FastQCFastQC Report
Thu 26 May 2016
SRR938159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938159_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623569
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173731.0700499947954167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147170.9064597808901254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94110.579648909285654No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34880.21483534115273203No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30290.1865642913852137No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28060.17282911905807513No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68700.051.917841
GTATCAA122150.041.9309121
TATCAAC154350.032.9677432
ATCAACG155850.032.5853923
TCAACGC158650.032.0402684
CAACGCA161950.031.4157895
AACGCAG167250.030.4414446
ACGCAGA188200.026.9013567
CGCAGAG188600.026.7939388
TGGTATC30450.025.9258422
GCAGAGT198050.025.203719
GTGGTAT32900.025.007041
GAGTACT110150.024.83559612-13
GGACCGA3500.024.4253656
GTACTTT115550.023.57219714-15
AGTACTT111650.023.18325212-13
GTACATG119400.022.9419441
TACATGG117950.022.8207842
ATGGGCG1954.1815838E-721.9458835
CAGAGTA194500.021.43934410-11
Produced by FastQC (version 0.11.5)