FastQCFastQC Report
Thu 26 May 2016
SRR938160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847342
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113890.6165073927837942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97800.5294092810102298No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63720.3449280100815117No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25290.13689939383178643No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24360.13186513379764006No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21140.11443468507726237No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA18590.10063106885460299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49550.053.786581
GTATCAA86950.040.158041
ATCAACG108200.031.7804623
TATCAAC110450.031.3540062
TCAACGC110100.031.229494
CAACGCA111900.030.727145
AACGCAG114950.029.9944786
TGGTATC21250.027.9435982
ACGCAGA129850.026.3705237
CGCAGAG130200.026.2631578
GTGGTAT24250.025.4675031
GCAGAGT141750.024.0226829
GTAAGGT8850.023.0748484
TAAGGTG9700.023.0112295
TACATGG81100.022.9027252
GAGTACT89900.022.74376712-13
GTACATG82750.022.7343181
GTACTTT92350.021.90836114-15
ACATGGG81550.021.72373
CAGAGTA137850.021.58526410-11
Produced by FastQC (version 0.11.5)