##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938161_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1880377 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51380653985876 34.0 31.0 34.0 31.0 34.0 2 32.72236152643858 34.0 31.0 34.0 31.0 34.0 3 32.77592365786223 34.0 31.0 34.0 31.0 34.0 4 36.234057851164955 37.0 35.0 37.0 35.0 37.0 5 36.20000350993445 37.0 35.0 37.0 35.0 37.0 6 36.24605863611393 37.0 37.0 37.0 35.0 37.0 7 36.21126082695119 37.0 37.0 37.0 35.0 37.0 8 36.2071930256539 37.0 37.0 37.0 35.0 37.0 9 37.91366837607565 39.0 38.0 39.0 35.0 39.0 10-11 37.90409635940027 39.0 38.0 39.0 35.0 39.0 12-13 37.85299463884104 39.0 38.0 39.0 35.0 39.0 14-15 39.167278955230785 40.5 38.5 41.0 36.0 41.0 16-17 39.120362565591904 40.0 38.5 41.0 36.0 41.0 18-19 39.15547600295047 40.0 39.0 41.0 36.0 41.0 20-21 39.092446089268265 40.0 39.0 41.0 36.0 41.0 22-23 38.94922454380159 40.0 38.0 41.0 35.0 41.0 24-25 38.904866417744955 40.0 38.0 41.0 35.0 41.0 26-27 38.86492097063514 40.0 38.0 41.0 35.0 41.0 28-29 38.80284432323944 40.0 38.0 41.0 35.0 41.0 30-31 38.58841391912367 40.0 38.0 41.0 34.5 41.0 32-33 38.39503089008215 40.0 38.0 41.0 34.0 41.0 34-35 38.19743008981709 40.0 38.0 41.0 34.0 41.0 36-37 38.062116001206135 40.0 38.0 41.0 34.0 41.0 38-39 38.037933350599374 40.0 38.0 41.0 34.0 41.0 40-41 37.96428881016945 40.0 38.0 41.0 33.0 41.0 42-43 37.801188006447646 40.0 37.0 41.0 33.0 41.0 44-45 37.57914609676676 40.0 37.0 41.0 33.0 41.0 46-47 37.238116345817886 40.0 37.0 41.0 32.5 41.0 48-49 36.8867256406561 40.0 36.0 41.0 31.0 41.0 50-51 36.58853915996633 40.0 36.0 41.0 31.0 41.0 52-53 36.17785130322271 39.0 35.0 41.0 30.0 41.0 54-55 35.959948723048626 39.0 35.0 41.0 29.0 41.0 56-57 35.72745518584837 39.0 35.0 40.5 29.0 41.0 58-59 35.398467169083645 38.0 35.0 40.0 28.0 41.0 60-61 35.0493669620507 38.0 34.5 40.0 27.5 41.0 62-63 34.69244731242724 37.5 34.0 40.0 26.5 41.0 64-65 34.31732227101267 37.0 34.0 40.0 26.0 41.0 66-67 33.90995077051038 36.0 34.0 39.0 26.0 41.0 68-69 33.45905555109427 36.0 33.0 39.0 25.0 40.5 70-71 32.95920365969165 35.0 33.0 38.5 23.5 40.0 72-73 32.554059106232415 35.0 32.0 37.5 23.0 40.0 74-75 32.11448209587758 35.0 32.0 37.0 22.0 39.0 76-77 30.80980914997365 34.0 30.5 35.5 20.5 38.5 78-79 31.53678810153496 35.0 31.5 36.0 21.5 39.0 80-81 31.372259392664343 35.0 32.0 36.0 20.5 37.5 82-83 31.00915454719984 35.0 31.0 35.0 19.5 37.0 84-85 30.64049815542309 34.0 31.0 35.0 18.0 37.0 86-87 30.208487446932185 34.0 31.0 35.0 13.0 36.0 88-89 29.74963079212307 34.0 30.0 35.0 7.0 36.0 90-91 29.404123215716847 34.0 30.0 35.0 3.5 35.0 92-93 29.002371864790945 34.0 29.0 35.0 2.0 35.0 94-95 28.593464236161154 34.0 29.0 35.0 2.0 35.0 96-97 28.025109060576682 34.0 29.0 35.0 2.0 35.0 98-99 27.349508103960005 33.5 27.0 35.0 2.0 35.0 100-101 24.92747491593441 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19771026825515747 1101 2 0.16452020188815197 1101 3 0.1622817212782337 1101 4 0.09212586031210179 1101 5 0.12594005190872792 1101 6 0.06342086730962393 1101 7 0.13821668103889095 1101 8 0.10054626266848743 1101 9 0.09169215487835913 1101 10-11 0.12583408896231418 1101 12-13 0.2210032491548688 1101 14-15 0.23644951368609668 1101 16-17 0.2524183590477449 1101 18-19 0.2324782717588718 1101 20-21 0.32039232004707685 1101 22-23 0.36901742790412584 1101 