##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938161_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1880377 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.870615839270528 33.0 31.0 34.0 30.0 34.0 2 32.05465978364977 34.0 31.0 34.0 30.0 34.0 3 32.11791412041309 34.0 31.0 34.0 30.0 34.0 4 35.63821829345924 37.0 35.0 37.0 33.0 37.0 5 35.665630349658606 37.0 35.0 37.0 35.0 37.0 6 35.69381937770989 37.0 35.0 37.0 35.0 37.0 7 35.63632133343473 37.0 35.0 37.0 33.0 37.0 8 35.6221608751862 37.0 35.0 37.0 33.0 37.0 9 37.277570933913786 39.0 37.0 39.0 34.0 39.0 10-11 37.19895185912186 39.0 37.0 39.0 34.0 39.0 12-13 37.09879641157066 39.0 37.0 39.0 33.5 39.0 14-15 38.33721641989877 40.0 38.0 41.0 33.5 41.0 16-17 38.2567312299608 40.0 38.0 41.0 33.0 41.0 18-19 38.21515898141702 40.0 38.0 41.0 33.5 41.0 20-21 38.11409626899287 40.0 38.0 41.0 33.0 41.0 22-23 37.9673919113029 40.0 38.0 41.0 33.0 41.0 24-25 37.907848266597604 40.0 38.0 41.0 33.0 41.0 26-27 37.90846702549542 40.0 38.0 41.0 33.0 41.0 28-29 37.81495811744134 40.0 38.0 41.0 33.0 41.0 30-31 37.59959571936904 40.0 37.0 41.0 32.5 41.0 32-33 37.357208155598585 40.0 37.0 41.0 32.0 41.0 34-35 37.14515918882224 40.0 36.0 41.0 31.0 41.0 36-37 36.926567119253214 40.0 36.0 41.0 31.0 41.0 38-39 36.68415562411155 39.5 35.5 41.0 30.0 41.0 40-41 36.44515115851768 39.0 35.0 41.0 30.0 41.0 42-43 36.15406272252851 39.0 35.0 41.0 29.5 41.0 44-45 35.90030669381725 39.0 35.0 41.0 28.5 41.0 46-47 35.72485038904433 39.0 35.0 41.0 27.5 41.0 48-49 35.44988824049645 39.0 35.0 41.0 26.5 41.0 50-51 34.79988534214149 38.5 34.5 40.0 25.0 40.5 52-53 34.877735954013474 38.5 34.5 40.0 26.0 41.0 54-55 35.01912674958267 39.0 35.0 40.0 25.5 41.0 56-57 34.81777271259965 38.5 35.0 40.0 25.0 41.0 58-59 34.56108296368228 38.0 34.0 40.0 24.0 41.0 60-61 34.189668880229874 38.0 34.0 40.0 23.0 41.0 62-63 33.77419155839494 37.0 34.0 40.0 22.0 41.0 64-65 33.35904076682495 36.5 33.0 40.0 20.5 41.0 66-67 32.935727516343796 36.0 33.0 39.0 19.5 41.0 68-69 32.459725363584 35.5 32.0 39.0 17.0 40.5 70-71 31.98132555333319 35.0 32.0 38.5 13.0 40.0 72-73 31.505028246995153 35.0 31.0 37.5 8.0 40.0 74-75 30.988888930251754 35.0 31.0 37.0 7.0 39.0 76-77 30.466736989444136 35.0 30.5 36.5 2.0 39.0 78-79 29.909698959304436 34.0 30.0 36.0 2.0 38.5 80-81 29.391598067834266 34.0 29.0 35.0 2.0 37.0 82-83 28.861470598715044 34.0 29.0 35.0 2.0 37.0 84-85 28.375488798256946 34.0 28.5 35.0 2.0 36.0 86-87 27.887459004231598 34.0 27.0 35.0 2.0 36.0 88-89 27.343254836663075 33.0 26.0 35.0 2.0 35.5 90-91 26.74516174150184 33.0 25.0 35.0 2.0 35.0 92-93 26.152431134820304 32.5 24.0 35.0 2.0 35.0 94-95 25.573717929968296 32.0 21.5 35.0 2.0 35.0 96-97 24.755567899415915 32.0 14.5 34.0 2.0 35.0 98-99 23.706221146078686 31.5 2.0 34.0 2.0 35.0 100-101 20.895407144418378 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4627526058559752 1101 2 0.41550275885543186 1101 3 0.47559284585649664 1101 4 0.2941352737792471 1101 5 0.3795115468969925 1101 6 0.38627131480581767 1101 7 0.42100279663667806 1101 8 0.35640713259267187 1101 9 0.37296364599706777 1101 10-11 0.4217780441357135 1101 12-13 0.46051953501851983 1101 14-15 0.5603345911845352 1101 16-17 0.5461153827589058 1101 18-19 0.5909429689853951 1101 20-21 0.639864308254559 1101 22-23 0.6129708672972285 1101 24-25 0.5530783159684205 1101 26-27 0.6842674241067002 1101 28-29 0.6313554608345129 1101 30-31 0.5594305255061442 1101 32-33 0.49425432208870035 1101 34-35 0.6420867630954561 1101 