##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938160_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1847342 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53150147617496 34.0 31.0 34.0 31.0 34.0 2 32.73675258831337 34.0 31.0 34.0 31.0 34.0 3 32.786450478579496 34.0 31.0 34.0 31.0 34.0 4 36.24035343753349 37.0 35.0 37.0 35.0 37.0 5 36.217505475434436 37.0 37.0 37.0 35.0 37.0 6 36.25748616119809 37.0 37.0 37.0 35.0 37.0 7 36.21533424780036 37.0 37.0 37.0 35.0 37.0 8 36.204385002885225 37.0 37.0 37.0 35.0 37.0 9 37.8955185341967 39.0 38.0 39.0 35.0 39.0 10-11 37.875173086521066 39.0 38.0 39.0 35.0 39.0 12-13 37.79163657839209 39.0 38.0 39.0 35.0 39.0 14-15 39.06473896008427 40.0 38.5 41.0 36.0 41.0 16-17 38.950105069878774 40.0 38.0 41.0 35.5 41.0 18-19 39.207742258877886 40.5 39.0 41.0 36.0 41.0 20-21 39.19585896926503 41.0 39.0 41.0 36.0 41.0 22-23 39.09771092737566 40.0 39.0 41.0 35.5 41.0 24-25 38.99428557354296 40.0 38.5 41.0 35.0 41.0 26-27 38.8199174814409 40.0 38.0 41.0 35.0 41.0 28-29 38.697717314931396 40.0 38.0 41.0 34.5 41.0 30-31 38.44010800382387 40.0 38.0 41.0 34.0 41.0 32-33 38.209896164326906 40.0 38.0 41.0 34.0 41.0 34-35 38.04460435587996 40.0 37.0 41.0 34.0 41.0 36-37 38.0330377374628 40.0 38.0 41.0 33.5 41.0 38-39 37.88318405579476 40.0 37.0 41.0 33.0 41.0 40-41 37.700830977696604 40.0 37.0 41.0 33.0 41.0 42-43 37.45877644745802 40.0 37.0 41.0 33.0 41.0 44-45 37.16738319163425 40.0 36.0 41.0 32.0 41.0 46-47 36.75323735399293 40.0 36.0 41.0 31.0 41.0 48-49 36.34163733623768 39.0 35.0 41.0 30.0 41.0 50-51 35.960120811414455 39.0 35.0 40.5 29.0 41.0 52-53 35.44702578082456 38.5 35.0 40.0 27.5 41.0 54-55 35.13622112202289 38.0 34.5 40.0 27.0 41.0 56-57 34.79826258483811 38.0 34.0 40.0 26.0 41.0 58-59 34.35967676802671 37.5 34.0 40.0 25.5 41.0 60-61 33.8918781687419 37.0 33.0 40.0 23.5 41.0 62-63 33.435262122552295 36.5 33.0 39.0 22.5 41.0 64-65 32.95402854479571 36.0 32.0 39.0 21.5 40.0 66-67 32.41224283321659 35.0 31.5 39.0 19.5 40.0 68-69 31.84372547151529 35.0 31.0 38.0 17.5 40.0 70-71 31.241211697671574 35.0 30.5 37.5 12.5 39.5 72-73 30.6567270705695 34.5 30.0 37.0 8.0 39.0 74-75 30.070587904134698 34.0 29.0 36.0 7.0 39.0 76-77 28.712314233098148 32.5 27.5 35.0 4.0 37.0 78-79 29.400940919439932 34.0 29.0 35.0 2.0 37.0 80-81 29.07839479641561 34.0 29.0 35.0 2.0 37.0 82-83 28.52072085190506 34.0 28.0 35.0 2.0 36.5 84-85 27.931234443865836 33.0 26.5 35.0 2.0 36.0 86-87 27.29244747318039 33.0 26.0 35.0 2.0 35.5 88-89 26.551912694022004 32.0 25.0 35.0 2.0 35.0 90-91 25.997772475264462 32.0 23.5 34.5 2.0 35.0 92-93 25.188872986160657 31.5 19.5 34.0 2.0 35.0 94-95 24.36511728743243 31.0 14.0 34.0 2.0 35.0 96-97 23.317175433677143 31.0 2.0 34.0 2.0 35.0 98-99 21.88285520493769 30.0 2.0 34.0 2.0 35.0 100-101 17.529052552261575 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.009490496056372422 1101 2 0.07299453483589247 1101 3 -0.007454714444669719 1101 4 -0.01729550721542239 1101 5 -0.07798530747738397 1101 6 0.03284194911907434 1101 7 0.010630342360379075 1101 8 0.030379974225262174 1101 9 -0.16481356343349773 1101 10-11 -0.039225045639923906 1101 12-13 0.04696788474264224 1101 14-15 0.13384385706339685 1101 16-17 0.11427219947854184 1101 18-19 0.07927562116776699 1101 20-21 0.15075372307401125 1101 22-23 0.14762681241679587 1101 24-25 0.13501856840908033 1101 26-27 0.042295234081656474 1101 28-29 0.02893233155850794 1101 30-31 0.13074468251004134 1101 32-33 0.07587923858623924 1101 