24-25 0.2720456558128461 1101 26-27 0.26280608117417614 1101 28-29 0.42175287650236015 1101 30-31 0.40773499405638347 1101 32-33 0.41547897735731 1101 34-35 0.4476344702152133 1101 36-37 0.5193243128379024 1101 38-39 0.5865400503558291 1101 40-41 0.5703266067214514 1101 42-43 0.7046130989019517 1101 44-45 0.7813721345015168 1101 46-47 0.722709805886133 1101 48-49 0.7361494044005852 1101 50-51 0.7525050488869027 1101 52-53 0.7150790566872942 1101 54-55 0.7913714783406007 1101 56-57 0.8193291545909531 1101 58-59 0.6952094291364759 1101 60-61 0.809551030996225 1101 62-63 0.6982291334211084 1101 64-65 0.6669206948663344 1101 66-67 0.60917856285932 1101 68-69 0.6981593251179135 1101 70-71 0.7718574678068606 1101 72-73 0.5219919297272426 1101 74-75 0.6450451836951672 1101 76-77 0.5952383186542853 1101 78-79 0.6306414060879177 1101 80-81 0.771922572260749 1101 82-83 0.6697012478344959 1101 84-85 0.6780044723590066 1101 86-87 0.7049246789941321 1101 88-89 0.7743266955203616 1101 90-91 0.8548505360870244 1101 92-93 1.1480136997628883 1101 94-95 0.9331616142346242 1101 96-97 1.040281456693556 1101 98-99 0.8339534714559669 1101 100-101 1.2677116608170635 1102 1 0.1575324281837993 1102 2 0.1589941751059598 1102 3 0.16769246449319297 1102 4 0.0869608531220436 1102 5 0.06788567447032534 1102 6 0.040402076088163597 1102 7 0.10945191412405819 1102 8 0.11898101520320381 1102 9 0.08935159508562407 1102 10-11 0.218955907287544 1102 12-13 0.14616828668270188 1102 14-15 0.2671096334596541 1102 16-17 0.2528984544297188 1102 18-19 0.21439164525943255 1102 20-21 0.13957914616911893 1102 22-23 0.1497556674633742 1102 24-25 0.1815237853066236 1102 26-27 0.09487053682337887 1102 28-29 0.10248489681653439 1102 30-31 0.13250002794368498 1102 32-33 0.11912512354901139 1102 34-35 0.12065159285872795 1102 36-37 0.05289989335133072 1102 38-39 -0.0666361636104682 1102 40-41 0.054987066278158636 1102 42-43 -0.07868034024956216 1102 44-45 -0.21412573882004438 1102 46-47 -0.20477683032139993 1102 48-49 -0.12933742596874964 1102 50-51 -0.18570978507442248 1102 52-53 -0.2865371885876087 1102 54-55 -0.3556006594414711 1102 56-57 -0.26533069821012845 1102 58-59 -0.13023987339793308 1102 60-61 -0.19073717792186784 1102 62-63 -0.20621595822933614 1102 64-65 -0.20105231664381762 1102 66-67 -0.15492319612521044 1102 68-69 0.03266673057022018 1102 70-71 0.1252941316217644 1102 72-73 0.12094846175484264 1102 74-75 0.13621082191107092 1102 76-77 0.25445630167726563 1102 78-79 0.1750490912545608 1102 80-81 0.19398936391907284 1102 82-83 0.3017312701024295 1102 84-85 0.184974577509756 1102 86-87 0.09299040276257031 1102 88-89 -0.06460850904729298 1102 90-91 0.13995665764444354 1102 92-93 0.22915967909679225 1102 94-95 -0.014821226716527747 1102 96-97 -0.10348625298601632 1102 98-99 0.017017708673140675 1102 100-101 0.040087291084461185 1103 1 0.1628026903213069 1103 2 0.12989166721776257 1103 3 0.15430402124093234 1103 4 0.07109848176200728 1103 5 0.03961067511514216 1103 6 0.10983971418536953 1103 7 0.11137345341889215 1103 8 0.09888008065590981 1103 9 0.12349868096596595 1103 10-11 0.0036414110575435643 1103 12-13 0.07330454832155908 1103 14-15 0.1368189522804215 1103 16-17 0.2144794900835194 1103 18-19 0.17732463641261376 1103 20-21 0.22642670405172538 1103 22-23 0.12805989828881792 1103 24-25 0.1817204529190164 