FastQCFastQC Report
Thu 26 May 2016
SRR938161_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938161_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880377
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123550.6570490917512818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101210.5382431289044697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64270.3417931616904482No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25200.13401567877079967No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24460.13008029772753016No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20640.10976522261227403No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA19900.10582984156900452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47050.053.87361
GTATCAA83650.041.372631
ATCAACG106300.032.4553223
TATCAAC106700.032.378192
TCAACGC106650.032.215144
CAACGCA109150.031.5208135
AACGCAG112500.030.5821936
TGGTATC20750.029.542972
GTGGTAT24100.027.1359251
CGCAGAG125950.026.914248
ACGCAGA127000.026.8414657
GTACATG85400.024.4266931
GCAGAGT137200.024.2902249
TACATGG82050.024.0933592
TAAGGTG10750.023.870855
ACATGGG84050.022.8981253
GAGTACT86600.022.83508312-13
GTAAGGT10650.022.7563784
CAGAGTA134500.021.50714710-11
GTACTTT93400.021.23392714-15
Produced by FastQC (version 0.11.5)