574521609561415 1107 92-93 2.9504524231531057 1107 94-95 3.1275142571863306 1107 96-97 3.2966023352501814 1107 98-99 3.614266645337313 1107 100-101 3.406927322089281 1108 1 0.18987264455574504 1108 2 0.10396589399694989 1108 3 0.03979440199479001 1108 4 0.06314211451270069 1108 5 0.11453293385731911 1108 6 0.14632073827463898 1108 7 0.24789195477381298 1108 8 0.23791876992231664 1108 9 0.20227148038226517 1108 10-11 0.10439225087020532 1108 12-13 0.1051769161761058 1108 14-15 0.24537417343174184 1108 16-17 0.2750089118342913 1108 18-19 0.24728398950211528 1108 20-21 0.2674340906395756 1108 22-23 0.1474157081361085 1108 24-25 0.19986716373315971 1108 26-27 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2 5332.0 3 1774.0 4 1823.0 5 2068.0 6 2367.0 7 2910.0 8 3586.0 9 4496.0 10 5548.0 11 6944.0 12 9003.0 13 10789.0 14 12641.0 15 15377.0 16 18695.0 17 22950.0 18 22285.0 19 18315.0 20 18483.0 21 19407.0 22 21117.0 23 23568.0 24 26105.0 25 29119.0 26 32871.0 27 37959.0 28 43769.0 29 50791.0 30 60200.0 31 71986.0 32 88231.0 33 111028.0 34 146806.0 35 201998.0 36 257510.0 37 288699.0 38 155616.0 39 9246.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.51944962140287 18.521277164189012 14.306918161597745 22.652355052810375 2 15.613653219825224 19.73192308105726 40.75467844743263 23.899745251684887 3 19.537637500705475 24.300691501226858 29.459188527475956 26.702482470591715 4 12.634320893023295 16.50434293694235 36.767870168250845 34.093466001783504 5 13.524140419132541 37.51191603369878 33.97708142797769 14.986862119190983 6 32.17099423140719 35.873023212550784 17.292121776505066 14.663860779536952 7 28.582101103739472 33.21661370838404 20.058863238639052 18.142421949237445 8 26.694401509956382 34.89334213281339 19.22131050627823 19.190945850951998 9 26.657615793665894 16.818983614910078 20.064398788773907 36.45900180265012 10-11 25.23635187035026 26.757216596108453 26.476623074026868 21.52980845951442 12-13 26.245394977815007 24.126889943564038 27.34441951527044 22.283295563350514 14-15 22.843465374337118 25.45683195640403 26.02644940339035 25.6732532658685 16-17 21.925690809123562 28.680304735022894 26.92556048172232 22.46844397413122 18-19 21.872767779160295 26.6620622562865 29.896427640436755 21.56874232411645 20-21 22.53837208146016 25.823875310791756 30.260037051779232 21.377715555968855 22-23 22.165416257619828 25.84599127826589 30.158350508149717 21.830241955964567 24-25 21.892322757024253 26.05444797128449 30.289630651235537 21.763598620455717 26-27 21.50905180592653 26.480543683517105 30.116935173921856 21.89346933663451 28-29 21.59637875096039 26.71316129139752 30.049766913266257 21.640693044375833 30-31 21.775431266038694 26.395489050353405 29.981814414582438 21.847265269025463 32-33 21.452454394131664 26.396070862497922 29.908989815437387 22.242484927933027 34-35 22.084151113062823 26.573462845096774 29.763348006113493 21.579038035726906 36-37 22.153835156386258 26.19356600223749 29.708565038822794 21.944033802553463 38-39 21.743845255201457 26.541547684427396 29.69358737228105 22.021019688090096 40-41 22.077975115605312 26.455353417352445 29.71602643896408 21.750645028078168 42-43 21.881090544278038 26.406396848110592 29.547270549533337 22.165242058078032 44-45 21.79498614306546 26.731901897846615 29.61660511014087 21.856506848947056 46-47 22.218765205611437 