##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938160_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1847342 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95600056730156 33.0 31.0 34.0 30.0 34.0 2 32.061263696705865 33.0 31.0 34.0 30.0 34.0 3 32.08044639270909 34.0 31.0 34.0 30.0 34.0 4 35.60411607596211 37.0 35.0 37.0 33.0 37.0 5 35.613649773566564 37.0 35.0 37.0 33.0 37.0 6 35.64495908175097 37.0 35.0 37.0 33.0 37.0 7 35.58268799171999 37.0 35.0 37.0 33.0 37.0 8 35.571920088429756 37.0 35.0 37.0 33.0 37.0 9 37.20488518097894 39.0 37.0 39.0 34.0 39.0 10-11 37.1140362748208 39.0 37.0 39.0 34.0 39.0 12-13 36.967194488080715 39.0 37.0 39.0 33.0 39.0 14-15 38.137047444382254 40.0 38.0 41.0 33.0 41.0 16-17 37.95956596017413 40.0 38.0 41.0 33.0 41.0 18-19 38.14126620842269 40.0 38.0 41.0 33.0 41.0 20-21 38.14312861397619 40.0 38.0 41.0 33.0 41.0 22-23 38.02970592342945 40.0 38.0 41.0 33.0 41.0 24-25 37.906757113734216 40.0 38.0 41.0 33.0 41.0 26-27 37.72481706148618 40.0 38.0 41.0 32.5 41.0 28-29 37.53824278341531 40.0 37.0 41.0 32.0 41.0 30-31 37.29558305933607 40.0 37.0 41.0 31.5 41.0 32-33 36.98202498508668 40.0 36.0 41.0 31.0 41.0 34-35 36.73938014726023 39.0 36.0 41.0 30.0 41.0 36-37 36.470435631301626 39.0 35.0 41.0 30.0 41.0 38-39 36.16613139310425 39.0 35.0 40.5 30.0 41.0 40-41 36.040139562679784 39.0 35.0 41.0 29.5 41.0 42-43 35.81499743956452 39.0 35.0 41.0 28.5 41.0 44-45 35.46910507096142 39.0 35.0 40.0 26.5 41.0 46-47 35.061843719246355 38.0 35.0 40.0 25.0 41.0 48-49 34.644660544717766 38.0 34.0 40.0 23.5 41.0 50-51 33.90558705426499 37.5 33.5 39.5 22.5 40.5 52-53 33.98141248344919 38.0 33.5 40.0 23.0 40.5 54-55 34.06191869182858 38.0 34.0 40.0 22.5 41.0 56-57 33.7585631139226 38.0 33.0 40.0 20.5 41.0 58-59 33.35111473674068 37.5 33.0 40.0 18.5 41.0 60-61 32.86013688856747 37.0 32.5 40.0 14.0 41.0 62-63 32.32531713131624 36.0 31.5 39.0 8.5 41.0 64-65 31.800050017809372 35.5 31.0 39.0 7.0 40.5 66-67 31.2482233933944 35.0 30.5 39.0 2.0 40.0 68-69 30.589155662568167 35.0 29.5 38.0 2.0 40.0 70-71 30.018232141097858 35.0 29.0 37.5 2.0 40.0 72-73 29.33929667598095 34.0 28.0 37.0 2.0 39.0 74-75 28.676635403731417 34.0 27.0 36.0 2.0 39.0 76-77 27.94911364544302 34.0 26.0 35.5 2.0 38.5 78-79 27.209015168820933 33.0 25.0 35.0 2.0 37.0 80-81 26.500647957985038 32.5 23.5 35.0 2.0 37.0 82-83 25.779667219172197 32.0 20.5 35.0 2.0 36.0 84-85 25.0668909709193 31.5 18.5 35.0 2.0 36.0 86-87 24.328534185873544 31.0 11.5 34.0 2.0 35.0 88-89 23.458189929098133 30.5 4.5 34.0 2.0 35.0 90-91 22.58465730763443 30.0 2.0 34.0 2.0 35.0 92-93 21.668850434840977 29.0 2.0 34.0 2.0 35.0 94-95 20.608543518200747 29.0 2.0 34.0 2.0 35.0 96-97 19.369652993327712 26.5 2.0 33.5 2.0 35.0 98-99 17.84052898705275 24.0 2.0 33.0 2.0 35.0 100-101 13.922530587189595 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09035871436142884 1101 2 0.03223083465547205 1101 3 0.017944417711973415 1101 4 -0.10401348955852541 1101 5 0.02264400947758105 1101 6 0.011110575494178931 1101 7 -0.05669004216878193 1101 8 0.11747015927817017 1101 9 0.1215824991207981 1101 10-11 0.1721845586196622 1101 12-13 0.1592901250657519 1101 14-15 0.14795039647265895 1101 16-17 0.17489468665993257 1101 18-19 0.21294643988463946 1101 20-21 0.27623805904072896 1101 22-23 0.27926154824709215 1101 24-25 0.3112171853827945 1101 26-27 0.22329132924881634 1101 28-29 