36-37 0.5610950177440515 1101 38-39 0.7461948119995085 1101 40-41 0.759995094750856 1101 42-43 0.7849921455065783 1101 44-45 0.647030697765409 1101 46-47 0.6470084180128453 1101 48-49 0.8317385614479207 1101 50-51 0.9084332234992232 1101 52-53 0.8763201531165592 1101 54-55 0.83577315815171 1101 56-57 0.6894290140683452 1101 58-59 0.7034397008604074 1101 60-61 0.7416402797708699 1101 62-63 0.6772340460119253 1101 64-65 0.7130370262512642 1101 66-67 0.7375435173549079 1101 68-69 0.6949895652298537 1101 70-71 0.8803158375302758 1101 72-73 0.9312888513371576 1101 74-75 0.863702045035069 1101 76-77 0.9211122085514702 1101 78-79 0.7577349037171146 1101 80-81 0.7794197515901011 1101 82-83 0.6286835775628994 1101 84-85 0.6572580975060696 1101 86-87 0.6368836805679869 1101 88-89 0.6382471448901832 1101 90-91 0.8251383140495356 1101 92-93 0.9338205928803767 1101 94-95 1.0914114706404199 1101 96-97 0.966349467031911 1101 98-99 1.1537718407008875 1101 100-101 1.0392879331453528 1102 1 0.5356603607995112 1102 2 0.4193192516866162 1102 3 0.43816057495224214 1102 4 0.3210782569539177 1102 5 0.38165946178131804 1102 6 0.3907324854801075 1102 7 0.2936053535048515 1102 8 0.4056772055853699 1102 9 0.4551517281032389 1102 10-11 0.5096741797091866 1102 12-13 0.4361476787710572 1102 14-15 0.4680629891676773 1102 16-17 0.4921361277334597 1102 18-19 0.6046260665941716 1102 20-21 0.4888625547308294 1102 22-23 0.5700944816190869 1102 24-25 0.379842002858247 1102 26-27 0.38477740220282186 1102 28-29 0.5468799100520982 1102 30-31 0.3855290565507943 1102 32-33 0.4560325104377867 1102 34-35 0.40259804571615376 1102 36-37 0.41435041331837397 1102 38-39 0.3411285542861222 1102 40-41 0.47980976124762975 1102 42-43 0.6319425917753421 1102 44-45 0.6331602643598728 1102 46-47 0.6378046937468653 1102 48-49 0.5206048992530512 1102 50-51 0.4966554902168596 1102 52-53 0.5051176848347154 1102 54-55 0.578476163412283 1102 56-57 0.45956723299790525 1102 58-59 0.6196558514814186 1102 60-61 0.47241902026755866 1102 62-63 0.5844284990520734 1102 64-65 0.5916275026151041 1102 66-67 0.47191880177160783 1102 68-69 0.4816747682806195 1102 70-71 0.5716903513673373 1102 72-73 0.4657639470528494 1102 74-75 0.42628888693275613 1102 76-77 0.36419929011762875 1102 78-79 0.3092560400490356 1102 80-81 0.5596217708727913 1102 82-83 0.6951961283028361 1102 84-85 0.6833379708154972 1102 86-87 0.5544475871033505 1102 88-89 0.5879486211265963 1102 90-91 0.7836180271754785 1102 92-93 0.991305378483279 1102 94-95 1.1037528798910188 1102 96-97 1.1791356281497976 1102 98-99 1.2468587705781395 1102 100-101 1.347858811302757 1103 1 0.43081610435354634 1103 2 0.37171279325118434 1103 3 0.3020558403138125 1103 4 0.22310554539114946 1103 5 0.25418660835994444 1103 6 0.13267018805849773 1103 7 0.2242493915745385 1103 8 0.2704236225808927 1103 9 0.18675954494228364 1103 10-11 0.2684081561348606 1103 12-13 0.22659242699351978 1103 14-15 0.3368910651351129 1103 16-17 0.2989902626968757 1103 18-19 0.391259976819164 1103 20-21 0.3516251006199411 1103 22-23 0.30316602134379167 1103 24-25 0.3484074254679328 1103 26-27 0.30228877238500473 1103 28-29 0.505111148176816 1103 30-31 0.34307249537535256 1103 32-33 0.48362938267046474 1103 34-35 0.46969824087921097 1103 36-37 0.41459436713265063 1103 38-39 0.4153302772233687 1103 40-41 0.36259049083074046 1103 42-43 0.468683755703708 1103 44-45 0.3219794419238582 1103 46-47 0.3646570232371218 1103 48-49 0.39314386738214324 1103 50-51 0.43204211939846004 1103 52-53 