26.868112219058066 28.91889328539007 21.99422928994043 48-49 22.018309431159885 26.98119539257698 28.854777940448272 22.145717235814864 50-51 22.203168714970932 27.2150888732831 28.662352448750305 21.91938996299566 52-53 22.417358757798283 27.00423448550813 28.60335588752527 21.975050869168314 54-55 21.930985902627818 27.203624470549297 28.784628883466844 22.08076074335604 56-57 22.04597487463376 27.403961105705243 28.474940453387077 22.075123566273916 58-59 22.060551972518237 26.993224392402272 28.548086772695434 22.398136862384057 60-61 21.87699644890676 27.129755947637896 28.82954161398773 22.163705989467616 62-63 21.973241990731413 27.02596547786957 28.944912351400365 22.055880179998656 64-65 21.721727342260426 27.106460729020558 28.873789803792192 22.298022124926824 66-67 21.727101361637587 27.278715423466416 28.61472509222961 22.37945812266639 68-69 22.158997951576616 26.969099029517444 28.744121696497533 22.127781322408406 70-71 21.910418906077595 27.090063879798404 28.512334413444712 22.487182800679285 72-73 21.951022278890868 27.17924208194942 28.411838839317255 22.457896799842462 74-75 21.9703767303613 27.491387147453327 28.196265578893296 22.341970543292074 76-77 22.105060407195513 27.201169540686106 28.27590801525969 22.41786203685869 78-79 22.142484682395615 27.30266436453736 28.24669140551227 22.308159547554755 80-81 22.23088491861573 27.419108714212868 28.058539290283374 22.291467076888026 82-83 22.284396825307276 27.470177816857422 27.98155340510054 22.263871952734764 84-85 22.180134510535584 27.499251152151103 27.978836149404213 22.341778187909096 86-87 22.397829532328686 27.400273994681278 27.8360598490343 22.365836623955733 88-89 22.31909719889837 27.37018875528985 27.72220057768523 22.588513468126553 90-91 22.32539980454806 27.436760925518943 27.75350377294256 22.484335496990433 92-93 22.43034242041283 27.079437796060834 27.93890078147164 22.551319002054697 94-95 22.276231265109434 26.967252516996055 27.970826518386318 22.785689699508197 96-97 22.61440416225687 26.56606553716763 27.916047683033092 22.903482617542405 98-99 22.41058798400303 26.733477433230657 27.632789756995983 23.22314482577033 100-101 23.27869108239218 25.84703917945429 27.752573015424236 23.12169672272929 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2788.0 1 1790.0 2 1264.5 3 1626.5 4 1132.5 5 933.5 6 1806.0 7 3729.5 8 5771.5 9 6223.5 10 4626.0 11 3059.5 12 2699.0 13 2673.0 14 2718.0 15 2799.5 16 2938.5 17 3099.0 18 3297.0 19 3599.0 20 3963.0 21 4518.0 22 5320.0 23 6380.5 24 7808.5 25 9539.0 26 11824.0 27 14517.0 28 16881.5 29 19137.5 30 21719.0 31 25175.5 32 30183.5 33 33257.5 34 34407.5 35 36879.5 36 40470.5 37 44048.5 38 46876.5 39 49419.0 40 52015.5 41 54903.5 42 56787.0 43 58275.5 44 60167.0 45 63612.0 46 65858.5 47 66059.0 48 67248.0 49 67102.0 50 65513.5 51 63830.5 52 62849.0 53 62903.0 54 62696.5 55 59592.0 56 55031.5 57 50622.0 58 46444.0 59 41257.0 60 34539.0 61 29045.0 62 24081.5 63 18113.0 64 12949.0 65 9874.5 66 7621.5 67 5899.0 68 4502.0 69 3319.0 70 2432.5 71 1758.0 72 1343.5 73 1069.5 74 806.5 75 604.5 76 431.0 77 263.0 78 156.5 79 110.0 80 68.5 81 42.5 82 26.5 83 19.5 84 15.5 85 12.0 86 9.0 87 4.5 88 4.5 89 6.0 90 4.5 91 1.5 92 2.0 93 3.