0.22278184696735082 1101 30-31 0.2428820819984665 1101 32-33 0.12004583918065492 1101 34-35 0.21873637181723637 1101 36-37 0.18539852448652283 1101 38-39 0.2204740828374625 1101 40-41 0.2160646722884536 1101 42-43 0.08092834915606062 1101 44-45 0.2712069450505794 1101 46-47 0.22483801980939688 1101 48-49 0.1399865579758881 1101 50-51 0.18515628794887817 1101 52-53 0.2514698154963 1101 54-55 0.19670207790033345 1101 56-57 0.011303214427172747 1101 58-59 -0.06128832803049278 1101 60-61 0.010428460915271387 1101 62-63 -0.21471848940469584 1101 64-65 -0.5498532357350818 1101 66-67 -0.5351250885463585 1101 68-69 -0.23252853999747458 1101 70-71 -0.10806598788025923 1101 72-73 -0.16501707000913868 1101 74-75 -0.20267928283990244 1101 76-77 -0.35279190985776765 1101 78-79 -0.5934502008127183 1101 80-81 -0.5879830950574494 1101 82-83 -0.5483133989006674 1101 84-85 -0.728014306605008 1101 86-87 -0.6656024645954624 1101 88-89 -0.7091905078507175 1101 90-91 -0.5528322987524739 1101 92-93 -0.8920917923575828 1101 94-95 -1.0751999030250623 1101 96-97 -0.9939462199421136 1101 98-99 -1.269692441564029 1101 100-101 -1.4109428595231623 1102 1 0.24307510379340158 1102 2 0.28216562407915546 1102 3 0.281078465928708 1102 4 0.33844405975224134 1102 5 0.19986721152028508 1102 6 0.16995476489280748 1102 7 0.18021020997878168 1102 8 0.24697317890674952 1102 9 0.29386963943561284 1102 10-11 0.4046734249607127 1102 12-13 0.4468476280311364 1102 14-15 0.40800838004199136 1102 16-17 0.38340759835404725 1102 18-19 0.34191122298269505 1102 20-21 0.38898116287271733 1102 22-23 0.41024127832715607 1102 24-25 0.4244111794687768 1102 26-27 0.26072140237241115 1102 28-29 0.3429422051588773 1102 30-31 0.3545923859076723 1102 32-33 0.4616930224427165 1102 34-35 0.3342978887229222 1102 36-37 0.49842983114692885 1102 38-39 0.5215770389704844 1102 40-41 0.46763411337598626 1102 42-43 0.43481797058374383 1102 44-45 0.5626870536311728 1102 46-47 0.7417865876096528 1102 48-49 0.553264069460532 1102 50-51 0.6501063011505224 1102 52-53 0.64957579724053 1102 54-55 0.5838118015750098 1102 56-57 0.6098232334758364 1102 58-59 0.6510494731247078 1102 60-61 0.6405656384419913 1102 62-63 0.4542371283169402 1102 64-65 0.3815887116960148 1102 66-67 0.4129335906024494 1102 68-69 0.2796833867975721 1102 70-71 -0.11544176272947837 1102 72-73 0.18972427732512287 1102 74-75 0.3411452157565762 1102 76-77 0.12063627594810455 1102 78-79 0.3141216843751877 1102 80-81 0.2556818270767387 1102 82-83 0.0656724948750913 1102 84-85 0.1097912358552513 1102 86-87 -0.025422260053613144 1102 88-89 0.08019548372096708 1102 90-91 -0.17738529720258 1102 92-93 -0.5761435587864554 1102 94-95 -0.9318134121038923 1102 96-97 -1.023270040109292 1102 98-99 -1.0432041447685627 1102 100-101 -1.0356833134525871 1103 1 0.3313677644064654 1103 2 0.25665965533902124 1103 3 0.25993984972856055 1103 4 0.1724625415123171 1103 5 0.30076362862644856 1103 6 0.22914948927492418 1103 7 0.2853000054166088 1103 8 0.32109369054089854 1103 9 0.2951013318372162 1103 10-11 0.35404486814426406 1103 12-13 0.24123448517956803 1103 14-15 0.296372242599368 1103 16-17 0.2773277621897492 1103 18-19 0.35936019867205005 1103 20-21 0.42796732067483134 1103 22-23 0.2909877701597381 1103 24-25 0.34924119841846846 1103 26-27 0.2980692405553924 1103 28-29 0.29123343348427966 1103 30-31 0.40774038171729643 1103 32-33 0.3516468367897474 1103 34-35 