FastQCFastQC Report
Thu 26 May 2016
SRR938160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847342
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117490.6359948509804898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95280.5157680602725428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61390.33231529408198374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25340.1371700529734072No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24140.13067423357450866No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19110.10344592392745901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47550.058.379891
GTATCAA84750.040.9574621
ATCAACG108400.032.1580163
TATCAAC108550.031.937472
TCAACGC110150.031.5175864
CAACGCA112950.030.7783765
AACGCAG116300.029.8845126
ACGCAGA131700.026.2059827
CGCAGAG131450.026.1069158
TGGTATC19750.026.0051562
GTGGTAT21900.024.7859731
GTGTAGC13050.024.4460961
GCAGAGT140800.023.8999929
GAGTACT90000.023.79395112-13
GTAAGGT7650.023.6230934
GTACATG84100.023.1564791
TACATGG83450.022.9657942
TAAGGTG9400.022.7666555
GTACTTT95300.022.2209814-15
CAGAGTA137200.021.98026310-11
Produced by FastQC (version 0.11.5)