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4648081860393153 2 0.04045313963102224 3 0.052003504864315445 4 0.055656643635775625 5 0.08187328658390158 6 0.08133606029398097 7 0.044536059434418906 8 0.0374446724074668 9 0.043998833144498296 10-11 0.04794744637541481 12-13 0.04343474554008165 14-15 0.0370954753190184 16-17 0.038384818414827875 18-19 0.04716846825502991 20-21 0.056059563353216074 22-23 0.047732555859446565 24-25 0.06626686286170774 26-27 0.057268322505537456 28-29 0.04510014703883555 30-31 0.04692671642456564 32-33 0.045771679901236324 34-35 0.05041868730904963 36-37 0.045315037554803796 38-39 0.026377810835102152 40-41 0.02404087647394748 42-43 0.02331562098255465 44-45 0.013484379877007414 46-47 0.02490043853782046 48-49 0.024685548021852216 50-51 0.015042336117777194 52-53 0.014290219311888333 54-55 0.015364671891729563 56-57 0.014290219311888333 58-59 0.013511241191503445 60-61 0.01590189818165018 62-63 0.015472117149713686 64-65 0.016089927383122392 66-67 0.008676204582217918 68-69 0.012114452837709847 70-71 0.01823883254280485 72-73 0.016197372641106513 74-75 0.012947153587086797 76-77 0.009105985614154408 78-79 0.012759124385614584 80-81 0.016331679213586667 82-83 0.01377985433646375 84-85 0.01184583969274954 86-87 0.0033845256264998687 88-89 0.02890277439772904 90-91 0.03209927082275669 92-93 0.016600292358546972 94-95 0.03295883288662967 96-97 0.02683445318153467 98-99 0.023396204926042743 100-101 0.1476029231556887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1861413.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.57508856848503 #Duplication Level Percentage of deduplicated Percentage of total 1 80.1770024662262 49.36906028013521 2 12.044440949037748 14.832750363897743 3 3.5072749969654695 6.478823057165456 4 1.4813024803546901 3.648453256982264 5 0.7551578013411717 2.3249454250382535 6 0.455995266709487 1.6846769360667968 7 0.2866395356504403 1.23548983564337 8 0.20442061704322728 1.0069774079728864 9 0.1574141429738701 0.8723510815993395 >10 0.8491740552003693 9.542319682037856 >50 0.051627483940995374 2.1577402225599385 >100 0.025616900150807177 2.969324007576734 >500 0.0022725758786317834 1.020711333448674 >1k 0.001398508233004174 1.3519244916898097 >5k 1.7481352912552175E-4 0.8572575860358808 >10k+ 8.740676456276088E-5 0.6471950321497656 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12025 0.6460146136295384 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9713 0.5218078953998925 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6215 0.3338861391856616 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2549 0.13693898130076454 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2504 0.13452146299612178 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1995 0.1071766448391625 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1887 0.10137460090801986 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.372262899206141E-5 0.0 0.0 0.0 5.372262899206141E-5 7 5.372262899206141E-5 0.0 0.0 0.0 5.372262899206141E-5 8 5.372262899206141E-5 0.0 0.0 0.0 5.372262899206141E-5 9 5.372262899206141E-5 0.0 0.0 0.0 5.372262899206141E-5 10-11 5.372262899206141E-5 0.0 0.0 0.0 5.372262899206141E-5 12-13 5.372262899206141E-5 0.0 0.0 0.0 1.343065724801535E-4 14-15 5.372262899206141E-5 0.0 0.0 0.0 1.6116788697618422E-4 16-17 5.372262899206141E-5 0.0 0.0 1.0744525798412282E-4 2.6861314496030706E-4 18-19 8.058394348809211E-5 0.0 0.0 1.0744525798412282E-4 2.6861314496030706E-4 