34-35 0.10808772422210922 1101 36-37 0.13276098140116943 1101 38-39 0.07610147643964638 1101 40-41 0.11121899750850872 1101 42-43 0.1953130434863013 1101 44-45 0.2456207332104441 1101 46-47 0.03006143122816951 1101 48-49 0.07758352135240187 1101 50-51 0.08918428985763427 1101 52-53 -0.08089920419167385 1101 54-55 -0.25008613316912687 1101 56-57 -0.10397402605343586 1101 58-59 -0.09897234773912089 1101 60-61 -0.06296423853304134 1101 62-63 -0.02005574204416405 1101 64-65 -0.023498929758240195 1101 66-67 -0.37122375593431656 1101 68-69 -0.10716883321100212 1101 70-71 -0.10785252729299444 1101 72-73 -0.13779302323498044 1101 74-75 -0.4295886607515804 1101 76-77 -0.3126717710473095 1101 78-79 -0.355953635502015 1101 80-81 -0.14719572709034878 1101 82-83 -0.005524233342253382 1101 84-85 -0.16940696857573911 1101 86-87 -0.07819843583247277 1101 88-89 0.03725971991353205 1101 90-91 -0.4343487464044671 1101 92-93 -0.5685712904824705 1101 94-95 -0.6751930294218234 1101 96-97 -0.6332235638770563 1101 98-99 -0.9453912898338395 1101 100-101 -0.8140229529804053 1102 1 0.14611589848875184 1102 2 0.12451089012704131 1102 3 0.14286463790063664 1102 4 0.1631926108990811 1102 5 0.09326404739658045 1102 6 0.06002738087690318 1102 7 0.12907348870886892 1102 8 0.13861397609662873 1102 9 0.23614134275500476 1102 10-11 0.12661920996639253 1102 12-13 0.1149934839819764 1102 14-15 0.05002513369105088 1102 16-17 0.08975920482540545 1102 18-19 0.23223171430841205 1102 20-21 0.09758058041879991 1102 22-23 0.16111902025146918 1102 24-25 0.17834172330871922 1102 26-27 0.15473462015300044 1102 28-29 0.2585391696153252 1102 30-31 0.16447051570976612 1102 32-33 0.060796052225974506 1102 34-35 0.3120755802977797 1102 36-37 0.2160775633937959 1102 38-39 0.22578427783814448 1102 40-41 0.2536941357270095 1102 42-43 0.10830697870328265 1102 44-45 0.01643168156765995 1102 46-47 0.12221255065645664 1102 48-49 0.10837866761154658 1102 50-51 0.19955687955906853 1102 52-53 -0.011202658716960912 1102 54-55 0.03284660381647342 1102 56-57 0.06743506481939932 1102 58-59 0.17334622128158372 1102 60-61 -0.03295979543934635 1102 62-63 0.16352918047290643 1102 64-65 0.33670754248119295 1102 66-67 0.089836157229918 1102 68-69 0.004705992794423963 1102 70-71 -0.09684211035212442 1102 72-73 -0.1455654459016209 1102 74-75 -0.2663506345700348 1102 76-77 -0.27165471738478786 1102 78-79 -0.2324384224927627 1102 80-81 -0.12168901897607753 1102 82-83 -0.07012717471814156 1102 84-85 -0.37899156723355887 1102 86-87 -0.6158508630618549 1102 88-89 -0.56277789083477 1102 90-91 -0.6018898729401059 1102 92-93 -0.5272034557687455 1102 94-95 -0.527567053357096 1102 96-97 -0.9107703459843854 1102 98-99 -0.7351717355949887 1102 100-101 -1.1482937631310755 1103 1 0.16852032703221909 1103 2 0.1379170276671502 1103 3 0.07907309499687187 1103 4 0.1099126346555579 1103 5 0.058037644710779546 1103 6 0.12001436280682753 1103 7 0.08960009064013263 1103 8 0.07275086530280106 1103 9 0.2076496916422812 1103 10-11 0.12589369252435034 1103 12-13 0.15092215755611704 1103 14-15 0.17519151527498167 1103 16-17 0.1379457486702833 1103 18-19 0.11787779227735484 1103 20-21 0.15158907365967877 1103 22-23 0.22812214953756182 1103 24-25 0.24213495346235447 1103 26-27 0.24582024170960182 1103 28-29 0.23058462637635557 1103 30-31 0.1816814016959114 1103 32-33 0.13076874127482796 1103 34-35 0.2870356674648562 1103 36-37 0.1980796690816291 1103 38-39 0.22223092962201463 1103 40-41 0.25372694336327584 1103 42-43 