FastQCFastQC Report
Thu 26 May 2016
SRR938158_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938158_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621052
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185091.1417894058919762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148650.9169971105183546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94030.5800554208008133No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32640.20135072779898486No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28780.17753903021001177No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28690.17698383518850722No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16330.10073705223521516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67850.052.1510431
GTATCAA121300.042.16221
TATCAAC155950.032.454532
ATCAACG156450.032.3508073
TCAACGC157850.032.063884
CAACGCA160400.031.5541365
AACGCAG164950.030.7682786
TGGTATC30250.028.749932
GTGGTAT31950.027.7994821
ACGCAGA184650.027.3742667
ATATGCG705.7566285E-727.17239494-95
CGCAGAG185700.027.1171598
GCAGAGT193950.025.718749
GTACATG118400.025.2474351
GAGTACT110150.024.86519212-13
TACATGG118600.024.6835212
GTACTTT116000.023.46859214-15
ACATGGG119550.023.3743113
GTATAGG2252.6484486E-923.3455851
AGTACTT111550.022.82822812-13
Produced by FastQC (version 0.11.5)