1103 26-27 0.22238960176022715 1103 28-29 0.24799851094392977 1103 30-31 0.21507140861480423 1103 32-33 0.22592745862763053 1103 34-35 0.20721006009798515 1103 36-37 0.3022400358977393 1103 38-39 0.23680154326666525 1103 40-41 0.3604398101674917 1103 42-43 0.3878281325780719 1103 44-45 0.42998433486045684 1103 46-47 0.36022342760333714 1103 48-49 0.38793278458381053 1103 50-51 0.4939218984003375 1103 52-53 0.37714077518257483 1103 54-55 0.34930012550948675 1103 56-57 0.4036637392130231 1103 58-59 0.4757360278340812 1103 60-61 0.47541471395607715 1103 62-63 0.4427914614056192 1103 64-65 0.3540661997730936 1103 66-67 0.3026675289012246 1103 68-69 0.26788012030309716 1103 70-71 0.32699127639492787 1103 72-73 0.14715898852313103 1103 74-75 0.042730299527498516 1103 76-77 0.22577292148760364 1103 78-79 0.2610240037750877 1103 80-81 0.5325897353306956 1103 82-83 0.4464001807143134 1103 84-85 0.39689628875441585 1103 86-87 0.5117580813539568 1103 88-89 0.29591546505706035 1103 90-91 0.23273863386378224 1103 92-93 0.377695311399286 1103 94-95 0.43817521392209713 1103 96-97 0.3542495547053015 1103 98-99 0.35756971059898035 1103 100-101 0.30531237340543527 1104 1 0.06787229914832693 1104 2 0.09620812777633603 1104 3 0.022981423360540987 1104 4 0.01949972786291454 1104 5 0.03008726781886395 1104 6 -0.061863176422818356 1104 7 0.05190109550074595 1104 8 0.01998180869244237 1104 9 -6.488480497210958E-4 1104 10-11 0.028340025316644812 1104 12-13 0.08440837464506501 1104 14-15 0.10902352118300485 1104 16-17 0.10125528778451809 1104 18-19 0.11944660789065864 1104 20-21 0.10041082813087598 1104 22-23 0.1211467801069972 1104 24-25 -0.012760080273807262 1104 26-27 0.14905162269884897 1104 28-29 0.07749258244523816 1104 30-31 0.10360292951086336 1104 32-33 0.061125284185571616 1104 34-35 0.2517443577048013 1104 36-37 0.3161631420596649 1104 38-39 0.2076666944740495 1104 40-41 0.3401631848833375 1104 42-43 0.42389503776566784 1104 44-45 0.4329576343262289 1104 46-47 0.3774114481947066 1104 48-49 0.3326685646672445 1104 50-51 0.4360059934520706 1104 52-53 0.37550823417044654 1104 54-55 0.42908212488871555 1104 56-57 0.41451751408493465 1104 58-59 0.3520902776696673 1104 60-61 0.4076231174278391 1104 62-63 0.3483873179256918 1104 64-65 0.4650853572002518 1104 66-67 0.49709057707534043 1104 68-69 0.3845326419680788 1104 70-71 0.4411464678451793 1104 72-73 0.35688428052356613 1104 74-75 0.46931774077714294 1104 76-77 0.4019815984740909 1104 78-79 0.4432972664370425 1104 80-81 0.4048665711139279 1104 82-83 0.3965762558182391 1104 84-85 0.5901872340092353 1104 86-87 0.6843775479600218 1104 88-89 0.5016310513070472 1104 90-91 0.6045632988585226 1104 92-93 0.644183456077851 1104 94-95 0.41684089781281486 1104 96-97 0.4951309223861813 1104 98-99 0.587646039977745 1104 100-101 0.6853295753363966 1105 1 0.10713065505639463 1105 2 0.05907432088250886 1105 3 0.1238263575416525 1105 4 0.06707582445753246 1105 5 0.08979784305318361 1105 6 0.02292794724925784 1105 7 0.09428948186943131 1105 8 0.08149785364811635 1105 9 -0.01104991698062463 1105 10-11 0.0843103500263922 1105 12-13 0.0810159052827828 1105 14-15 0.08885511449094707 1105 16-17 0.10509019341032655 1105 18-19 0.08546985396215945 1105 20-21 0.17598804104500942 1105 22-23 0.08386121971215488 1105 24-25 0.12005171362967815 1105 26-27 