0.19108674477501353 1103 36-37 0.15069682006582497 1103 38-39 0.0847383003134965 1103 40-41 0.3781335693316379 1103 42-43 0.26590513691133566 1103 44-45 0.24331540047875677 1103 46-47 0.4613905938157359 1103 48-49 0.3977652471609332 1103 50-51 0.485005714812452 1103 52-53 0.49340357477251473 1103 54-55 0.47836224697147856 1103 56-57 0.500528623901701 1103 58-59 0.42845528521306875 1103 60-61 0.5045901718049208 1103 62-63 0.2267002356612764 1103 64-65 0.08918156105610464 1103 66-67 0.024900054074244338 1103 68-69 0.12334150198199723 1103 70-71 -0.13981427450460515 1103 72-73 -0.22134509414055614 1103 74-75 -0.3059074211899997 1103 76-77 -0.192716207046292 1103 78-79 -0.3424639143098034 1103 80-81 -0.31693178295557445 1103 82-83 -0.42919507850715277 1103 84-85 -0.5927211166884838 1103 86-87 -0.9100644243527576 1103 88-89 -0.7231906017918561 1103 90-91 -0.8192125213166044 1103 92-93 -1.1515280080418577 1103 94-95 -1.2877438329190056 1103 96-97 -1.3917674728648883 1103 98-99 -1.3205833358034944 1103 100-101 -1.1129949769984684 1104 1 0.36649477427593524 1104 2 0.22759594570553077 1104 3 0.26048454170525304 1104 4 0.23139786197569379 1104 5 0.24132262316020103 1104 6 0.2704146506003866 1104 7 0.27153440921609473 1104 8 0.3332782743523026 1104 9 0.351834691058059 1104 10-11 0.35539500141808844 1104 12-13 0.3618241933125219 1104 14-15 0.3676769907320434 1104 16-17 0.2596493448186479 1104 18-19 0.5970026312312839 1104 20-21 0.6010481160656767 1104 22-23 0.5769370013533717 1104 24-25 0.5400315113352221 1104 26-27 0.46775695634566716 1104 28-29 0.23903647989379806 1104 30-31 0.4998803007866073 1104 32-33 0.5564195684551478 1104 34-35 0.43541658915124515 1104 36-37 0.4865203328370953 1104 38-39 0.5179177728056672 1104 40-41 0.43913239385121017 1104 42-43 0.4478536933897388 1104 44-45 0.3800812303696901 1104 46-47 0.366184937453113 1104 48-49 0.4934859010021597 1104 50-51 0.3853616973752807 1104 52-53 0.43003340737637075 1104 54-55 0.3620531149469244 1104 56-57 0.3943785243636384 1104 58-59 0.5321073570632109 1104 60-61 0.5800402015872734 1104 62-63 0.6034691228555644 1104 64-65 0.5015759391320778 1104 66-67 0.506005392231323 1104 68-69 0.483276771490317 1104 70-71 0.3905595071863637 1104 72-73 0.3903520782052574 1104 74-75 0.1449856100911333 1104 76-77 0.20168681933261112 1104 78-79 0.006009454645212742 1104 80-81 -0.13550033244084503 1104 82-83 -0.28264798637162514 1104 84-85 -0.00847536572516816 1104 86-87 -0.23960219171305752 1104 88-89 -0.1019869730890477 1104 90-91 -0.3050085359284047 1104 92-93 -0.501275780515229 1104 94-95 -0.7655346325443944 1104 96-97 -0.9367794121616022 1104 98-99 -1.3186509092358776 1104 100-101 -1.5962718790716366 1105 1 0.11608758668940311 1105 2 0.15311281204591154 1105 3 0.1687480643032444 1105 4 0.07491071816057371 1105 5 0.22372097639418342 1105 6 0.1180981352320174 1105 7 0.16140826613828807 1105 8 0.13925747625795992 1105 9 0.17270851795412767 1105 10-11 0.22136372754315659 1105 12-13 0.19543410059852562 1105 14-15 0.1688937159496433 1105 16-17 0.2583650608249073 1105 18-19 0.2142940080624527 1105 20-21 0.2587985255886238 1105 22-23 0.3442895981125531 1105 24-25 0.3192509322313981 1105 26-27 0.322438195690566 1105 28-29 0.2516267667767167 1105 30-31 0.12601670612591676 1105 32-33 0.3223322458177833 1105 34-35 0.27467023982733707 1105 36-37 0.3650666681488204 1105 38-39 0.24128960630081764 1105 40-41 