0.3499340424215731 1103 54-55 0.24492144467919985 1103 56-57 0.13919461772703556 1103 58-59 0.2047409236573614 1103 60-61 0.16700281571604592 1103 62-63 0.15778766784661258 1103 64-65 0.16602374379193208 1103 66-67 0.32032353726978613 1103 68-69 0.2617767345268689 1103 70-71 0.18566406883658004 1103 72-73 0.43014991422762705 1103 74-75 0.09974327294291996 1103 76-77 0.19154662016671864 1103 78-79 0.3677447730367511 1103 80-81 0.36658552384967535 1103 82-83 0.23434587940546336 1103 84-85 0.4861491355176355 1103 86-87 0.42027083987819225 1103 88-89 0.5318435425885149 1103 90-91 0.7384425093915681 1103 92-93 0.6753174837509626 1103 94-95 0.6884843802392169 1103 96-97 0.6383330093539605 1103 98-99 0.8822359425193689 1103 100-101 0.7490392468516625 1104 1 0.18495854519090216 1104 2 0.14091496822954497 1104 3 0.07840425785013139 1104 4 0.04130406211007198 1104 5 0.13566222029886177 1104 6 0.07493277157936973 1104 7 -0.02189039724259345 1104 8 0.058740655643326534 1104 9 -0.02534408496011764 1104 10-11 0.0708772298651823 1104 12-13 0.012464587015728057 1104 14-15 0.08493959120582417 1104 16-17 0.26287676615302047 1104 18-19 0.3193185652820745 1104 20-21 0.20559036022764587 1104 22-23 0.20251578701273587 1104 24-25 0.15624456085705418 1104 26-27 0.25967544645265406 1104 28-29 0.1864450419471808 1104 30-31 0.2664412783621941 1104 32-33 0.27133871696378264 1104 34-35 0.31582643213572226 1104 36-37 0.2603769778977352 1104 38-39 0.46674652037795994 1104 40-41 0.261573391467806 1104 42-43 0.38799640917865474 1104 44-45 0.35161439361968405 1104 46-47 0.3821033074256306 1104 48-49 0.396535483485458 1104 50-51 0.4766706052866496 1104 52-53 0.5670529469146501 1104 54-55 0.6570035554084228 1104 56-57 0.6350380989259179 1104 58-59 0.578762421378407 1104 60-61 0.6204783297795942 1104 62-63 0.579282070515255 1104 64-65 0.4863312735584415 1104 66-67 0.7307333870176933 1104 68-69 0.779138718527598 1104 70-71 0.9071273659090977 1104 72-73 0.8176589392079734 1104 74-75 0.6824640472577421 1104 76-77 0.6411332643354584 1104 78-79 0.6783981715577561 1104 80-81 0.7429979290831916 1104 82-83 0.8851012627182477 1104 84-85 0.7232217101023544 1104 86-87 0.7471737280730153 1104 88-89 0.7133084954977242 1104 90-91 0.7005270334860043 1104 92-93 0.7922406710287753 1104 94-95 0.8350264301114088 1104 96-97 0.7062972285331099 1104 98-99 0.5513008908699994 1104 100-101 0.5312479519940752 1105 1 0.3324372997007572 1105 2 0.322575206491706 1105 3 0.28272737006100357 1105 4 0.18357342732242898 1105 5 0.13163205035937153 1105 6 0.2602906655705368 1105 7 0.20460307705152303 1105 8 0.2238964029789372 1105 9 0.29048609523876934 1105 10-11 0.22878346513257242 1105 12-13 0.1201559857372132 1105 14-15 0.3182399886351135 1105 16-17 0.3215594175217902 1105 18-19 0.3173204266288394 1105 20-21 0.4638424530213996 1105 22-23 0.3956012013333847 1105 24-25 0.3767353750374909 1105 26-27 0.35199791012342274 1105 28-29 0.4185304953512201 1105 30-31 0.4494192072352945 1105 32-33 0.4313850735362692 1105 34-35 0.4447013371752817 1105 36-37 0.5179819046041487 1105 38-39 0.4185188302799858 1105 40-41 0.4347212354077925 1105 42-43 0.39363617384775296 1105 44-45 0.4848957989609133 1105 46-47 0.405269685445127 1105 48-49 0.35358710532428717 1105 50-51 0.24681473752531957 1105 52-53 0.24640085265527034 1105 54-55 0.34182160998350497 1105 56-57 0.34672354597891086 1105 58-59 0.3239792853755219 1105 60-61 0.26677526954963326 1105 62-63 0.3100290702538899 1105 64-65 0.23496182798084675 1105 66-67 