0.07701786930043397 1105 28-29 -0.052736195881060155 1105 30-31 0.040427672232191014 1105 32-33 0.11500296781036212 1105 34-35 0.20922833961896004 1105 36-37 0.16553603674700668 1105 38-39 0.14845473788592756 1105 40-41 0.15821913398698229 1105 42-43 0.19762614033270864 1105 44-45 0.17654902596385114 1105 46-47 0.10864210496675497 1105 48-49 0.22322436720815375 1105 50-51 0.2455234417459593 1105 52-53 0.17805051752018386 1105 54-55 0.1986706927509303 1105 56-57 0.08251306712720918 1105 58-59 0.16704352415644053 1105 60-61 0.20569920669431951 1105 62-63 0.3281109738843213 1105 64-65 0.25294022222819024 1105 66-67 0.18029369348768398 1105 68-69 0.19925703684717178 1105 70-71 0.2977824621878611 1105 72-73 0.2125496020699984 1105 74-75 0.2663413263856569 1105 76-77 0.35336780716476923 1105 78-79 0.31159125785335107 1105 80-81 0.377987881091677 1105 82-83 0.48213301712372214 1105 84-85 0.6922118455569155 1105 86-87 0.6072156254477399 1105 88-89 0.415763607532174 1105 90-91 0.3116069818824059 1105 92-93 0.3658924203434104 1105 94-95 0.27857758075608885 1105 96-97 0.43067751590175973 1105 98-99 0.5446425481205281 1105 100-101 0.8235625111590181 1106 1 -0.1299938084629204 1106 2 0.008108655646459795 1106 3 -0.018877505119291982 1106 4 0.011818313727914642 1106 5 -0.048614174114199216 1106 6 -0.04842398408121085 1106 7 -0.08060322199322911 1106 8 -0.16099141673814898 1106 9 -0.20686536762439545 1106 10-11 -0.1329643280852011 1106 12-13 -0.1848102749747227 1106 14-15 -0.2451041129768612 1106 16-17 -0.1733432829845185 1106 18-19 -0.04409023187474048 1106 20-21 0.03891722559436772 1106 22-23 0.04684405233017941 1106 24-25 0.009268451827097124 1106 26-27 -0.034033632845492434 1106 28-29 0.007617881372155466 1106 30-31 0.02272380957123943 1106 32-33 0.018972924891905052 1106 34-35 0.01636353275200264 1106 36-37 -0.021427482566295453 1106 38-39 -0.15894869559046754 1106 40-41 -0.019892453995851156 1106 42-43 -0.08992750773595759 1106 44-45 -0.042603334551174044 1106 46-47 -0.07671197228646065 1106 48-49 -0.05333142678326652 1106 50-51 -0.005013038940738568 1106 52-53 -0.2589130017931254 1106 54-55 -0.5422091355752556 1106 56-57 -0.3413066753620626 1106 58-59 -0.2717976775602935 1106 60-61 -0.1655454284988096 1106 62-63 -0.2775757213946406 1106 64-65 -0.43670570051859414 1106 66-67 -0.5401998687440752 1106 68-69 -0.5716657099339386 1106 70-71 -0.5930437180696586 1106 72-73 -0.37516499020468075 1106 74-75 -0.23312219292764524 1106 76-77 -0.28423948897257034 1106 78-79 -0.1527327764814146 1106 80-81 -0.2778597583933049 1106 82-83 -0.4687790000436607 1106 84-85 -0.24475167375639018 1106 86-87 -0.1457350183290842 1106 88-89 -0.09818489032189959 1106 90-91 -0.1629101854995696 1106 92-93 -0.08394663545057668 1106 94-95 -0.25387950078897603 1106 96-97 -0.14308857851884227 1106 98-99 0.006820659708512977 1106 100-101 0.06068697467832962 1107 1 0.03871861583430558 1107 2 0.060447777604565545 1107 3 0.005655045419068472 1107 4 0.026169861306527764 1107 5 -0.023558375711843382 1107 6 0.02652120997813512 1107 7 -0.0329926286775688 1107 8 -0.031790546900602124 1107 9 -0.19826127603562327 1107 10-11 -0.06805650572261612 1107 12-13 -0.06197218738225274 1107 14-15 0.0454627590926151 1107 16-17 -0.0224154967034238 1107 18-19 0.03499407884957151 1107 20-21 0.008446721556147452 1107 22-23 -0.0462637422434824 