0.3162905201432622 1103 44-45 0.3888530950931042 1103 46-47 0.3598118265637993 1103 48-49 0.2957828224455952 1103 50-51 0.30125717748990155 1103 52-53 0.21370231162118358 1103 54-55 0.15081780208632978 1103 56-57 0.14051829273085303 1103 58-59 0.24346907556844144 1103 60-61 0.14877126293125542 1103 62-63 0.13152936194600073 1103 64-65 -0.09054311760844769 1103 66-67 -0.10094268480674629 1103 68-69 0.13154725107787968 1103 70-71 -0.0607364630143401 1103 72-73 -0.27594168012489817 1103 74-75 -0.24366406035466426 1103 76-77 -0.15815998047953528 1103 78-79 -0.4329410729748311 1103 80-81 -0.27591265532199216 1103 82-83 -0.2454479959064031 1103 84-85 -0.6269826150607223 1103 86-87 -0.7461982265652161 1103 88-89 -1.066684624161553 1103 90-91 -1.0182534595383927 1103 92-93 -0.8647205438604395 1103 94-95 -0.7616864701535562 1103 96-97 -0.9124941529300052 1103 98-99 -1.236199645707316 1103 100-101 -2.1263507036896403 1104 1 0.1248532497966579 1104 2 0.17678554429519266 1104 3 0.12868856003203888 1104 4 0.09833037474611928 1104 5 0.013293483018088637 1104 6 0.0902422021894651 1104 7 0.08538030918460748 1104 8 0.09605454513636857 1104 9 0.1449999233120849 1104 10-11 0.16470053596406586 1104 12-13 0.1303811202868843 1104 14-15 0.2796694104446189 1104 16-17 0.21387053900779307 1104 18-19 0.23809131153221585 1104 20-21 0.3067045220026685 1104 22-23 0.2702728745928553 1104 24-25 0.27166200269020635 1104 26-27 0.2776320102190297 1104 28-29 0.1337759942384693 1104 30-31 0.13559993334691 1104 32-33 0.13735160737372354 1104 34-35 0.11244150691425858 1104 36-37 0.10394160899331695 1104 38-39 0.17478210870444144 1104 40-41 0.22662676045219854 1104 42-43 0.12495498812855743 1104 44-45 0.04693131637510106 1104 46-47 0.08108819800155942 1104 48-49 8.547832839980174E-4 1104 50-51 -0.0728734677592513 1104 52-53 -0.17436647419011564 1104 54-55 -0.18608156519243835 1104 56-57 -0.1264030906461997 1104 58-59 -0.15641091648959815 1104 60-61 -0.303922766014729 1104 62-63 -0.3188393593807959 1104 64-65 -0.4100910584092148 1104 66-67 -0.45526200432833264 1104 68-69 -0.3162085301246229 1104 70-71 -0.2475557957139607 1104 72-73 -0.5467052504226686 1104 74-75 -0.5494249686900297 1104 76-77 -0.49621602752686655 1104 78-79 -0.5955367235215405 1104 80-81 -0.7938976815638483 1104 82-83 -0.8526377542915817 1104 84-85 -0.7500541858293843 1104 86-87 -0.6384052657426267 1104 88-89 -0.7883338460427076 1104 90-91 -0.5428709012783131 1104 92-93 -0.538853649008832 1104 94-95 -0.6048975874925091 1104 96-97 -0.7128713960506339 1104 98-99 -0.8322232185704088 1104 100-101 -1.6814798571855967 1105 1 0.18733494220238356 1105 2 0.11377505850588676 1105 3 0.07984573343956924 1105 4 0.05671850575343029 1105 5 -0.0019374345468605725 1105 6 0.06141278726514798 1105 7 0.047122358100502026 1105 8 0.05034878112167718 1105 9 0.10501113306456489 1105 10-11 0.08844969773480926 1105 12-13 0.06739131915990981 1105 14-15 0.09446528285361921 1105 16-17 -0.041958699096021235 1105 18-19 -0.025612277256698235 1105 20-21 0.10624862537260071 1105 22-23 0.09501890363812748 1105 24-25 0.10952027271846987 1105 26-27 0.08095923824074447 1105 28-29 0.1260904745029734 1105 30-31 0.09178865086448695 1105 32-33 0.20794635814022655 1105 34-35 0.09684493789922044 1105 36-37 -0.03415203247475773 1105 38-39 0.0920384550591038 1105 40-41 0.012941687772304533 1105 42-43 -0.06354784266756752 1105 44-45 -0.0209073699486666 1105 46-47 0.18508017101808605 1105 48-49 0.26950050506194145 1105 50-51 -0.005585427163921963 