0.2991230716879585 1105 68-69 0.36707544495114774 1105 70-71 0.19859805580433232 1105 72-73 0.28356936250558107 1105 74-75 0.24208347878973413 1105 76-77 0.38977023087762674 1105 78-79 0.4275604739118002 1105 80-81 0.42721416041781524 1105 82-83 0.471094048167501 1105 84-85 0.49856149138189565 1105 86-87 0.6271400674976562 1105 88-89 0.3861065735716984 1105 90-91 0.1576566694485919 1105 92-93 0.5489130923184753 1105 94-95 0.7496090560759754 1105 96-97 0.8903478134405667 1105 98-99 0.7847948732482735 1105 100-101 0.5779887936550203 1106 1 0.153789231031233 1106 2 0.06077263138440969 1106 3 0.1137359537519913 1106 4 0.00116999384603389 1106 5 0.13860629928640833 1106 6 0.08968980720143804 1106 7 0.2727361242618187 1106 8 0.16542000812915347 1106 9 0.13866206090400368 1106 10-11 -0.379049152893181 1106 12-13 -0.07109937906965058 1106 14-15 0.054023250437694514 1106 16-17 0.28749289391664234 1106 18-19 0.3784792249121054 1106 20-21 0.19560210967376435 1106 22-23 0.08256472064667975 1106 24-25 0.1852117698872675 1106 26-27 0.17495928351397794 1106 28-29 0.04433521638127047 1106 30-31 0.1921767179649123 1106 32-33 0.21384215508133053 1106 34-35 0.04931507108086208 1106 36-37 -0.062221958056795756 1106 38-39 0.014280632855090403 1106 40-41 0.26707102081552136 1106 42-43 0.20050312663744307 1106 44-45 0.33119408222271574 1106 46-47 0.5106881403807435 1106 48-49 0.599027019487373 1106 50-51 0.479110874864368 1106 52-53 0.5183965608097765 1106 54-55 0.2699331979663313 1106 56-57 0.40600466185873785 1106 58-59 0.44629688194203965 1106 60-61 0.08034823092302901 1106 62-63 0.2668423749749209 1106 64-65 0.3157021713284891 1106 66-67 3.033291986938025E-4 1106 68-69 -0.12031725891151268 1106 70-71 0.11276114593308506 1106 72-73 0.2859835255957144 1106 74-75 0.26327446591420056 1106 76-77 0.38118653989050344 1106 78-79 0.5957471691907692 1106 80-81 0.6302721003319789 1106 82-83 0.63150177348939 1106 84-85 0.5497953969612901 1106 86-87 0.6909812692143973 1106 88-89 0.571728891168263 1106 90-91 0.32745280678764743 1106 92-93 0.24580150433564185 1106 94-95 0.17423351959528688 1106 96-97 0.45070189069378586 1106 98-99 0.4165008818319649 1106 100-101 0.16704458764643704 1107 1 0.19096739775597626 1107 2 0.1207162154503294 1107 3 -0.004266088513645627 1107 4 0.09609619880481546 1107 5 0.15694809427469636 1107 6 0.09113108213979615 1107 7 0.030754112078803075 1107 8 0.06401247688866363 1107 9 0.06317542384945796 1107 10-11 0.08672442689412918 1107 12-13 0.04519287223047286 1107 14-15 0.08576465682208578 1107 16-17 0.1215428213566625 1107 18-19 0.23267336806477346 1107 20-21 0.2687641808751664 1107 22-23 0.28852120009832305 1107 24-25 0.3132045932864287 1107 26-27 0.2059315320793189 1107 28-29 0.2578173123721683 1107 30-31 0.23891725318847534 1107 32-33 0.32563745213614226 1107 34-35 0.4475973955584962 1107 36-37 0.4131523102783774 1107 38-39 0.4383493178212845 1107 40-41 0.4983989566275113 1107 42-43 0.47296997841660016 1107 44-45 0.398564312341513 1107 46-47 0.4742209621919713 1107 48-49 0.39882130423125517 1107 50-51 0.5162543054295483 1107 52-53 0.545746599846602 1107 54-55 0.5330789045770103 1107 56-57 0.4999463999458911 1107 58-59 0.5248772426687864 1107 60-61 0.517923492602101 1107 62-63 0.403766250956906 1107 64-65 0.3188275986383857 1107 66-67 0.4104113862233305 1107 68-69 0.30158234586817656 1107 70-71 0.41712130587380614 1107 72-73 0.5276217600725062 1107 74-75 0.4892072696405627 1107 76-77 0.6207502537263032 1107 78-79 0.38103469637319165 1107 80-81 