FastQCFastQC Report
Thu 26 May 2016
SRR938162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938162_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861413
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117850.6331211826714437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103230.55457869908505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65560.3522055556719546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24970.13414540459317734No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24650.13242628046543137No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21530.1156648202199082No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18750.10072992936011513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48800.056.8414541
GTATCAA81800.039.7749251
ATCAACG105950.030.4381053
TATCAAC106950.030.155122
TCAACGC107700.030.0309124
CAACGCA109850.029.5296135
AACGCAG113300.028.7142726
TGGTATC20200.027.0407412
GTGGTAT21600.026.3875851
TAGGACG1109.488108E-525.905724
ACGCAGA128300.025.098067
CGCAGAG129200.024.8129488
TAAGGTG10900.024.4004965
GTACATG82200.023.459911
TACATGG81600.022.8174972
GTAAGGT10050.022.6836154
GAGTACT90200.022.53587212-13
GCAGAGT141450.022.496199
TACCTGG13450.022.247972
AGGTAAG9800.021.8101462
Produced by FastQC (version 0.11.5)