1107 24-25 -0.054441248714127255 1107 26-27 -0.11913667242486525 1107 28-29 -0.13742984500836286 1107 30-31 -0.0627982693934186 1107 32-33 -0.024980447051859755 1107 34-35 -0.013214307122019875 1107 36-37 -0.03400555622018686 1107 38-39 -0.14447253893148826 1107 40-41 -0.10040342841681138 1107 42-43 -0.10553810325708923 1107 44-45 -0.12092344976494473 1107 46-47 -0.21880491963305815 1107 48-49 -0.19581667218596266 1107 50-51 -0.1933378848886349 1107 52-53 -0.024228891325513757 1107 54-55 -0.03652922211791321 1107 56-57 0.06423109552484618 1107 58-59 -0.030538508316901414 1107 60-61 -0.008435157181843067 1107 62-63 -0.004782735672861804 1107 64-65 -0.1360609312328549 1107 66-67 -0.3046920150638073 1107 68-69 -0.17257397780156225 1107 70-71 -0.249249195134972 1107 72-73 -0.23783448060868295 1107 74-75 -0.2791467773067424 1107 76-77 -0.23218983501088175 1107 78-79 -0.23964209635843403 1107 80-81 -0.20076230085963687 1107 82-83 -0.11288333643965842 1107 84-85 -0.1584687074868576 1107 86-87 -0.19154015839851368 1107 88-89 -0.13926067469746428 1107 90-91 -0.16941273943434965 1107 92-93 0.024994231475744755 1107 94-95 -0.18563921257809923 1107 96-97 -0.11011817372679644 1107 98-99 -0.026150572134955752 1107 100-101 0.39783310694480534 1108 1 0.07740002454566763 1108 2 0.07175484786091602 1108 3 0.055113191154639196 1108 4 0.02174797939162687 1108 5 -0.07333837885726524 1108 6 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100-101 -0.24495155546711445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 9.0 4 13.0 5 42.0 6 68.0 7 170.0 8 443.0 9 721.0 10 1359.0 11 2157.0 12 3187.0 13 4941.0 14 6585.0 15 8976.0 16 11916.0 17 16710.0 18 16962.0 19 11549.0 20 9665.0 21 9608.0 22 9367.0 23 10409.0 24 11566.0 25 13884.0 26 16641.0 27 19944.0 28 24420.0 29 30136.0 30 36965.0 31 45410.0 32 57397.0 33 74995.0 34 105693.0 35 166775.0 36 265925.0 37 436502.0 38 400415.0 39 48847.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78393269009353 18.837658618457894 14.745447322531598 22.63296136891698 2 15.960729268003385 19.94689579203405 40.176599149928435 23.915775790034132 3 19.562610191756924 24.172807267138058 29.78952211015054 26.47506043095448 4 12.868536469016586 16.88672005666949 36.437267633033166 33.80747584128076 5 13.495378852219527 37.43961982091889 34.11124471316124 14.953756613700337 6 32.28423464243321 35.99939586550775 17.00170604177037 14.714663450288665 7 28.62101589202591 33.188823305113814 19.91845252308447 18.27170827977581 8 26.716628411824892 35.05673345486957 19.029798359048105 19.196839774257434 9 26.420931210842124 16.75522198036662 20.027505239663064 36.79634156912819 10-11 25.31245064154688 26.77197179076323 26.43318334568015 21.482394222009734 12-13 26.153452206658557 24.076182595298707 27.373659643784197 22.396705554258535 14-15 22.768279977898047 25.435909926573235 26.03945910846602 25.7563509870627 16-17 21.87973975362322 28.703921351732532 26.986016596715967 22.43032229792828 18-19 21.80419139353438 26.716530780795555 29.861724537153982 21.617553288516078 20-21 22.41236730719425 25.811313369606204 30.330380556664966 21.445938766534585 22-23 22.06110795866999 25.891829138518503 30.202427491933797 21.844635410877714 24-25 21.868822379705097 26.118147611386632 30.29200515881985 21.72102485008842 26-27 21.494124571374165 26.483910424015438 