0.4414190442115782 1107 82-83 0.3862702762837209 1107 84-85 0.3713813838431541 1107 86-87 0.5405510922033372 1107 88-89 0.3214146116292973 1107 90-91 0.36238525322779935 1107 92-93 0.4727171208307972 1107 94-95 0.60344811848638 1107 96-97 0.7953910075793083 1107 98-99 0.5938275980187946 1107 100-101 0.6966152597415025 1108 1 0.39395884293232086 1108 2 0.27619894670304745 1108 3 0.24595976029383593 1108 4 0.14101908103791772 1108 5 0.17290015215051113 1108 6 0.2641221249943797 1108 7 0.26633544873799764 1108 8 0.2288016419978547 1108 9 0.331803835812309 1108 10-11 0.39971338499048414 1108 12-13 0.2691946570761061 1108 14-15 0.34450326837310996 1108 16-17 0.3780064862153196 1108 18-19 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-0.7796693627912177 2316 60-61 -0.6777625011869972 2316 62-63 -0.5446300204525798 2316 64-65 -0.44043303250296617 2316 66-67 -0.5692718859730803 2316 68-69 -0.5413958681538702 2316 70-71 -0.5577593629189543 2316 72-73 -0.5217732897372471 2316 74-75 -0.4837959528037352 2316 76-77 -0.4545531927562294 2316 78-79 -0.5331905143538265 2316 80-81 -0.4635263211176195 2316 82-83 -0.5068965363401325 2316 84-85 -0.7045525037431979 2316 86-87 -0.8196496071102004 2316 88-89 -0.8846412096285903 2316 90-91 -0.7611335944633524 2316 92-93 -0.7973488609259043 2316 94-95 -0.806601727564729 2316 96-97 -1.0532099383598883 2316 98-99 -1.1778543027066952 2316 100-101 -1.096474525063094 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5240.0 3 1581.0 4 1547.0 5 1677.0 6 1990.0 7 2425.0 8 3069.0 9 3755.0 10 4493.0 11 5765.0 12 7300.0 13 8888.0 14 10589.0 15 12842.0 16 15761.0 17 20785.0 18 19072.0 19 14703.0 20 14013.0 21 14238.0 22 15074.0 23 16715.0 24 18434.0 25 20693.0 26 23437.0 27 27132.0 28 31538.0 29 37450.0 30 44222.0 31 54334.0 32 68481.0 33 88170.0 34 123084.0 35 185629.0 36 269374.0 37 367197.0 38 284455.0 39 35220.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.55849906143236 18.44215943249796 14.316096328254948 22.68324517781474 2 15.627725229311668 19.6720386883463 40.72396456583356 23.976271516508472 3 19.58093569778245 24.291085638025585 29.42607868324179 26.701899980950174 4 12.647256506905629 16.543497245251633 36.74668820867652 34.06255803916623 5 13.496424106266078 37.47063998837575 34.01056295111162 15.02237295424655 6 32.21035516520445 35.8250958708072 17.238484189768563 14.726064774219791 7 28.626638723527904 33.145827634543764 20.054186840825224 18.173346801103108 8 26.702941311059153 34.83765269777148 19.201581810035936 19.257824181133426 9 26.64139936106316 16.780924691865152 20.11879500173462 36.45888094533706 10-11 25.241965077622286 26.746005789971306 26.47808855904544 21.53394057336097 12-13 26.224297811580637 24.097581803526726 27.312651263720877 22.365469121171756 14-15 22.859599678712808 25.438198109896927 26.062840433125782 25.63936177826448 16-17 21.940773457143237 28.68256861206082 26.91681411220051 22.45984381859543 18-19 21.845654708470068 26.66334440340542 29.88496419565133 21.606036692473186 20-21 22.534088368973997 25.816125049229882 30.25868308727261 21.39110349452351 22-23 22.15068017128939 25.83424287141216 30.134703875510265 21.880373081788182 24-25 21.87596726335033 26.095584182484654 30.276237771063286 21.75221078310173 26-27 21.54491879631867 26.449522165374738 30.135112410076726 21.870446628229868 28-29 21.610810008245924 26.73256841468344 30.023700043185325 21.632921533885312 30-31 21.707641970433492 26.392961642730405 29.995534901741227 21.903861485094875 32-33 