FastQCFastQC Report
Thu 26 May 2016
SRR938161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938161_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880377
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120920.6430625348002023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104750.5570691409222726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67160.35716242008916294No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25630.13630245424188875No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25010.1330052430975278No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22270.11843369707244877No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA19310.1026921728993707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46700.056.3571931
GTATCAA83650.040.8337671
ATCAACG106450.031.8595543
TCAACGC107550.031.6653544
TATCAAC108250.031.3795092
CAACGCA109400.031.0864665
AACGCAG112950.030.2355796
TGGTATC19250.030.1090562
GTGGTAT22850.027.235891
GCGTATC700.00838698127.1467861
ACGCAGA131000.025.9607567
CGCAGAG132000.025.6201528
GTACATG82950.024.7413751
TACATGG81350.023.8270682
GCAGAGT143950.023.3936849
ACATGGG82500.022.5117873
GAGTACT93900.021.59863712-13
CAGAGTA140750.020.87158210-11
GTACTTT97300.020.64864714-15
GTGTAGC14850.020.4743441
Produced by FastQC (version 0.11.5)