0.31128466632826246 1105 42-43 0.4958189231396233 1105 44-45 0.5272954734278201 1105 46-47 0.42812232297851693 1105 48-49 0.45954837535484216 1105 50-51 0.404501015248492 1105 52-53 0.43192104962512445 1105 54-55 0.396785498787537 1105 56-57 0.37317040663455003 1105 58-59 0.38616738929125205 1105 60-61 0.4926631835816053 1105 62-63 0.6293404642559253 1105 64-65 0.5446989561568891 1105 66-67 0.5940494905969018 1105 68-69 0.25048057213020414 1105 70-71 0.2961620480635929 1105 72-73 0.20646092623637813 1105 74-75 0.12307221210155106 1105 76-77 0.058985335708523934 1105 78-79 -0.09695779352736622 1105 80-81 0.08829220039605801 1105 82-83 -0.10773147567398667 1105 84-85 -0.005716131858370943 1105 86-87 0.21346280817017416 1105 88-89 0.16433222461295216 1105 90-91 -0.021590795827851395 1105 92-93 -0.4229297861668151 1105 94-95 -0.5906980246955698 1105 96-97 -0.6359265034643649 1105 98-99 -0.7597781899701168 1105 100-101 -1.0147512894920343 1106 1 0.34451229783947923 1106 2 0.4204860781635844 1106 3 0.4301815114022176 1106 4 0.321655872683543 1106 5 0.3169680983321612 1106 6 0.289629587779217 1106 7 0.38849388261238715 1106 8 0.37395912846908885 1106 9 0.609630165781617 1106 10-11 0.5119744089889124 1106 12-13 0.5552748286986358 1106 14-15 0.6147287453304031 1106 16-17 0.15292779370526688 1106 18-19 0.6017296887254275 1106 20-21 0.7186147262336462 1106 22-23 0.703687788299483 1106 24-25 0.6025352106545441 1106 26-27 0.43298604510242455 1106 28-29 0.544611914619864 1106 30-31 0.37834263947386404 1106 32-33 0.4685398837716761 1106 34-35 0.3953374909780365 1106 36-37 0.553505365166906 1106 38-39 0.5314353098212834 1106 40-41 0.5084410471196179 1106 42-43 0.2515395606447086 1106 44-45 0.36947382455660005 1106 46-47 0.5572124065806108 1106 48-49 0.6874199141134412 1106 50-51 0.5458511733722986 1106 52-53 0.5340690284584468 1106 54-55 0.5163411865478125 1106 56-57 0.21725511507094808 1106 58-59 0.2918139225703058 1106 60-61 0.6951127979265195 1106 62-63 0.5202052918853184 1106 64-65 0.4197200915171244 1106 66-67 0.33224500050703654 1106 68-69 0.23052044229125102 1106 70-71 0.45239149979669335 1106 72-73 0.24814037849133896 1106 74-75 0.4838773388615252 1106 76-77 0.05183793092201938 1106 78-79 -0.05873984360512452 1106 80-81 -0.11534464714754122 1106 82-83 0.02749308100530712 1106 84-85 0.012261449003954539 1106 86-87 0.07448033225405126 1106 88-89 -0.26228632649286965 1106 90-91 -0.2892839625832302 1106 92-93 -0.33350425047675714 1106 94-95 -0.29101375044730915 1106 96-97 -0.4296991391742573 1106 98-99 -0.6163703349221556 1106 100-101 -0.8260673179230071 1107 1 0.09820461791277069 1107 2 0.0238385401229948 1107 3 0.06587651814985662 1107 4 0.06154555808264206 1107 5 -0.0648108019885072 1107 6 -0.08757298994243712 1107 7 0.013856645553609326 1107 8 0.09902381913010316 1107 9 0.05380614914157178 1107 10-11 -0.02521869727710424 1107 12-13 0.03434028079040985 1107 14-15 0.006862020473100472 1107 16-17 -0.03781509651251724 1107 18-19 0.00511434125300525 1107 20-21 0.10991522518745711 1107 22-23 0.24304911504612647 1107 24-25 0.21082784149063372 1107 26-27 0.04359175397587478 1107 28-29 0.023609271278978383 1107 30-31 -0.023001316746963596 1107 32-33 0.05283491032889032 1107 34-35 0.023559643041330958 1107 36-37 0.06416230037719828 1107 38-39 0.14246290085250024 1107 40-41 0.22406136555862588 1107 42-43 0.17342373031376468 1107 44-45 0.05774160055977262 