21.460056247300795 26.44078160810655 29.882888638699832 22.216273505892822 34-35 22.070834463153407 26.62052122247818 29.745317488976102 21.563326825392306 36-37 22.156350498357437 26.192516203468415 29.69147553075373 21.959657767420424 38-39 21.73902865554977 26.545128460774386 29.680022515416837 22.035820368259007 40-41 22.10507134087865 26.430455445702588 29.735532639100416 21.728940574318347 42-43 21.94127240897085 26.352133046992233 29.569054518398158 22.137540025638756 44-45 21.807301911863426 26.705447922440325 29.58776475494509 21.89948541075116 46-47 22.18677262004537 26.91472403881115 28.903690538538125 21.994812802605352 48-49 22.069559486024026 26.988180488989478 28.83955898863479 22.102701036351704 50-51 22.16187821498295 27.252256804991838 28.67419023655891 21.911674743466303 52-53 22.37889664266946 27.04488046124429 28.64985718438734 21.92636571169891 54-55 21.947949323347586 27.206531056910826 28.76003488521472 22.085484734526865 56-57 22.079552820260755 27.459950033792218 28.425309083790612 22.035188062156408 58-59 22.00101119468583 27.022781668386127 28.569893579871213 22.406313557056833 60-61 21.905302991010025 27.137063644243998 28.847416755837607 22.11021660890837 62-63 21.911293642797947 27.11223216441895 28.958849518451814 22.017624674331294 64-65 21.717451788776234 27.128157131340757 28.86332766025127 22.291063419631737 66-67 21.69743428537691 27.30129501117251 28.644762058252365 22.356508645198215 68-69 22.13309794595434 26.969505401932903 28.788287793517252 22.1091088585955 70-71 21.856662317296525 27.138925798681356 28.57498939259117 22.42942249143094 72-73 21.948528961902134 27.192778051763643 28.4277892958244 22.430903690509822 74-75 21.90779646085491 27.506681647174986 28.32563560764687 22.259886284323237 76-77 22.049119678883198 27.2652463723557 28.33192991900269 22.35370402975841 78-79 22.057988536116977 27.425583559409887 28.286292301092747 22.23013560338039 80-81 22.154389596794953 27.519313459955026 28.13892925454619 22.187367688703834 82-83 22.19791308445989 27.601829089739816 28.047425691567042 22.15283213423325 84-85 22.109043636816246 27.633671592506033 28.030928427281015 22.22635634339671 86-87 22.287437210518185 27.504724917648016 27.921943057396014 22.285894814437786 88-89 22.191054127143484 27.531412385553683 27.81123360216813 22.466299885134706 90-91 22.17207246467036 27.60285223087938 27.902793008937564 22.322282295512704 92-93 22.3264862725404 27.290401418071475 28.08083156358127 22.302280745806858 94-95 22.192637643449956 27.146830765301438 28.12304205027516 22.537489540973443 96-97 22.470076661844598 26.82385120291899 28.03190439428949 22.674167740946917 98-99 22.231833449838437 27.008622441542634 27.778008305377426 22.981535803241503 100-101 22.754524829631382 26.475227366796055 28.0219821196184 22.74826568395416 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2837.0 1 1819.5 2 1292.5 3 1641.5 4 1163.0 5 1004.0 6 1874.0 7 3872.0 8 6011.0 9 6572.0 10 4974.0 11 3200.0 12 2765.5 13 2853.0 14 2931.0 15 2937.5 16 3016.5 17 3174.5 18 3416.5 19 3646.0 20 3959.0 21 4620.0 22 5396.5 23 6417.0 24 7881.5 25 9749.0 26 11992.0 27 14590.0 28 17030.5 29 19285.5 30 21797.5 31 25433.5 32 30656.0 33 33578.0 34 34670.0 35 37239.0 36 40889.5 37 44243.0 38 47179.0 39 49937.0 40 52340.0 41 55627.5 42 57767.0 43 59135.0 44 61199.5 45 64684.5 46 66486.0 47 66680.0 48 68357.5 49 68081.0 50 66462.0 51 64981.0 52 63829.5 53 63752.5 54 63945.5 55 60651.5 56 55442.0 57 50776.5 58 46756.5 59 41545.5 60 34452.0 61 28886.0 62 23874.5 63 17658.5 64 12358.0 65 9390.0 66 7267.0 67 5732.5 68 4351.0 69 3113.5 70 2324.0 71 1717.5 72 1236.0 73 981.5 74 767.5 75 559.0 76 386.0 77 243.5 78 158.0 79 94.5 80 47.0 81 32.0 82 22.5 83 12.5 84 13.0 85 15.5 86 10.0 87 6.5 88 8.0 89 8.0 90 6.0 91 4.0 92 6.0 93 4.5 94 1.