FastQCFastQC Report
Thu 26 May 2016
SRR938159_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938159_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623569
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182441.123697237382581No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141900.874000427453345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93130.5736128245858353No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33400.20571962140198538No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28790.1773253862324299No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28330.17449212198557623No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16720.1029829961030298No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67750.054.184061
GTATCAA120150.042.536441
TATCAAC151650.033.532342
ATCAACG152350.033.3782733
TCAACGC154350.032.9457744
CAACGCA156750.032.3503845
AACGCAG160250.031.6972716
ACGCAGA183000.027.5682137
CGCAGAG183250.027.4787548
TGGTATC29150.026.0857892
GCAGAGT191850.026.0241189
GTGGTAT29950.025.9466271
GAGTACT107700.025.53708312-13
GTACTTT113000.024.27626814-15
GTACATG119150.023.6874221
TACATGG118450.023.3512672
AGTACTT109200.022.75031712-13
ACGTAGC1050.002150173522.702351
CAGAGTA188450.022.40925410-11
ACATGGG121500.021.9827793
Produced by FastQC (version 0.11.5)