1107 46-47 0.07470209940830586 1107 48-49 0.06837563351074039 1107 50-51 -0.07840333640249497 1107 52-53 -0.06045331303394619 1107 54-55 -0.14510225090962336 1107 56-57 -0.2848708552889647 1107 58-59 -0.34743705862296537 1107 60-61 -0.2276806005308103 1107 62-63 -0.33632660772963874 1107 64-65 -0.3026924368043815 1107 66-67 -0.17724270517212304 1107 68-69 -0.36422150697146094 1107 70-71 -0.2922869969037727 1107 72-73 -0.41097187373414457 1107 74-75 -0.5170144017815446 1107 76-77 -0.713952316573927 1107 78-79 -0.6617866499293328 1107 80-81 -0.7590557244172871 1107 82-83 -0.6656031254600414 1107 84-85 -0.7113576065742997 1107 86-87 -0.5432333016896678 1107 88-89 -0.6516273793720728 1107 90-91 -0.6359246910805503 1107 92-93 -0.9561308151240837 1107 94-95 -1.2117686192707424 1107 96-97 -1.1680667368912019 1107 98-99 -1.2374116688497772 1107 100-101 -1.5507837933034132 1108 1 -0.1684515120043173 1108 2 -0.007616746304982769 1108 3 6.121658613551517E-4 1108 4 -0.002131074221175311 1108 5 0.06215119569684191 1108 6 0.08110578403296387 1108 7 0.13696311957966856 1108 8 0.022589825859895996 1108 9 -0.05775949515148682 1108 10-11 0.06385733618239442 1108 12-13 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-0.6729824444546679 2316 60-61 -0.8162185929201726 2316 62-63 -0.7855086375814153 2316 64-65 -0.7312938077623166 2316 66-67 -0.6345286341742273 2316 68-69 -0.6491090415060476 2316 70-71 -0.6303912435719461 2316 72-73 -0.5865481017753602 2316 74-75 -0.33021356426351645 2316 76-77 -0.40936877328188714 2316 78-79 -0.36740753401475246 2316 80-81 -0.2931281123862206 2316 82-83 -0.2934260564256199 2316 84-85 -0.34503534458059093 2316 86-87 -0.17910604933957686 2316 88-89 -0.33896795555667225 2316 90-91 -0.3044478270816633 2316 92-93 -0.3395285840594653 2316 94-95 -0.4082447882339224 2316 96-97 -0.35405503422747486 2316 98-99 -0.5962288483948761 2316 100-101 -0.2929781079113276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5967.0 3 1876.0 4 1804.0 5 2002.0 6 2377.0 7 2955.0 8 3675.0 9 4641.0 10 5884.0 11 7337.0 12 9241.0 13 11283.0 14 13422.0 15 15974.0 16 19140.0 17 24035.0 18 23447.0 19 19793.0 20 19764.0 21 21106.0 22 22960.0 23 25674.0 24 28567.0 25 31592.0 26 35226.0 27 40793.0 28 46674.0 29 54290.0 30 63178.0 31 75115.0 32 91728.0 33 114021.0 34 148851.0 35 198884.0 36 243320.0 37 262806.0 38 139213.0 39 8727.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.50975034857523 18.502514295379292 14.336958949438703 22.650776406606774 2 15.642400612225336 19.68869879910896 40.730370091947535 23.938530496718172 3 19.542455100987542 24.313937451479575 29.45509665051849 26.68851079701439 4 12.653525365154595 16.533367444058772 36.697792703629105 34.11531448715753 5 13.533526302173584 37.46632672043545 34.00736839422048 14.992778583170493 6 32.1782449403527 35.81327237078679 17.249580340121113 14.758902348739394 7 28.59781758876415 33.170026599695724 20.066125130260435 18.16603068127969 8 26.71089031786968 34.781652493322305 19.25034302580995 19.257114162998064 9 26.625073570137946 16.758979350501686 20.068578749584052 36.54736832977631 10-11 25.258198409085985 26.711207924225675 26.543148665929735 21.48744500075861 12-13 26.208874521651783 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29.70773692991716 21.976333217120505 38-39 21.75209569292994 26.4898673422855 29.694660870902716 22.063376093881846 40-41 22.067899813625136 