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5020801679663174 2 0.05041542201377702 3 0.058233003275407 4 0.06690147773558175 5 0.08221755530938743 6 0.10997794591191022 7 0.07035823135466984 8 0.053765813983047014 9 0.05344672903359273 10-11 0.07650061663166483 12-13 0.10822297868991165 14-15 0.05645144564095392 16-17 0.06126431029522271 18-19 0.060413417096677954 20-21 0.07642084539430125 22-23 0.06477424473921985 24-25 0.08974264203401765 26-27 0.06780555175903556 28-29 0.06735351474730865 30-31 0.07227274105139554 32-33 0.07198024651439579 34-35 0.07394793703603054 36-37 0.06934779568139794 38-39 0.04126832012942086 40-41 0.044911206635690606 42-43 0.042571250339692515 44-45 0.02292093553579947 46-47 0.029595129061884932 48-49 0.029861033186430165 50-51 0.02696267822888708 52-53 0.026882906991523507 54-55 0.02645746039225113 56-57 0.026936087816432554 58-59 0.022468898524072568 60-61 0.02980785236152112 62-63 0.02121914913870995 64-65 0.023745238321889708 66-67 0.016805140671259006 68-69 0.018905783255166383 70-71 0.04225216539023824 72-73 0.0381838322846961 74-75 0.031961675770337546 76-77 0.023293201310162805 78-79 0.015874476235350675 80-81 0.018134661293985197 82-83 0.022628440998799706 84-85 0.01909191614234805 86-87 0.00986504302062831 88-89 0.04169376672869324 90-91 0.05349990985850178 92-93 0.03408890876669944 94-95 0.05421785099477392 96-97 0.04382099972505513 98-99 0.03355710051760897 100-101 0.16613689701586437 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1880377.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.31223142029911 #Duplication Level Percentage of deduplicated Percentage of total 1 80.12965298542937 49.12927827470907 2 12.088901872023227 14.823950983895504 3 3.4957583160218277 6.429982285840962 4 1.4444243588005747 3.54243522223592 5 0.7679625934854205 2.3542750126955596 6 0.46288024516459136 1.7028132426849727 7 0.29732642895200256 1.2760822773493414 8 0.21763062910150105 1.0674735596493212 9 0.1521182522649458 0.8394038537507817 >10 0.8597363371089073 9.646397575774476 >50 0.053634588253915455 2.223481664070473 >100 0.025804140022479858 2.9589228280733777 >500 0.002432064466554116 1.055576553377145 >1k 0.0014766105689792838 1.41068398781127 >5k 8.685944523407552E-5 0.3422728675927001 >10k+ 1.7371889046815104E-4 1.1969698104891127 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12355 0.6570490917512818 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10121 0.5382431289044697 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6427 0.3417931616904482 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2520 0.13401567877079967 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2446 0.13008029772753016 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2064 0.10976522261227403 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1990 0.10582984156900452 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.318082490904749E-5 7 0.0 0.0 0.0 0.0 5.318082490904749E-5 8 0.0 0.0 0.0 0.0 5.318082490904749E-5 9 0.0 0.0 0.0 0.0 5.318082490904749E-5 10-11 2.6590412454523746E-5 0.0 0.0 0.0 5.318082490904749E-5 12-13 1.8613288718166622E-4 0.0 0.0 0.0 1.0636164981809498E-4 14-15 2.924945369997612E-4 