30.179062075047717 21.842902929562676 28-29 21.56664985264911 26.73593651906228 30.09885525524024 21.598558373048363 30-31 21.688177423995295 26.407071560649808 30.00435550956005 21.900395505794847 32-33 21.39813849921119 26.419877635426538 29.95076220594465 22.23122165941763 34-35 22.0320091317886 26.548037101614554 29.82581091963622 21.594142846960626 36-37 22.13257912583976 26.197896501401907 29.770179801478065 21.899344571280267 38-39 21.720279173500483 26.49629703898754 29.783790267224347 21.99963352028763 40-41 22.033670674801726 26.398454757291262 29.835465586010905 21.73240898189611 42-43 21.874739059652093 26.3707046891878 29.62101755167882 22.133538699481285 44-45 21.788509013862488 26.649368247846382 29.66839260851576 21.893730129775367 46-47 22.145325683446906 26.877463103798625 28.997331119592275 21.979880093162194 48-49 21.982267385742325 27.00559515458868 28.87442252271752 22.137714936951472 50-51 22.16645917281481 27.24121279934821 28.68164203242222 21.91068599541475 52-53 22.371657385726373 27.032185567043204 28.634949268152077 21.961207779078347 54-55 21.883297870586592 27.177422399869812 28.835600520533916 22.103679209009684 56-57 21.975912277165698 27.41670952154807 28.507660963732274 22.09971723755396 58-59 21.979103126660238 27.084621860403523 28.568154152066317 22.368120860869922 60-61 21.830170226502453 27.213851264932508 28.82203941018211 22.13393909838293 62-63 21.8636475557827 27.186277007217168 28.88003309974542 22.070042337254712 64-65 21.624812471116165 27.174656996974544 28.843471282620452 22.357059249288838 66-67 21.595376884529006 27.363129840452206 28.634896087327167 22.406597187691617 68-69 22.024716924288633 27.00431802970849 28.74897327469598 22.221991771306893 70-71 21.79945830011748 27.166892596537824 28.471817087743574 22.561832015601126 72-73 21.797410838358477 27.239457832126217 28.39919867133027 22.563932658185035 74-75 21.78789147069976 27.542535353282876 28.200328976582888 22.469244199434474 76-77 21.788742363898304 27.247727450399573 28.43041581555188 22.533114370150244 78-79 21.868114745075058 27.36738430644493 28.355803118204488 22.408697830275525 80-81 21.879734755317685 27.485605280217744 28.180253215179725 22.45440674928485 82-83 21.899491431771395 27.490790410646376 28.171531559894635 22.438186597687594 84-85 21.825186417439983 27.590100211506215 28.09424231900583 22.490471052047972 86-87 21.96209631979638 27.45784543107572 28.053844679096656 22.526213570031242 88-89 21.843270134076946 27.552794641283086 27.824735006385694 22.77920021825428 90-91 21.72662019716301 27.570620297143172 28.00391198980202 22.698847515891792 92-93 21.8679219495281 27.286820221307156 28.145745551547485 22.69951227761726 94-95 21.637868363631334 27.17957622327863 28.183975872923355 22.99857954016668 96-97 21.864338906506514 26.885007634107417 28.19123505552344 23.05941840386263 98-99 21.556395339870672 27.09512507332306 27.952426561269363 23.3960530255369 100-101 21.69790951495365 26.886842372566775 28.20413140556389 23.21111670691569 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2840.0 1 1794.5 2 1234.5 3 1627.5 4 1161.5 5 953.5 6 1858.5 7 3941.5 8 6049.5 9 6661.0 10 5074.0 11 3178.5 12 2716.5 13 2762.5 14 2840.0 15 2884.0 16 2943.5 17 3089.0 18 3302.0 19 3569.5 20 3916.0 21 4446.5 22 5243.5 23 6264.5 24 7610.0 25 9458.5 26 11812.0 