26.44199495309214 29.71333151706757 21.776773716215157 42-43 21.976983614779822 26.37356655390391 29.48890061864694 22.16054921266933 44-45 21.840459800863794 26.70657910875811 29.530649284329712 21.922311806048384 46-47 22.219291587311602 26.851993519264965 28.86082640474373 22.067888488679703 48-49 22.02153230210858 27.030942970348608 28.769757190950052 22.177767536592754 50-51 22.215689927653106 27.290939794690374 28.524839388598895 21.96853088905763 52-53 22.427793582397783 27.0692982351648 28.54404615839442 21.958862024042997 54-55 21.986269227238523 27.161210255538165 28.718182293298472 22.13433822392484 56-57 22.10801007442109 27.420120307192413 28.373632912807743 22.098236705578753 58-59 22.086382969389863 27.012258839668373 28.510149266422 22.391208924519766 60-61 21.90803239557431 27.218450271623762 28.738888118734675 22.134629214067253 62-63 21.98765833942166 27.08866682661505 28.82130859079878 22.10236624316451 64-65 21.759315292336975 27.120196141344156 28.780628470008164 22.33986009631071 66-67 21.75317818408666 27.260739976996206 28.587681923827553 22.398399915089577 68-69 22.144154601513442 26.961910506033636 28.692852285366932 22.201082607085993 70-71 21.939766817454977 27.080966688014428 28.46164782151286 22.51761867301774 72-73 21.96738988562197 27.202598973906817 28.283852924116438 22.546158216354776 74-75 21.946860769323663 27.51457692397255 28.162990778102042 22.37557152860175 76-77 22.10243463223339 27.212363147701673 28.175804410784565 22.509397809280372 78-79 22.145647868304984 27.347430931478872 28.15244074774735 22.354480452468795 80-81 22.27471664113689 27.463596379374106 27.94395144631735 22.317735533171653 82-83 22.25217569507593 27.501375925619616 27.89797819472603 22.348470184578424 84-85 22.219042851925835 27.494746143223804 27.88005153287007 22.40615947198029 86-87 22.41084739742649 27.350256357639687 27.795183524673224 22.443712720260606 88-89 22.336126287988307 27.37002577736997 27.632484794384986 22.661363140256736 90-91 22.286314093037408 27.45334297556377 27.720635791117644 22.539707140281177 92-93 22.443915469747047 27.140194125527717 27.773753886275614 22.642136518449618 94-95 22.275868046620857 27.03549111435544 27.847347308032973 22.841293530990733 96-97 22.586425437408593 26.636124803640104 27.838389036346893 22.93906072260441 98-99 22.362979921649423 26.829466445902845 27.47957149286932 23.327982139578417 100-101 23.347254027832356 25.763194623233375 27.61267767855933 23.276873670374936 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2735.0 1 1755.0 2 1203.0 3 1544.5 4 1146.5 5 964.0 6 1716.5 7 3601.0 8 5654.0 9 6107.0 10 4597.5 11 3016.5 12 2696.5 13 2790.5 14 2844.5 15 2884.0 16 2912.0 17 3090.5 18 3354.5 19 3528.5 20 3839.0 21 4495.5 22 5260.0 23 6418.5 24 7858.0 25 9525.0 26 11818.5 27 14410.0 28 16776.0 29 19136.5 30 21827.5 31 25246.0 32 30067.5 33 33044.5 34 34165.0 35 36595.0 36 40055.0 37 43342.0 38 46203.0 39 48589.0 40 50923.5 41 54231.0 42 56392.5 43 57673.0 44 59655.5 45 62975.0 46 64961.5 47 65358.5 48 66562.5 49 66327.0 50 64684.0 51 63319.5 52 62564.0 53 62377.5 54 62259.5 55 59221.0 56 54701.5 57 50362.5 58 46173.0 59 40932.0 60 34228.0 61 28920.5 62 24060.5 63 18334.5 64 13259.0 65 10062.0 66 7711.0 67 5951.5 68 4623.0 69 3476.5 70 2582.0 71 1854.0 72 1385.0 73 1112.5 74 854.0 75 634.0 76 422.5 77 253.0 78 176.5 79 131.0 80 74.5 81 39.5 82 23.0 83 19.5 84 13.0 85 6.5 86 6.0 87 4.5 88 5.0 89 3.5 90 3.0 91 3.0 92 2.5 93 2.5 94 1.