0.0 0.0 0.0 1.0636164981809498E-4 16-17 3.1908494945428496E-4 0.0 0.0 0.0 1.5954247472714248E-4 18-19 3.4567536190880873E-4 0.0 0.0 2.6590412454523746E-5 1.5954247472714248E-4 20-21 3.7226577436333245E-4 0.0 0.0 1.0636164981809498E-4 2.1272329963618997E-4 22-23 3.7226577436333245E-4 0.0 0.0 3.7226577436333245E-4 2.659041245452375E-4 24-25 3.7226577436333245E-4 0.0 0.0 6.913507238176175E-4 3.1908494945428496E-4 26-27 3.7226577436333245E-4 0.0 0.0 0.0020474617589983286 3.1908494945428496E-4 28-29 4.2544659927237993E-4 0.0 0.0 0.0054244441407228445 3.1908494945428496E-4 30-31 4.2544659927237993E-4 0.0 0.0 0.014066328188443063 3.1908494945428496E-4 32-33 4.2544659927237993E-4 0.0 0.0 0.03137668669633802 3.1908494945428496E-4 34-35 5.052178366359511E-4 0.0 0.0 0.05879140193695201 3.1908494945428496E-4 36-37 6.381698989085699E-4 0.0 0.0 0.1043673688840057 3.1908494945428496E-4 38-39 7.445315487266649E-4 0.0 0.0 0.17073703837049697 3.1908494945428496E-4 40-41 7.445315487266649E-4 0.0 0.0 0.24101549848780324 3.1908494945428496E-4 42-43 7.445315487266649E-4 0.0 0.0 0.3100973900446559 3.1908494945428496E-4 44-45 7.977123736357124E-4 0.0 0.0 0.39901572929258333 3.1908494945428496E-4 46-47 9.838452608173787E-4 0.0 0.0 0.501149503530409 3.1908494945428496E-4 48-49 0.0010104356732719025 0.0 0.0 0.6074313821111404 3.1908494945428496E-4 50-51 0.0010104356732719025 0.0 0.0 0.7209724432919569 3.1908494945428496E-4 52-53 0.0010104356732719025 0.0 0.0 0.8254727642382351 5.052178366359512E-4 54-55 0.0010104356732719025 0.0 0.0 0.9364877362358719 5.31808249090475E-4 56-57 0.0010104356732719025 0.0 0.0 1.06808368747331 5.31808249090475E-4 58-59 0.0010104356732719025 0.0 0.0 1.2098903570932849 5.31808249090475E-4 60-61 0.00106361649818095 0.0 0.0 1.3581584969397094 5.31808249090475E-4 62-63 0.00106361649818095 0.0 0.0 1.5056023340000437 5.31808249090475E-4 64-65 0.00106361649818095 0.0 0.0 1.651078480538743 5.31808249090475E-4 66-67 0.0012231589729080924 0.0 0.0 1.8192362489011513 5.31808249090475E-4 68-69 0.0012231589729080924 0.0 0.0 2.002337829063002 5.31808249090475E-4 70-71 0.0013029302102716637 0.0 0.0 2.20094161968584 5.849890739995224E-4 72-73 0.0013561110351807111 0.0 0.0 2.3959025238024076 6.647603113630936E-4 74-75 0.001382701447635235 0.0 0.0 2.5896402689460674 6.913507238176175E-4 76-77 0.001382701447635235 0.0 0.0 2.7978697888774433 6.913507238176175E-4 78-79 0.001382701447635235 0.0 0.0 3.0338065185864327 6.913507238176175E-4 80-81 0.0014358822725442824 0.0 0.0 3.287372691752771 7.445315487266649E-4 82-83 0.0015156535099078534 0.0 0.0 3.5420556622421993 8.508931985447599E-4 84-85 0.001568834334816901 0.0 0.0 3.800913327486988 8.508931985447599E-4 86-87 0.0015954247472714249 0.0 0.0 4.065275208109863 8.508931985447599E-4 88-89 0.0015954247472714249 0.0 0.0 4.351494407770357 8.508931985447599E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4705 0.0 53.8736 1 GTATCAA 8365 0.0 41.37263 1 ATCAACG 10630 0.0 32.455322 3 TATCAAC 10670 0.0 32.37819 2 TCAACGC 10665 0.0 32.21514 4 CAACGCA 10915 0.0 31.520813 5 AACGCAG 11250 0.0 30.582193 6 TGGTATC 2075 0.0 29.54297 2 GTGGTAT 2410 0.0 27.135925 1 CGCAGAG 12595 0.0 26.91424 8 ACGCAGA 12700 0.0 26.841465 7 GTACATG 8540 0.0 24.426693 1 GCAGAGT 13720 0.0 24.290224 9 TACATGG 8205 0.0 24.093359 2 TAAGGTG 1075 0.0 23.87085 5 ACATGGG 8405 0.0 22.898125 3 GAGTACT 8660 0.0 22.835083 12-13 GTAAGGT 1065 0.0 22.756378 4 CAGAGTA 13450 0.0 21.507147 10-11 GTACTTT 9340 0.0 21.233927 14-15 >>END_MODULE