1105 52-53 0.12898644284770455 1105 54-55 0.054009647495938395 1105 56-57 -0.1126179308905435 1105 58-59 0.20928740771912402 1105 60-61 0.11534382925648856 1105 62-63 0.0847005536768961 1105 64-65 0.2964528014154979 1105 66-67 0.05695162152616007 1105 68-69 -0.10594960485964222 1105 70-71 -0.0356037307507755 1105 72-73 -0.27546794062981306 1105 74-75 -0.2541350664751043 1105 76-77 -0.24779490114691427 1105 78-79 -0.43821185088234316 1105 80-81 -0.5644687450557484 1105 82-83 -0.4963250956118692 1105 84-85 -0.5585572501620213 1105 86-87 -0.7966257508980448 1105 88-89 -0.7459786637007362 1105 90-91 -0.7904863266984279 1105 92-93 -0.6478836717953236 1105 94-95 -0.8400199773049764 1105 96-97 -1.05062057116632 1105 98-99 -1.1056551043110048 1105 100-101 -1.7469039428067532 1106 1 0.15264163976320333 1106 2 0.22230017566362648 1106 3 0.19813173400317652 1106 4 0.15947810538186502 1106 5 0.18080045567451464 1106 6 0.1322449464118023 1106 7 0.15172412616470865 1106 8 0.15207614019318783 1106 9 0.07101712402593563 1106 10-11 0.1710409450863679 1106 12-13 0.10450292429919017 1106 14-15 0.21945615029054721 1106 16-17 0.1935121758557088 1106 18-19 0.21779662151276113 1106 20-21 0.2768516876514653 1106 22-23 0.34124689416113796 1106 24-25 0.341919475315251 1106 26-27 0.3559777642972506 1106 28-29 0.24567121157908645 1106 30-31 0.30907424437670983 1106 32-33 0.4619850384587707 1106 34-35 0.4350347073411456 1106 36-37 0.40786871670852776 1106 38-39 0.27033844149072195 1106 40-41 0.3667408340081195 1106 42-43 0.27078798516013336 1106 44-45 0.1749761924815374 1106 46-47 0.2961796272391837 1106 48-49 0.3642839850381492 1106 50-51 0.2671823134921567 1106 52-53 0.48326309166673553 1106 54-55 0.27878342086224706 1106 56-57 0.2112358019793774 1106 58-59 0.2376594109337944 1106 60-61 0.06629883442290918 1106 62-63 0.3398690625120011 1106 64-65 0.17092807043471936 1106 66-67 -0.0490247277448006 1106 68-69 -0.023593716704841228 1106 70-71 -0.4946658426168433 1106 72-73 -0.35347881620777244 1106 74-75 -0.19318903731352677 1106 76-77 -0.1308730445751216 1106 78-79 -0.0909657100425676 1106 80-81 -0.22031306355780345 1106 82-83 -0.3383839406296616 1106 84-85 -0.27648212308892894 1106 86-87 -0.24836876579194822 1106 88-89 -0.350935297072688 1106 90-91 -0.6691526593742161 1106 92-93 -0.7151708308897895 1106 94-95 -0.8401447020234372 1106 96-97 -0.9283475544980888 1106 98-99 -0.9835240337658675 1106 100-101 -1.4307027759261253 1107 1 0.0789190481748463 1107 2 0.04349426002767842 1107 3 0.08169442513467828 1107 4 0.019978421097050614 1107 5 -0.03494847027479864 1107 6 -0.023890142545596404 1107 7 -7.16785571228229E-4 1107 8 0.05728698841465274 1107 9 0.06516091681772451 1107 10-11 0.09889414371455274 1107 12-13 0.030527451035936792 1107 14-15 0.05089983536774412 1107 16-17 0.005729889334887162 1107 18-19 0.14159241569122116 1107 20-21 0.047539853903366236 1107 22-23 -0.01174001913480538 1107 24-25 0.049278889199285913 1107 26-27 -0.01837238860795054 1107 28-29 -0.011290463073315493 1107 30-31 0.046445327341714915 1107 32-33 -0.06291374696633767 1107 34-35 0.05466816819865272 1107 36-37 -0.04523813740629379 1107 38-39 -0.11836839529072307 1107 40-41 -0.2095642768636239 1107 42-43 -0.12411538123044608 1107 44-45 -0.05213074210971058 1107 46-47 -0.08491661636912795 1107 48-49 -0.05678350372610197 1107 50-51 -0.14099532892015532 1107 52-53 -0.053908812157750674 1107 54-55 -0.10973832015225327 1107 56-57 -0.17253690165691893 1107 58-59 -0.18373632876832602 