20-21 1.0744525798412282E-4 0.0 0.0 2.1489051596824564E-4 2.6861314496030706E-4 22-23 1.6116788697618422E-4 0.0 0.0 3.491970884483992E-4 2.6861314496030706E-4 24-25 2.1489051596824564E-4 0.0 0.0 8.864233783690132E-4 2.6861314496030706E-4 26-27 2.1489051596824564E-4 0.0 0.0 0.001826569385730088 2.6861314496030706E-4 28-29 2.4175183046427635E-4 0.0 0.0 0.004673868722309343 2.6861314496030706E-4 30-31 3.2233577395236843E-4 0.0 0.0 0.015096058746769257 2.954744594563378E-4 32-33 3.2233577395236843E-4 0.0 0.0 0.03303941683011777 3.2233577395236843E-4 34-35 4.0291971744046057E-4 0.0 0.0 0.06137810362343016 3.4919708844839914E-4 36-37 4.297810319364913E-4 0.0 0.0 0.10500087836498402 3.7605840294442985E-4 38-39 4.8350366092855265E-4 0.0 0.0 0.1726108069514933 3.7605840294442985E-4 40-41 4.8350366092855265E-4 0.0 0.0 0.24540496923573651 3.7605840294442985E-4 42-43 5.640876044166448E-4 0.0 0.0 0.3145459927485195 4.297810319364913E-4 44-45 5.909489189126754E-4 0.0 0.0 0.39846073923411945 4.8350366092855265E-4 46-47 6.446715479047369E-4 0.0 0.0 0.5000233693436116 4.8350366092855265E-4 48-49 6.446715479047369E-4 0.0 0.0 0.6073074594407581 4.8350366092855265E-4 50-51 6.446715479047369E-4 0.0 0.0 0.7202324255820712 4.8350366092855265E-4 52-53 6.446715479047369E-4 0.0 0.0 0.822278559352492 5.103649754245834E-4 54-55 6.446715479047369E-4 0.0 0.0 0.9375941824839517 5.909489189126754E-4 56-57 6.446715479047369E-4 0.0 0.0 1.0680058643621808 6.178102334087061E-4 58-59 6.715328624007676E-4 0.0 0.0 1.2128420721247783 6.983941768967983E-4 60-61 6.983941768967983E-4 0.0 0.0 1.3688525867177246 6.983941768967983E-4 62-63 6.983941768967983E-4 0.0 0.0 1.5176374077112387 6.983941768967983E-4 64-65 6.983941768967983E-4 0.0 0.0 1.6643539074885585 6.983941768967983E-4 66-67 7.521168058888597E-4 0.0 0.0 1.8339293859020003 7.252554913928289E-4 68-69 7.521168058888597E-4 0.0 0.0 2.022656981551112 7.521168058888597E-4 70-71 7.789781203848905E-4 0.0 0.0 2.2148228254557156 7.521168058888597E-4 72-73 8.058394348809211E-4 0.0 0.0 2.4152082315961048 7.789781203848905E-4 74-75 8.058394348809211E-4 0.0 0.0 2.606245900291875 8.058394348809211E-4 76-77 8.058394348809211E-4 0.0 0.0 2.809129408680395 8.864233783690132E-4 78-79 8.058394348809211E-4 0.0 0.0 3.040029268088275 9.132846928650439E-4 80-81 8.595620638729826E-4 0.0 0.0 3.2857297117834676 9.401460073610746E-4 82-83 9.670073218571053E-4 0.0 0.0 3.538628987763597 9.670073218571053E-4 84-85 0.0010207299508491667 0.0 0.0 3.7958797966920828 9.670073218571053E-4 86-87 0.0010744525798412282 0.0 0.0 4.055682430497692 9.670073218571053E-4 88-89 0.0010744525798412282 0.0 0.0 4.329614115728213 9.670073218571053E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4745 0.0 57.466602 1 GTATCAA 8740 0.0 43.328873 1 ATCAACG 11025 0.0 34.09716 3 TATCAAC 11170 0.0 33.909817 2 TCAACGC 11145 0.0 33.68739 4 CAACGCA 11330 0.0 33.095387 5 AACGCAG 11730 0.0 32.003025 6 TGGTATC 1885 0.0 30.758757 2 GTGGTAT 2070 0.0 29.298744 1 ACGCAGA 13055 0.0 28.634949 7 CGCAGAG 13040 0.0 28.449324 8 GCAGAGT 14025 0.0 25.942556 9 GAGTACT 8880 0.0 24.498825 12-13 GTACATG 8845 0.0 24.079788 1 TACATGG 8680 0.0 23.871904 2 GTACTTT 9395 0.0 23.133715 14-15 CAGAGTA 13525 0.0 22.950352 10-11 ACATGGG 8550 0.0 22.678501 3 TAGCGCG 300 7.2759576E-12 22.178242 4 AGAGTAC 13330 0.0 21.148111 10-11 >>END_MODULE