27 14364.5 28 16809.5 29 19284.5 30 21768.0 31 25404.5 32 31012.5 33 33944.5 34 34609.0 35 36864.0 36 40794.0 37 44561.0 38 47409.5 39 50021.5 40 52512.0 41 56088.5 42 58089.0 43 59249.0 44 61394.5 45 64834.5 46 66731.0 47 67415.0 48 68975.5 49 68467.0 50 66888.0 51 65154.5 52 64132.5 53 64626.5 54 64954.0 55 61177.5 56 55770.0 57 51112.0 58 46943.0 59 41606.5 60 34293.5 61 28448.0 62 23388.0 63 17039.5 64 11622.0 65 8815.0 66 6726.5 67 5282.5 68 3978.5 69 2769.5 70 2011.0 71 1417.5 72 1053.0 73 864.0 74 663.5 75 472.5 76 333.5 77 192.5 78 105.0 79 56.5 80 27.5 81 16.5 82 7.5 83 3.5 84 1.0 85 1.0 86 2.0 87 1.0 88 2.5 89 2.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015954247472714247 3 0.0070198688879942696 4 0.0 5 0.0 6 5.31808249090475E-5 7 0.0 8 2.659041245452375E-4 9 4.2544659927238E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.659041245452375E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0054244441407228445 26-27 0.020554388827346858 28-29 7.977123736357124E-5 30-31 0.0 32-33 6.647603113630936E-4 34-35 0.01866646954307567 36-37 0.004307646817632847 38-39 0.017416720157713055 40-41 0.015103354274169489 42-43 0.008402570335629503 44-45 0.0027122220703614222 46-47 2.659041245452375E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.924945369997612E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0028717645450885647 86-87 2.924945369997612E-4 88-89 7.977123736357124E-5 90-91 2.1272329963619E-4 92-93 2.1272329963619E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1880377.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.565772225988354 #Duplication Level Percentage of deduplicated Percentage of total 1 78.59473463731432 46.81556061568263 2 13.049369728578888 15.545915698904752 3 3.7732882938252312 6.742764931589459 4 1.572372205257088 3.746382585312749 5 0.8007736535325155 2.3849350525445168 6 0.47425784369729873 1.6949720816437013 7 0.3191850368169667 1.3308752240688204 8 0.22880306979721904 1.0903065232118452 9 0.17302291881056955 0.9275619394571456 >10 0.9205604240481868 10.011152244689454 >50 0.061483640522445294 2.4610347912550274 >100 0.027862913440333033 3.183462905338836 >500 0.0024999533761367663 1.066845630990635 >1k 0.0015178288355116073 1.4408797946375502 >5k 8.928404914774162E-5 0.3571752371751296 >10k+ 1.7856809829548323E-4 1.200174743497789 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12092 0.6430625348002023 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10475 0.5570691409222726 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6716 0.35716242008916294 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2563 0.13630245424188875 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2501 0.1330052430975278 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2227 0.11843369707244877 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1931 0.1026921728993707 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 5.318082490904749E-5 9 0.0 0.0 0.0 0.0 5.318082490904749E-5 10-11 0.0 0.0 0.0 0.0 5.318082490904749E-5 12-13 0.0 0.0 0.0 0.0 1.8613288718166622E-4 14-15 5.318082490904749E-5 0.0 0.0 5.318082490904749E-5 2.659041245452375E-4 16-17 5.318082490904749E-5 0.0 0.0 5.318082490904749E-5 3.1908494945428496E-4 18-19 7.977123736357124E-5 0.0 0.0 7.977123736357124E-5 3.1908494945428496E-4 20-21 