1107 60-61 -0.14045499439755105 1107 62-63 -0.10042424308792164 1107 64-65 -0.26562431971497347 1107 66-67 -0.24458490553578116 1107 68-69 -0.2959022447292021 1107 70-71 -0.49544290436083216 1107 72-73 -0.7222997747438491 1107 74-75 -0.49474594652374293 1107 76-77 -0.5858740350541431 1107 78-79 -0.5481816592309947 1107 80-81 -0.5385784252683585 1107 82-83 -0.4720684297282034 1107 84-85 -0.4554234557784511 1107 86-87 -0.6116797204899171 1107 88-89 -0.6417515446429185 1107 90-91 -0.6372386515246689 1107 92-93 -0.6132355277413026 1107 94-95 -0.6542543851939584 1107 96-97 -0.6950088044826117 1107 98-99 -0.7045293080666504 1107 100-101 -1.283240723653595 1108 1 -0.05578385667315189 1108 2 -0.12747754404232126 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52-53 -0.2947080597816054 2316 54-55 -0.17854880997866474 2316 56-57 -0.08809679635901091 2316 58-59 -0.07615718157713758 2316 60-61 0.11469956416789984 2316 62-63 -0.04138791009631149 2316 64-65 0.12650353210341336 2316 66-67 0.1892737589531066 2316 68-69 0.3769811543429178 2316 70-71 0.42821415633316207 2316 72-73 0.6578578246028179 2316 74-75 0.7722268254524174 2316 76-77 0.6926107002241331 2316 78-79 0.5843620845788458 2316 80-81 0.6867934188351299 2316 82-83 0.9404915171015666 2316 84-85 0.7317885388002701 2316 86-87 0.9836146705719244 2316 88-89 1.132686128030013 2316 90-91 1.252448973364558 2316 92-93 1.4617234217122252 2316 94-95 1.7562958224479601 2316 96-97 1.8984541674900761 2316 98-99 2.305073044629218 2316 100-101 2.5542783898283616 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 8.0 4 15.0 5 34.0 6 76.0 7 219.0 8 456.0 9 857.0 10 1488.0 11 2469.0 12 3734.0 13 5887.0 14 7716.0 15 10788.0 16 14232.0 17 20268.0 18 20260.0 19 15699.0 20 14656.0 21 14997.0 22 16044.0 23 17456.0 24 19659.0 25 22638.0 26 25842.0 27 30359.0 28 36534.0 29 44253.0 30 53274.0 31 64782.0 32 81205.0 33 104643.0 34 142756.0 35 206133.0 36 283378.0 37 354766.0 38 199084.0 39 10672.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78668785943478 18.8346937018825 14.728793381879642 22.64982505680308 2 15.89916002782763 19.943153671866753 40.13247901423053 24.025207286075087 3 19.604259488839684 24.222892316354205 29.695804934034225 26.477043260771882 4 12.885323886968411 16.81805534654655 36.46108841784575 33.83553234863928 5 13.465452655739124 37.4485741506615 34.145573046359054 14.940400147240327 6 32.27982575849027 36.01382759141481 16.973011187552135 14.733335462542785 7 28.655387037159336 33.09387216877005 19.972912433106593 18.277828360964023 8 26.70263546219379 35.02058633431167 19.06154897144113 19.215229232053403 9 26.405126933724237 16.703350002327667 20.045719742202582 36.845803321745514 10-11 25.3046809957225 26.740230016964915 26.457959598168614 21.49712938914397 12-13 26.144435482561317 24.035148024446094 27.408394818467734 22.412021674524855 14-15 22.771094902838783 25.420847899306136 26.052918192733127 25.75513900512195 16-17 21.922925662367316 28.686330198027438 26.947868896919253 22.442875242685993 18-19 21.814017112153568 26.66133287718246 29.881500014615593 21.64314999604838 20-21 22.460378208258135 25.805048550836823 30.273468583510795 21.461104657394245 22-23 22.095123696640904 25.88083311049064 30.144716029841796 21.879327163026662 24-25 21.893658329821417 26.09745726355187 30.236404143061037 21.772480263565676 26-27 21.52658158286196 26.473407588782365 30.09763116184196 21.902379666513717 28-29 21.552394295440077 26.680196879209017 30.074343988601278 