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1456146181919753 2 0.05212895067616067 3 0.14929558251801778 4 0.1282924331282459 5 0.19059816752934755 6 0.19417086819874177 7 0.16023021183949698 8 0.0688015538000002 9 0.11714127649347009 10-11 0.10079346433957545 12-13 0.14685965024343084 14-15 0.13329962724823016 16-17 0.13159447465601928 18-19 0.12915854238143235 20-21 0.1305389040036983 22-23 0.1884328943963814 24-25 0.20924658238701876 26-27 0.14407186108473688 28-29 0.12385362320566523 30-31 0.09719369775601919 32-33 0.12106583404697126 34-35 0.09215943772187284 36-37 0.08609667294956753 38-39 0.0506673913114085 40-41 0.0575421335085761 42-43 0.06146669106207729 44-45 0.03878545499425661 46-47 0.05123577550881212 48-49 0.041546178238788485 50-51 0.05196655519118821 52-53 0.02619980490889072 54-55 0.04901637054752179 56-57 0.0263351344797011 58-59 0.04755481118276962 60-61 0.036268324977183436 62-63 0.04942235925995295 64-65 0.0386771913376083 66-67 0.037350961543666523 68-69 0.062278668486939616 70-71 0.050586193568922265 72-73 0.04985541388654618 74-75 0.041086057698033175 76-77 0.019974644651612965 78-79 0.022085785956254985 80-81 0.025929145767269946 82-83 0.02184219272879629 84-85 0.019785183252478425 86-87 0.022221115527065374 88-89 0.06219747074445338 90-91 0.10758700879425682 92-93 0.07702959170527168 94-95 0.07508084588560213 96-97 0.06723173077859974 98-99 0.055485124032258235 100-101 0.1281300376432734 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1847342.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.99718910684703 #Duplication Level Percentage of deduplicated Percentage of total 1 80.72266932844134 50.04578595564858 2 11.69080581802611 14.495941982231844 3 3.41042703158444 6.343106688367304 4 1.43963767335852 3.5701395632219772 5 0.7506125650057283 2.326793456931782 6 0.43128541256987635 1.60430899692715 7 0.2802374948536073 1.2161755874287714 8 0.1985559759534061 0.9847929911582312 9 0.1475282531232193 0.8231703306734719 >10 0.846907390596129 9.590041957622798 >50 0.05281578302261594 2.2255551771066564 >100 0.024419139639568373 2.873359303411386 >500 0.0023542470912535536 1.0234808300929794 >1k 0.0014823037241226073 1.395292014235984 >5k 1.743886734261891E-4 0.8469272785647655 >10k+ 8.719433671309455E-5 0.635127886376296 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11749 0.6359948509804898 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9528 0.5157680602725428 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6139 0.33231529408198374 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2534 0.1371700529734072 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2414 0.13067423357450866 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1911 0.10344592392745901 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 5.413182832415438E-5 5 0.0 0.0 0.0 0.0 5.413182832415438E-5 6 0.0 0.0 0.0 0.0 5.413182832415438E-5 7 5.413182832415438E-5 0.0 0.0 0.0 5.413182832415438E-5 8 5.413182832415438E-5 0.0 0.0 0.0 5.413182832415438E-5 9 5.413182832415438E-5 0.0 0.0 0.0 5.413182832415438E-5 10-11 8.119774248623157E-5 0.0 0.0 0.0 5.413182832415438E-5 12-13 1.8946139913454034E-4 0.0 0.0 0.0 5.413182832415438E-5 14-15 2.706591416207719E-4 0.0 0.0 0.0 5.413182832415438E-5 16-17 2.706591416207719E-4 0.0 0.0 