1.5954247472714248E-4 0.0 0.0 1.5954247472714248E-4 3.1908494945428496E-4 22-23 1.5954247472714248E-4 0.0 0.0 4.2544659927238E-4 3.1908494945428496E-4 24-25 2.659041245452375E-4 0.0 0.0 7.445315487266649E-4 3.1908494945428496E-4 26-27 2.659041245452375E-4 0.0 0.0 0.0021006425839073762 3.1908494945428496E-4 28-29 2.924945369997612E-4 0.0 0.0 0.0054776249656318925 3.1908494945428496E-4 30-31 3.1908494945428496E-4 0.0 0.0 0.014039737775988539 3.7226577436333245E-4 32-33 3.1908494945428496E-4 0.0 0.0 0.03132350587142897 3.7226577436333245E-4 34-35 3.1908494945428496E-4 0.0 0.0 0.05881799234940653 4.2544659927237993E-4 36-37 3.7226577436333245E-4 0.0 0.0 0.10450032094627831 4.2544659927237993E-4 38-39 4.2544659927237993E-4 0.0 0.0 0.17102953290749673 4.2544659927237993E-4 40-41 4.2544659927237993E-4 0.0 0.0 0.24154730673689373 4.2544659927237993E-4 42-43 5.31808249090475E-4 0.0 0.0 0.3112141873677459 4.2544659927237993E-4 44-45 6.913507238176175E-4 0.0 0.0 0.40071751568967284 4.2544659927237993E-4 46-47 8.508931985447599E-4 0.0 0.0 0.5032501461143164 4.2544659927237993E-4 48-49 8.508931985447599E-4 0.0 0.0 0.6101436041815018 5.31808249090475E-4 50-51 8.508931985447599E-4 0.0 0.0 0.7243760160861359 5.31808249090475E-4 52-53 8.508931985447599E-4 0.0 0.0 0.8292751932192322 5.849890739995224E-4 54-55 8.508931985447599E-4 0.0 0.0 0.9407953830535047 7.711219611811887E-4 56-57 9.040740234538074E-4 0.0 0.0 1.0728167808902151 7.977123736357124E-4 58-59 9.040740234538074E-4 0.0 0.0 1.2154743437087352 7.977123736357124E-4 60-61 9.040740234538074E-4 0.0 0.0 1.3643540630416133 7.977123736357124E-4 62-63 9.040740234538074E-4 0.0 0.0 1.5124094795884018 8.508931985447599E-4 64-65 9.040740234538074E-4 0.0 0.0 1.659002423450191 8.508931985447599E-4 66-67 9.57254848362855E-4 0.0 0.0 1.8283301699605983 8.508931985447599E-4 68-69 9.57254848362855E-4 0.0 0.0 2.012415595383266 8.508931985447599E-4 70-71 9.57254848362855E-4 0.0 0.0 2.2128807148779206 8.774836109992836E-4 72-73 9.838452608173787E-4 0.0 0.0 2.408400017656034 9.838452608173787E-4 74-75 0.0010104356732719025 0.0 0.0 2.6044511286832375 0.0010104356732719025 76-77 0.0010104356732719025 0.0 0.0 2.8152333282102475 0.0010104356732719025 78-79 0.0010104356732719025 0.0 0.0 3.053270700503144 0.0010104356732719025 80-81 0.0011433877355445212 0.0 0.0 3.310426579350843 0.0010104356732719025 82-83 0.0011699781479990448 0.0 0.0 3.568326989747269 0.0010104356732719025 84-85 0.0011699781479990448 0.0 0.0 3.8312795785100544 0.0010370260857264263 86-87 0.0011699781479990448 0.0 0.0 4.100028877187926 0.0010902069106354735 88-89 0.0011699781479990448 0.0 0.0 4.3917788826389605 0.0011167973230899973 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4670 0.0 56.357193 1 GTATCAA 8365 0.0 40.833767 1 ATCAACG 10645 0.0 31.859554 3 TCAACGC 10755 0.0 31.665354 4 TATCAAC 10825 0.0 31.379509 2 CAACGCA 10940 0.0 31.086466 5 AACGCAG 11295 0.0 30.235579 6 TGGTATC 1925 0.0 30.109056 2 GTGGTAT 2285 0.0 27.23589 1 GCGTATC 70 0.008386981 27.146786 1 ACGCAGA 13100 0.0 25.960756 7 CGCAGAG 13200 0.0 25.620152 8 GTACATG 8295 0.0 24.741375 1 TACATGG 8135 0.0 23.827068 2 GCAGAGT 14395 0.0 23.393684 9 ACATGGG 8250 0.0 22.511787 3 GAGTACT 9390 0.0 21.598637 12-13 CAGAGTA 14075 0.0 20.871582 10-11 GTACTTT 9730 0.0 20.648647 14-15 GTGTAGC 1485 0.0 20.474344 1 >>END_MODULE