21.69306483674963 30-31 21.67519858256425 26.348719833679745 30.03828061752431 21.937800966231695 32-33 21.46299927138559 26.353025048962238 29.92155756757547 22.262418112076706 34-35 22.060280845758417 26.54470716157496 29.80878051051595 21.586231482150673 36-37 22.135331027843165 26.160682217222213 29.735301688193687 21.968685066740935 38-39 21.735046903766754 26.494145758144484 29.71104204909562 22.059765288993137 40-41 22.11230555156326 26.337506702318535 29.802520802562217 21.74766694355599 42-43 21.90718656797416 26.341636773721888 29.59955047579247 22.15162618251148 44-45 21.838349368985277 26.621518917450043 29.599906243673342 21.940225469891335 46-47 22.2379625634899 26.855491216835443 28.898616985169497 22.00792923450516 48-49 22.03956200523134 27.006438488454677 28.777293474683436 22.176706031630548 50-51 22.222408684422863 27.226627090966815 28.6083751542486 21.942589070361723 52-53 22.477230974698617 26.994196324528346 28.557515558078308 21.971057142694733 54-55 21.940824795454983 27.159507354201022 28.727764771673165 22.171903078670827 56-57 22.049940892025553 27.376913429488802 28.43990950944036 22.133236169045286 58-59 22.05402341139025 27.016280876041566 28.50592188135771 22.42377383121048 60-61 21.852564111239378 27.173854496684985 28.763382870196818 22.21019852187882 62-63 21.941996837549617 27.155031807234003 28.77429194760868 22.12867940760769 64-65 21.6770909697309 27.151907506689348 28.725894250354365 22.445107273225386 66-67 21.62481508200005 27.33649053043889 28.533419233637545 22.50527515392351 68-69 22.08319412775363 27.0168384152169 28.62473731433315 22.275230142696323 70-71 21.831770570244753 27.12275093947156 28.367520298595217 22.67795819168847 72-73 21.891779160433302 27.239240157195788 28.2151770969882 22.653803585382708 74-75 21.875559167312066 27.537249171586435 28.029871343807127 22.557320317294373 76-77 21.881405706377848 27.193005252882262 28.357876020716994 22.567713020022893 78-79 21.90493130191341 27.324595921079027 28.251588958817425 22.518883818190137 80-81 21.970447723459078 27.43201831378187 28.09374633723009 22.50378762552896 82-83 21.955723853214984 27.498256375600523 28.067425197430268 22.47859457375423 84-85 21.91421325513603 27.512111846978154 27.99103604208447 22.582638855801342 86-87 22.013229412701147 27.378310737418467 27.959101015056365 22.649358834824024 88-89 21.87088766254773 27.471828669582273 27.716961543511427 22.940322124358573 90-91 21.78808927749082 27.523318777907335 27.877029436902916 22.811562507698927 92-93 21.897629803653608 27.20307301718623 27.97625445032783 22.92304272883233 94-95 21.717422999275076 27.11233273040394 28.050996890790014 23.11924737953097 96-97 21.955771848724595 26.77442765790248 28.01443791191672 23.255362581456204 98-99 21.68449768184171 26.936246857330946 27.76262535486986 23.616630105957483 100-101 22.165074865713787 26.413645926155304 27.754207237650597 23.667071970480315 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2588.0 1 1673.5 2 1185.5 3 1528.5 4 1105.5 5 913.0 6 1826.5 7 3816.5 8 5731.0 9 6191.5 10 4664.5 11 2918.5 12 2513.0 13 2602.0 14 2783.0 15 2875.5 16 2836.5 17 2965.0 18 3139.0 19 3346.0 20 3798.0 21 4428.0 22 5185.5 23 6224.0 24 7657.0 25 9475.0 26 11730.0 27 14243.0 28 16496.0 29 18890.5 30 21421.5 31 24816.0 32 30118.0 33 33166.0 34 34316.0 35 36555.0 36 39899.0 37 43613.0 38 46466.0 39 49071.5 40 51415.0 41 54260.5 42 56249.5 43 57638.5 44 59814.5 45 63384.5 46 65416.0 47 66010.5 48 67370.0 49 66772.5 50 