2.706591416207719E-5 5.413182832415438E-5 18-19 2.977250557828491E-4 0.0 0.0 1.0826365664830876E-4 1.0826365664830876E-4 20-21 3.247909699449263E-4 0.0 0.0 1.6239548497246314E-4 1.0826365664830876E-4 22-23 3.247909699449263E-4 0.0 0.0 2.706591416207719E-4 1.894613991345403E-4 24-25 4.33054626593235E-4 0.0 0.0 6.766478540519297E-4 2.706591416207719E-4 26-27 4.33054626593235E-4 0.0 0.0 0.0016780866780487858 2.706591416207719E-4 28-29 4.33054626593235E-4 0.0 0.0 0.0044929417509048135 2.706591416207719E-4 30-31 4.33054626593235E-4 0.0 0.0 0.014101341278442215 2.706591416207719E-4 32-33 4.33054626593235E-4 0.0 0.0 0.03331814033351702 2.706591416207719E-4 34-35 4.33054626593235E-4 0.0 0.0 0.06230573440110169 2.706591416207719E-4 36-37 4.33054626593235E-4 0.0 0.0 0.1079659315925259 2.706591416207719E-4 38-39 4.871864549173894E-4 0.0 0.0 0.1771464081907952 2.706591416207719E-4 40-41 5.142523690794666E-4 0.0 0.0 0.2491958716902447 2.706591416207719E-4 42-43 6.225160257277754E-4 0.0 0.0 0.31840341420267604 2.706591416207719E-4 44-45 7.03713768214007E-4 0.0 0.0 0.40255134133257403 2.706591416207719E-4 46-47 7.849115107002385E-4 0.0 0.0 0.5021809713631802 2.706591416207719E-4 48-49 8.119774248623157E-4 0.0 0.0 0.6069260591704189 2.706591416207719E-4 50-51 8.119774248623157E-4 0.0 0.0 0.721956194359247 2.706591416207719E-4 52-53 8.119774248623157E-4 0.0 0.0 0.8258081069991372 2.706591416207719E-4 54-55 8.6610925318647E-4 0.0 0.0 0.9384293758275404 3.247909699449263E-4 56-57 8.6610925318647E-4 0.0 0.0 1.0672089954107036 3.518568841070035E-4 58-59 8.6610925318647E-4 0.0 0.0 1.207951749053505 3.789227982690807E-4 60-61 8.6610925318647E-4 0.0 0.0 1.3622545256915068 3.789227982690807E-4 62-63 8.6610925318647E-4 0.0 0.0 1.511577174123687 4.871864549173894E-4 64-65 8.6610925318647E-4 0.0 0.0 1.6613599430966222 4.871864549173894E-4 66-67 9.202410815106244E-4 0.0 0.0 1.83306610254084 4.871864549173894E-4 68-69 9.202410815106244E-4 0.0 0.0 2.0180886917527996 4.871864549173894E-4 70-71 9.202410815106244E-4 0.0 0.0 2.2140188443720765 4.871864549173894E-4 72-73 9.743729098347788E-4 0.0 0.0 2.4101925902188115 5.954501115656982E-4 74-75 0.0010555706523210104 0.0 0.0 2.607124181662085 5.954501115656982E-4 76-77 0.0010826365664830875 0.0 0.0 2.814043095431165 5.954501115656982E-4 78-79 0.0010826365664830875 0.0 0.0 3.051627689945879 5.954501115656982E-4 80-81 0.0012450320514555506 0.0 0.0 3.306209678554377 5.954501115656982E-4 82-83 0.0012450320514555506 0.0 0.0 3.5675040138750704 5.954501115656982E-4 84-85 0.0012720979656176278 0.0 0.0 3.818296774500878 5.954501115656982E-4 86-87 0.0012991638797797051 0.0 0.0 4.0854644131947415 5.954501115656982E-4 88-89 0.0012991638797797051 0.0 0.0 4.366706327252885 5.954501115656982E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4755 0.0 58.37989 1 GTATCAA 8475 0.0 40.957462 1 ATCAACG 10840 0.0 32.158016 3 TATCAAC 10855 0.0 31.93747 2 TCAACGC 11015 0.0 31.517586 4 CAACGCA 11295 0.0 30.778376 5 AACGCAG 11630 0.0 29.884512 6 ACGCAGA 13170 0.0 26.205982 7 CGCAGAG 13145 0.0 26.106915 8 TGGTATC 1975 0.0 26.005156 2 GTGGTAT 2190 0.0 24.785973 1 GTGTAGC 1305 0.0 24.446096 1 GCAGAGT 14080 0.0 23.899992 9 GAGTACT 9000 0.0 23.793951 12-13 GTAAGGT 765 0.0 23.623093 4 GTACATG 8410 0.0 23.156479 1 TACATGG 8345 0.0 22.965794 2 TAAGGTG 940 0.0 22.766655 5 GTACTTT 9530 0.0 22.22098 14-15 CAGAGTA 13720 0.0 21.980263 10-11 >>END_MODULE