65186.0 51 63926.5 52 63107.0 53 63408.5 54 63670.0 55 60284.0 56 55287.5 57 50463.5 58 46378.5 59 41269.5 60 34059.0 61 28524.5 62 23652.5 63 17516.5 64 12145.0 65 9195.0 66 7003.0 67 5401.5 68 4195.5 69 2972.5 70 2156.0 71 1572.0 72 1147.0 73 926.0 74 694.0 75 509.5 76 372.0 77 202.5 78 122.0 79 90.0 80 49.0 81 22.0 82 10.5 83 5.0 84 3.0 85 1.0 86 2.0 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009256542643430399 2 0.013911879879307674 3 0.009310674471754554 4 0.0 5 0.0011909002231313963 6 0.0011367683948072418 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.495819398898526E-4 14-15 0.0 16-17 0.002571261845397333 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00327497561361134 26-27 0.01837775571605041 28-29 0.006387555742250217 30-31 0.0033832392702596486 32-33 0.0 34-35 0.009283608557592476 36-37 6.766478540519297E-4 38-39 0.0026524595878835646 40-41 0.0018134162488591718 42-43 0.003004316471990568 44-45 0.0 46-47 5.413182832415438E-5 48-49 7.578455965381614E-4 50-51 0.0043576121800944275 52-53 0.008146840162785234 54-55 0.007172467252950456 56-57 0.016158350754760083 58-59 0.022762433810306917 60-61 0.003491502926907957 62-63 0.019297996797561036 64-65 0.020353567449882048 66-67 0.012964572883634973 68-69 0.02993490106325737 70-71 0.015860625698977233 72-73 0.004953062291660126 74-75 0.015562900643194382 76-77 0.019270930883398957 78-79 0.024711179629976474 80-81 0.011286486205586189 82-83 0.03323694259103079 84-85 0.02111141304642021 86-87 0.0296642419216366 88-89 0.020813687990637358 90-91 0.026714057277970188 92-93 0.023141356608575997 94-95 0.01347882525271444 96-97 0.014101341278442215 98-99 0.010068520068292714 100-101 0.009094147158457935 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1847342.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.09897723985244 #Duplication Level Percentage of deduplicated Percentage of total 1 79.25087868711013 47.628967544549376 2 12.60632499697722 15.152544781430333 3 3.6066793477955548 6.502732201038326 4 1.5577006897770964 3.744648732056646 5 0.8033028483913747 2.413883980109093 6 0.457260836621743 1.648854516768361 7 0.3084717285460394 1.297718477511845 8 0.23135263594487543 1.1123245441624747 9 0.17017207439076346 0.920445086310207 >10 0.9165559480350185 10.070687631906987 >50 0.05931506643773537 2.3969074880641865 >100 0.027571171565217954 3.09228930713152 >500 0.002702429637128068 1.1526468867320807 >1k 0.0014412958064683025 1.3743409828838757 >5k 1.801619758085378E-4 0.8744329770558718 >10k+ 9.00809879042689E-5 0.6165748622888388 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11389 0.6165073927837942 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9780 0.5294092810102298 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6372 0.3449280100815117 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2529 0.13689939383178643 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2436 0.13186513379764006 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2114 0.11443468507726237 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1859 0.10063106885460299 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.413182832415438E-5 0.0 0.0 0.0 0.0 7 5.413182832415438E-5 0.0 0.0 0.0 0.0 8 5.413182832415438E-5 0.0 0.0 0.0 1.0826365664830876E-4 9 5.413182832415438E-5 0.0 0.0 0.0 1.0826365664830876E-4 10-11 5.413182832415438E-5 0.0 0.0 0.0 1.0826365664830876E-4 12-13 5.413182832415438E-5 0.0 0.0 0.0 1.0826365664830876E-4 14-15 1.0826365664830876E-4 0.0 0.0 0.0 1.3532957081038594E-4 16-17 1.0826365664830876E-4 0.0 0.0 2.706591416207719E-5 1.6239548497246314E-4 18-19 1.3532957081038594E-4 0.0 0.0 1.0826365664830876E-4 1.6239548497246314E-4 20-21 1.6239548497246314E-4 0.0 0.0 1.6239548497246314E-4 2.706591416207719E-4 22-23 1.6239548497246314E-4 0.0 0.0 2.706591416207719E-4 3.247909699449263E-4 24-25 2.165273132966175E-4 0.0 0.0 7.307796823760842E-4 3.789227982690807E-4 26-27 2.165273132966175E-4 0.0 0.0 0.0016780866780487858 3.789227982690807E-4 28-29 3.789227982690807E-4 0.0 0.0 0.0044929417509048135 3.789227982690807E-4 30-31 4.33054626593235E-4 0.0 0.0 0.014074275364280138 4.059887124311578E-4 32-33 4.33054626593235E-4 0.0 0.0 0.033372272161841174 4.33054626593235E-4 34-35 4.8718645491738937E-4 0.0 0.0 0.06238693214358792 4.33054626593235E-4 36-37 5.413182832415438E-4 0.0 0.0 0.10850724987576746 4.33054626593235E-4 38-39 5.954501115656982E-4 0.0 0.0 0.1780666492723058 4.33054626593235E-4 40-41 5.954501115656982E-4 0.0 0.0 0.2505762333125106 4.33054626593235E-4 42-43 5.954501115656982E-4 0.0 0.0 0.3205145555073181 4.33054626593235E-4 44-45 6.495819398898526E-4 0.0 0.0 0.4052038009204576 4.33054626593235E-4 46-47 7.578455965381614E-4 0.0 0.0 0.5054559469767915 4.33054626593235E-4 48-49 8.119774248623157E-4 0.0 0.0 0.6105799575822993 4.33054626593235E-4 50-51 8.119774248623157E-4 0.0 0.0 0.7263408724535034 4.33054626593235E-4 52-53 8.119774248623157E-4 0.0 0.0 0.8305446419775007 4.8718645491738937E-4 54-55 9.202410815106244E-4 0.0 0.0 0.9441402837157387 5.954501115656982E-4 56-57 9.202410815106244E-4 0.0 0.0 1.0740566716937092 5.954501115656982E-4 58-59 9.202410815106244E-4 0.0 0.0 1.2159091278171557 5.954501115656982E-4 60-61 9.202410815106244E-4 0.0 0.0 1.3707802886525613 5.954501115656982E-4 62-63 9.202410815106244E-4 0.0 0.0 1.5213750350503588 5.954501115656982E-4 64-65 9.202410815106244E-4 0.0 0.0 1.672592297473884 5.954501115656982E-4 66-67 9.743729098347788E-4 0.0 0.0 1.8459224117678263 6.495819398898526E-4 68-69 9.743729098347788E-4 0.0 0.0 2.033029076370266 6.495819398898526E-4 70-71 0.001001438823996856 0.0 0.0 2.2308809088950503 7.03713768214007E-4 72-73 0.0010555706523210104 0.0 0.0 2.4284891481923756 7.578455965381614E-4 74-75 0.0010826365664830875 0.0 0.0 2.6288851766483954 8.119774248623157E-4 76-77 0.0010826365664830875 0.0 0.0 2.8387542750611416 8.119774248623157E-4 78-79 0.0010826365664830875 0.0 0.0 3.079316120133684 8.119774248623157E-4 80-81 0.0011909002231313963 0.0 0.0 3.338120391351466 8.390433390243928E-4 82-83 0.0012720979656176278 0.0 0.0 3.6037452729380917 8.6610925318647E-4 84-85 0.0013803616222659368 0.0 0.0 3.859464029941397 8.6610925318647E-4 86-87 0.0014615593647521682 0.0 0.0 4.131882455982703 8.931751673485473E-4 88-89 0.0014615593647521682 0.0 0.0 4.420973485147851 9.473069956727016E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4955 0.0 53.78658 1 GTATCAA 8695 0.0 40.15804 1 ATCAACG 10820 0.0 31.780462 3 TATCAAC 11045 0.0 31.354006 2 TCAACGC 11010 0.0 31.22949 4 CAACGCA 11190 0.0 30.72714 5 AACGCAG 11495 0.0 29.994478 6 TGGTATC 2125 0.0 27.943598 2 ACGCAGA 12985 0.0 26.370523 7 CGCAGAG 13020 0.0 26.263157 8 GTGGTAT 2425 0.0 25.467503 1 GCAGAGT 14175 0.0 24.022682 9 GTAAGGT 885 0.0 23.074848 4 TAAGGTG 970 0.0 23.011229 5 TACATGG 8110 0.0 22.902725 2 GAGTACT 8990 0.0 22.743767 12-13 GTACATG 8275 0.0 22.734318 1 GTACTTT 9235 0.0 21.908361 14-15 ACATGGG 8155 0.0 21.7237 3 CAGAGTA 13785 0.0 21.585264 10-11 >>END_MODULE